FastQCFastQC Report
Tue 24 May 2016
ERR841143.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841143.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5632375
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA872161.5484764419982688No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC572251.016001242814976No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT498040.8842451008677511No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA292770.5197984864289044No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGAAGCAGTGGT267660.4752169377926718No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA252210.4477862358241417No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA221800.39379480237022574No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT217580.3863024035153909No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC214810.3813844071106771No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC209820.3725249117823298No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA180100.3197585388046783No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG176470.3133136554295479No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA176000.3124791939457156Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA172410.30610532856920925Illumina Single End Adapter 1 (100% over 32bp)
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC151940.2697618677733638No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG151260.26855456179675535No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG141770.25170554162320513No Hit
TCGCTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAAT135830.24115936882753722No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC132470.235193856943119No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC126570.22471870214607514No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC124900.22175370070352204No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG116810.20739031048181275No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCT113820.2020817150846668No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG109630.19464257972880003No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT106320.18876584034266183No Hit
AGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCCGAG101440.18010164451053065No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC97950.17390532413058435No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG92370.16399831332253267No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA90990.16154819237000376Illumina Single End Adapter 1 (100% over 31bp)
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT90560.1607847488847955No Hit
ACACCCGGGTGAGGGGTCTGCTGGCTGCACCTGTCGGTCTCATGGCTCC85690.1521383075523203No Hit
CTCTCTCCTCCCGCCGCCCAAGATGCCGAAAGGAAAGAAGGCCAAGGGA85550.15188974455713622No Hit
AAGCAGTGGTATCAACGCAGAGTG83500.14825007212765487No Hit
AGCTGTTTTTCGTCTTCCCTAGGCTATTTCTGCCGGGCGCTCCGCGAAG83070.14748662864244658No Hit
ACCAGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCC77220.13710024634368273No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG76180.13525377837945804No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA75560.13415299940078565Illumina Single End Adapter 1 (100% over 32bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT71970.1277791340242793No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT71840.12754832552875117No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA69490.12337601810958965Illumina Single End Adapter 1 (100% over 31bp)
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC67120.1191682016911161No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT65990.11716194322998735No Hit
TTGTCTTTCTAGGTCTCAGCCGGTCGTCGCGACGTTCGCCCGCTCGCT64930.11527996626645065No Hit
TCTGGAAGAGGTCTTGCGAAAGCCGCCTCGAGCGCGCTCCGCGGCTGC63170.11215517432699348No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA61240.10872855589338423Illumina Single End Adapter 1 (100% over 32bp)
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC60990.10828469340198406No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC60760.10787633990989591No Hit
CTGCGCGTCTGCTCCCCCTTCTCACGTTTCTCCTGCTGCCCTAATCCT60240.10695310592778358No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA59890.10633169843982335Illumina Single End Adapter 1 (100% over 32bp)
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG57080.1013426840364855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA100750.0145.7538144
ATTAGTC25900.0141.5103944
AATCGCA4450.0108.8487744
CTATCAT14100.083.019744
CGACGGG30550.066.0634244
TTAAGCC100300.063.82446743
ACATTGG8100.061.7929544
TATTAGT27100.060.44930343
ACTTCTA12450.059.65553744
CCCATAC7450.057.43172544
AGGTCCC35050.049.1878643
ATTAAGC99900.047.52672242
CAATGAA38600.046.88841243
ATCCTTG24400.046.44792643
TAATCCT95050.045.1805342
AATCCTG33550.045.14277643
TAATCGC4700.044.57324243
GATTAAG104000.044.417541
ATAAGAT17150.044.25581743
AGATTAA102100.044.1899240
Produced by FastQC (version 0.11.5)