FastQCFastQC Report
Tue 24 May 2016
ERR841140.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841140.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836732
Sequences flagged as poor quality0
Sequence length24-50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC87921.0507546024294516No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA84401.0086861743067075No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG70690.8448344272718147No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC54600.6525386862221118No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC54350.6495508717247578No Hit
CAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG52770.6306678841014806No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC50870.6079604939215901No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT48640.5813091886051925No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC41080.4909576782052079No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT40750.4870137630687006No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA36220.4328745643766463No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG35580.4252257592634201No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA34290.40980863645707344Illumina Single End Adapter 1 (100% over 32bp)
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA33260.39749884072797503Illumina Single End Adapter 1 (100% over 32bp)
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA27120.324118116672961No Hit
CACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA25630.30631074226873123No Hit
ATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGG24430.29196923268143204No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCA23240.27774723567402704No Hit
CGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCG22310.26663256574387023No Hit
AAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATG22230.26567646510471693No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG19400.23185440499466975No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT19190.22934464081689238No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGC18500.2210982728041954No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC18020.2153616689692757No Hit
AAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTA17870.21356898027086332No Hit
CGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATC17490.20902750223488523No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA16190.1934908668486445Illumina Single End Adapter 1 (100% over 32bp)
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA15820.18906890139256058No Hit
AAGCAGTGGTATCAACGCAGAGTG15770.18847133849308978No Hit
AAGCAGTGGTATCAACGCAGAGTGCAG15650.18703718753435986No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG15540.1857225491555241No Hit
ATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAG15410.18416888561690004No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTA15300.18285424723806426No Hit
AACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGG14260.17042493892907168No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT14160.16922981313013008No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG14050.16791517475129433No Hit
ACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATG13270.15859319351954987No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC13230.15811514319997322No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG13160.1572785551407141No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC12700.15178097646558278No Hit
TGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGAC12650.15118341356611195No Hit
AGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGT12170.1454468097311923No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA11890.14210045749415584Illumina Single End Adapter 1 (100% over 32bp)
TGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG11770.14066630653542592No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA11580.13839556751743687Illumina Single End Adapter 1 (100% over 31bp)
ATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAG11440.13672239139891865No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA11290.13492970270050628Illumina Single End Adapter 1 (100% over 32bp)
TTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTC11280.1348101901206121No Hit
CGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAA11110.13277847626241138No Hit
ATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAAT11090.13253945110262305No Hit
CTTCCGCCACGGCCGTCTCTGGAGAGCAGCAGCCATGGCCCTACGCTAC10830.1294321240253749No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC10750.12847602338622163No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGGTTGTGAGGGTTCCTGCTT10180.12166380633225453No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG9200.1099515735026269No Hit
AAGCAGTGGTATCAACGCAGAGTGCAGTGCT8970.10720278416506122No Hit
AGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGT8850.1057686332063313No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCT8650.10337838160844809No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG8620.10301984386876563Illumina Single End Adapter 1 (100% over 32bp)
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGC8590.10266130612908315No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT8510.10170520548992987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGTC7150.085.2947644
TAAGCCA11300.071.1418744
AGAACCG2800.070.9520444
TATTAGT7550.055.6050143
TAGAACC2600.051.4306743
TATATTA3400.050.2779244
TTAAGCC11200.049.14534443
CGTCAAT358.4661224E-847.09583737
ATATTAG7550.046.02298442
CATATTA7350.045.07526441
CAATGAA6450.044.8384243
CGACGGG17550.043.9636644
GCATATT7500.043.38010440
CCCCCTT9350.042.9894744
ATAGAAC2600.042.8856542
AAGCATA7600.041.93626438
TAAGCAT7700.041.89694637
TTACGCT300.001051853241.4634943
CTAATCC5250.041.44233341
ATTAAGC11300.040.84684442
Produced by FastQC (version 0.11.5)