FastQCFastQC Report
Tue 24 May 2016
ERR841145.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841145.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4025528
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA932862.3173606046213067No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA322530.8012116671403104No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC254700.6327120318129696No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG216190.5370475624564032No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC201470.5004809307002709No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC192960.4793408467162569No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC190430.4730559568831716No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG173350.4306267401443985No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT165230.41045547317022757No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG145840.3622878787577679No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA134810.3348877464024595No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA133280.3310870027484593No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC131310.32619323477566176No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA128520.31926246693601434Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA124560.30942524806683747Illumina Single End Adapter 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC114750.2850557740500128No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA113880.2828945668742088Illumina Single End Adapter 1 (100% over 31bp)
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG108370.26920692142745No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG85810.2131645836272906No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG85630.21271743731505532No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC83140.20653191332913345No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT82820.20573698655182623No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA81200.20171266974170843Illumina Single End Adapter 1 (100% over 31bp)
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT80950.20109163319693715No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC80470.19989924303097634No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA77400.19227291426118512No Hit
CTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAATCCT74920.18611223173705413No Hit
CTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAATCCTG72670.1805229028341127No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT72640.18044837844874015No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC71820.17841137858189038No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA71230.17694573233623018No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT65730.16328292835126224No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG62970.15642668489698744No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC61120.15183101446568004No Hit
TCGCTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAAT60770.15096156330300026No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG58610.1455958075561765No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA58000.14408047838693458Illumina Single End Adapter 1 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGTCGGGGAAGCAGTGGT55110.1369012959293787No Hit
CTGTGCGTCTGCTCCTCCTTCTCGCGCTTCTCCTGCCGCCCTAATCCT53140.1320075279565811No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG50100.12445572357216246No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA48570.12065497991816229Illumina Single End Adapter 1 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTG47650.118369565433404No Hit
AGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCCGAG47440.11784789473579615No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA43560.10820940756094603Illumina Single End Adapter 1 (100% over 32bp)
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC43220.1073647978600571No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCAT41990.10430929805978248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA111000.0111.1228944
ATTAGTC14000.0108.2299844
CTATACG4100.099.2635444
CGACGGG38650.064.6373744
TTAAGCC108800.058.31627343
AATGAAG24450.058.13092844
CTATGCG1550.056.55312344
CAATGAA55400.053.84750443
CCAGAAT20800.052.6786944
AATCCCG28350.052.61290743
GGTCCCA19050.051.93048544
TATTAGT15150.051.45007743
AATCCTG36050.050.71798343
AGGTCCC37150.050.0796843
TTTGGAC29200.049.21387543
ATCCTTG15800.047.10025843
ATTAAGC108900.047.0367542
TAATCCT69750.046.06542
TAATCCC58950.044.6742
CTAATCC127400.044.1399941
Produced by FastQC (version 0.11.5)