##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841143.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5632375
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.33384602410173	39.0	37.0	39.0	34.0	39.0
2	37.93258385672119	39.0	38.0	39.0	35.0	39.0
3	38.35317517033223	39.0	38.0	39.0	35.0	41.0
4	38.833919438957814	39.0	39.0	41.0	35.0	41.0
5	39.30798251181784	41.0	39.0	41.0	35.0	41.0
6	39.30431141392397	41.0	39.0	41.0	35.0	41.0
7	39.27161082136754	41.0	39.0	41.0	35.0	41.0
8	39.29728116469518	40.0	39.0	41.0	35.0	41.0
9	39.298469828447146	40.0	39.0	41.0	35.0	41.0
10	39.17824612175148	40.0	39.0	41.0	35.0	41.0
11	39.256697574291486	40.0	39.0	41.0	35.0	41.0
12	39.241960451852016	40.0	39.0	41.0	35.0	41.0
13	39.22457133979893	40.0	39.0	41.0	35.0	41.0
14	39.20846605561597	40.0	39.0	41.0	35.0	41.0
15	39.20657573403759	40.0	39.0	41.0	35.0	41.0
16	39.1424257085155	40.0	38.0	41.0	35.0	41.0
17	39.05743669411216	40.0	38.0	41.0	35.0	41.0
18	39.022975032734855	40.0	38.0	41.0	35.0	41.0
19	38.919039126478616	40.0	38.0	41.0	35.0	41.0
20	38.864964424421316	40.0	38.0	41.0	35.0	41.0
21	38.55895443751526	40.0	38.0	41.0	35.0	41.0
22	38.51842215761557	40.0	38.0	41.0	34.0	41.0
23	38.36200075456624	40.0	37.0	41.0	34.0	41.0
24	38.23257595596884	40.0	37.0	41.0	34.0	41.0
25	38.21806151435778	40.0	37.0	41.0	34.0	41.0
26	38.11055517272373	40.0	37.0	41.0	34.0	41.0
27	38.095192886370505	40.0	36.0	41.0	34.0	41.0
28	38.14098570953793	40.0	36.0	41.0	34.0	41.0
29	38.19599889750363	40.0	36.0	41.0	34.0	41.0
30	38.23647164982056	40.0	36.0	41.0	34.0	41.0
31	38.19906345051937	40.0	36.0	41.0	34.0	41.0
32	38.14682502701158	40.0	35.0	41.0	34.0	41.0
33	38.05465214267699	40.0	35.0	41.0	34.0	41.0
34	37.96021469937379	39.0	35.0	41.0	34.0	41.0
35	37.866140705432706	39.0	35.0	41.0	34.0	41.0
36	37.708705740233235	39.0	35.0	41.0	34.0	41.0
37	37.68360345011264	39.0	35.0	41.0	34.0	41.0
38	37.53989903415844	39.0	35.0	41.0	34.0	41.0
39	37.35551256213042	38.0	35.0	41.0	34.0	41.0
40	37.21019809069402	38.0	35.0	41.0	33.0	41.0
41	37.0192441248068	37.0	35.0	41.0	33.0	41.0
42	36.90542074784418	37.0	35.0	41.0	33.0	41.0
43	36.800791767469455	37.0	35.0	40.0	33.0	41.0
44	36.74270340659206	36.0	35.0	40.0	33.0	41.0
45	36.68662968395214	36.0	35.0	40.0	33.0	41.0
46	36.545321395003135	36.0	35.0	40.0	33.0	41.0
47	36.41505468375078	36.0	35.0	40.0	33.0	41.0
48	36.38838188289031	36.0	35.0	40.0	33.0	41.0
49	36.680856839581686	37.0	35.0	40.0	33.0	41.0
50	37.22889849444514	38.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	6.0
14	273.0
15	1459.0
16	1314.0
17	25.0
18	0.0
19	0.0
20	0.0
21	19.0
22	35.0
23	111.0
24	295.0
25	702.0
26	1676.0
27	3975.0
28	8469.0
29	16867.0
30	30846.0
31	51800.0
32	85013.0
33	145518.0
34	323402.0
35	274697.0
36	432554.0
37	660491.0
38	992989.0
39	1228292.0
40	1371545.0
41	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	46.81937903637453	19.25083113251515	33.92978983111033
2	28.546110654919104	22.62367118666637	27.890419228123125	20.939798930291396
3	32.986333473889786	15.082411948778269	22.409835992809427	29.521418584522515
4	23.79241439002197	16.91204864732906	27.022419494440626	32.27311746820835
5	27.010328680174883	20.38605384052021	27.412361570385496	25.19125590891942
6	28.815180097205882	20.943048003728446	22.485736478838856	27.756035420226816
7	32.728396102887324	20.47228744534943	22.08398766062274	24.715328791140504
8	30.936292416609334	17.509736123748862	26.520091435673226	25.033880023968575
9	27.25576688341952	19.977700348432055	24.51857342595264	28.247959342195788
10	29.090215051377083	19.85297498834861	26.0614039370603	24.99540602321401
11	30.764908231429906	21.21213520051488	22.956337246720963	25.06661932133425
12	27.483415078008832	17.487649526176792	25.932950132049093	29.095985263765282
13	25.217798885905147	17.52944361836703	26.596897401185117	30.65586009454271
14	25.3752102798553	20.914285714285715	21.343092389977585	32.367411615881394
15	23.49499101178455	22.36417141969418	24.873148538582747	29.267689029938527
16	28.54923544685856	21.283810115626178	25.349892363345834	24.81706207416942
17	28.776084688963362	20.660325351206197	19.655971060165562	30.907618899664886
18	27.393133447258037	19.48178166404048	19.201260569475576	33.9238243192259
19	26.645757784238445	21.27793337624004	26.272611464968154	25.803697374553362
20	31.986471071262123	17.20031070374398	23.61360882398633	27.199609401007567
21	27.94844093299896	21.023564659668434	22.681142502052865	28.346851905279745
22	29.776355445083112	19.990021971193325	25.592596373643445	24.641026210080117
23	29.483725781752813	18.879122927716992	23.56327481746155	28.073876473068644
24	31.773097494396236	19.66873654541823	21.47916287534122	27.079003084844317
25	26.766943548640487	20.52885123497168	24.712108920874407	27.992096295513424
26	29.78685347344679	20.24474884009732	23.72295885204753	26.245438834408365
27	28.545089529412294	21.08393523385019	24.732927605014908	25.6380476317226
28	28.468168860515437	17.9141391745859	27.154865919107667	26.462826045790997
29	32.13664509453756	19.2801208324035	25.24576169402203	23.337472379036907
30	26.605812535882322	19.767337689914218	23.587504069522307	30.039345704681153
31	26.80961859353412	19.391575761530028	30.91097365136626	22.887831993569595
32	28.586660630573213	19.854641851302656	24.405019821303707	27.153677696820427
33	26.47391357121065	21.13590546297581	23.92693441839364	28.463246547419896
34	24.286558793833148	21.016136748201973	30.23033758264021	24.466966875324665
35	28.171528528365453	21.719189799365118	22.66419549361273	27.4450861786567
36	26.282042761473235	21.579140782904794	24.941045619275112	27.197770836346862
37	24.448454216044638	22.560046678502406	23.705918371871416	29.285580733581547
38	30.503834712989775	24.235405788353777	20.660088559272385	24.600670939384063
39	26.310730410131967	25.048842183194175	22.359813047151096	26.280614359522765
40	26.87110158197595	23.85450382759188	20.870905295212907	28.403489295219263
41	26.918358670095692	23.338538678055556	21.376005172311828	28.367097479536923
42	26.515731827914244	24.59025956731539	24.598406500166156	24.295602104604203
43	24.71815306760165	24.80433653446253	24.777158093076114	25.700352304859702
44	24.655020618092323	23.9418025167586	23.83441235365659	27.56876451149249
45	25.131731317635076	22.805344692820892	27.556854526860892	24.50606946268314
46	26.123874271734635	23.924017091738104	22.648761115629206	27.303347520898047
47	29.648574753120993	20.86647443818551	21.872524821500864	27.61242598719264
48	24.704249788563594	22.639751642390962	23.896497246056054	28.75950132298939
49	27.015179207462207	22.702360591422398	21.271927118617032	29.010533082498362
50	22.174099680467585	30.85491977575875	23.59962929691498	23.37135124685869
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8247.0
1	8241.0
2	7499.0
3	6476.0
4	6010.0
5	4654.5
6	3053.5
7	2623.5
8	2598.5
9	2481.5
10	2351.0
11	2372.5
12	2397.5
13	2412.0
14	2604.5
15	3158.0
16	3690.5
17	4462.5
18	5148.5
19	6217.0
20	7486.0
21	9290.5
22	11146.0
23	13562.5
24	15961.0
25	18394.5
26	22161.0
27	26426.5
28	30208.5
29	33532.0
30	37463.0
31	45836.5
32	56166.0
33	62586.0
34	68934.0
35	77537.5
36	89081.5
37	101517.5
38	126787.0
39	171562.0
40	210950.0
41	238084.0
42	262842.5
43	309730.0
44	333701.5
45	290452.5
46	263579.0
47	275484.5
48	307875.0
49	333681.0
50	370932.0
51	422559.5
52	436936.0
53	433218.5
54	411079.0
55	382493.0
56	374113.5
57	399910.0
58	444293.0
59	429338.5
60	370965.0
61	339436.0
62	323983.0
63	309842.0
64	339760.5
65	376972.5
66	324723.5
67	263775.5
68	230062.0
69	202234.5
70	182701.5
71	166972.5
72	160128.5
73	139956.0
74	112892.5
75	96540.5
76	88120.5
77	69227.0
78	53144.0
79	46228.5
80	39007.5
81	27128.0
82	18181.0
83	14971.0
84	12128.0
85	9225.5
86	6613.0
87	4240.5
88	2429.0
89	1613.5
90	1048.5
91	627.5
92	405.5
93	343.5
94	241.0
95	103.0
96	49.0
97	45.0
98	33.5
99	21.5
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	30951.0
25	27465.0
26	32484.0
27	86298.0
28	118198.0
29	90663.0
30	19897.0
31	22170.0
32	22110.0
33	21062.0
34	23436.0
35	21821.0
36	18827.0
37	19038.0
38	20851.0
39	23652.0
40	28340.0
41	33916.0
42	44497.0
43	42639.0
44	37832.0
45	45780.0
46	84879.0
47	209486.0
48	1183314.0
49	1915713.0
50	1407056.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.197355123251391
#Duplication Level	Percentage of deduplicated	Percentage of total
1	45.0960857002359	5.500529719543562
2	10.566666631512463	2.5777077074713612
3	6.641764334759063	2.430358747080055
4	5.275755499959514	2.5740105350581155
5	4.505223225890087	2.7475903797850805
6	3.8824847114289422	2.8413626871535804
7	3.195039041237438	2.7279718073037973
8	2.741084783851399	2.6747187625261035
9	2.276261732382554	2.4987935433003687
>10	14.646238891174956	31.22079014713361
>50	0.6705734872038205	5.641946482331024
>100	0.4130059123054455	9.654410930308597
>500	0.04490802404750158	3.8747737217707656
>1k	0.03714426392781006	8.81108603903599
>5k	0.003805764746701851	3.38604261056571
>10k+	0.0039579953365699245	10.837906179632567
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	87216	1.5484764419982688	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	57225	1.016001242814976	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	49804	0.8842451008677511	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	29277	0.5197984864289044	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTAATAAAGGGAAGCAGTGGT	26766	0.4752169377926718	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	25221	0.4477862358241417	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	22180	0.39379480237022574	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	21758	0.3863024035153909	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC	21481	0.3813844071106771	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	20982	0.3725249117823298	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	18010	0.3197585388046783	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	17647	0.3133136554295479	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	17600	0.3124791939457156	Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	17241	0.30610532856920925	Illumina Single End Adapter 1 (100% over 32bp)
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	15194	0.2697618677733638	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG	15126	0.26855456179675535	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	14177	0.25170554162320513	No Hit
TCGCTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAAT	13583	0.24115936882753722	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	13247	0.235193856943119	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	12657	0.22471870214607514	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	12490	0.22175370070352204	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	11681	0.20739031048181275	No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCT	11382	0.2020817150846668	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	10963	0.19464257972880003	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	10632	0.18876584034266183	No Hit
AGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCCGAG	10144	0.18010164451053065	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	9795	0.17390532413058435	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG	9237	0.16399831332253267	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	9099	0.16154819237000376	Illumina Single End Adapter 1 (100% over 31bp)
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	9056	0.1607847488847955	No Hit
ACACCCGGGTGAGGGGTCTGCTGGCTGCACCTGTCGGTCTCATGGCTCC	8569	0.1521383075523203	No Hit
CTCTCTCCTCCCGCCGCCCAAGATGCCGAAAGGAAAGAAGGCCAAGGGA	8555	0.15188974455713622	No Hit
AAGCAGTGGTATCAACGCAGAGTG	8350	0.14825007212765487	No Hit
AGCTGTTTTTCGTCTTCCCTAGGCTATTTCTGCCGGGCGCTCCGCGAAG	8307	0.14748662864244658	No Hit
ACCAGCGAGCGGCCTTGAGAGGCTCTGGCTCTTGCTTCTTAGGCGGCCC	7722	0.13710024634368273	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	7618	0.13525377837945804	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	7556	0.13415299940078565	Illumina Single End Adapter 1 (100% over 32bp)
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	7197	0.1277791340242793	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	7184	0.12754832552875117	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	6949	0.12337601810958965	Illumina Single End Adapter 1 (100% over 31bp)
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC	6712	0.1191682016911161	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	6599	0.11716194322998735	No Hit
TTGTCTTTCTAGGTCTCAGCCGGTCGTCGCGACGTTCGCCCGCTCGCT	6493	0.11527996626645065	No Hit
TCTGGAAGAGGTCTTGCGAAAGCCGCCTCGAGCGCGCTCCGCGGCTGC	6317	0.11215517432699348	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA	6124	0.10872855589338423	Illumina Single End Adapter 1 (100% over 32bp)
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC	6099	0.10828469340198406	No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC	6076	0.10787633990989591	No Hit
CTGCGCGTCTGCTCCCCCTTCTCACGTTTCTCCTGCTGCCCTAATCCT	6024	0.10695310592778358	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	5989	0.10633169843982335	Illumina Single End Adapter 1 (100% over 32bp)
ATTCCTGCTCACCGCAGTGTAGACAGGGAAACTTTCTTTGCAGTTCAGG	5708	0.1013426840364855	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.32471204420870414	0.0	0.0	0.0	0.0
2	0.5549346412481413	0.0	0.0	0.0	0.0
3	0.6374398011496039	0.0	0.0	0.0	0.0
4	0.7388002396857454	0.0	0.0	0.0	0.0
5	0.8589449388579418	0.0	0.0	0.0	0.0
6	0.9738875696309284	0.0	0.0	0.0	0.0
7	1.0629796489047694	0.0	0.0	0.0	0.0
8	1.162600146474622	0.0	0.0	0.0	0.0
9	1.235482367562529	0.0	0.0	0.0	0.0
10	1.3014935972835615	0.0	0.0	0.0	0.0
11	1.3744113273707805	0.0	0.0	0.0	0.0
12	1.427124436849464	0.0	0.0	0.0	0.0
13	1.4812934152999402	0.0	0.0	0.0	0.0
14	1.540753234647906	0.0	0.0	0.0	0.0
15	1.6105639272953238	0.0	0.0	0.0	0.0
16	1.6553407754277725	0.0	0.0	0.0	0.0
17	1.7394971037972435	0.0	0.0	0.0	0.0
18	1.7827115559599636	0.0	0.0	0.0	0.0
19	1.8697618677733638	0.0	0.0	0.0	0.0
20	1.9013471226613996	0.0	0.0	0.0	0.0
21	1.9466921147828402	0.0	0.0	0.0	0.0
22	1.9689740118511285	0.0	0.0	0.0	0.0
23	2.0001154042477642	0.0	0.0	0.0	0.0
24	2.028806675691871	0.0	0.0	0.0	0.0
25	2.0526332142302315	0.0	0.0	0.0	0.0
26	2.100321800306265	0.0	0.0	0.0	0.0
27	2.119230342439912	0.0	0.0	0.0	0.0
28	2.140784305022304	0.0	0.0	0.0	0.0
29	2.164717370558601	0.0	0.0	0.0	0.0
30	2.1821700437204554	0.0	0.0	0.0	0.0
31	2.197279122927717	0.0	0.0	0.0	0.0
32	2.211660267649082	0.0	0.0	0.0	0.0
33	2.2262367118666635	0.0	0.0	0.0	0.0
34	2.2410439645797733	0.0	0.0	0.0	0.0
35	2.255797953793915	0.0	0.0	0.0	0.0
36	2.2705519430080563	0.0	0.0	0.0	0.0
37	2.2835659912559088	0.0	0.0	0.0	0.0
38	2.294680308040569	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	10075	0.0	145.75381	44
ATTAGTC	2590	0.0	141.51039	44
AATCGCA	445	0.0	108.84877	44
CTATCAT	1410	0.0	83.0197	44
CGACGGG	3055	0.0	66.06342	44
TTAAGCC	10030	0.0	63.824467	43
ACATTGG	810	0.0	61.79295	44
TATTAGT	2710	0.0	60.449303	43
ACTTCTA	1245	0.0	59.655537	44
CCCATAC	745	0.0	57.431725	44
AGGTCCC	3505	0.0	49.18786	43
ATTAAGC	9990	0.0	47.526722	42
CAATGAA	3860	0.0	46.888412	43
ATCCTTG	2440	0.0	46.447926	43
TAATCCT	9505	0.0	45.18053	42
AATCCTG	3355	0.0	45.142776	43
TAATCGC	470	0.0	44.573242	43
GATTAAG	10400	0.0	44.4175	41
ATAAGAT	1715	0.0	44.255817	43
AGATTAA	10210	0.0	44.18992	40
>>END_MODULE