FastQCFastQC Report
Tue 24 May 2016
ERR841141.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841141.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2831172
Sequences flagged as poor quality0
Sequence length24-50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA331471.1707872216876969No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA221780.7833504993691658No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA145970.5155815330188346No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG126300.44610500527696656No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC121520.42922153793552636No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC109030.3851055322672024No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC104890.3704826128543232No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG104010.3673743594525518No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC103930.3670917909614817No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA96960.34247301117699663No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC90190.318560652620187No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA86220.3045381912508318No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT82960.293023525239724No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG68040.24032450165514496No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA66960.2365098270256982Illumina Single End Adapter 1 (100% over 32bp)
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG63140.2230171815770995No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA62260.2199089281753281Illumina Single End Adapter 1 (100% over 32bp)
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT61770.21817819616752354No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC57440.2028841765883528No Hit
CTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAATCCT56010.19783326481047422No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG54740.19334749001473595No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC54490.1924644634801418No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT48680.17194292681617365No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG48660.17187228469340612No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG47550.16795164687980807No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT43300.15294019579170745No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA41530.14668836792678083No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC37890.13383150158308998No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT37790.13347829096925232No Hit
CTGTGCGTCTGCTCCTCCTTCTCGCGCTTCTCCTGCCGCCCTAATCCT35190.12429481500947312No Hit
CTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAATCCTG34970.12351775165903026No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT34770.12281133043135492No Hit
CAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG34510.121892982835377No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT32830.11595904452290429No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT32220.11380445977849456No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG31790.11228565413899261No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC31510.11129666442024717No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG31470.11115538017471209No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA30190.10663428431759003Illumina Single End Adapter 1 (100% over 32bp)
TCTGGAAGAGGTCTTGCGAAAGCCGCCTCGAGCGCGCTCCGCGGCTGC29880.10553933141469328No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC29860.10546868929192574No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCT29480.10412648895934264No Hit
CTGTGCGTCTGCTCCTCCTTCTCGCGCTTCTCCTGCCGCCCTAATCCTG28660.1012301619258738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA40700.0106.2145444
ATTAGTC5550.092.33944
AATGAAG20250.065.20460544
CGACGGG27650.065.1981544
TTAAGCC39750.058.1584943
CAATGAA40000.055.39021343
AATCCCG8900.049.716943
AGGTCCC21500.049.06506743
TATTAGT5700.048.3769643
AATCCTG19100.046.500943
ATTAAGC39850.045.91326542
TAATCCT37400.045.80841442
GCAATGA40250.043.82229242
CTAATCC58600.043.72786741
TCGTGCG11400.043.03299343
AGATTAA40650.042.78323440
GATTAAG41950.042.51042641
TGTGTTA19250.042.46195642
ATAAGAT12100.041.6034443
AAGATTA41950.041.29281239
Produced by FastQC (version 0.11.5)