##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841146.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2340958
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.96952914148823	38.0	35.0	39.0	31.0	39.0
2	36.86248664008496	39.0	35.0	39.0	33.0	39.0
3	37.0590501837282	39.0	35.0	39.0	34.0	39.0
4	37.443302699151374	39.0	35.0	40.0	34.0	41.0
5	38.00139344661459	40.0	35.0	41.0	34.0	41.0
6	38.0102470868764	40.0	35.0	41.0	35.0	41.0
7	37.960414069795355	40.0	35.0	41.0	34.0	41.0
8	37.94959926662503	40.0	35.0	41.0	34.0	41.0
9	37.915462814796335	40.0	35.0	41.0	34.0	41.0
10	37.83826066080639	39.0	35.0	41.0	34.0	41.0
11	37.80054704099774	39.0	35.0	41.0	34.0	41.0
12	37.739458375588114	39.0	35.0	41.0	34.0	41.0
13	37.674021490347116	39.0	35.0	41.0	34.0	41.0
14	37.63398147254244	39.0	35.0	41.0	34.0	41.0
15	37.58500750547425	39.0	35.0	41.0	34.0	41.0
16	37.514961823321904	39.0	35.0	41.0	34.0	41.0
17	37.38652124472118	38.0	35.0	41.0	34.0	41.0
18	37.29310393437217	38.0	35.0	40.0	34.0	41.0
19	37.15346623049196	38.0	35.0	40.0	33.0	41.0
20	37.03953979524622	37.0	35.0	40.0	33.0	41.0
21	36.70700243233753	37.0	35.0	40.0	33.0	41.0
22	36.568811144838996	37.0	35.0	40.0	33.0	41.0
23	36.41225472648377	36.0	35.0	40.0	33.0	41.0
24	36.25015228808035	36.0	35.0	40.0	32.0	41.0
25	36.13172961664812	36.0	35.0	40.0	32.0	41.0
26	35.97134442764186	35.0	35.0	39.0	32.0	41.0
27	35.89604603586786	35.0	35.0	39.0	32.0	41.0
28	35.975600223830945	35.0	35.0	39.0	33.0	41.0
29	35.97727566804028	35.0	35.0	39.0	33.0	41.0
30	35.94687320533033	35.0	35.0	39.0	33.0	41.0
31	35.83301984027693	35.0	35.0	39.0	33.0	41.0
32	35.78212636158345	35.0	35.0	38.0	33.0	41.0
33	35.73000471441657	35.0	35.0	37.0	33.0	41.0
34	35.70104697746829	35.0	35.0	37.0	33.0	41.0
35	35.58533032018939	35.0	35.0	37.0	33.0	41.0
36	35.490858085204366	35.0	35.0	37.0	33.0	41.0
37	35.44221058162907	35.0	35.0	36.0	33.0	40.0
38	35.34916676955798	35.0	35.0	36.0	33.0	40.0
39	35.23772144804461	35.0	35.0	36.0	33.0	40.0
40	35.10351738364261	35.0	35.0	36.0	33.0	40.0
41	34.969523740064126	35.0	35.0	35.0	33.0	39.0
42	34.92962591932685	35.0	35.0	35.0	33.0	39.0
43	34.87899375742293	35.0	35.0	35.0	33.0	39.0
44	34.8435608960719	35.0	35.0	35.0	33.0	39.0
45	34.78932552161295	35.0	35.0	35.0	33.0	39.0
46	34.704949957157496	35.0	35.0	35.0	33.0	38.0
47	34.67122815993945	35.0	35.0	35.0	33.0	37.0
48	34.60305174096869	35.0	34.0	35.0	33.0	37.0
49	34.58504725394933	35.0	34.0	35.0	32.0	37.0
50	34.88867335849586	35.0	34.0	37.0	32.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	3.0
15	31.0
16	13.0
17	0.0
18	0.0
19	2.0
20	2.0
21	12.0
22	72.0
23	192.0
24	503.0
25	1100.0
26	2434.0
27	4798.0
28	9231.0
29	16263.0
30	28056.0
31	46487.0
32	77986.0
33	153206.0
34	454055.0
35	208984.0
36	302594.0
37	370242.0
38	298404.0
39	236828.0
40	129459.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	47.20687000791983	17.132174092828663	35.6609558992515
2	31.607060015600453	17.408385797609355	28.62499028175644	22.359563905033752
3	33.18572994474911	13.834507069328028	22.30813196990292	30.671631016019933
4	22.946075922763246	16.814910818562314	29.210220772863078	31.028792485811362
5	27.004286279377933	19.713766757028534	28.413495671430244	24.868451292163293
6	29.03260118293451	22.57187014888776	22.588743582755434	25.80678508542229
7	32.649966381284926	20.876794884829202	21.63699647751049	24.83624225637538
8	31.403553587890087	17.443585062183946	25.381916292389693	25.77094505753627
9	26.67331067024697	20.093141354949555	24.707790571210587	28.525757403592888
10	30.82908792041549	18.49136122903529	24.029563964838328	26.64998688571089
11	32.819982246584516	17.428505765588277	25.68704778129296	24.064464206534247
12	28.74784596733474	16.29986526883438	24.89352649641728	30.0587622674136
13	25.24680066878603	16.644339625059484	30.16974247295338	27.93911723320111
14	25.30540915300488	20.580249624299114	22.032133852892706	32.0822073698033
15	24.68250177918613	21.266079955300352	24.843504240571594	29.20791402494193
16	27.132609811880435	22.318469617994	26.61316435408068	23.935756216044883
17	29.36097102126565	20.131459000973106	20.34282545863702	30.16474451912422
18	28.318192808243463	19.93376216061971	20.70336161520198	31.04468341593484
19	28.464372278357835	18.553686140460442	26.616838063732885	26.36510351744884
20	31.514063900334822	17.354262656570516	22.351703875080204	28.77996956801446
21	29.224061260389977	19.049508790845458	23.285723195375567	28.440706753388994
22	30.138473223355568	19.483049247359414	25.68700506373886	24.69147246554616
23	29.73133221527255	18.736175531555883	22.06596615573624	29.466526097435324
24	31.579549910763028	19.63602080857495	21.63772267593011	27.146706604731907
25	28.537444226315	18.899854534241143	26.868560713593943	25.694140525849914
26	30.22129186861062	18.905278480843258	23.647290263563576	27.22613938698254
27	29.20195051917669	19.596182391445666	25.047016167999804	26.154850921377843
28	29.851381539234307	17.804385852826744	24.754204540948287	27.590028066990662
29	33.70060797101579	18.969486432523844	24.691836151541676	22.63806944491869
30	27.494796601055675	20.47606070693067	23.000989468832646	29.028153223181004
31	27.84098395653182	18.648023891889657	30.59998301306846	22.911009138510064
32	29.44381281172458	18.230580060138813	23.315961128308118	29.009645999828493
33	27.65848173557217	20.36211165109242	23.802200122815645	28.17720649051976
34	24.722510736530904	21.758330492119875	27.310208879191293	26.208949892157925
35	27.370502193254076	22.20989336368289	23.698408284120468	26.72119615894256
36	26.528616688330864	20.240460830055536	25.880005238838947	27.350917242774653
37	25.653039632213808	20.79787547393002	23.940505729575353	29.60857916428082
38	29.29374926970818	22.99590185067182	22.071772252080414	25.63857662753958
39	27.542674759909946	23.775516989161513	21.264806740569757	27.41700151035878
40	27.38222540973633	23.69210522423064	20.237088062711955	28.688581303321076
41	26.59720149665517	23.35750347629736	21.81099498395961	28.234300043087856
42	29.14769297829763	21.61026388951309	23.950992430821245	25.29105070136804
43	24.285448223274027	23.692783690121672	24.09983832876012	27.921929757844175
44	26.077655895103803	24.031144130056816	22.15338543023186	27.73781454460752
45	26.486185576192728	21.543029202407844	27.711533751135015	24.259251470264413
46	26.736619456689127	23.029694150450094	23.896430989611943	26.33725540324884
47	31.991247954779066	19.767069870392625	20.423778423929985	27.81790375089832
48	24.512939932787507	24.527306616662027	22.875513220297552	28.08424023025291
49	27.715958221117226	23.565170749150724	20.609071237809918	28.10979979192213
50	26.76916694906342	24.449180270399758	23.121720789464458	25.659931991072366
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1032.0
1	981.0
2	1324.5
3	1651.5
4	1508.5
5	1064.0
6	614.0
7	559.5
8	618.5
9	665.5
10	707.5
11	720.0
12	690.0
13	693.5
14	708.0
15	830.0
16	991.5
17	1230.5
18	1471.5
19	1913.5
20	2397.5
21	3026.0
22	3711.0
23	4482.0
24	5238.0
25	5873.5
26	6986.5
27	8795.0
28	10288.0
29	11632.0
30	13466.0
31	16070.5
32	18987.5
33	21748.0
34	24876.5
35	27905.5
36	32366.0
37	38246.0
38	47718.0
39	64591.5
40	80932.0
41	91561.5
42	109586.0
43	132257.0
44	128365.5
45	109971.5
46	105482.0
47	102384.5
48	103779.0
49	118368.0
50	142840.5
51	163428.5
52	173972.0
53	182072.5
54	181730.5
55	172820.0
56	166075.5
57	172815.0
58	193080.5
59	194370.0
60	171098.0
61	153934.5
62	147597.5
63	143910.5
64	152170.0
65	164393.0
66	144873.5
67	115725.0
68	98318.0
69	86161.5
70	77497.0
71	72621.0
72	72982.0
73	64775.5
74	49822.0
75	41914.0
76	38866.5
77	29474.0
78	21182.0
79	18871.0
80	16513.5
81	11417.0
82	7453.5
83	6566.0
84	5631.0
85	4381.0
86	3175.0
87	1862.5
88	871.0
89	588.0
90	422.5
91	293.0
92	191.5
93	151.5
94	94.5
95	30.0
96	16.5
97	15.0
98	18.5
99	22.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	12575.0
25	11940.0
26	15107.0
27	31766.0
28	43806.0
29	38732.0
30	8887.0
31	9163.0
32	9648.0
33	9431.0
34	10388.0
35	9272.0
36	8358.0
37	8004.0
38	8421.0
39	9783.0
40	11563.0
41	14801.0
42	18237.0
43	16150.0
44	14031.0
45	18211.0
46	36515.0
47	100742.0
48	444805.0
49	736006.0
50	684616.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.50911542142422
#Duplication Level	Percentage of deduplicated	Percentage of total
1	46.624840523407855	6.298603521362139
2	10.3175367137288	2.7876158866108858
3	6.5795624212405865	2.666522045130135
4	5.356985737007317	2.8947255452862053
5	4.495877430870749	3.036766356710457
6	3.6343902872294587	2.9458438726011527
7	3.0855504383878833	2.917813990756561
8	2.5678940375111146	2.775198155417976
9	2.216266649060015	2.6945821770124345
>10	14.077715751138772	32.847598145426424
>50	0.5990781159113078	5.555769832763341
>100	0.36558176730300906	9.820593748473204
>500	0.037358704435423336	3.5978494161792245
>1k	0.03302242624202599	8.598897955897113
>5k	0.00600407749855018	5.972022679538413
>10k+	0.0023349190272139585	4.58959667083441
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	25464	1.0877597974846196	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	14667	0.6265383659168596	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	13761	0.5878362619064502	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	13267	0.5667337901833351	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	12479	0.53307235755618	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	11552	0.4934731849097677	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC	10663	0.4554972793189797	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	9929	0.42414259461297465	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	9456	0.4039371915258625	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	9300	0.3972732530869841	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	9224	0.39402671897573555	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	8912	0.3806988420979787	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	8757	0.37407762121319565	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	8613	0.36792629342346167	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	7969	0.34041618858604045	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	6515	0.2783048649313657	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA	6507	0.2779631244986027	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	6452	0.2756136590233571	Illumina Single End Adapter 1 (100% over 32bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG	6404	0.2735632164267791	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	6129	0.2618158890505511	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG	5919	0.2528452026905224	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	5885	0.2513928058512797	Illumina Single End Adapter 1 (100% over 32bp)
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	5815	0.24840257706460347	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	5583	0.23849210451447656	No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC	5163	0.2205507317944192	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC	4689	0.20030261115321163	No Hit
CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGA	4625	0.19756868769110766	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	4493	0.1919299705505182	Illumina Single End Adapter 1 (100% over 31bp)
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	4158	0.17761958992856772	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	3958	0.16907607910949277	No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCT	3829	0.16356551463118946	No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC	3801	0.16236942311651897	No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCTG	3584	0.15309971387782267	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	3489	0.14904154623876206	No Hit
AGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCCGAG	3415	0.14588044723570437	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	3245	0.13861846303949069	Illumina Single End Adapter 1 (100% over 31bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	3111	0.13289431079071046	No Hit
CTGTGCGTCTGCTCCTCCTTCTCGCGCTTCTCCTGCCGCCCTAATCCT	2995	0.127939074515647	No Hit
TCGCTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAAT	2976	0.12712744098783488	No Hit
ATTAGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATA	2787	0.1190538232638091	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	2762	0.11798588441142473	Illumina Single End Adapter 1 (100% over 32bp)
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGATG	2755	0.11768686153275709	No Hit
CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGAA	2709	0.11572185404436987	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC	2583	0.11033944222835267	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	2516	0.10747736610396257	No Hit
AGCTGTTTTTCGTCTTCCCTAGGCTATTTCTGCCGGGCGCTCCGCGAAG	2498	0.10670845013024581	No Hit
ACCAGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCC	2441	0.10427354954680947	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC	2353	0.1005144047864165	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.19568911531091118	0.0	0.0	0.0	0.0
2	0.3080362825817464	0.0	0.0	0.0	0.0
3	0.374462079200054	0.0	0.0	0.0	0.0
4	0.460324362931757	0.0	0.0	0.0	0.0
5	0.564427042262185	0.0	0.0	0.0	0.0
6	0.68416434639152	0.0	0.0	0.0	0.0
7	0.7644733480908243	0.0	0.0	0.0	0.0
8	0.8485414945505216	0.0	0.0	0.0	0.0
9	0.9103537953265287	0.0	0.0	0.0	0.0
10	0.974814584456449	0.0	0.0	0.0	0.0
11	1.0300910994558639	0.0	0.0	0.0	0.0
12	1.0750726839182934	0.0	0.0	0.0	0.0
13	1.1191999172988152	0.0	0.0	0.0	0.0
14	1.164437807085817	4.271755409537463E-5	0.0	0.0	0.0
15	1.205489376571472	4.271755409537463E-5	0.0	0.0	0.0
16	1.2435507172704507	4.271755409537463E-5	0.0	0.0	0.0
17	1.2881478437460219	4.271755409537463E-5	0.0	0.0	0.0
18	1.3148035975015357	4.271755409537463E-5	0.0	0.0	0.0
19	1.3520533046727023	4.271755409537463E-5	0.0	0.0	0.0
20	1.3751635014383001	4.271755409537463E-5	0.0	0.0	0.0
21	1.4041687206690594	4.271755409537463E-5	0.0	0.0	0.0
22	1.4232634673496918	4.271755409537463E-5	0.0	0.0	0.0
23	1.444237786410521	4.271755409537463E-5	0.0	0.0	0.0
24	1.4657247161204943	4.271755409537463E-5	0.0	0.0	0.0
25	1.4845204399224592	4.271755409537463E-5	0.0	0.0	0.0
26	1.5103645601501607	4.271755409537463E-5	0.0	0.0	0.0
27	1.5263409253818307	4.271755409537463E-5	0.0	0.0	0.0
28	1.5441114278855066	4.271755409537463E-5	0.0	0.0	0.0
29	1.5645304187430957	4.271755409537463E-5	0.0	0.0	0.0
30	1.5790116695814278	4.271755409537463E-5	0.0	0.0	0.0
31	1.593621073082046	4.271755409537463E-5	0.0	0.0	0.0
32	1.6059237286615138	4.271755409537463E-5	0.0	0.0	0.0
33	1.6186535597819354	4.271755409537463E-5	0.0	0.0	0.0
34	1.6323658946465507	4.271755409537463E-5	0.0	0.0	0.0
35	1.6461209470652614	4.271755409537463E-5	0.0	0.0	0.0
36	1.6591070835102553	4.271755409537463E-5	0.0	0.0	0.0
37	1.670042777358671	4.271755409537463E-5	0.0	0.0	0.0
38	1.6804231430038472	4.271755409537463E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGTC	1260	0.0	123.73618	44
TAAGCCA	1870	0.0	96.772285	44
CTATACG	130	0.0	85.66352	44
GGTCCCA	1470	0.0	76.23034	44
AATGAAG	1780	0.0	71.94772	44
CCAGAAT	1345	0.0	68.82538	44
TATTAGT	1275	0.0	59.626003	43
CGCTACC	330	0.0	56.94677	44
CAATGAA	4205	0.0	56.317596	43
CGACGGG	3070	0.0	54.865112	44
AGGTCCC	2680	0.0	53.47026	43
TCGTGCG	1065	0.0	51.16852	43
ATAAGAT	1585	0.0	50.51091	43
TTAAGCC	1770	0.0	50.17278	43
AATCGCA	70	2.1582206E-5	49.715435	44
AATCCTG	1630	0.0	49.529175	43
GACCTTA	340	0.0	47.48974	43
TAAGATG	1310	0.0	46.755276	44
AATCCCG	1375	0.0	46.23774	43
TTTGGAC	1620	0.0	45.88965	43
>>END_MODULE