##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841141.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2831172
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.132109246630016	39.0	37.0	39.0	33.0	39.0
2	37.80228965248314	39.0	37.0	39.0	35.0	39.0
3	38.09773267042765	39.0	38.0	39.0	35.0	40.0
4	38.39799030224938	39.0	38.0	40.0	35.0	41.0
5	38.97321603915269	40.0	38.0	41.0	35.0	41.0
6	38.96074346595685	40.0	38.0	41.0	35.0	41.0
7	38.9577224555767	40.0	38.0	41.0	35.0	41.0
8	38.931831764371786	40.0	38.0	41.0	35.0	41.0
9	38.91641164860348	40.0	38.0	41.0	35.0	41.0
10	38.893887054548436	40.0	38.0	41.0	35.0	41.0
11	38.933869083192405	40.0	38.0	41.0	35.0	41.0
12	38.87159204739239	40.0	38.0	41.0	35.0	41.0
13	38.845359448313275	40.0	38.0	41.0	35.0	41.0
14	38.81994417859459	40.0	38.0	41.0	35.0	41.0
15	38.80151824050252	40.0	38.0	41.0	35.0	41.0
16	38.72747152062821	40.0	38.0	41.0	35.0	41.0
17	38.64271828062724	40.0	38.0	41.0	35.0	41.0
18	38.556959096798074	40.0	38.0	41.0	35.0	41.0
19	38.44879081878459	40.0	37.0	41.0	35.0	41.0
20	38.38673347998638	40.0	37.0	41.0	34.0	41.0
21	38.088091786722956	40.0	37.0	41.0	34.0	41.0
22	37.983444665318814	40.0	36.0	41.0	33.0	41.0
23	37.86618333326269	39.0	36.0	41.0	33.0	41.0
24	37.733851210735345	39.0	36.0	41.0	33.0	41.0
25	37.66172398845624	39.0	35.0	41.0	33.0	41.0
26	37.517638783314716	39.0	35.0	40.0	33.0	41.0
27	37.46760014346084	39.0	35.0	40.0	33.0	41.0
28	37.56013868575172	39.0	35.0	40.0	33.0	41.0
29	37.607273469160646	39.0	35.0	41.0	33.0	41.0
30	37.64215987899804	39.0	35.0	41.0	33.0	41.0
31	37.5738407879476	39.0	35.0	41.0	33.0	41.0
32	37.54096940330836	39.0	35.0	41.0	33.0	41.0
33	37.462980086358186	39.0	35.0	41.0	33.0	41.0
34	37.39438647910243	38.0	35.0	41.0	33.0	41.0
35	37.26500374992163	38.0	35.0	41.0	33.0	41.0
36	37.15239576314458	37.0	35.0	41.0	33.0	41.0
37	37.05715568625172	37.0	35.0	41.0	33.0	41.0
38	36.95391493729701	37.0	35.0	40.0	33.0	41.0
39	36.807942169864425	37.0	35.0	40.0	33.0	41.0
40	36.65262699933596	36.0	35.0	40.0	33.0	41.0
41	36.5014231474587	36.0	35.0	40.0	33.0	41.0
42	36.4120608609155	36.0	35.0	40.0	33.0	41.0
43	36.320729105913536	36.0	35.0	40.0	33.0	41.0
44	36.24036706016527	35.0	35.0	40.0	33.0	41.0
45	36.149428990551215	35.0	35.0	39.0	33.0	41.0
46	36.03141178174254	35.0	35.0	39.0	33.0	41.0
47	35.96787233224123	35.0	35.0	39.0	33.0	41.0
48	35.90285603204627	35.0	35.0	39.0	33.0	41.0
49	36.157543536378896	36.0	35.0	39.0	33.0	41.0
50	36.474939868007276	37.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	30.0
14	220.0
15	1422.0
16	292.0
17	3.0
18	0.0
19	0.0
20	0.0
21	12.0
22	16.0
23	64.0
24	164.0
25	459.0
26	1103.0
27	2571.0
28	5199.0
29	10313.0
30	19006.0
31	32840.0
32	54845.0
33	94751.0
34	186408.0
35	196352.0
36	287847.0
37	420086.0
38	505506.0
39	555953.0
40	455710.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	47.972571076571825	15.606858219846764	36.42057070358141
2	29.938166949941582	21.31368210762186	28.021999369872262	20.726151572564294
3	32.71609778565202	14.357905489316792	23.154651148005136	29.771345577026054
4	23.778703660533516	16.737626678986654	28.561528582509293	30.92214107797054
5	25.931275104444378	19.808086545077444	28.883656662329237	25.376981688148938
6	28.50278965742809	22.983661889846324	23.582601127730847	24.930947324994737
7	33.82680388192593	20.627747095549122	22.045711104800414	23.499737917724534
8	30.626786362679486	17.67480040068212	25.220332780911935	26.47808045572646
9	25.895600832446775	20.26464658452401	26.160932645561623	27.67881993746759
10	30.473281029905635	18.405522518589475	25.183280987520362	25.937915463984528
11	30.965374057104267	19.12264602786408	25.259574480109297	24.652405434922358
12	28.70397842307002	17.32148382366031	26.236872927536727	27.73766482573295
13	25.445822436785896	17.069256124318834	28.857448434782484	28.627473004112787
14	24.471490958514707	21.325726589553724	22.675944803070955	31.52683764886061
15	23.9484566815439	21.16981942460578	26.17220006414305	28.709523829707273
16	28.614227606093873	23.242423985543795	24.802060772005376	23.34128763635696
17	28.562941424964645	20.863020685426388	20.616620961213236	29.95741692839573
18	28.210649158722962	20.167407702534497	21.226333122819806	30.395610015922735
19	27.40970877078468	19.81338470428501	26.500014834845782	26.27689169008453
20	31.34020116050879	17.9207056300359	22.788477704639636	27.950615504815673
21	28.435538356553398	19.438275032389413	23.80145042406466	28.324736186992528
22	28.86483053661169	20.91056283404894	25.299451958411566	24.9251546709278
23	29.781306116336275	19.71939535994281	22.60657423851324	27.89272428520768
24	30.33658852235046	20.16846733437601	21.796521016737945	27.69842312653558
25	27.651140903428374	20.387899710269853	26.291386661052652	25.669572725249125
26	29.567857923781848	19.824873111837437	24.272495383371865	26.334773581008847
27	28.46936683720892	20.989585446263394	24.901765580813585	25.639282135714105
28	29.06184515922054	18.679291745309698	25.602376222930218	26.656486872539542
29	32.20991718676101	20.184841375089256	24.2987582650951	23.30648317305463
30	27.508623511724338	21.051447052547346	22.64278049904217	28.797148936686146
31	27.213286798437743	20.314940075921083	29.205530306028326	23.266242819612845
32	28.801398461417392	19.380043961685946	24.24115666450449	27.577400912392168
33	28.257826833960394	20.562517601216104	23.55424474188848	27.625410822935027
34	24.673742701683178	21.547736269764588	27.882844775645623	25.895676252906615
35	27.285923591380058	22.340477404883956	23.221457446322717	27.152141557413273
36	27.58016769914565	21.040926891783613	25.248171886572646	26.130733522498094
37	25.651028510548823	21.19957852309092	23.619442709530084	29.52995025683018
38	28.723769644006175	23.309549237933773	22.36754553853372	25.599135579526333
39	27.234671350355207	24.180695194397185	21.727301459511985	26.857331995735624
40	27.013548543486177	24.484102826488787	21.045841120646706	27.456507509378337
41	27.07486965556063	22.91344764265293	22.425828728021546	27.585853973764895
42	28.443695912480816	22.758284425388002	23.91795036307391	24.880069299057272
43	25.157433932413642	23.55031555118055	24.471545728492938	26.82070478791287
44	25.884926362786597	23.403690041682665	24.095147606527316	26.61623598900342
45	26.811445555391973	22.3870858025147	26.23548109121266	24.565987550880667
46	26.564253578328284	23.73623055327916	23.8116395638537	25.88787630453886
47	31.512333445387146	20.170104377290205	21.45955944454758	26.85800273277507
48	24.876178910444306	24.260415256051267	23.04155618362827	27.821849649876157
49	27.80189795261577	23.53523069712856	20.78059939355164	27.882271956704024
50	26.2765580792019	27.169007715067828	22.87804302348777	23.676391182242497
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	856.0
1	1045.5
2	2061.0
3	2809.5
4	2616.0
5	1880.0
6	1133.5
7	1085.0
8	1188.0
9	1206.0
10	1150.5
11	1065.5
12	1023.0
13	1087.5
14	1169.5
15	1319.5
16	1538.5
17	2045.0
18	2558.5
19	3327.5
20	4053.5
21	4758.0
22	5635.5
23	7262.5
24	8851.0
25	9973.0
26	11606.0
27	14091.0
28	16513.0
29	18588.0
30	21071.0
31	24886.5
32	29076.0
33	33181.0
34	37589.5
35	41829.5
36	47048.5
37	53226.5
38	64583.0
39	83361.5
40	99014.5
41	112394.5
42	135749.0
43	168808.0
44	173988.5
45	147232.0
46	134783.0
47	134281.0
48	140103.0
49	155998.5
50	180985.0
51	200462.0
52	203615.0
53	205196.0
54	202691.5
55	193559.0
56	188642.0
57	195521.0
58	210086.5
59	212702.0
60	199696.5
61	186203.5
62	176799.0
63	169735.5
64	171286.5
65	175385.5
66	158138.0
67	134218.0
68	115946.5
69	102509.0
70	91334.0
71	83563.0
72	81450.5
73	72013.0
74	58143.5
75	50320.0
76	45996.0
77	35032.0
78	26218.0
79	24292.5
80	21658.0
81	15005.0
82	9838.0
83	8609.0
84	7322.0
85	5848.0
86	4443.5
87	2700.0
88	1291.0
89	858.5
90	602.0
91	367.0
92	221.0
93	177.0
94	126.5
95	75.0
96	63.5
97	63.0
98	55.5
99	47.5
100	47.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	14413.0
25	13306.0
26	18024.0
27	36192.0
28	53412.0
29	56529.0
30	11304.0
31	10258.0
32	11380.0
33	10550.0
34	11750.0
35	10636.0
36	10051.0
37	9980.0
38	9523.0
39	10898.0
40	12145.0
41	14582.0
42	18370.0
43	18737.0
44	17071.0
45	21317.0
46	39036.0
47	102518.0
48	477659.0
49	854054.0
50	957477.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.378723832120414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	43.84693537105006	5.86616039214111
2	10.206050958782264	2.730878743881914
3	6.864291632151367	2.755063861490648
4	5.630384947867306	3.013094611441775
5	4.566161125411848	3.0544704334924635
6	3.958522557292906	3.1775988046344508
7	3.269968167453194	3.0623600736526817
8	2.7665063352006496	2.9609859390768833
9	2.3704414598230947	2.854213348606339
>10	15.47874962465253	35.896351190473155
>50	0.6144796779974551	5.5990217458253095
>100	0.3577979980900158	9.45544962740326
>500	0.034155171067193915	3.1868698416866326
>1k	0.02939584395127345	7.884866953313292
>5k	0.0036394854415862372	3.3753815172253154
>10k+	0.0025196437672520104	5.127232915654735
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	33147	1.1707872216876969	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	22178	0.7833504993691658	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	14597	0.5155815330188346	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	12630	0.44610500527696656	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	12152	0.42922153793552636	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	10903	0.3851055322672024	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	10489	0.3704826128543232	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	10401	0.3673743594525518	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	10393	0.3670917909614817	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	9696	0.34247301117699663	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	9019	0.318560652620187	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA	8622	0.3045381912508318	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT	8296	0.293023525239724	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	6804	0.24032450165514496	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	6696	0.2365098270256982	Illumina Single End Adapter 1 (100% over 32bp)
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	6314	0.2230171815770995	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	6226	0.2199089281753281	Illumina Single End Adapter 1 (100% over 32bp)
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	6177	0.21817819616752354	No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC	5744	0.2028841765883528	No Hit
CTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAATCCT	5601	0.19783326481047422	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG	5474	0.19334749001473595	No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC	5449	0.1924644634801418	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	4868	0.17194292681617365	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	4866	0.17187228469340612	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG	4755	0.16795164687980807	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	4330	0.15294019579170745	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA	4153	0.14668836792678083	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	3789	0.13383150158308998	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAAT	3779	0.13347829096925232	No Hit
CTGTGCGTCTGCTCCTCCTTCTCGCGCTTCTCCTGCCGCCCTAATCCT	3519	0.12429481500947312	No Hit
CTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAATCCTG	3497	0.12351775165903026	No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT	3477	0.12281133043135492	No Hit
CAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG	3451	0.121892982835377	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	3283	0.11595904452290429	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT	3222	0.11380445977849456	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	3179	0.11228565413899261	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC	3151	0.11129666442024717	No Hit
ACTACTTCTCCCCCGGACTCCTTGGTAGTCTGTTAGTGGGAGATCCTTG	3147	0.11115538017471209	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	3019	0.10663428431759003	Illumina Single End Adapter 1 (100% over 32bp)
TCTGGAAGAGGTCTTGCGAAAGCCGCCTCGAGCGCGCTCCGCGGCTGC	2988	0.10553933141469328	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC	2986	0.10546868929192574	No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCT	2948	0.10412648895934264	No Hit
CTGTGCGTCTGCTCCTCCTTCTCGCGCTTCTCCTGCCGCCCTAATCCTG	2866	0.1012301619258738	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.16152321370796263	0.0	0.0	0.0	0.0
2	0.23926486981363196	0.0	0.0	0.0	0.0
3	0.2918932512754435	0.0	0.0	0.0	0.0
4	0.3653963800150609	3.5321061383766156E-5	0.0	0.0	0.0
5	0.44133666199015814	3.5321061383766156E-5	0.0	0.0	0.0
6	0.5370920594015481	3.5321061383766156E-5	0.0	0.0	0.0
7	0.6106305092025494	3.5321061383766156E-5	0.0	0.0	0.0
8	0.6775285994634024	3.5321061383766156E-5	0.0	0.0	0.0
9	0.728355606794642	3.5321061383766156E-5	0.0	0.0	0.0
10	0.7749794078212132	3.5321061383766156E-5	0.0	0.0	0.0
11	0.8240050410218807	3.5321061383766156E-5	0.0	0.0	0.0
12	0.862928850666791	3.5321061383766156E-5	0.0	0.0	0.0
13	0.8974728487001143	3.5321061383766156E-5	0.0	0.0	0.0
14	0.9374209691251538	3.5321061383766156E-5	0.0	0.0	0.0
15	0.9759915681562265	3.5321061383766156E-5	0.0	0.0	0.0
16	1.0048488753067635	3.5321061383766156E-5	0.0	0.0	0.0
17	1.0462098381871536	3.5321061383766156E-5	0.0	0.0	0.0
18	1.0722414604269892	3.5321061383766156E-5	0.0	0.0	0.0
19	1.1102822435373054	3.5321061383766156E-5	0.0	0.0	0.0
20	1.1290024060707016	3.5321061383766156E-5	0.0	0.0	0.0
21	1.1519964170315333	3.5321061383766156E-5	0.0	0.0	0.0
22	1.1687386001274385	3.5321061383766156E-5	0.0	0.0	0.0
23	1.1867876624945428	3.5321061383766156E-5	0.0	0.0	0.0
24	1.207309199158511	3.5321061383766156E-5	0.0	0.0	0.0
25	1.2221793660010767	3.5321061383766156E-5	0.0	0.0	0.0
26	1.2453146612074435	3.5321061383766156E-5	0.0	0.0	0.0
27	1.25944308576095	3.5321061383766156E-5	0.0	0.0	0.0
28	1.2742426104807478	3.5321061383766156E-5	0.0	0.0	0.0
29	1.290525619778664	3.5321061383766156E-5	0.0	0.0	0.0
30	1.302322854280842	3.5321061383766156E-5	0.0	0.0	0.0
31	1.3143320151513225	3.5321061383766156E-5	0.0	0.0	0.0
32	1.3253521863030575	3.5321061383766156E-5	0.0	0.0	0.0
33	1.3361251100251061	3.5321061383766156E-5	0.0	0.0	0.0
34	1.348381518325273	3.5321061383766156E-5	0.0	0.0	0.0
35	1.3596489369066944	3.5321061383766156E-5	0.0	0.0	0.0
36	1.3708810344267321	3.5321061383766156E-5	0.0	0.0	0.0
37	1.382536984683375	3.5321061383766156E-5	0.0	0.0	0.0
38	1.392356239748062	3.5321061383766156E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	4070	0.0	106.21454	44
ATTAGTC	555	0.0	92.339	44
AATGAAG	2025	0.0	65.204605	44
CGACGGG	2765	0.0	65.19815	44
TTAAGCC	3975	0.0	58.15849	43
CAATGAA	4000	0.0	55.390213	43
AATCCCG	890	0.0	49.7169	43
AGGTCCC	2150	0.0	49.065067	43
TATTAGT	570	0.0	48.37696	43
AATCCTG	1910	0.0	46.5009	43
ATTAAGC	3985	0.0	45.913265	42
TAATCCT	3740	0.0	45.808414	42
GCAATGA	4025	0.0	43.822292	42
CTAATCC	5860	0.0	43.727867	41
TCGTGCG	1140	0.0	43.032993	43
AGATTAA	4065	0.0	42.783234	40
GATTAAG	4195	0.0	42.510426	41
TGTGTTA	1925	0.0	42.461956	42
ATAAGAT	1210	0.0	41.60344	43
AAGATTA	4195	0.0	41.292812	39
>>END_MODULE