##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841145.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4025528
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.17387433400041	38.0	35.0	39.0	31.0	39.0
2	37.055628479046725	39.0	35.0	39.0	33.0	39.0
3	37.210951209381726	39.0	35.0	39.0	35.0	39.0
4	37.5870188954095	39.0	35.0	39.0	34.0	41.0
5	38.215356842630335	40.0	35.0	41.0	35.0	41.0
6	38.18702043558013	40.0	35.0	41.0	35.0	41.0
7	38.12308000341819	40.0	35.0	41.0	34.0	41.0
8	38.09760856215632	40.0	35.0	41.0	34.0	41.0
9	38.115692401096204	40.0	35.0	41.0	34.0	41.0
10	38.015946479567404	40.0	35.0	41.0	34.0	41.0
11	38.07559629444883	40.0	35.0	41.0	34.0	41.0
12	38.045481735563634	40.0	35.0	41.0	34.0	41.0
13	38.003633063786914	40.0	35.0	41.0	34.0	41.0
14	37.96406036673947	40.0	35.0	41.0	34.0	41.0
15	37.91943864258303	39.0	35.0	41.0	34.0	41.0
16	37.84338377474955	39.0	35.0	41.0	34.0	41.0
17	37.74929475089976	39.0	35.0	41.0	34.0	41.0
18	37.688494776337414	39.0	35.0	41.0	34.0	41.0
19	37.55260502473216	39.0	35.0	41.0	34.0	41.0
20	37.46641956036574	39.0	35.0	40.0	33.0	41.0
21	37.08728445063604	38.0	35.0	40.0	33.0	41.0
22	36.95154151206997	38.0	35.0	40.0	33.0	41.0
23	36.81149628073634	38.0	35.0	40.0	33.0	41.0
24	36.68401039565493	37.0	35.0	40.0	33.0	41.0
25	36.582018312853116	37.0	35.0	40.0	33.0	41.0
26	36.42898069141621	37.0	35.0	40.0	32.0	41.0
27	36.36363847999306	36.0	35.0	40.0	32.0	41.0
28	36.43909786665073	36.0	35.0	40.0	33.0	41.0
29	36.46582300771787	36.0	35.0	40.0	33.0	41.0
30	36.45747038440381	36.0	35.0	40.0	33.0	41.0
31	36.39622085528012	36.0	35.0	40.0	33.0	41.0
32	36.328323568560485	35.0	35.0	39.0	33.0	41.0
33	36.26598084590898	35.0	35.0	39.0	33.0	41.0
34	36.19796228819215	35.0	35.0	39.0	33.0	41.0
35	36.041001243343935	35.0	35.0	39.0	33.0	41.0
36	35.943467831757935	35.0	35.0	39.0	33.0	41.0
37	35.911266546125354	35.0	35.0	39.0	33.0	41.0
38	35.81412802044299	35.0	35.0	38.0	33.0	41.0
39	35.670212521892886	35.0	35.0	37.0	33.0	41.0
40	35.52148425796807	35.0	35.0	37.0	33.0	41.0
41	35.37957923837882	35.0	35.0	37.0	32.0	41.0
42	35.31015704023727	35.0	35.0	37.0	33.0	41.0
43	35.28942208566635	35.0	35.0	36.0	33.0	41.0
44	35.25208503393428	35.0	35.0	36.0	33.0	41.0
45	35.19707710463277	35.0	35.0	36.0	33.0	40.0
46	35.10989579332922	35.0	35.0	36.0	33.0	40.0
47	35.05884958073333	35.0	35.0	36.0	33.0	40.0
48	35.000409357683445	35.0	35.0	36.0	33.0	39.0
49	35.053848728259446	35.0	34.0	36.0	32.0	40.0
50	35.651772421317105	35.0	35.0	39.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	41.0
14	192.0
15	573.0
16	82.0
17	0.0
18	0.0
19	2.0
20	5.0
21	25.0
22	105.0
23	270.0
24	713.0
25	1705.0
26	3762.0
27	7728.0
28	14781.0
29	26678.0
30	45670.0
31	75299.0
32	124646.0
33	233748.0
34	646213.0
35	310978.0
36	467102.0
37	607011.0
38	603333.0
39	486681.0
40	368185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	46.97681894151525	19.883851261250697	33.139329797234055
2	29.550732226927746	22.05042419280154	27.25719458416387	21.141648996106845
3	33.61718015624286	14.243994825026679	22.169489319165088	29.969335699565374
4	23.223040555176862	16.696194884248726	27.010841807583997	33.069922752990415
5	27.064399999205076	20.51306064695116	28.556676291905063	23.865863061938708
6	29.902238911268284	23.044157188820943	21.743955078687815	25.309648821222957
7	35.20348138182122	20.40480155646663	21.686124155638716	22.705592906073438
8	30.77397548843282	17.573346900083667	26.93052936161418	24.72214824986933
9	27.08183870538225	20.165603120882526	25.40993380247262	27.342624371262602
10	31.586340971917227	18.985733051664276	23.984605249298973	25.443320727119524
11	31.590216239956597	21.008697492602213	24.35792273709188	23.04316353034931
12	27.81202863326252	16.224082902913604	26.750677178248417	29.213211285575458
13	25.074946690222998	16.776308598524217	28.192078157200744	29.956666554052042
14	25.516081368704924	20.190966253370988	21.36924646903462	32.92370590888947
15	23.697661524152856	21.24747362333587	26.010650031498972	29.0442148210123
16	29.03132210234285	21.408520820125958	25.800218008668672	23.759939068862522
17	29.844482512604557	19.138657090448756	19.825150887038916	31.191709509907767
18	27.829144400436416	19.287333239267	19.578922317768004	33.304600042528584
19	27.12528642205445	21.310869033826123	26.344171497502938	25.219673046616492
20	33.704994723673515	17.07877823728962	22.405011218404145	26.81121582063272
21	28.04702886180397	19.5387536740522	24.367213443801656	28.047004020342175
22	28.69486934384756	21.026806918247743	26.12141810962438	24.156905628280313
23	30.073346900083664	18.375974530546056	22.89192871096661	28.65874985840367
24	31.052398592184677	19.801129193487167	21.458178902245866	27.68829331208229
25	26.811846493952157	20.697173092327823	25.772335269925257	26.71864514379476
26	29.571676153731918	18.982246837582096	25.35635859523589	26.089718413450097
27	28.749541676503696	21.261604852143385	24.979180916130787	25.009672555222128
28	28.79681604823336	17.430950714062345	26.872520073503438	26.89971316420086
29	33.79159639378251	18.358439402150275	25.178324540706214	22.671639663361002
30	26.332430801023076	19.61156758218152	23.176226683280756	30.879774933514643
31	26.775192752189657	18.511334987537012	32.213577787677785	22.499894472595543
32	28.56859965674034	18.49220236488777	25.130140688912896	27.809057289458988
33	28.490542463848417	20.883445780776377	22.9144801886075	27.711531566767704
34	24.22770334877294	21.31053704892097	30.010118680908338	24.45164092139775
35	27.365236674692017	22.025777419468216	22.435273623534787	28.173712282304976
36	25.84340700887175	21.206848590752493	26.123346826226474	26.82639757414928
37	24.592895789252157	21.478277192709072	23.34013307605776	30.58869394198101
38	29.455501517183606	24.896598701593824	20.967377210610035	24.68052257061253
39	26.363318430314862	26.20664088948078	21.10527611618759	26.324764564016768
40	26.96545071495103	25.964865295962912	19.830062516896554	27.2396214721895
41	27.883422404464593	23.302520225438556	21.12881835068264	27.685239019414208
42	27.678598981638636	24.28240383571351	23.75950784915451	24.279489333493345
43	24.028362937016492	24.80157206097656	25.2616106983312	25.90845430367575
44	25.262903188189473	24.28825477528745	24.476059425799377	25.972782610723698
45	25.757405679117408	23.784947917523738	27.150914587559917	23.30673181579894
46	26.42304426774344	24.59839699838288	22.75367291519192	26.22488581868176
47	32.03258263083731	19.71340212277305	20.75238876786187	27.50162647852777
48	24.071712911374537	23.258701450619927	22.57353942186972	30.096046216135814
49	27.861685978740176	22.32273768602739	19.94659344910507	29.86898288612736
50	25.02459677357656	29.223415210369357	22.123172073366476	23.628815942687606
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9134.0
1	8943.0
2	6673.5
3	4426.5
4	4094.5
5	2975.0
6	1666.0
7	1390.0
8	1517.0
9	1531.5
10	1509.0
11	1496.5
12	1459.0
13	1481.0
14	1550.5
15	1842.0
16	2182.5
17	2680.0
18	3195.5
19	4199.0
20	5249.0
21	6433.5
22	7861.0
23	9954.5
24	12141.0
25	14036.0
26	16087.5
27	18257.0
28	20549.5
29	22963.0
30	25621.0
31	29272.5
32	33606.0
33	38403.0
34	43081.0
35	47751.0
36	55365.5
37	64677.5
38	84004.0
39	117950.0
40	149820.5
41	173888.0
42	202518.5
43	265750.5
44	291052.5
45	226852.0
46	184347.5
47	179079.5
48	188086.0
49	217596.0
50	254345.0
51	287064.5
52	303440.5
53	308633.0
54	300364.5
55	280797.0
56	265555.5
57	276510.5
58	314026.5
59	320823.0
60	281643.0
61	252695.5
62	242204.5
63	233102.0
64	248913.5
65	272850.5
66	241293.5
67	192346.5
68	161315.0
69	140590.5
70	126356.5
71	116846.5
72	114845.5
73	100494.5
74	78052.5
75	66950.5
76	62776.0
77	48448.0
78	36062.5
79	33378.5
80	30184.0
81	21115.5
82	13605.5
83	11913.0
84	10254.5
85	8142.0
86	6080.5
87	3599.5
88	1669.5
89	1150.5
90	796.5
91	518.0
92	378.0
93	323.5
94	216.5
95	84.5
96	37.5
97	32.0
98	24.0
99	16.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	24513.0
25	21386.0
26	27726.0
27	53854.0
28	81973.0
29	80291.0
30	16372.0
31	17842.0
32	19851.0
33	18199.0
34	21729.0
35	18156.0
36	17127.0
37	15645.0
38	16644.0
39	19501.0
40	23646.0
41	31333.0
42	36770.0
43	32810.0
44	27096.0
45	31015.0
46	55067.0
47	143718.0
48	624513.0
49	1241669.0
50	1307082.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.280505329809769
#Duplication Level	Percentage of deduplicated	Percentage of total
1	46.27519186033411	6.145579321391369
2	10.553416176862507	2.8030939956904635
3	6.72712184480726	2.680187325427277
4	5.642822796024402	2.9975815287109655
5	4.531596020373466	3.009094255055728
6	3.8236401671270515	3.0467924171283314
7	3.1864836825043112	2.9622679470195257
8	2.6765608956605202	2.8436864992303956
9	2.1645395360370228	2.5871560960431284
>10	13.427864643440856	30.34321847514949
>50	0.5470078740648411	4.99007906087388
>100	0.36477987175954196	9.637078460933878
>500	0.03840412230880601	3.5728247309716554
>1k	0.03269274001672549	8.437271072982249
>5k	0.004332772773300969	4.044245825626983
>10k+	0.0035449959054280646	9.899842987764691
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	93286	2.3173606046213067	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	32253	0.8012116671403104	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	25470	0.6327120318129696	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	21619	0.5370475624564032	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	20147	0.5004809307002709	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	19296	0.4793408467162569	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	19043	0.4730559568831716	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	17335	0.4306267401443985	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	16523	0.41045547317022757	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG	14584	0.3622878787577679	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	13481	0.3348877464024595	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	13328	0.3310870027484593	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	13131	0.32619323477566176	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	12852	0.31926246693601434	Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	12456	0.30942524806683747	Illumina Single End Adapter 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC	11475	0.2850557740500128	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	11388	0.2828945668742088	Illumina Single End Adapter 1 (100% over 31bp)
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	10837	0.26920692142745	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG	8581	0.2131645836272906	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	8563	0.21271743731505532	No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC	8314	0.20653191332913345	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	8282	0.20573698655182623	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	8120	0.20171266974170843	Illumina Single End Adapter 1 (100% over 31bp)
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	8095	0.20109163319693715	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	8047	0.19989924303097634	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	7740	0.19227291426118512	No Hit
CTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAATCCT	7492	0.18611223173705413	No Hit
CTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAATCCTG	7267	0.1805229028341127	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	7264	0.18044837844874015	No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC	7182	0.17841137858189038	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA	7123	0.17694573233623018	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	6573	0.16328292835126224	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	6297	0.15642668489698744	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC	6112	0.15183101446568004	No Hit
TCGCTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAAT	6077	0.15096156330300026	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	5861	0.1455958075561765	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	5800	0.14408047838693458	Illumina Single End Adapter 1 (100% over 32bp)
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAGTCGGGGAAGCAGTGGT	5511	0.1369012959293787	No Hit
CTGTGCGTCTGCTCCTCCTTCTCGCGCTTCTCCTGCCGCCCTAATCCT	5314	0.1320075279565811	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG	5010	0.12445572357216246	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	4857	0.12065497991816229	Illumina Single End Adapter 1 (100% over 31bp)
AAGCAGTGGTATCAACGCAGAGTG	4765	0.118369565433404	No Hit
AGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCCGAG	4744	0.11784789473579615	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	4356	0.10820940756094603	Illumina Single End Adapter 1 (100% over 32bp)
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC	4322	0.1073647978600571	No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCAT	4199	0.10430929805978248	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.29285599305233995	0.0	0.0	0.0	0.0
2	0.4462023366872619	0.0	0.0	0.0	0.0
3	0.5379915380044555	0.0	0.0	0.0	0.0
4	0.6535540182554934	0.0	0.0	0.0	0.0
5	0.8040435937844675	0.0	0.0	0.0	0.0
6	0.9462112796135066	0.0	0.0	0.0	0.0
7	1.0394412857145696	0.0	0.0	0.0	0.0
8	1.1439741569304698	0.0	0.0	0.0	0.0
9	1.218672432535558	0.0	0.0	0.0	0.0
10	1.2893463913305285	0.0	0.0	0.0	0.0
11	1.3707518616191465	0.0	0.0	0.0	0.0
12	1.4230182972270966	0.0	0.0	0.0	0.0
13	1.4817683543624587	0.0	0.0	0.0	0.0
14	1.5422821552849713	0.0	0.0	0.0	0.0
15	1.6032182610579282	0.0	0.0	0.0	0.0
16	1.6519075261679959	0.0	0.0	0.0	0.0
17	1.7321702892142348	0.0	0.0	0.0	2.484146179085079E-5
18	1.774897603494498	0.0	0.0	0.0	2.484146179085079E-5
19	1.84999334248824	0.0	0.0	0.0	2.484146179085079E-5
20	1.881790413580529	0.0	0.0	0.0	2.484146179085079E-5
21	1.9254368619470539	0.0	0.0	0.0	2.484146179085079E-5
22	1.9489368848011988	0.0	0.0	0.0	2.484146179085079E-5
23	1.9784236999469387	0.0	0.0	0.0	2.484146179085079E-5
24	2.007289478547907	0.0	0.0	0.0	2.484146179085079E-5
25	2.0335717451226274	0.0	0.0	0.0	2.484146179085079E-5
26	2.0834782418604467	0.0	0.0	0.0	2.484146179085079E-5
27	2.1046183258444606	0.0	0.0	0.0	2.484146179085079E-5
28	2.1264042878350367	0.0	0.0	0.0	2.484146179085079E-5
29	2.152140042250358	0.0	0.0	0.0	2.484146179085079E-5
30	2.169504224042163	0.0	0.0	0.0	2.484146179085079E-5
31	2.1845084669638366	0.0	0.0	0.0	2.484146179085079E-5
32	2.1972272954007526	0.0	0.0	0.0	2.484146179085079E-5
33	2.2115359773922822	0.0	0.0	0.0	2.484146179085079E-5
34	2.226465695928584	0.0	0.0	0.0	2.484146179085079E-5
35	2.2406998535347413	0.0	0.0	0.0	2.484146179085079E-5
36	2.2531454258919577	0.0	0.0	0.0	2.484146179085079E-5
37	2.266236876255736	0.0	0.0	0.0	2.484146179085079E-5
38	2.2761237780484946	0.0	0.0	0.0	2.484146179085079E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	11100	0.0	111.12289	44
ATTAGTC	1400	0.0	108.22998	44
CTATACG	410	0.0	99.26354	44
CGACGGG	3865	0.0	64.63737	44
TTAAGCC	10880	0.0	58.316273	43
AATGAAG	2445	0.0	58.130928	44
CTATGCG	155	0.0	56.553123	44
CAATGAA	5540	0.0	53.847504	43
CCAGAAT	2080	0.0	52.67869	44
AATCCCG	2835	0.0	52.612907	43
GGTCCCA	1905	0.0	51.930485	44
TATTAGT	1515	0.0	51.450077	43
AATCCTG	3605	0.0	50.717983	43
AGGTCCC	3715	0.0	50.07968	43
TTTGGAC	2920	0.0	49.213875	43
ATCCTTG	1580	0.0	47.100258	43
ATTAAGC	10890	0.0	47.03675	42
TAATCCT	6975	0.0	46.065	42
TAATCCC	5895	0.0	44.67	42
CTAATCC	12740	0.0	44.13999	41
>>END_MODULE