FastQCFastQC Report
Tue 24 May 2016
ERR841135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7454961
Sequences flagged as poor quality0
Sequence length24-50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA526580.7063484302600644No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA369410.495522377648924No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA354180.4750930286556831No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA340260.45642089878136183No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA282070.37836549379668116No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA277380.3720743810732209No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT273440.36678930983005814No Hit
AAAAAAAAAAAAAAAAAAAAAAAA209000.2803502258428984No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA184030.24685575149219424No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA107290.14391758722815587No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG101520.13617777477306722No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTT97220.13040980361936166No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC79350.10643918861547365No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT75330.10104680628107914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCG401.8189894E-11136.6977544
AGGTACG750.0135.3958744
TCGAATG650.0132.1912444
TGACGGC1550.0131.0282744
CCGATAA900.0130.1883244
TATGCGC550.0127.82126644
TAGTACG254.4630324E-6124.98080444
CTACCGC1200.0123.6789244
CATCTAG650.0120.1738544
CTCGCCC1300.0120.1738544
ACACCGA1050.0101.9873343
GATCGTC351.8735591E-10101.9873243
CAACGTT307.528797E-9101.9873243
CCCGTGT700.0100.43144
TCTATAC1500.098.58774643
ACGACTG403.9253973E-797.6412544
AGCGCCT5500.095.1558344
GGTGCAT950.085.8840643
CAGGCGA3200.085.4360944
GGCGTGC1250.081.5898643
Produced by FastQC (version 0.11.5)