FastQCFastQC Report
Tue 24 May 2016
ERR841137.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841137.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4531015
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA418080.922707163847394No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC247860.5470297494049347No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT217520.4800690353044516No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA136410.30105837213074776No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC117820.2600300374198717No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG102340.22586550695594698No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT100850.2225770605482436No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC89240.19695366270030004No Hit
ACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCA67370.14868633187045285No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA66700.1472076344924923No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT62960.1389534133080557No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC60530.1335903765491838No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA59580.13149371608789642No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG56820.12540236569510363No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT53310.11765575704339977No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT50750.11200580885298327No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT50390.11121128488870595No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCAT49400.10902634398694332No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTA48140.10624551011197271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAC950.0204.263544
CGATTTA900.0192.9155444
GCGATAC1300.0188.5509244
GATGGTA2300.0177.6204444
ATAGTCC1700.0174.2247544
CCGGACA650.0172.8383544
TAGCTTG1200.0170.2195744
TAGCCAC600.0153.1976244
CGCACCA950.0150.5099544
CGGAAAT900.0147.5236544
TTAACTC2100.0141.0390844
CTATCAT72400.0136.2697144
CTAAGTC150.008554722136.1756644
CCACGCG1100.0129.9858644
TCCGAAT1200.0119.153744
ACGAACA1250.0114.3875644
GGCGCTA651.03682396E-10109.9880444
GCGCGTA950.0108.6791743
CGAGTGA650.0108.6791643
CCCACCG1700.0108.139544
Produced by FastQC (version 0.11.5)