##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841142.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6508824
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.36418253128368	39.0	37.0	39.0	34.0	39.0
2	37.97107603462622	39.0	38.0	39.0	35.0	39.0
3	38.255182042101616	39.0	38.0	39.0	35.0	40.0
4	38.601352256567395	39.0	38.0	40.0	35.0	41.0
5	39.310164170977735	40.0	39.0	41.0	36.0	41.0
6	39.26436557510235	40.0	39.0	41.0	36.0	41.0
7	39.12067756018599	40.0	39.0	41.0	35.0	41.0
8	39.169321524134006	40.0	39.0	41.0	35.0	41.0
9	39.1987637090817	40.0	39.0	41.0	35.0	41.0
10	38.97935525680215	40.0	38.0	41.0	35.0	41.0
11	39.12909551710109	40.0	39.0	41.0	35.0	41.0
12	39.13895305818686	40.0	39.0	41.0	35.0	41.0
13	39.1520638751332	40.0	39.0	41.0	35.0	41.0
14	39.143533609143525	40.0	38.0	41.0	35.0	41.0
15	39.14049527226424	40.0	38.0	41.0	35.0	41.0
16	39.06097445560058	40.0	38.0	41.0	35.0	41.0
17	38.95385863867267	40.0	38.0	41.0	35.0	41.0
18	38.913653833626476	40.0	38.0	41.0	35.0	41.0
19	38.78051626530384	40.0	38.0	41.0	35.0	41.0
20	38.752213610323466	40.0	38.0	41.0	35.0	41.0
21	38.39198110134795	40.0	38.0	41.0	34.0	41.0
22	38.37926697664586	40.0	38.0	41.0	34.0	41.0
23	38.14818867432888	40.0	37.0	41.0	34.0	41.0
24	38.070755792444224	40.0	37.0	41.0	33.0	41.0
25	38.07771402283507	40.0	37.0	41.0	33.0	41.0
26	37.99132362035905	40.0	36.0	41.0	33.0	41.0
27	37.954817596482584	40.0	36.0	41.0	33.0	41.0
28	37.9880085971844	40.0	36.0	41.0	34.0	41.0
29	38.11471558577714	40.0	36.0	41.0	34.0	41.0
30	38.188452497721954	40.0	36.0	41.0	34.0	41.0
31	38.15719824233635	40.0	36.0	41.0	34.0	41.0
32	38.1340081235467	40.0	36.0	41.0	34.0	41.0
33	38.10663219043465	40.0	35.0	41.0	34.0	41.0
34	38.040853162415566	40.0	35.0	41.0	34.0	41.0
35	37.913914158561354	39.0	35.0	41.0	34.0	41.0
36	37.83460756667418	39.0	35.0	41.0	34.0	41.0
37	37.79149410483963	39.0	35.0	41.0	34.0	41.0
38	37.690385382537556	39.0	35.0	41.0	34.0	41.0
39	37.568029364666835	39.0	35.0	41.0	34.0	41.0
40	37.385957805354565	38.0	35.0	41.0	33.0	41.0
41	37.229802526030454	38.0	35.0	41.0	33.0	41.0
42	37.150934174368544	37.0	35.0	41.0	33.0	41.0
43	37.03334442481288	37.0	35.0	41.0	33.0	41.0
44	36.93664378887246	37.0	35.0	41.0	33.0	41.0
45	36.841663705009616	37.0	35.0	40.0	33.0	41.0
46	36.7466108607145	36.0	35.0	40.0	33.0	41.0
47	36.619919190530226	36.0	35.0	40.0	33.0	41.0
48	36.556539144863216	36.0	35.0	40.0	33.0	41.0
49	36.803486122755025	37.0	35.0	40.0	33.0	41.0
50	37.109054726607255	38.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	124.0
15	1012.0
16	1069.0
17	28.0
18	0.0
19	0.0
20	6.0
21	15.0
22	41.0
23	120.0
24	278.0
25	861.0
26	2186.0
27	5390.0
28	11456.0
29	23192.0
30	42041.0
31	69389.0
32	110088.0
33	177785.0
34	351828.0
35	345226.0
36	516489.0
37	751937.0
38	1114841.0
39	1388098.0
40	1595322.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	49.13646459022398	19.647251177785726	31.216284231990294
2	27.215453974481413	29.08361018826135	25.128072290785553	18.572863546471684
3	32.460902307390704	14.69403689514419	20.89202289077105	31.95303790669405
4	22.007063027053736	16.58540774800486	25.89168181533254	35.51584740960887
5	24.377230049545048	21.960910296545123	30.64533623892734	23.016523414982494
6	34.559146168340085	21.898579528344904	20.42097005542015	23.12130424789486
7	36.046004623876755	19.035896499889997	23.25927079914897	21.658828077084276
8	32.23703698241034	17.3820032620332	27.134271874612065	23.246687880944393
9	27.563074374111206	20.304697131156104	28.48849500309119	23.6437334916415
10	32.463237598681424	17.97645780558823	25.622232218907747	23.938072376822603
11	28.34384521689325	27.109275039546315	22.463044015324428	22.083835728236007
12	26.72298405979329	16.78174121776837	31.13488703950207	25.360387682936274
13	24.017011367952183	16.6829676144262	24.638705855312725	34.661315162308895
14	22.68182393624409	23.630198020410447	21.069674030208837	32.61830401313663
15	22.811063872675003	22.71880450293325	29.020941417374324	25.449190207017423
16	31.377987790113853	21.33377703867857	21.74123927763295	25.546995893574632
17	29.49883112525396	19.777105664556302	19.685445481395718	31.038617728794023
18	26.888881923985043	19.728448641413564	18.734229101908426	34.64844033269297
19	25.138181643872993	27.685830804458682	23.819602435094268	23.356385116574057
20	38.395630301264866	16.30294504813773	19.951330685850472	25.350093964746932
21	26.81756335706727	21.404772966668016	26.43121092228028	25.346452753984437
22	29.59600689771301	23.933002336520392	23.252756565548555	23.218234200218042
23	32.67872967528389	18.850962938927214	23.788429369114912	24.68187801667398
24	31.535297313308824	19.261682294681805	20.398124146543214	28.804896245466153
25	26.048882664917734	24.0131807569493	23.79458501094449	26.14335156718847
26	27.029377837893666	21.15045051659153	27.328597133268545	24.49157451224626
27	28.13820890151071	25.202447172217397	23.15725897935394	23.502084946917957
28	26.39167081111807	17.63165470091872	30.488885517609976	25.487788970353236
29	36.614512117368605	19.359113330609638	22.507600952193336	21.518773599828428
30	26.241481693985218	20.426074908857547	21.436157996283825	31.89628540087341
31	26.12189956021681	19.200894424488027	32.43859721465637	22.238608800638797
32	27.54542666757578	18.283538851518724	27.68820842980031	26.482826051105192
33	32.44054993629808	19.537416692941882	22.594990807613442	25.427042563146596
34	24.366293631765775	20.17767463514698	31.235155421224487	24.22087631186276
35	25.59490288578169	21.08862814320363	22.906267085763332	30.410201885251354
36	25.904995660468465	20.67147601383571	29.459928226996794	23.963600098699033
37	24.550902947502685	20.58360701250161	23.07679854532253	31.78869149467318
38	27.00303234151937	27.870820591991052	20.921514483820943	24.20463258266864
39	25.78536244755969	28.59343502535016	20.365754102480455	25.255448424609696
40	26.222224621751884	28.529854232056266	20.2199598527726	25.02796129341925
41	30.653057417911363	22.30312050480715	21.357823168769652	25.68599890851184
42	27.491379430165335	27.336465164347235	22.085144258521915	23.08701114696552
43	24.505968491693437	21.75189742259529	29.05143753370853	24.690696552002738
44	25.436316296284534	21.03637174967398	27.846237100351544	25.681074853689946
45	25.74726290782377	26.944748595743334	24.591354213344957	22.716634283087938
46	26.543939217471678	28.566744982653773	21.05451441159673	23.834801388277814
47	35.46391911773222	19.441573069441766	20.44243543174828	24.652072381077737
48	24.122837905487252	23.11767592243227	21.14309859832321	31.616387573757272
49	26.72254504157092	21.485881358957357	19.698641704386745	32.09293189508498
50	23.388236556840194	35.24643573591506	21.188743497538386	20.176584209706363
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2689.0
1	3017.0
2	5263.5
3	7015.5
4	6547.5
5	4718.5
6	2797.5
7	2422.5
8	2518.0
9	2638.5
10	2637.0
11	2624.5
12	2645.5
13	2743.0
14	2834.5
15	3170.0
16	3687.0
17	4820.5
18	5932.5
19	7493.0
20	9068.0
21	11574.0
22	14250.5
23	17580.5
24	21053.5
25	24151.5
26	28823.0
27	35115.0
28	40629.0
29	46007.5
30	52595.0
31	60969.0
32	69972.0
33	79440.5
34	88543.0
35	96572.5
36	108408.5
37	122986.5
38	146326.0
39	185144.0
40	221854.0
41	258635.0
42	297991.5
43	483457.5
44	641588.0
45	478631.5
46	328317.5
47	315656.5
48	329431.5
49	374272.5
50	442659.5
51	497838.0
52	509248.0
53	530988.5
54	538805.5
55	474503.5
56	409113.0
57	400811.5
58	420851.0
59	429418.0
60	397579.0
61	366258.0
62	346551.5
63	329963.5
64	345411.5
65	367777.0
66	324357.5
67	266677.0
68	231363.0
69	208193.0
70	189294.5
71	178342.0
72	180167.0
73	160373.0
74	125537.0
75	109708.0
76	105105.0
77	79643.5
78	56920.5
79	54810.5
80	52199.0
81	35093.5
82	20522.5
83	17732.5
84	14953.0
85	11679.0
86	8808.5
87	5520.0
88	2858.5
89	1968.0
90	1337.0
91	843.0
92	505.0
93	400.5
94	267.5
95	119.0
96	85.0
97	81.0
98	90.5
99	100.0
100	100.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	48497.0
25	31263.0
26	38014.0
27	89452.0
28	118820.0
29	152383.0
30	40398.0
31	35455.0
32	30074.0
33	26271.0
34	47200.0
35	23179.0
36	21445.0
37	20745.0
38	19787.0
39	23975.0
40	26733.0
41	31344.0
42	39817.0
43	39265.0
44	39129.0
45	44781.0
46	81088.0
47	200056.0
48	947951.0
49	1794763.0
50	2496939.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.559165916850688
#Duplication Level	Percentage of deduplicated	Percentage of total
1	44.52778410502865	5.14704044379733
2	9.796422388562293	2.264769435618845
3	6.597473234875566	2.287838632617249
4	5.294184525823098	2.4478542931365075
5	4.67058114368205	2.699401118396753
6	3.8511627128985655	2.670973726271
7	3.2643887295976497	2.6413527659761664
8	2.7834757228298295	2.573972616457272
9	2.3966064442704162	2.493249437404378
>10	15.738024789107225	31.581716507508446
>50	0.6227171640292267	4.933638553415581
>100	0.3736163611553161	8.37312323389425
>500	0.041573472013783384	3.219107514895856
>1k	0.03242730770166353	7.567934872852271
>5k	0.004988816569486695	3.996676944684375
>10k+	0.004573081855362804	15.101349903073583
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	341196	5.242052942282662	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	40023	0.6149037061072784	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	36153	0.5554459607449825	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	28432	0.4368223814317302	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGTTTGGGAAGCAGTGGT	27173	0.41747940949086965	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	26275	0.40368275436545836	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	25397	0.390193374409878	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	24047	0.36945230044628646	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGG	22916	0.3520758895923442	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG	22248	0.3418128989199892	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	22224	0.34144416871619204	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	21545	0.33101217670043004	No Hit
AAGCAGTGGTATCAACGCAGAGTG	20206	0.3104401040802455	No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC	19756	0.30352641275904835	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC	19159	0.2943542489395934	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	18559	0.2851359938446638	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	18179	0.27929776561787506	No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC	17933	0.2755182810289539	No Hit
TACCTGGTTGATCCTGCCAGTAGCATATG	15869	0.24380748350239612	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTG	14444	0.22191412765193833	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG	14073	0.21621417325157355	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	13758	0.21137458932673553	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCT	13419	0.20616627519810032	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC	13204	0.20286306712241722	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	13018	0.20000540804298905	No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT	12905	0.19826930333344395	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	12779	0.19633346976350874	Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	12121	0.18622411667606928	Illumina Single End Adapter 1 (100% over 32bp)
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	11676	0.17938724414732984	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	11563	0.17765113943778477	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	11500	0.17668322265281716	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	10986	0.1687862507881608	No Hit
CAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG	10009	0.15377585874191713	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	9891	0.1519629352399143	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	9774	0.15016537549640305	No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCT	9316	0.14312877410727345	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG	9255	0.14219158483928893	No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG	8996	0.13821237138997766	No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCTG	8846	0.13590780761624527	No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA	8540	0.13120649751783117	No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCTG	8441	0.1296854854271678	No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT	8344	0.1281952008534875	No Hit
AAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATG	7947	0.12209578873234243	No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC	7879	0.12105105315491707	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	7818	0.12011386388693257	Illumina Single End Adapter 1 (100% over 31bp)
CCCTTCCACCTTCTGCCGTGTTAGGATTTGGTGTTCCTCCCTTCCAGA	7668	0.11780930011320016	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	7653	0.11757884373582693	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	7551	0.1160117403696889	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	7334	0.11267780477702269	No Hit
ACACCCGGGTGAGGGGTCTGCTGGCTGCACCTGTCGGTCTCATGGCTCC	6888	0.10582556848979169	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.37808673271853716	0.0	0.0	0.0	0.0
2	0.528328927007398	0.0	0.0	0.0	0.0
3	0.5748196602028262	0.0	0.0	0.0	0.0
4	0.6307283773535741	0.0	0.0	0.0	0.0
5	0.7274278732993855	0.0	0.0	0.0	0.0
6	0.8289515894115435	0.0	0.0	0.0	0.0
7	0.9174007470473929	0.0	0.0	0.0	0.0
8	1.0167889007292255	0.0	0.0	0.0	0.0
9	1.0812091400842918	0.0	0.0	0.0	0.0
10	1.142633446533506	0.0	0.0	0.0	0.0
11	1.2237540913688862	0.0	0.0	0.0	0.0
12	1.272687662164471	0.0	0.0	0.0	0.0
13	1.3254160813074682	0.0	0.0	0.0	0.0
14	1.3851196468056288	0.0	0.0	0.0	0.0
15	1.454656017738381	0.0	0.0	0.0	0.0
16	1.4926966837634572	0.0	0.0	0.0	0.0
17	1.5909325555584235	0.0	0.0	0.0	0.0
18	1.624394821553018	0.0	0.0	0.0	0.0
19	1.7213401376346942	0.0	0.0	0.0	0.0
20	1.743940226375763	0.0	0.0	0.0	0.0
21	1.7933654374430772	0.0	0.0	0.0	0.0
22	1.811310307361207	0.0	0.0	0.0	0.0
23	1.8408701786989479	0.0	0.0	0.0	0.0
24	1.8767445547767154	0.0	0.0	0.0	0.0
25	1.8994521898272254	0.0	0.0	0.0	0.0
26	1.9714160346016423	0.0	0.0	0.0	0.0
27	1.9910048266783678	0.0	0.0	0.0	0.0
28	2.0141580107251325	0.0	0.0	0.0	0.0
29	2.045162075361079	0.0	0.0	0.0	0.0
30	2.0609252915734086	0.0	0.0	0.0	0.0
31	2.075843501068703	0.0	0.0	0.0	0.0
32	2.089240698473334	0.0	0.0	0.0	0.0
33	2.1029758985647793	0.0	0.0	0.0	0.0
34	2.117848016784599	0.0	0.0	0.0	0.0
35	2.1316753994269932	0.0	0.0	0.0	0.0
36	2.1454413270354213	0.0	0.0	0.0	0.0
37	2.1590536170589343	0.0	0.0	0.0	0.0
38	2.1705610721691047	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	38580	0.0	101.48888	44
TTAAGCC	38370	0.0	59.572296	43
ATTAGTC	610	0.0	59.31984	44
CGACGGG	8515	0.0	55.494404	44
ATTAAGC	38365	0.0	48.99267	42
GATTAAG	39260	0.0	46.54093	41
CAATGAA	5300	0.0	46.18579	43
AGATTAA	38925	0.0	46.106266	40
AAGATTA	39460	0.0	45.21342	39
AAAGATT	39620	0.0	44.73756	38
CAAAGAT	39575	0.0	44.477154	37
GTCTCAA	39020	0.0	44.125877	33
CTCAAAG	39505	0.0	43.891903	35
TCTCAAA	39480	0.0	43.671055	34
TCAAAGA	40045	0.0	43.5758	36
AATGAAG	3775	0.0	43.41648	44
AGGTCCC	3865	0.0	43.3672	43
TGTCTCA	39580	0.0	43.27755	32
TATGCTT	38755	0.0	43.15609	26
TTGTCTC	39550	0.0	43.14816	31
>>END_MODULE