##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841137.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4531015
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.55705487622531	39.0	38.0	39.0	35.0	39.0
2	37.96930577365116	39.0	38.0	39.0	35.0	39.0
3	38.255247223856024	39.0	38.0	39.0	35.0	40.0
4	38.79657692592057	39.0	39.0	41.0	35.0	41.0
5	39.47511319207727	41.0	39.0	41.0	36.0	41.0
6	39.499295191033355	41.0	39.0	41.0	36.0	41.0
7	39.46779739197509	41.0	39.0	41.0	36.0	41.0
8	39.439906290312436	41.0	39.0	41.0	36.0	41.0
9	39.38127505647189	41.0	39.0	41.0	36.0	41.0
10	39.36930930486878	41.0	39.0	41.0	35.0	41.0
11	39.2844243508353	40.0	39.0	41.0	35.0	41.0
12	39.22198491949376	40.0	39.0	41.0	35.0	41.0
13	39.090049801203484	40.0	38.0	41.0	35.0	41.0
14	39.1341129967568	40.0	38.0	41.0	35.0	41.0
15	39.131666304349025	40.0	38.0	41.0	35.0	41.0
16	39.18673255330207	40.0	39.0	41.0	35.0	41.0
17	39.23644150372488	40.0	39.0	41.0	35.0	41.0
18	39.19310286988677	41.0	39.0	41.0	35.0	41.0
19	39.13285411767562	41.0	39.0	41.0	35.0	41.0
20	39.13615514404609	41.0	39.0	41.0	35.0	41.0
21	39.165892851822385	41.0	39.0	41.0	35.0	41.0
22	39.184299544362574	41.0	39.0	41.0	35.0	41.0
23	39.14180796135082	41.0	39.0	41.0	35.0	41.0
24	39.055050799875964	40.0	38.0	41.0	35.0	41.0
25	39.072874622997595	40.0	38.0	41.0	35.0	41.0
26	39.02259172857497	40.0	38.0	41.0	35.0	41.0
27	39.01345563758805	40.0	38.0	41.0	35.0	41.0
28	38.97376334485113	40.0	38.0	41.0	35.0	41.0
29	38.93160547058977	40.0	38.0	41.0	35.0	41.0
30	38.904798818367986	40.0	38.0	41.0	35.0	41.0
31	38.83531976003341	40.0	38.0	41.0	35.0	41.0
32	38.65185584072557	40.0	38.0	41.0	35.0	41.0
33	38.5410179861392	40.0	37.0	41.0	35.0	41.0
34	38.5372842329743	40.0	37.0	41.0	35.0	41.0
35	38.59372276540725	40.0	37.0	41.0	35.0	41.0
36	38.55552339779821	40.0	37.0	41.0	35.0	41.0
37	38.55068633950822	40.0	37.0	41.0	35.0	41.0
38	38.44243703407993	40.0	37.0	41.0	35.0	41.0
39	38.41332068491981	40.0	37.0	41.0	34.0	41.0
40	38.34025853201989	40.0	36.0	41.0	34.0	41.0
41	38.28557788561911	40.0	36.0	41.0	34.0	41.0
42	38.17255303722543	40.0	36.0	41.0	34.0	41.0
43	38.08924068101367	40.0	36.0	41.0	34.0	41.0
44	37.984577398834794	39.0	36.0	41.0	34.0	41.0
45	37.805714111975114	39.0	35.0	41.0	34.0	41.0
46	37.51345648367567	39.0	35.0	41.0	33.0	41.0
47	37.27910959635714	39.0	35.0	40.0	33.0	41.0
48	37.23909610449828	39.0	35.0	40.0	33.0	41.0
49	37.37160083517475	39.0	35.0	40.0	33.0	41.0
50	37.28800273162973	38.0	36.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	7.0
21	29.0
22	65.0
23	124.0
24	308.0
25	628.0
26	1058.0
27	2314.0
28	4650.0
29	9355.0
30	18097.0
31	32891.0
32	56918.0
33	102260.0
34	195504.0
35	156863.0
36	245804.0
37	402694.0
38	591814.0
39	981237.0
40	1728394.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	48.864150747680156	17.734657687074527	33.40119156524531
2	22.928549121995843	21.704739445797465	33.01725992961842	22.349451502588273
3	23.126562149981847	22.66408299244209	26.93025734851904	27.27909750905702
4	20.514608757640396	26.29823560504655	30.776327158484357	22.410828478828694
5	16.56317624196786	27.191457101775207	30.205329269490388	26.04003738676654
6	19.927676249140642	27.10573679407373	27.84106872301239	25.125518233773224
7	21.36135060245883	27.54031933242331	23.213849435501757	27.884480629616103
8	23.2476829143139	26.041957486346877	27.328909747595187	23.381449851744033
9	21.914361351705963	29.006370537285797	27.45016293258795	21.62910517842029
10	25.50947193951024	24.018878772195634	26.02531662331729	24.446332664976833
11	24.115457574075567	23.6498003206787	26.557316627731314	25.677425477514422
12	21.501451661493064	26.358575286111392	26.864378069814382	25.27559498258117
13	24.993185853500815	23.901818908125442	25.889033693333612	25.215961545040127
14	18.518455577834107	27.412158202963354	25.58665111459574	28.482735104606803
15	20.442925039974487	33.27044823290146	24.97956859555751	21.30705813156655
16	20.190531260655725	33.45440260074178	23.18125188285627	23.17381425574623
17	21.55013832441517	35.56704182175517	21.458039754889356	21.424780098940303
18	20.647581171106253	27.417035697299614	23.03393389781318	28.90144923378095
19	21.77075114516284	25.421721181677835	23.83355605752795	28.973971615631378
20	22.5164118856371	24.21887811009233	27.691543726957423	25.573166277313142
21	19.9140148509771	31.215566490069	26.160893309777165	22.70952534917673
22	24.179107771658227	25.646770094559386	24.17385508544995	26.000267048332436
23	26.197772463785707	23.976371740106796	28.666645332226885	21.15921046388061
24	21.638043573018408	28.441618489455458	26.239926374112642	23.680411563413493
25	23.10634262890698	25.974627181898306	25.183328938707927	25.735701250486787
26	22.172668923773877	31.75020428797063	26.031495996533266	20.04563079172222
27	23.325289581744237	26.73672145720146	25.292635486987265	24.645353474067036
28	23.207855592719273	24.679346313593676	26.06683245183002	26.045965641857027
29	22.333281102114732	28.440641471407464	25.257598966902517	23.968478459575287
30	23.954596975847704	28.54444337817113	26.666819063276883	20.834140582704283
31	24.998577178305727	29.474579947608166	23.515528413120677	22.01131446096543
32	21.112632138441658	30.957677317605626	24.551738255103807	23.377952288848906
33	20.88198511349732	23.61134316713698	32.40571751288711	23.100954206478594
34	22.800308590977874	28.906820027031426	26.77875477121355	21.514116610777148
35	20.548155230770515	24.63098058244325	25.73229833436062	29.088565852425614
36	19.15753479376327	28.21685341819154	26.567136547312487	26.058475240732704
37	19.691052406448946	32.86407582883783	23.677044772293133	23.767826992420094
38	25.2904036597	24.544424999909545	25.88767383997134	24.27749750041911
39	19.507558216999286	29.593829715050397	27.90048829227798	22.99812377567234
40	18.330749177277955	26.8351327839061	29.059180447821753	25.774937590994185
41	18.737458049495327	30.724599515517152	25.499116389544067	25.03882604544345
42	18.95880665081649	30.72773433890534	25.29914642737886	25.01431258289931
43	21.18719028159782	24.608135485080798	26.40927326050791	27.79540097281347
44	21.277664160522935	27.26954775974878	24.128010667638378	27.324777412089908
45	20.401115891351328	24.55298512789025	32.11684135857994	22.929057622178483
46	19.80319384576439	28.99494413231044	27.31822432863843	23.883637693286737
47	20.641838651313897	29.914663625473064	27.045238302606435	22.398259420606607
48	22.43827729010785	27.377602611821793	25.11250460498736	25.071615493082994
49	19.231999751691898	30.54100127854797	24.112328377915382	26.114670591844753
50	19.52261625432012	29.010312126886	30.926496471457725	20.540575147336153
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	35438.0
1	33630.5
2	37602.0
3	42749.5
4	38993.0
5	24692.0
6	9710.0
7	6714.5
8	7463.0
9	6554.0
10	5223.5
11	4366.0
12	4000.5
13	3911.0
14	4155.5
15	4697.5
16	5619.0
17	8291.0
18	11785.0
19	13728.0
20	15298.0
21	17451.0
22	18151.5
23	17753.0
24	18458.5
25	24416.5
26	35525.5
27	48460.0
28	59336.5
29	69172.0
30	84845.0
31	125674.5
32	188937.0
33	210574.5
34	203011.5
35	217441.5
36	226286.0
37	221593.5
38	220987.5
39	213511.0
40	209876.5
41	227499.5
42	253359.5
43	280138.5
44	303504.0
45	330219.5
46	362150.0
47	376027.5
48	403437.5
49	445114.5
50	489944.5
51	513347.5
52	459532.5
53	377846.0
54	327702.5
55	293454.0
56	264490.5
57	245375.0
58	224975.5
59	198924.5
60	165312.5
61	136039.5
62	110911.5
63	85297.0
64	66660.5
65	56109.5
66	38481.0
67	22567.5
68	19114.5
69	16851.5
70	12611.5
71	7417.0
72	3923.0
73	2748.5
74	2242.0
75	1765.5
76	1335.5
77	781.5
78	342.5
79	184.5
80	120.0
81	181.0
82	252.0
83	228.5
84	220.0
85	112.5
86	6.0
87	2.0
88	2.0
89	2.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	14134.0
25	14658.0
26	15925.0
27	19875.0
28	24635.0
29	23492.0
30	43187.0
31	76114.0
32	79468.0
33	13312.0
34	12330.0
35	13107.0
36	14764.0
37	20319.0
38	10262.0
39	12230.0
40	14492.0
41	17905.0
42	21989.0
43	40184.0
44	147610.0
45	325748.0
46	512610.0
47	523657.0
48	843670.0
49	785027.0
50	890311.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.722018592625318
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.1428115502263	9.559682456441193
2	10.77952048488102	2.742745200564836
3	4.209567927687418	1.6066260432887565
4	2.1218289360161493	1.0797578869747138
5	1.2212834364496217	0.7768595292688713
6	0.805912595666599	0.6151701015660841
7	0.5407153066821652	0.48152931294493234
8	0.4354369548849165	0.4431709628769647
9	0.3481015104659708	0.3985698499442133
>10	2.6087091041061896	6.705085427679604
>50	0.4298354250661695	3.8937390449918965
>100	1.112146949651386	37.89494719432939
>500	0.20100717226344553	15.946238800550287
>1k	0.03721067029790428	9.190850200175111
>5k	0.003477632738121895	2.732638528405086
>10k+	0.0024343429166853265	5.932389459998076
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	41808	0.922707163847394	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	24786	0.5470297494049347	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	21752	0.4800690353044516	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	13641	0.30105837213074776	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	11782	0.2600300374198717	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	10234	0.22586550695594698	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	10085	0.2225770605482436	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	8924	0.19695366270030004	No Hit
ACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATTCTCCGTGCCA	6737	0.14868633187045285	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	6670	0.1472076344924923	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	6296	0.1389534133080557	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	6053	0.1335903765491838	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA	5958	0.13149371608789642	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG	5682	0.12540236569510363	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT	5331	0.11765575704339977	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT	5075	0.11200580885298327	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	5039	0.11121128488870595	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCAT	4940	0.10902634398694332	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTA	4814	0.10624551011197271	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.03615084037461805	0.0	0.0	0.0	0.0
2	0.05603600959166986	0.0	0.0	0.0	0.0
3	0.08004828940093997	0.0	0.0	0.0	0.0
4	0.11650811131722141	0.0	0.0	0.0	0.0
5	0.14102800365922427	0.0	0.0	0.0	0.0
6	0.19774818666457736	0.0	0.0	0.0	0.0
7	0.2268586619112936	0.0	0.0	0.0	0.0
8	0.26654071990492195	0.0	0.0	0.0	0.0
9	0.3055827447051047	0.0	0.0	0.0	0.0
10	0.3338324856571872	0.0	0.0	0.0	0.0
11	0.36462028927293333	0.0	0.0	0.0	0.0
12	0.3940838862815506	0.0	0.0	0.0	0.0
13	0.4218922250312568	0.0	0.0	0.0	0.0
14	0.45272416886724054	0.0	0.0	0.0	0.0
15	0.4862045259174821	0.0	0.0	0.0	0.0
16	0.5213180711165158	0.0	0.0	0.0	0.0
17	0.5626995275892929	0.0	0.0	0.0	0.0
18	0.5934431909848015	0.0	0.0	0.0	0.0
19	0.6254007104368448	0.0	0.0	0.0	0.0
20	0.6578217022013831	0.0	0.0	0.0	0.0
21	0.6882122438349906	0.0	0.0	0.0	0.0
22	0.7127100660668746	0.0	0.0	0.0	0.0
23	0.7383334639148182	0.0	0.0	0.0	0.0
24	0.7676205000424849	0.0	0.0	0.0	0.0
25	0.7934425288814978	0.0	0.0	0.0	0.0
26	0.825179347232353	0.0	0.0	0.0	0.0
27	0.8508027450802966	0.0	0.0	0.0	0.0
28	0.8810167258329535	0.0	0.0	0.0	0.0
29	0.91105414570466	0.0	0.0	0.0	0.0
30	0.9400101301805446	0.0	0.0	0.0	0.0
31	0.9656997383588446	0.0	0.0	0.0	0.0
32	0.9931770254567686	0.0	0.0	0.0	0.0
33	1.0192197553969695	0.0	0.0	0.0	0.0
34	1.0499192785722404	0.0	0.0	0.0	0.0
35	1.075299905208877	0.0	0.0	0.0	0.0
36	1.0976789968693548	0.0	0.0	0.0	0.0
37	1.1136798267054953	0.0	0.0	0.0	0.0
38	1.1266349813452394	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAC	95	0.0	204.2635	44
CGATTTA	90	0.0	192.91554	44
GCGATAC	130	0.0	188.55092	44
GATGGTA	230	0.0	177.62044	44
ATAGTCC	170	0.0	174.22475	44
CCGGACA	65	0.0	172.83835	44
TAGCTTG	120	0.0	170.21957	44
TAGCCAC	60	0.0	153.19762	44
CGCACCA	95	0.0	150.50995	44
CGGAAAT	90	0.0	147.52365	44
TTAACTC	210	0.0	141.03908	44
CTATCAT	7240	0.0	136.26971	44
CTAAGTC	15	0.008554722	136.17566	44
CCACGCG	110	0.0	129.98586	44
TCCGAAT	120	0.0	119.1537	44
ACGAACA	125	0.0	114.38756	44
GGCGCTA	65	1.03682396E-10	109.98804	44
GCGCGTA	95	0.0	108.67917	43
CGAGTGA	65	0.0	108.67916	43
CCCACCG	170	0.0	108.1395	44
>>END_MODULE