FastQCFastQC Report
Tue 24 May 2016
ERR841142.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841142.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6508824
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA3411965.242052942282662No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG400230.6149037061072784No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC361530.5554459607449825No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA284320.4368223814317302No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGTTTGGGAAGCAGTGGT271730.41747940949086965No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC262750.40368275436545836No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC253970.390193374409878No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA240470.36945230044628646No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGG229160.3520758895923442No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG222480.3418128989199892No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA222240.34144416871619204No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT215450.33101217670043004No Hit
AAGCAGTGGTATCAACGCAGAGTG202060.3104401040802455No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC197560.30352641275904835No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC191590.2943542489395934No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC185590.2851359938446638No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC181790.27929776561787506No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC179330.2755182810289539No Hit
TACCTGGTTGATCCTGCCAGTAGCATATG158690.24380748350239612No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG144440.22191412765193833No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG140730.21621417325157355No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT137580.21137458932673553No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCT134190.20616627519810032No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC132040.20286306712241722No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG130180.20000540804298905No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT129050.19826930333344395No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA127790.19633346976350874Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA121210.18622411667606928Illumina Single End Adapter 1 (100% over 32bp)
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG116760.17938724414732984No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG115630.17765113943778477No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA115000.17668322265281716No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT109860.1687862507881608No Hit
CAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG100090.15377585874191713No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG98910.1519629352399143No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT97740.15016537549640305No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCT93160.14312877410727345No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG92550.14219158483928893No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG89960.13821237138997766No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTG88460.13590780761624527No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA85400.13120649751783117No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCTG84410.1296854854271678No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT83440.1281952008534875No Hit
AAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATG79470.12209578873234243No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC78790.12105105315491707No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA78180.12011386388693257Illumina Single End Adapter 1 (100% over 31bp)
CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGA76680.11780930011320016No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC76530.11757884373582693No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT75510.1160117403696889No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT73340.11267780477702269No Hit
ACACCCGGGTGAGGGGTCTGCTGGCTGCACCTGTCGGTCTCATGGCTCC68880.10582556848979169No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA385800.0101.4888844
TTAAGCC383700.059.57229643
ATTAGTC6100.059.3198444
CGACGGG85150.055.49440444
ATTAAGC383650.048.9926742
GATTAAG392600.046.5409341
CAATGAA53000.046.1857943
AGATTAA389250.046.10626640
AAGATTA394600.045.2134239
AAAGATT396200.044.7375638
CAAAGAT395750.044.47715437
GTCTCAA390200.044.12587733
CTCAAAG395050.043.89190335
TCTCAAA394800.043.67105534
TCAAAGA400450.043.575836
AATGAAG37750.043.4164844
AGGTCCC38650.043.367243
TGTCTCA395800.043.2775532
TATGCTT387550.043.1560926
TTGTCTC395500.043.1481631
Produced by FastQC (version 0.11.5)