##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR841144.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1956787 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 56 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.12468296242769 39.0 37.0 39.0 33.0 39.0 2 37.80697899158161 39.0 37.0 39.0 35.0 39.0 3 38.312094775772735 39.0 38.0 39.0 35.0 41.0 4 38.68887058223506 39.0 38.0 41.0 35.0 41.0 5 39.076672116076 40.0 38.0 41.0 35.0 41.0 6 39.0950563346956 40.0 39.0 41.0 35.0 41.0 7 39.02445590654476 40.0 38.0 41.0 35.0 41.0 8 39.026424439655415 40.0 38.0 41.0 35.0 41.0 9 38.97459866607863 40.0 38.0 41.0 35.0 41.0 10 38.96894245515736 40.0 38.0 41.0 35.0 41.0 11 38.97278344551553 40.0 38.0 41.0 35.0 41.0 12 38.90178798203382 40.0 38.0 41.0 35.0 41.0 13 38.87499610330608 40.0 38.0 41.0 35.0 41.0 14 38.89020879635852 40.0 38.0 41.0 35.0 41.0 15 38.86719351671899 40.0 38.0 41.0 35.0 41.0 16 38.817599462792835 40.0 38.0 41.0 35.0 41.0 17 38.7242239446603 40.0 38.0 41.0 35.0 41.0 18 38.643436919807826 40.0 38.0 41.0 35.0 41.0 19 38.514919610565684 40.0 38.0 41.0 35.0 41.0 20 38.49269337950426 40.0 37.0 41.0 35.0 41.0 21 38.235521290769 40.0 37.0 41.0 34.0 41.0 22 38.09300859010204 40.0 36.0 41.0 33.0 41.0 23 38.002916004654566 40.0 36.0 41.0 33.0 41.0 24 37.844366811512955 39.0 36.0 41.0 33.0 41.0 25 37.809572352153026 39.0 35.0 41.0 33.0 41.0 26 37.736963693133944 39.0 35.0 41.0 33.0 41.0 27 37.721086485004115 39.0 35.0 41.0 33.0 41.0 28 37.85971963595182 39.0 35.0 41.0 34.0 41.0 29 37.89086778624242 39.0 35.0 41.0 34.0 41.0 30 37.888211929943495 39.0 35.0 41.0 34.0 41.0 31 37.81053012549751 39.0 35.0 41.0 34.0 41.0 32 37.7658845837232 39.0 35.0 41.0 34.0 41.0 33 37.65592017482812 39.0 35.0 41.0 34.0 41.0 34 37.54684219931294 39.0 35.0 41.0 34.0 41.0 35 37.39541662703586 38.0 35.0 41.0 33.0 41.0 36 37.26477282178429 38.0 35.0 41.0 33.0 41.0 37 37.20620395860794 38.0 35.0 41.0 34.0 41.0 38 37.04445676350277 37.0 35.0 41.0 33.0 41.0 39 36.885383260117266 37.0 35.0 40.0 33.0 41.0 40 36.71258459507424 36.0 35.0 40.0 33.0 41.0 41 36.490719826881076 36.0 35.0 40.0 33.0 41.0 42 36.36429929405025 36.0 35.0 40.0 33.0 41.0 43 36.27144857867444 35.0 35.0 40.0 33.0 41.0 44 36.18599646543284 35.0 35.0 39.0 33.0 41.0 45 36.07331171318827 35.0 35.0 39.0 33.0 41.0 46 35.93431761203244 35.0 35.0 39.0 33.0 41.0 47 35.906462577762575 35.0 35.0 39.0 33.0 41.0 48 35.82486029712417 35.0 35.0 39.0 33.0 41.0 49 36.28506810454621 36.0 35.0 40.0 33.0 41.0 50 36.831440196321374 38.0 35.0 40.0 33.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 81.0 15 400.0 16 283.0 17 3.0 18 0.0 19 0.0 20 4.0 21 14.0 22 43.0 23 68.0 24 179.0 25 386.0 26 863.0 27 1737.0 28 3666.0 29 7045.0 30 12673.0 31 21886.0 32 36092.0 33 63399.0 34 140984.0 35 119300.0 36 172754.0 37 251958.0 38 374198.0 39 398312.0 40 350459.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 44.01475479957706 15.675952466977755 40.30929273344518 2 29.846784550387955 17.822430341166413 30.460085844805796 21.870699263639835 3 35.42715686479929 13.698987166206644 21.824501082642104 29.049354886351964 4 22.80968751325515 17.243266640671674 28.436360217029243 31.510685629043937 5 29.47459278909764 18.6047331671766 28.52461714024061 23.396056903485153 6 29.173333633144537 22.887519183232513 21.355313582929565 26.583833600693378 7 33.91723268807489 19.40149847683984 22.00474553438877 24.6765233006965 8 32.489432932659504 15.732984734669639 26.039880681954653 25.7377016507162 9 25.794427293312967 20.922103427710834 24.375928499116153 28.907540779860046 10 30.15034339455444 17.674994774597337 24.5897483987782 27.584913432070017 11 33.7251831701662 16.945380360764865 25.15444961561989 24.174986853449045 12 28.117265701376798 16.150505905854853 23.89059207772742 31.841636315040933 13 24.88702142849477 16.627001303667697 31.097201688277774 27.38877557955976 14 25.453409083359606 21.563563126696977 21.189327198105875 31.79370059183754 15 25.578818747262734 19.63964396738122 24.3802212504478 30.401316034908245 16 26.84737786994701 21.704048524443387 25.730598169345974 25.71797543626363 17 28.38029892880523 17.876651878819718 22.098623917677294 31.644425274697756 18 29.95553425078969 19.515256387128492 19.144546647131243 31.38466271495058 19 26.578007723886145 19.332507830438367 28.661167515933005 25.42831692974248 20 32.64090572964763 15.771312871559347 22.584420276708705 29.003361122084314 21 30.502604524662114 18.320185078907414 20.90426806801149 30.272942328418985 22 28.51414078282409 18.255487183837587 27.82781161158573 25.402560421752597 23 30.05058803027616 18.44738338919872 21.203534160846328 30.29849441967879 24 32.941756052140576 20.091098315759456 20.248601406284894 26.718544225815073 25 29.01476094540304 17.706715118324308 26.57042069439045 26.7081032418822 26 32.29608767601691 17.844756812987725 22.42814970228896 27.431005808706406 27 28.081397039880997 19.28341560502938 24.456560128975426 28.1786272261142 28 29.222405860638535 17.05066834161821 25.042481804464213 28.684443993279046 29 35.1333935827862 18.349247672703115 24.048213222669897 22.469145521840787 30 28.26020518343771 21.28057864681714 21.441587233030543 29.01762893671461 31 26.672682228408647 18.486374093615396 31.43105380888857 23.40988986908739 32 28.95055911771825 17.055337243288573 22.963056491814072 31.031047147179102 33 28.212200733178776 19.00358167141711 21.82930099781465 30.954916597589467 34 24.63808777857012 20.235854597155026 28.68888825798378 26.437169366291073 35 28.89802107053538 22.074323152411697 22.653063508137837 26.374592268915087 36 26.8743073834887 19.862724250400678 24.060851347918835 29.202117018191792 37 26.89677421902011 19.385706800406318 23.48131440718738 30.236204573386193 38 31.43524831144593 21.216381805744934 20.89361996095148 26.454749921857655 39 26.725561684847033 22.3832527264861 20.59559430091492 30.29559128775195 40 27.549672126279916 23.60011283149963 18.91606387085133 29.934151171369123 41 27.603169637604 21.205748053416603 20.865939023795242 30.325143285184154 42 29.626327120852313 19.903520970588506 24.550176487437973 25.919975421121205 43 25.174714002451616 23.669620057825515 22.518350720780788 28.637315218942078 44 27.742123430280397 22.98625700614291 21.384659931528773 27.88695963204792 45 27.92675756443559 19.881730661624992 28.946618926291567 23.244892847647847 46 27.84801645768541 22.769475755242777 22.419229506107925 26.96327828096389 47 32.305437043235536 18.38369894850644 20.02754711564597 29.28331689261206 48 25.113092804247227 21.744906767395523 23.458660280249486 29.68334014810776 49 30.594788722091565 22.046373018314526 20.085755713456873 27.273082546137033 50 28.896118602835575 24.90600927783684 20.183691577205618 26.014180542121967 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 354.0 1 538.5 2 1498.0 3 2229.0 4 2044.0 5 1316.5 6 623.0 7 577.0 8 695.5 9 759.5 10 748.5 11 702.0 12 635.5 13 599.5 14 597.5 15 687.0 16 800.0 17 973.5 18 1146.0 19 1407.0 20 1710.0 21 2182.5 22 2690.5 23 3203.0 24 3711.0 25 4422.5 26 5473.5 27 6549.5 28 7696.0 29 8674.5 30 9711.0 31 11281.0 32 13244.0 33 15523.0 34 17752.5 35 19944.0 36 24219.0 37 28989.5 38 35666.0 39 48202.0 40 59583.5 41 71056.0 42 89971.5 43 101770.5 44 92654.0 45 86463.5 46 90914.0 47 82957.5 48 79935.0 49 93328.0 50 112343.0 51 126350.0 52 132643.0 53 142705.0 54 143811.5 55 132499.0 56 128028.0 57 138633.0 58 156185.0 59 157891.5 60 142456.0 61 134394.5 62 133754.5 63 130655.5 64 144749.5 65 160331.0 66 130745.5 67 98394.0 68 86393.0 69 77319.0 70 70499.5 71 71150.5 72 76858.0 73 65022.5 74 47768.0 75 44092.5 76 43423.5 77 31368.5 78 21872.5 79 22135.0 80 21704.0 81 14663.5 82 8871.5 83 7857.5 84 6985.0 85 5674.0 86 4223.0 87 2335.0 88 1001.5 89 711.0 90 477.5 91 269.5 92 167.5 93 131.5 94 93.0 95 48.5 96 36.5 97 36.0 98 23.0 99 9.5 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 9554.0 25 9280.0 26 12625.0 27 33930.0 28 36886.0 29 25417.0 30 8262.0 31 7172.0 32 8917.0 33 8383.0 34 9031.0 35 7846.0 36 6487.0 37 6989.0 38 7059.0 39 7665.0 40 9129.0 41 10587.0 42 14333.0 43 14622.0 44 13756.0 45 17176.0 46 34486.0 47 96425.0 48 542819.0 49 622648.0 50 375303.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.477756796099131 #Duplication Level Percentage of deduplicated Percentage of total 1 51.00474338479896 6.874295267877661 2 9.732253299061606 2.6233788608557145 3 6.113442982535091 2.4718649311628056 4 4.836963792643423 2.6076568651514127 5 3.969582363504393 2.6750532838698287 6 3.275650726749653 2.648905430645831 7 2.7604195099757143 2.604298396746187 8 2.30915358861639 2.489776837776899 9 1.956803335012862 2.3735987502389135 >10 12.973137099851945 30.93808788144228 >50 0.6006453764764015 5.534936145677478 >100 0.3831634853107608 10.28139700311667 >500 0.04540606788040983 4.45918185408788 >1k 0.02947411423816077 7.950589135054107 >5k 0.005974482615843399 5.811317750903288 >10k+ 0.003186390728449813 7.655661605393005 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC 22752 1.1627223606861656 No Hit CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC 20765 1.0611783500197007 No Hit AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA 19508 0.996940392592551 No Hit CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG 19507 0.9968892884100313 No Hit CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT 17765 0.9078658024608708 No Hit CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC 17275 0.882824753026262 No Hit ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC 14774 0.7550131925447174 No Hit TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA 10266 0.5246355377463158 No Hit ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC 9946 0.5082821993400406 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA 9272 0.4738379803218235 No Hit CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT 9192 0.4697496457202546 No Hit CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA 8553 0.4370940730902239 No Hit CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG 8296 0.42396029818268416 No Hit TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT 8295 0.4239091940001645 No Hit CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA 7780 0.39759054000256544 No Hit CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC 7774 0.39728391490744774 No Hit AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG 6944 0.3548674434161715 No Hit ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT 5664 0.2894540897910708 No Hit AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT 5619 0.2871544015776883 No Hit ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA 5416 0.27678025252620747 Illumina Single End Adapter 1 (100% over 32bp) TCGCTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAAT 5263 0.2689613126007072 No Hit ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA 5143 0.262828810698354 Illumina Single End Adapter 1 (100% over 32bp) CTGTGCGTCTGCTCCTCCTTCTCGCGCTTCTCCTGCCGCCCTAATCCT 5098 0.2605291224849715 No Hit CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT 4615 0.23584580232799993 No Hit CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC 4477 0.22879342514029377 No Hit CTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAATCCT 4458 0.22782244567242116 No Hit TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG 3929 0.2007883331195475 No Hit CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGA 3781 0.19322491410664525 No Hit CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC 3745 0.19138516353593926 No Hit CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG 3721 0.19015866315546864 No Hit CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG 3438 0.175696179502419 No Hit ATTAGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATA 3016 0.15413021447914363 No Hit CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC 2984 0.1524948806385161 No Hit TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC 2890 0.14769108748167276 No Hit TTGTCTTTCTAGGTCTCAGCCGGTCGTCGCGACGTTCGCCCGCTCGCT 2793 0.1427339817772706 No Hit ATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAG 2686 0.13726583424767233 No Hit TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC 2600 0.13287087455098587 No Hit TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG 2570 0.13133774907539758 No Hit AGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGT 2570 0.13133774907539758 No Hit TCTGGAAGAGGTCTTGCGAAAGCCGCCTCGAGCGCGCTCCGCGGCTGC 2522 0.12888474831445632 No Hit CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA 2478 0.12663616428359345 No Hit TCGTTGTCTTTCTAGGTCTCAGCCGGTCGTCGCGACGTTCGCCCGCTC 2477 0.12658506010107387 No Hit TCGCTGTGCGTCTGCTCCTCCTTCTCGCGCTTCTCCTGCCGCCCTAAT 2343 0.11973709964344612 No Hit CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG 2343 0.11973709964344612 No Hit ACACCCGGGTGAGGGGTCTGCTGGCTGCACCTGTCGGTCTCATGGCTCC 2312 0.11815286998533821 No Hit CTCTTTTTAAGTTAGTGCTGGAACGTGGAAGAGCTGCTGCCTCCGAAG 2275 0.11626201523211264 No Hit ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA 2230 0.1139623270187302 Illumina Single End Adapter 1 (100% over 32bp) CGGCTCCTGGCCAGGTAACTTACTACTTCCAGTCAATATGCCGACCTT 2197 0.11227588899558305 No Hit CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCT 2114 0.10803424184645544 No Hit TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA 2111 0.1078809292988966 Illumina Single End Adapter 1 (100% over 31bp) AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT 2035 0.10399701142740625 No Hit AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT 2010 0.102719406864416 No Hit CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG 1971 0.1007263437461512 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.29742634226412995 0.0 0.0 0.0 0.0 2 0.5204449947797077 0.0 0.0 0.0 0.0 3 0.5904577248315734 0.0 0.0 0.0 0.0 4 0.6632811849220176 0.0 0.0 0.0 0.0 5 0.7410106465343443 0.0 0.0 0.0 0.0 6 0.8312606328639754 0.0 0.0 0.0 0.0 7 0.896827299036635 0.0 0.0 0.0 0.0 8 0.9646936534226771 0.0 0.0 0.0 0.0 9 1.0168199195926793 0.0 0.0 0.0 0.0 10 1.065062267891191 0.0 0.0 0.0 0.0 11 1.1153487834904872 0.0 0.0 0.0 0.0 12 1.1542390663879103 0.0 0.0 0.0 0.0 13 1.1924649949125787 0.0 0.0 0.0 0.0 14 1.2283401310413449 0.0 0.0 0.0 0.0 15 1.2627332458770424 0.0 0.0 0.0 0.0 16 1.292782505198573 0.0 0.0 0.0 0.0 17 1.3288620580574175 0.0 0.0 0.0 0.0 18 1.3526255029290362 0.0 0.0 0.0 0.0 19 1.3838501584485179 0.0 0.0 0.0 0.0 20 1.403371956171009 0.0 0.0 0.0 0.0 21 1.425755588114598 0.0 0.0 0.0 0.0 22 1.4430288018062263 0.0 0.0 0.0 0.0 23 1.4613240991482466 0.0 0.0 0.0 0.0 24 1.4797727090378259 0.0 0.0 0.0 0.0 25 1.4943885052384343 0.0 0.0 0.0 0.0 26 1.517487595737298 0.0 0.0 0.0 0.0 27 1.5322567044854651 0.0 0.0 0.0 0.0 28 1.5455948961230834 0.0 0.0 0.0 0.0 29 1.5669053402337607 0.0 0.0 0.0 0.0 30 1.5809589904266534 0.0 0.0 0.0 0.0 31 1.5935306193264775 0.0 0.0 0.0 0.0 32 1.604466914385674 0.0 0.0 0.0 0.0 33 1.6182650436659687 0.0 0.0 0.0 0.0 34 1.6321653813113026 0.0 0.0 0.0 0.0 35 1.6459124064090778 0.0 0.0 0.0 0.0 36 1.6591994938641763 0.0 0.0 0.0 0.0 37 1.6717200185814807 0.0 0.0 0.0 0.0 38 1.6798966877846184 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGCCA 1615 0.0 158.12675 44 CGACGGG 3230 0.0 140.48451 44 ATTAGTC 395 0.0 138.92128 44 AATCGCA 45 1.8556479E-5 93.80156 44 TATATTA 175 0.0 84.421394 44 ACTACAC 175 0.0 78.3913 44 GGCGGGC 3180 0.0 69.02378 44 CGGGCGC 2660 0.0 67.04519 44 CAATGAA 2435 0.0 65.22296 43 CGTTAAT 25 1.2975509E-4 63.368736 43 AGGTCCC 2000 0.0 62.774662 43 TTAAGCC 1570 0.0 61.804703 43 GACGGGC 1160 0.0 60.041077 44 TCGTGCG 1025 0.0 59.504795 43 ATAAGAT 875 0.0 57.937134 43 ACAACGT 155 1.2532837E-9 54.46542 44 GGTCGCT 945 0.0 53.600887 44 TATTAGT 370 0.0 53.520897 43 AGGTCGA 60 1.6534614E-9 52.807285 43 CCGACGG 3545 0.0 49.269417 43 >>END_MODULE