FastQCFastQC Report
Tue 24 May 2016
ERR841136.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841136.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4222977
Sequences flagged as poor quality0
Sequence length24-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA299120.7083154845503539No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA282250.668367362644883No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA279870.6627315280192149No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA240890.5704269760408357No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA240480.5694560969666659No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA216400.5124347113422593No Hit
AAAAAAAAAAAAAAAAAAAAAAAA191600.4537083673437009No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA139830.3311171242467103No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT125940.2982256356120339No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT100220.23732073369094833No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT99890.23653929443612884No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97420.23069034001369174No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT84760.20071148860152446No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA84160.19929068995639806No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74300.17594223222148736No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG70380.16665968107332813No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTT64870.15361201351558393No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC57660.13653874979664818No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA56710.13428915194186472No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG56280.13327091291285745No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51120.12105204456477031No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA49650.1175710878842106No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC47820.11323765201657504No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA46740.1106802144553475No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA44990.10653621840706212No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43270.10246326229103309No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTT42520.10068726398462506No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC42360.10030838434592468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGGT1350.0155.1462244
CGCGTAC400.0155.1462244
TCGGTGT2400.0145.4495844
CTACGTA1200.0142.2173844
CTTCGGT1150.0141.6552444
TATAAGG500.0139.6316244
AATAGTC1450.0139.0966244
GTCGTTC254.6187124E-6124.11744
CGCTCCC750.0124.1169844
GGTCGTT700.0121.90060444
TCCGATG551.8189894E-12112.83362644
TGCACCG1100.0112.83362644
ATCCGTA1250.0111.7052944
CGACAAT750.0105.1413743
CGTATAG450.0105.14136543
TTCGATG650.0105.14136543
AGCCGGC456.8248482E-9103.4308244
TGGTCGA456.8248482E-9103.4308244
GTTCGGC2900.0103.3285843
GGCGCAT1450.0101.6475344
Produced by FastQC (version 0.11.5)