##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841139.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	545717
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.22568107645538	35.0	33.0	39.0	25.0	39.0
2	35.401896953915674	35.0	35.0	39.0	32.0	39.0
3	35.84042461568909	35.0	35.0	39.0	33.0	39.0
4	35.908302288548825	35.0	35.0	39.0	33.0	39.0
5	36.590177692833464	35.0	35.0	40.0	33.0	41.0
6	36.60040277286579	35.0	35.0	40.0	33.0	41.0
7	36.48740464379889	35.0	35.0	40.0	33.0	41.0
8	36.477771445639405	35.0	35.0	40.0	33.0	41.0
9	36.42114502571846	35.0	35.0	40.0	33.0	41.0
10	36.365438496510095	35.0	35.0	40.0	33.0	41.0
11	36.318789775652945	35.0	35.0	40.0	33.0	41.0
12	36.23802447055892	35.0	35.0	40.0	33.0	41.0
13	36.19148569679889	35.0	35.0	40.0	33.0	41.0
14	36.149878050344775	35.0	35.0	39.0	33.0	41.0
15	36.08265639516453	35.0	35.0	39.0	33.0	41.0
16	36.01655803282654	35.0	35.0	39.0	33.0	41.0
17	35.92434906737375	35.0	35.0	39.0	33.0	41.0
18	35.82876839094256	35.0	35.0	39.0	33.0	41.0
19	35.62343852216442	35.0	35.0	38.0	33.0	40.0
20	35.54859753315363	35.0	35.0	37.0	33.0	40.0
21	35.272377441054616	35.0	35.0	37.0	33.0	40.0
22	35.08759301982529	35.0	34.0	37.0	32.0	39.0
23	34.986639595248086	35.0	34.0	37.0	32.0	39.0
24	34.868626046100815	35.0	34.0	36.0	32.0	39.0
25	34.725643141907575	35.0	34.0	36.0	32.0	39.0
26	34.65356703840287	35.0	34.0	36.0	31.0	39.0
27	34.604363036205264	35.0	34.0	36.0	32.0	38.0
28	34.69563738333214	35.0	34.0	35.0	32.0	38.0
29	34.71825088541072	35.0	35.0	35.0	33.0	37.0
30	34.709614111645365	35.0	35.0	35.0	33.0	37.0
31	34.65481976311389	35.0	35.0	35.0	33.0	37.0
32	34.672797756193354	35.0	35.0	35.0	33.0	37.0
33	34.64920391453693	35.0	35.0	35.0	33.0	36.0
34	34.656263121891406	35.0	35.0	35.0	33.0	36.0
35	34.58378674840018	35.0	35.0	35.0	33.0	36.0
36	34.520935725533235	35.0	35.0	35.0	33.0	36.0
37	34.53106978099742	35.0	35.0	35.0	33.0	36.0
38	34.50131310885282	35.0	35.0	35.0	33.0	36.0
39	34.45119508693187	35.0	35.0	35.0	33.0	36.0
40	34.38263254519493	35.0	35.0	35.0	33.0	36.0
41	34.3368173498748	35.0	35.0	35.0	33.0	35.0
42	34.334186406744585	35.0	35.0	35.0	33.0	35.0
43	34.32689438619709	35.0	35.0	35.0	33.0	35.0
44	34.30997087707976	35.0	35.0	35.0	33.0	35.0
45	34.263794592232706	35.0	35.0	35.0	33.0	35.0
46	34.25791891218244	35.0	35.0	35.0	33.0	35.0
47	34.283120774154725	35.0	35.0	35.0	33.0	35.0
48	34.252209800117036	35.0	34.0	35.0	33.0	35.0
49	34.210346255649384	35.0	34.0	35.0	33.0	35.0
50	34.11929596912379	35.0	34.0	35.0	32.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	16.0
14	31.0
15	49.0
16	15.0
17	0.0
18	0.0
19	0.0
20	5.0
21	17.0
22	43.0
23	112.0
24	288.0
25	582.0
26	1149.0
27	2087.0
28	3658.0
29	5982.0
30	10026.0
31	16039.0
32	27362.0
33	53857.0
34	184393.0
35	57452.0
36	63232.0
37	69314.0
38	21856.0
39	19406.0
40	8746.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	41.52793480870121	23.31226624789039	35.1597989434084
2	30.92738543970593	20.60280328448628	26.274424289512694	22.195386986295095
3	31.187043834075173	16.458713948804967	22.687949981400614	29.666292235719244
4	22.305334083416863	19.487206739024074	27.707951190818687	30.499507986740383
5	26.889395052747123	21.620546913510115	27.36308379618007	24.126974237562692
6	28.242294082830476	24.821106910724787	22.403186999855237	24.533412006589497
7	30.884872562152175	23.77605975258238	21.502720274427954	23.836347410837487
8	30.943694259112327	20.38419180637583	23.864933656089146	24.8071802784227
9	25.883928849568548	24.383700709342023	24.223361192706108	25.509009248383318
10	30.13228468235367	20.68508036216574	24.243884650835508	24.938750304645083
11	31.85992006846039	19.187234409043516	25.137388060111743	23.815457462384348
12	27.607532842114136	18.924827337246228	24.801133188081	28.666506632558637
13	24.653620832775964	19.643881352422593	28.272529534538965	27.42996828026248
14	24.02875483080058	22.7989965494936	23.546820055083494	29.625428564622325
15	24.187261895817798	23.190224970085225	25.718275223238418	26.90423791085856
16	26.750128729726214	26.518873335446763	23.926870520801074	22.804127414025952
17	27.157116234238625	23.258392170300723	21.287590454393026	28.29690114106762
18	27.404497202762606	22.95200625965473	20.950785846876677	28.692710690705987
19	25.929190404550344	21.801043397951688	26.16319447625784	26.10657172124013
20	29.358623608940164	18.708598046240084	22.823368156022262	29.10941018879749
21	28.148289314791363	21.18314071212735	22.366904457805052	28.30166551527623
22	26.830390110625103	21.702274255703962	25.8540598881838	25.61327574548713
23	28.091666559773653	20.43751614847989	22.721667091184624	28.74915020056183
24	29.232734182735744	21.501437558294867	22.484547851725345	26.781280407244047
25	26.531860487395083	21.145938497137088	26.220698447321684	26.101502568146145
26	28.942477155255403	20.304238681299154	24.315583300010367	26.437700863435083
27	26.300602800152006	22.309380285678092	24.424582920413986	26.965433993755916
28	26.417399345194013	20.40066457920634	24.962880544894805	28.21905553070484
29	30.864465516584787	21.136943524125062	24.494649453520697	23.50394150576945
30	25.585607270906085	23.51960306629732	23.527561928684708	27.367227734111875
31	26.064876704640678	21.496887898074277	28.456732624259974	23.981502773025074
32	27.0670912270133	20.79237356094775	23.72954619245258	28.41098901958637
33	27.360908442806952	21.243217021911747	23.891401648536174	27.504472886745134
34	23.711960068051486	23.64729932993339	27.289788531787256	25.350952070227873
35	26.302033833871885	23.3932474814674	23.72528986884623	26.57942881581448
36	25.592681840594235	23.030093365780726	24.846312290245105	26.530912503379938
37	24.71868618413304	23.649923246292566	23.325541517866945	28.305849051707444
38	27.822482907549524	23.53599881409544	22.338876235233784	26.302642043121256
39	26.20765515216596	25.214464111195568	22.189645395205172	26.3882353414333
40	25.391561459244283	25.672496076811687	21.32113997653156	27.614802487412472
41	26.03414620288867	24.50458786336649	21.467588703426387	27.993677230318454
42	27.818356588767596	23.504417410044635	23.633194759925143	25.044031241262633
43	24.28805109510051	24.496015387713665	24.3969652009157	26.818968316270126
44	26.036469838748378	23.95256769837921	23.310332845193475	26.700629617678935
45	25.717793198156492	22.791481570104345	27.83616483390726	23.6545603978319
46	26.13748944878129	24.883910412560507	23.743226798315156	25.235373340343042
47	30.62335389355161	22.069851063784704	21.088808700977015	26.21798634168667
48	24.50667229841211	24.545610538403665	22.52918168098802	28.418535482196205
49	27.650806798041977	23.386210994472155	21.254556164616126	27.70842604286975
50	27.534061985327146	24.658132454958327	22.84024554574038	24.967560013974147
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	816.0
1	784.0
2	865.0
3	917.0
4	837.5
5	706.5
6	538.0
7	452.5
8	410.0
9	382.0
10	369.0
11	392.5
12	412.0
13	374.0
14	338.0
15	398.5
16	454.5
17	494.0
18	549.5
19	726.0
20	920.0
21	1222.5
22	1563.0
23	1850.5
24	2101.0
25	2507.5
26	3142.0
27	3706.5
28	4077.5
29	4530.5
30	5099.0
31	6081.5
32	7298.0
33	8173.0
34	9167.0
35	10533.0
36	11763.5
37	12863.5
38	15064.5
39	19196.5
40	22376.5
41	23729.5
42	25925.0
43	30900.5
44	33049.0
45	28722.5
46	26252.0
47	26699.0
48	27048.0
49	29246.5
50	34712.0
51	38574.0
52	38096.5
53	37220.5
54	37513.5
55	37048.0
56	35613.5
57	35805.0
58	38711.0
59	40096.0
60	37602.0
61	33561.5
62	30160.5
63	28773.0
64	30715.0
65	33258.0
66	29602.0
67	23228.0
68	19660.0
69	17594.5
70	15559.5
71	14138.5
72	14093.5
73	13392.0
74	10404.0
75	7844.0
76	6895.5
77	5045.5
78	3398.5
79	3391.5
80	3417.5
81	2437.5
82	1535.0
83	1363.5
84	1163.0
85	846.0
86	598.5
87	358.5
88	150.0
89	114.5
90	79.5
91	41.5
92	18.5
93	10.5
94	7.0
95	3.0
96	2.5
97	2.0
98	6.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	2913.0
25	2636.0
26	3340.0
27	4762.0
28	6886.0
29	10031.0
30	1991.0
31	1884.0
32	2095.0
33	1916.0
34	2207.0
35	2088.0
36	2015.0
37	1756.0
38	1911.0
39	2153.0
40	2311.0
41	2829.0
42	3371.0
43	3154.0
44	2863.0
45	3172.0
46	5486.0
47	17381.0
48	65807.0
49	148315.0
50	240444.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.90692134710555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	53.943738445705804	10.738537584074992
2	9.831535595577492	3.9143121164485937
3	6.2621327138351575	3.739793501983593
4	4.606467689268884	3.6680235991303487
5	4.039646465101527	4.020846222544453
6	3.2970755274498957	3.938077392024698
7	2.6921216321351573	3.7514297511429193
8	2.197419273466645	3.4995082114811455
9	1.8506566881429845	3.315678941822152
>10	10.711947657422849	35.51655634162238
>50	0.3269744754602743	4.392017799567386
>100	0.19222706914667423	7.648233609881312
>500	0.02449952842065456	3.4794370105140278
>1k	0.023557238866013998	8.377547917761982
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA	4126	0.7560695378740263	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCG	3057	0.5601804598354092	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAAG	2881	0.5279293113463572	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGC	2751	0.5041074403033075	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCG	2519	0.461594562749557	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	2067	0.3787677495844916	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGAC	2055	0.37656880764205625	No Hit
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG	1888	0.34596686560983075	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	1807	0.331124007498392	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCCA	1690	0.3096843235596472	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	1683	0.3084016074265599	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTC	1587	0.290810071887077	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	1566	0.28696192348781513	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCC	1555	0.28494622670724934	No Hit
CCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCAT	1500	0.2748677428044206	No Hit
TCGCTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATC	1486	0.27230231053824605	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTG	1477	0.27065310408141946	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	1262	0.23125539427945255	No Hit
CAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGA	1259	0.23070565879384372	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	1132	0.20743352323640274	Illumina Single End Adapter 1 (100% over 32bp)
CAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGG	1097	0.20101994257096628	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCCGC	1072	0.19643881352422593	No Hit
CAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGC	1066	0.19533934255300825	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC	1056	0.19350689093431211	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	1022	0.18727655543074523	Illumina Single End Adapter 1 (100% over 32bp)
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGC	994	0.18214569089839605	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCG	967	0.1771980715279165	No Hit
AACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGC	957	0.17536561990922034	No Hit
ATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGC	886	0.16235521341647777	No Hit
CTGCGCGTCTGCTCCTCCTTCTCACGTTTCTCCTGCCGCCCTAATCCTG	878	0.16088925212152086	No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT	849	0.15557514242730205	No Hit
CCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCC	845	0.1548421617798236	No Hit
CATCCTGCTTCGTCAGGTTTATACCACTTTATTTGGTGTGCTGTGTTAG	812	0.14879507143812637	No Hit
CTGCGCATCAGCTCCTCCTTCTCACGTTTCTCCTGCCGTCCTAATCCC	796	0.14586314884821253	No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGG	787	0.144213942391386	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGAT	774	0.14183175528708103	No Hit
AGGCCTGGTTTGCGCTGGAGAGTGGTCTTGGTTGTGAGGGTCATAAGATG	724	0.13266949719360036	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT	679	0.12442346490946772	No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTT	664	0.12167478748142352	No Hit
ATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGCAGGTCCC	660	0.12094180683394506	No Hit
ACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG	641	0.11746014875842241	No Hit
CTCTGCAGGTACCATGGAGCTGAGCTATAGGCTCTTCATCTGCCTCCTG	627	0.11489471649224782	No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG	613	0.11232928422607322	No Hit
TCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	607	0.11122981325485554	Illumina Single End Adapter 1 (100% over 31bp)
TCTGGAAGAGGTCTTGCGAAAGCCGCCTCGAGCGCGCTCCGCGGCTGC	594	0.10884762615055055	No Hit
CTGCGCGTCTGCTCCTCCTTCTCGCGCTTCTGCTGCCGCCCTAATCCTGC	547	0.10023510354267873	No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	546	0.1000518583808091	Illumina Single End Adapter 1 (100% over 32bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.15520865210356283	0.0	0.0	0.0	0.0
2	0.26790442665337527	0.0	0.0	0.0	0.0
3	0.3289250655559567	0.0	0.0	0.0	0.0
4	0.40991942710232593	0.0	0.0	0.0	0.0
5	0.4945786918860875	0.0	0.0	0.0	0.0
6	0.5968294922093319	0.0	0.0	0.0	0.0
7	0.6626145053205232	0.0	0.0	0.0	0.0
8	0.7234518990612351	0.0	0.0	0.0	0.0
9	0.771828621794813	0.0	0.0	0.0	0.0
10	0.8137917638629546	0.0	0.0	0.0	0.0
11	0.8612522607871845	0.0	0.0	0.0	0.0
12	0.8982677834848466	0.0	0.0	0.0	0.0
13	0.9352833061825085	0.0	0.0	0.0	0.0
14	0.9679009449952998	0.0	0.0	0.0	0.0
15	1.0049164676929618	0.0	0.0	0.0	0.0
16	1.041382254905015	0.0	0.0	0.0	0.0
17	1.075099364689024	0.0	0.0	0.0	0.0
18	1.103502364778814	0.0	0.0	0.0	0.0
19	1.1330048358398217	0.0	0.0	0.0	0.0
20	1.155177500426045	0.0	0.0	0.0	0.0
21	1.1819312940590085	0.0	0.0	0.0	0.0
22	1.2020882618646662	0.0	0.0	0.0	0.0
23	1.2200462877278884	0.0	0.0	0.0	0.0
24	1.2378210684292408	0.0	0.0	0.0	0.0
25	1.251014720083853	0.0	0.0	0.0	0.0
26	1.271904668536989	0.0	0.0	0.0	0.0
27	1.288030242781515	0.0	0.0	0.0	0.0
28	1.305988268644737	0.0	0.0	0.0	0.0
29	1.3191819202993493	0.0	0.0	0.0	0.0
30	1.3360404751913537	0.0	0.0	0.0	0.0
31	1.3494173720078355	0.0	0.0	0.0	0.0
32	1.3618780430149693	0.0	0.0	0.0	0.0
33	1.3769041462882776	0.0	0.0	0.0	0.0
34	1.3944956818277605	0.0	0.0	0.0	0.0
35	1.408055823806112	0.0	0.0	0.0	0.0
36	1.424181398050638	0.0	0.0	0.0	0.0
37	1.4368253142196412	0.0	0.0	0.0	0.0
38	1.449835720712384	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCCA	570	0.0	69.046	44
ATTAGTC	200	0.0	63.251064	44
CGCTACC	105	0.0	62.47019	44
CTATCAT	215	0.0	61.0174	44
CGTGCGC	155	0.0	60.45502	44
AATGAAG	410	0.0	58.280113	44
ACGGATT	15	0.004649548	58.111053	43
AATCCGC	25	0.005296433	56.223167	44
ACGCTTA	25	0.005296433	56.223167	44
ATCCCGC	210	0.0	51.314796	44
CGACGAA	30	1.0998847E-6	49.640263	42
TACGCTA	95	0.0	49.640263	42
CGACGGG	430	0.0	49.031837	44
AATCCCG	565	0.0	48.340168	43
AATCCTG	485	0.0	45.53031	43
GGTCCCA	340	0.0	45.474625	44
CAATGAA	545	0.0	45.31596	43
TTGGACG	135	0.0	45.11736	44
TTAAGCC	565	0.0	43.711857	43
GTCATGA	55	5.3691474E-5	42.593307	44
>>END_MODULE