FastQCFastQC Report
Tue 24 May 2016
ERR841144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1956787
Sequences flagged as poor quality0
Sequence length24-50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC227521.1627223606861656No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC207651.0611783500197007No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA195080.996940392592551No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG195070.9968892884100313No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT177650.9078658024608708No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC172750.882824753026262No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC147740.7550131925447174No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA102660.5246355377463158No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC99460.5082821993400406No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA92720.4738379803218235No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT91920.4697496457202546No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA85530.4370940730902239No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG82960.42396029818268416No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT82950.4239091940001645No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA77800.39759054000256544No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC77740.39728391490744774No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG69440.3548674434161715No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT56640.2894540897910708No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT56190.2871544015776883No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA54160.27678025252620747Illumina Single End Adapter 1 (100% over 32bp)
TCGCTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAAT52630.2689613126007072No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA51430.262828810698354Illumina Single End Adapter 1 (100% over 32bp)
CTGTGCGTCTGCTCCTCCTTCTCGCGCTTCTCCTGCCGCCCTAATCCT50980.2605291224849715No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT46150.23584580232799993No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC44770.22879342514029377No Hit
CTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAATCCT44580.22782244567242116No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG39290.2007883331195475No Hit
CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGA37810.19322491410664525No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC37450.19138516353593926No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG37210.19015866315546864No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG34380.175696179502419No Hit
ATTAGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATA30160.15413021447914363No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC29840.1524948806385161No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC28900.14769108748167276No Hit
TTGTCTTTCTAGGTCTCAGCCGGTCGTCGCGACGTTCGCCCGCTCGCT27930.1427339817772706No Hit
ATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAG26860.13726583424767233No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC26000.13287087455098587No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG25700.13133774907539758No Hit
AGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGT25700.13133774907539758No Hit
TCTGGAAGAGGTCTTGCGAAAGCCGCCTCGAGCGCGCTCCGCGGCTGC25220.12888474831445632No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA24780.12663616428359345No Hit
TCGTTGTCTTTCTAGGTCTCAGCCGGTCGTCGCGACGTTCGCCCGCTC24770.12658506010107387No Hit
TCGCTGTGCGTCTGCTCCTCCTTCTCGCGCTTCTCCTGCCGCCCTAAT23430.11973709964344612No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG23430.11973709964344612No Hit
ACACCCGGGTGAGGGGTCTGCTGGCTGCACCTGTCGGTCTCATGGCTCC23120.11815286998533821No Hit
CTCTTTTTAAGTTAGTGCTGGAACGTGGAAGAGCTGCTGCCTCCGAAG22750.11626201523211264No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA22300.1139623270187302Illumina Single End Adapter 1 (100% over 32bp)
CGGCTCCTGGCCAGGTAACTTACTACTTCCAGTCAATATGCCGACCTT21970.11227588899558305No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCT21140.10803424184645544No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA21110.1078809292988966Illumina Single End Adapter 1 (100% over 31bp)
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT20350.10399701142740625No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT20100.102719406864416No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG19710.1007263437461512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA16150.0158.1267544
CGACGGG32300.0140.4845144
ATTAGTC3950.0138.9212844
AATCGCA451.8556479E-593.8015644
TATATTA1750.084.42139444
ACTACAC1750.078.391344
GGCGGGC31800.069.0237844
CGGGCGC26600.067.0451944
CAATGAA24350.065.2229643
CGTTAAT251.2975509E-463.36873643
AGGTCCC20000.062.77466243
TTAAGCC15700.061.80470343
GACGGGC11600.060.04107744
TCGTGCG10250.059.50479543
ATAAGAT8750.057.93713443
ACAACGT1551.2532837E-954.4654244
GGTCGCT9450.053.60088744
TATTAGT3700.053.52089743
AGGTCGA601.6534614E-952.80728543
CCGACGG35450.049.26941743
Produced by FastQC (version 0.11.5)