##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841135.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7454961
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.99322974325419	39.0	37.0	39.0	33.0	39.0
2	37.591616642930795	39.0	37.0	39.0	35.0	39.0
3	37.66249387488412	39.0	37.0	39.0	35.0	39.0
4	38.074553307522336	39.0	37.0	39.0	35.0	41.0
5	38.95073870943121	40.0	38.0	41.0	35.0	41.0
6	38.91351436982702	40.0	38.0	41.0	35.0	41.0
7	38.93531783734348	40.0	38.0	41.0	35.0	41.0
8	38.858863916256574	40.0	38.0	41.0	35.0	41.0
9	38.83255928501839	40.0	38.0	41.0	35.0	41.0
10	38.85101478062729	40.0	38.0	41.0	35.0	41.0
11	38.791229491341404	40.0	38.0	41.0	35.0	41.0
12	38.69550840037929	40.0	38.0	41.0	35.0	41.0
13	38.56394674633442	40.0	38.0	41.0	34.0	41.0
14	38.57817995291994	40.0	38.0	41.0	34.0	41.0
15	38.50923660096947	40.0	38.0	41.0	35.0	41.0
16	38.46752504808543	40.0	37.0	41.0	35.0	41.0
17	38.527938777949345	40.0	37.0	41.0	35.0	41.0
18	38.510405218752986	40.0	37.0	41.0	35.0	41.0
19	38.46817213396556	40.0	37.0	41.0	35.0	41.0
20	38.54760232816778	40.0	37.0	41.0	35.0	41.0
21	38.54136420029562	40.0	37.0	41.0	35.0	41.0
22	38.52879659598487	40.0	37.0	41.0	35.0	41.0
23	38.52302607619275	40.0	37.0	41.0	35.0	41.0
24	38.409237016799956	40.0	36.0	41.0	35.0	41.0
25	38.37389814835356	40.0	37.0	41.0	34.0	41.0
26	38.37250645811131	40.0	36.0	41.0	34.0	41.0
27	38.35562867287016	40.0	37.0	41.0	34.0	41.0
28	38.30836829362179	40.0	37.0	41.0	34.0	41.0
29	38.25865308812368	40.0	36.0	41.0	34.0	41.0
30	38.30312196487128	40.0	37.0	41.0	34.0	41.0
31	38.26289296794172	40.0	36.0	41.0	34.0	41.0
32	38.14395448982687	40.0	36.0	41.0	34.0	41.0
33	37.98608914122584	40.0	36.0	41.0	34.0	41.0
34	38.02611497304727	40.0	36.0	41.0	34.0	41.0
35	38.04329717474887	40.0	35.0	41.0	34.0	41.0
36	38.095252205214805	40.0	36.0	41.0	34.0	41.0
37	38.10510127027982	40.0	35.0	41.0	34.0	41.0
38	37.98700237583856	40.0	35.0	41.0	34.0	41.0
39	37.927263042962146	39.0	35.0	41.0	34.0	41.0
40	37.898082710905676	39.0	35.0	41.0	34.0	41.0
41	37.850924369957184	39.0	35.0	41.0	34.0	41.0
42	37.776555881420045	39.0	35.0	41.0	34.0	41.0
43	37.64821321980654	39.0	35.0	41.0	34.0	41.0
44	37.54478564023504	39.0	35.0	41.0	34.0	41.0
45	37.393720462755375	39.0	35.0	41.0	33.0	41.0
46	37.129490994589446	38.0	35.0	40.0	33.0	41.0
47	36.921572202293376	38.0	35.0	40.0	32.0	41.0
48	36.79600029976593	38.0	35.0	40.0	32.0	41.0
49	36.95914684388226	38.0	35.0	40.0	32.0	41.0
50	36.63181883448978	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	9.0
21	21.0
22	88.0
23	240.0
24	611.0
25	1435.0
26	2869.0
27	6276.0
28	12135.0
29	23676.0
30	44078.0
31	79596.0
32	140740.0
33	269702.0
34	570078.0
35	326834.0
36	495471.0
37	806146.0
38	1019640.0
39	1527850.0
40	2127461.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	51.96228390732024	18.273844759214704	29.763871333465058
2	23.349364268974714	24.275968713987908	33.21445947202138	19.160207545015997
3	22.441941681519193	25.04298815245311	28.89634164417493	23.618728521852763
4	20.613655792431377	26.87756515426439	32.564060361952265	19.944718691351973
5	17.65231233268692	30.489307724077968	32.12032899970905	19.738050943526062
6	18.158485336140593	29.91312764748199	30.431265837608002	21.49712117876941
7	23.10791431370332	29.053780428898285	25.194417516067492	22.643887741330907
8	24.09450565871505	28.052500878274213	28.721424565467213	19.131568897543527
9	23.7000166734608	29.470885226629623	25.776124113861897	21.05297398604768
10	24.15213171470649	26.378233232876735	25.595693927842145	23.87394112457463
11	23.374971378119884	27.117445684826517	26.785747101829237	22.721835835224354
12	23.91246580632682	26.375148039003825	26.574585702058002	23.137800452611355
13	23.180537094694394	24.9249191243254	27.052093230266394	24.842450550713814
14	20.512796780559952	26.7832387050717	27.95970361213157	24.74426090223678
15	20.01940721084926	32.75774346773913	27.76441083997623	19.458438481435383
16	19.656494514189944	34.76853869523932	25.32353958659207	20.25142720397867
17	21.69437237833974	34.999995305139755	23.725382332650703	19.580249983869802
18	21.68399003026307	28.02060265640558	24.02424372173107	26.271163591600278
19	21.882636274019408	26.811501763724856	25.01346418847798	26.292397773777758
20	23.142589210057572	27.6361606720679	24.580732213086023	24.640517904788503
21	22.824264271805045	31.419238812919343	25.155007517812635	20.601489397462977
22	22.256427632552338	29.207208461586852	24.96330966721355	23.57305423864726
23	23.986738495345584	28.652208911622743	27.029249381720437	20.331803211311232
24	22.891119081642415	29.4298789758927	25.891443295276794	21.787558647188092
25	24.38699145464708	28.201084983326467	25.523864817527254	21.888058744499197
26	24.156692681399612	31.348913007070255	24.1121643542333	20.38222995729683
27	23.82233292669855	28.028388745427087	24.963591173261594	23.185687154612772
28	24.429752135274	26.78024111441498	25.78556047107129	23.004446279239726
29	24.24083061513996	27.38426877586425	27.159015441747837	21.215885167247954
30	25.176371780115787	27.646168661046524	28.23166634263432	18.945793216203366
31	24.53389074070787	29.344458967477305	26.149208937279116	19.97244135453571
32	21.923695926397478	27.827236625391038	26.879563566938586	23.3695038812729
33	23.567347300750736	24.87789369028917	31.293361615664356	20.261397393295734
34	22.90058936833475	28.90356879272693	27.922904741770587	20.272937097167727
35	22.11377910035137	26.747862310796645	26.958857975692435	24.17950061315955
36	22.707358617597	28.046077045360402	25.552036837687147	23.69452749935545
37	22.517593019717925	31.477626561779225	24.992776924435894	21.012003494066956
38	26.72544119414391	27.244412465294577	25.54994037292345	20.48020596763806
39	23.003940216047244	30.830103918329392	26.001870876158634	20.16408498946473
40	21.056765092158575	29.066815935595216	27.760082242631267	22.116336729614943
41	23.02357667946562	30.881795331734036	24.62456128772365	21.470066701076693
42	21.24283015654516	30.73286698426736	26.97062688376846	21.05367597541902
43	21.4505249744373	26.244651320977948	27.005041502260173	25.29978220232458
44	22.226231585883184	26.946358889168803	25.50682469971654	25.320584825231467
45	21.61905941514206	27.867867292638866	29.412728191523986	21.100345100695087
46	22.238933639081896	27.47262141301824	28.048290082438015	22.24015486546185
47	21.620454296470957	31.035550181575093	27.791243216721618	19.552752305232335
48	24.215649909325535	28.764857976433383	25.86001261948379	21.15947949475729
49	21.241155818114223	27.34959152021722	26.09027184004203	25.318980821626525
50	22.365903821545764	27.633726651153555	28.45972574739536	21.540643779905317
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	275702.0
1	276066.0
2	190385.5
3	103173.0
4	96744.0
5	66050.0
6	28067.0
7	16247.0
8	16877.5
9	15656.0
10	13724.0
11	12388.0
12	11409.5
13	11675.5
14	14695.0
15	18976.0
16	21876.5
17	22364.5
18	22736.0
19	27769.0
20	36728.0
21	46370.5
22	54324.5
23	71353.5
24	91105.5
25	101444.5
26	107922.5
27	118178.5
28	132417.5
29	146986.5
30	167201.5
31	213784.5
32	276527.5
33	301042.5
34	311440.0
35	331371.0
36	344766.5
37	367505.0
38	389019.5
39	395669.0
40	406572.0
41	435340.5
42	461957.0
43	473003.0
44	480731.5
45	475613.5
46	492158.5
47	511415.0
48	539456.0
49	571298.0
50	616229.0
51	658869.5
52	598227.0
53	523769.0
54	505750.5
55	481211.0
56	448568.0
57	435876.0
58	420555.0
59	362310.5
60	286223.5
61	237854.0
62	198676.5
63	160840.0
64	130512.5
65	108828.5
66	76828.0
67	47588.5
68	35620.5
69	28568.5
70	21180.0
71	13507.5
72	7645.0
73	4182.5
74	2562.0
75	2004.0
76	1578.5
77	1096.0
78	602.5
79	334.0
80	196.0
81	121.0
82	95.5
83	79.5
84	71.5
85	53.5
86	26.0
87	8.5
88	1.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	62405.0
25	78111.0
26	65516.0
27	78889.0
28	86904.0
29	72519.0
30	81394.0
31	124239.0
32	174696.0
33	31698.0
34	26891.0
35	24491.0
36	26440.0
37	30428.0
38	20644.0
39	20486.0
40	24726.0
41	24414.0
42	37284.0
43	55329.0
44	147226.0
45	428288.0
46	811940.0
47	690036.0
48	1333006.0
49	1002649.0
50	1894312.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.675236094377187
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.66872225558953	6.934118084051734
2	11.489867719407536	2.223343657568622
3	4.489821674959171	1.3032025418064606
4	2.3429685794821826	0.9067509667279063
5	1.3950949934683383	0.6748936717944887
6	0.9190380082916911	0.5335145825956973
7	0.7284385196118306	0.49334702602381486
8	0.5383847463992106	0.41671996248190013
9	0.44682268367457906	0.3890803461187291
>10	3.715527138761664	7.334030931583207
>50	0.5600419541217163	3.847294450053628
>100	1.354211595515701	35.532338349993324
>500	0.28302078942155273	17.42035596555942
>1k	0.05974883332232779	10.49053861692751
>5k	0.005145832534937321	3.133022035989802
>10k+	0.0031446754380172522	8.36744881072376
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	52658	0.7063484302600644	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	36941	0.495522377648924	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	35418	0.4750930286556831	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	34026	0.45642089878136183	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	28207	0.37836549379668116	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27738	0.3720743810732209	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	27344	0.36678930983005814	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	20900	0.2803502258428984	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18403	0.24685575149219424	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10729	0.14391758722815587	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG	10152	0.13617777477306722	No Hit
AAGATAAAATTTGAAATCTGGTTAGGCTGGTGTTAGGGTTCTTTGTTT	9722	0.13040980361936166	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	7935	0.10643918861547365	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7533	0.10104680628107914	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0911473581149519	0.0	0.0	0.0	0.0
2	0.14155674322105777	0.0	0.0	0.0	0.0
3	0.18316661884616164	0.0	0.0	0.0	0.0
4	0.2386464530129668	0.0	0.0	0.0	0.0
5	0.27878080113363435	0.0	0.0	0.0	0.0
6	0.36106158033556446	0.0	0.0	0.0	0.0
7	0.40401284460106496	0.0	0.0	0.0	0.0
8	0.45597824053003094	0.0	0.0	0.0	0.0
9	0.5081582586414604	0.0	0.0	0.0	0.0
10	0.5496205815161206	0.0	0.0	0.0	0.0
11	0.5941815121500971	0.0	0.0	0.0	0.0
12	0.6370657069835778	0.0	0.0	0.0	0.0
13	0.676958605148974	0.0	0.0	0.0	0.0
14	0.7152552508322981	0.0	0.0	0.0	0.0
15	0.7556981183402569	0.0	0.0	0.0	0.0
16	0.7919558532901781	0.0	0.0	0.0	0.0
17	0.8361143673320357	0.0	0.0	0.0	1.3413886403966432E-5
18	0.8687101112936741	0.0	0.0	0.0	1.3413886403966432E-5
19	0.9035996298303908	0.0	0.0	0.0	1.3413886403966432E-5
20	0.9389452205048423	0.0	0.0	0.0	1.3413886403966432E-5
21	0.9685228400255883	0.0	0.0	0.0	1.3413886403966432E-5
22	0.9968395542243614	0.0	0.0	0.0	1.3413886403966432E-5
23	1.0259879293801806	0.0	0.0	0.0	1.3413886403966432E-5
24	1.055364340604867	0.0	0.0	0.0	1.3413886403966432E-5
25	1.0831579132338855	0.0	0.0	0.0	1.3413886403966432E-5
26	1.1122928745033005	0.0	0.0	0.0	1.3413886403966432E-5
27	1.140435208178822	0.0	0.0	0.0	1.3413886403966432E-5
28	1.16765198369247	0.0	0.0	0.0	1.3413886403966432E-5
29	1.1962772172785343	0.0	0.0	0.0	1.3413886403966432E-5
30	1.2254390063207574	0.0	0.0	0.0	1.3413886403966432E-5
31	1.2518643625365713	0.0	0.0	0.0	1.3413886403966432E-5
32	1.278759204776524	0.0	0.0	0.0	1.3413886403966432E-5
33	1.3074649216810121	0.0	0.0	0.0	1.3413886403966432E-5
34	1.3361438108126924	0.0	0.0	0.0	1.3413886403966432E-5
35	1.3620460254587516	0.0	0.0	0.0	1.3413886403966432E-5
36	1.3849971850959382	0.0	0.0	0.0	1.3413886403966432E-5
37	1.3999536684363607	0.0	0.0	0.0	1.3413886403966432E-5
38	1.413407796499539	0.0	0.0	0.0	1.3413886403966432E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTCG	40	1.8189894E-11	136.69775	44
AGGTACG	75	0.0	135.39587	44
TCGAATG	65	0.0	132.19124	44
TGACGGC	155	0.0	131.02827	44
CCGATAA	90	0.0	130.18832	44
TATGCGC	55	0.0	127.821266	44
TAGTACG	25	4.4630324E-6	124.980804	44
CTACCGC	120	0.0	123.67892	44
CATCTAG	65	0.0	120.17385	44
CTCGCCC	130	0.0	120.17385	44
ACACCGA	105	0.0	101.98733	43
GATCGTC	35	1.8735591E-10	101.98732	43
CAACGTT	30	7.528797E-9	101.98732	43
CCCGTGT	70	0.0	100.431	44
TCTATAC	150	0.0	98.587746	43
ACGACTG	40	3.9253973E-7	97.64125	44
AGCGCCT	550	0.0	95.15583	44
GGTGCAT	95	0.0	85.88406	43
CAGGCGA	320	0.0	85.43609	44
GGCGTGC	125	0.0	81.58986	43
>>END_MODULE