FastQCFastQC Report
Tue 24 May 2016
ERR841139.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841139.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545717
Sequences flagged as poor quality0
Sequence length24-50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA41260.7560695378740263No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG30570.5601804598354092No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG28810.5279293113463572No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC27510.5041074403033075No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG25190.461594562749557No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC20670.3787677495844916No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC20550.37656880764205625No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG18880.34596686560983075No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT18070.331124007498392No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA16900.3096843235596472No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA16830.3084016074265599No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC15870.290810071887077No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA15660.28696192348781513No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC15550.28494622670724934No Hit
CCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCAT15000.2748677428044206No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC14860.27230231053824605No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG14770.27065310408141946No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT12620.23125539427945255No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA12590.23070565879384372No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA11320.20743352323640274Illumina Single End Adapter 1 (100% over 32bp)
CAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG10970.20101994257096628No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCGC10720.19643881352422593No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC10660.19533934255300825No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC10560.19350689093431211No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA10220.18727655543074523Illumina Single End Adapter 1 (100% over 32bp)
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC9940.18214569089839605No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG9670.1771980715279165No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC9570.17536561990922034No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC8860.16235521341647777No Hit
CTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAATCCTG8780.16088925212152086No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT8490.15557514242730205No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCC8450.1548421617798236No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG8120.14879507143812637No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC7960.14586314884821253No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGG7870.144213942391386No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT7740.14183175528708103No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGATG7240.13266949719360036No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT6790.12442346490946772No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT6640.12167478748142352No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC6600.12094180683394506No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG6410.11746014875842241No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCTG6270.11489471649224782No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG6130.11232928422607322No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA6070.11122981325485554Illumina Single End Adapter 1 (100% over 31bp)
TCTGGAAGAGGTCTTGCGAAAGCCGCCTCGAGCGCGCTCCGCGGCTGC5940.10884762615055055No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTGC5470.10023510354267873No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA5460.1000518583808091Illumina Single End Adapter 1 (100% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCA5700.069.04644
ATTAGTC2000.063.25106444
CGCTACC1050.062.4701944
CTATCAT2150.061.017444
CGTGCGC1550.060.4550244
AATGAAG4100.058.28011344
ACGGATT150.00464954858.11105343
AATCCGC250.00529643356.22316744
ACGCTTA250.00529643356.22316744
ATCCCGC2100.051.31479644
CGACGAA301.0998847E-649.64026342
TACGCTA950.049.64026342
CGACGGG4300.049.03183744
AATCCCG5650.048.34016843
AATCCTG4850.045.5303143
GGTCCCA3400.045.47462544
CAATGAA5450.045.3159643
TTGGACG1350.045.1173644
TTAAGCC5650.043.71185743
GTCATGA555.3691474E-542.59330744
Produced by FastQC (version 0.11.5)