FastQCFastQC Report
Tue 24 May 2016
ERR841134.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841134.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132090
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC104830.9259864498405604No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA84680.7479970673709688No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA61480.5430663639816622No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT49330.4357427413014866No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG46130.4074764373857202No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA35540.3139326378644807No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT32280.2851363407502937No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT31220.2757731275781961No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC30050.26543826020899397No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGA28750.2539550742432139No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA28630.25289508784637266No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC27430.24229522387796024No Hit
TCTCCCAGACAGTGGCTCAGCCTTCAGGACTCTCTTCCAGCATGGCCG25560.22577710252718425No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTAC22940.2026340661961505No Hit
CTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAAG21770.19229919882694838No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA20150.17798938246959164No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGA19830.17516275207801502No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATT18210.16085293572065826No Hit
TTAGTTGTGGCAATAAAAATGATTAAGGATACTAGTATAAGAGATCAG17750.15678965453276683No Hit
ACTTCTCTCTTCTGCAGTTAGGTGAAGAAGGATGTGTTTGCTTCCTTTT17320.15299136994408571No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCA16540.14610145836461766No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAAG16530.14601312616488088No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG16440.14521813636724995No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC16060.1418615127772527No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA15500.13691490959199357No Hit
TCAAGAGATCAAGACCATCCTGGCCAACATGGTAAAACCCTGTCTTTA14960.132144970806208No Hit
CTCTTCCCTTTTGCGGCCATCACCGAAGCGGGAGCGGCCAAAATGAAGT14410.12728669982068563No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA14350.12675670662226501No Hit
CTCTTCCCTTTTGCGGCCATCACCGAAGCGGGAGCGGCCAAAATGAAGTT14210.12552005582595024No Hit
TCTCCCAGACAGTGGCTCAGCCTTCAGGACTCTCTTCCAGCATGGC14010.12375341183121483No Hit
AGTTCAGATTCAGACTCTTCCTCAGAAGATGAAGAGTTCCATGATGGCTA13920.12295842203358391No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG13830.12216343223595298No Hit
CTCTCGAGTCACTCCGGCGCAGTGTTGGGACTGTCTGGGTATCGGAAAG13540.11960179844358663No Hit
ATGGCCACCCTGGAGAACCTGCTGAGCCACACAATCATACTCAAAAACTT13440.11871847644621895No Hit
CTCATCAAGAGAACCTTACTGATTGAAAGACTCCACTGGAGGTCAGTCA13280.11730516125043063No Hit
AGTTGAGAAATTTTCAAAGATTTTTGCGAGGTGTTGTAGAGATCAGCTAT13030.11509685625701135No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12940.11430186645938045No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT12810.11315354786280243No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12740.11253522246464503No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA12730.11244689026490827No Hit
TCTCCCAGAGACAGAGATCTGCAATAGGAACTTACCAGACAAAGGAGT12680.11200522926622442No Hit
ATCCAGCGGAGCCAGCTGGAGAAAAACCTTCTTGACCTGCTTCACACCA12590.11121023946859347No Hit
AGAAGCAGGAGGCTAGCCTGAGCCCAAGTTTCAGATCAGCCTGCTCAA12500.11041524967096257No Hit
ACTTCCCTCCATGTGATGCTGTCCACCATGTTATGATGCAGGATGAGG12430.10979692427280517No Hit
TAGGAAGTGCTACCGTAGTAGAATAATCTAGAGGGGAGTGATTAACAGTG12430.10979692427280517No Hit
ATAAAAAGTATTTGTACGGGCCAGGCGCCACACCTATAATCCCAGCACT12420.1097085920730684No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACT12330.10891360227543746No Hit
ATCTGGACAGTGCAGGCAGCTACAAAGCATCAGTTCTGTGTGGTGGGG12240.10811861247780653No Hit
ATTTGAGAAGGAGGCTGCTGAGATGGGAAAGGGCTCCTTCAAGTATGCCT12130.10714695828070206No Hit
CTCTCTTCCCTGCCACCATGTAGGATGTAACTTGTTACCCCTTGCCTTC12120.10705862608096528No Hit
CTTCCATGGGGAGGATGCTGAGTGAGCAGTTTCCTACCCCGTGGATCTAG12010.10608697188386083No Hit
CGGGCGAGGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACA11990.10591030748438729No Hit
ATGCCATGATGAGAAAAACGATTAAAAAACAGTATGACCAAATTGAGCGG11960.10564531088517698No Hit
TTAGAATAAGGGTGCAATTCACAAATGGGTGGACAGGGTAGGCCTTACTG11870.10485032108754605No Hit
AGCCCAAATATCCTCGGAAGAGCGCTCCCAGGAGAAACAAGCTTGACCAC11860.10476198888780927No Hit
AGCTGGAAATTAAAAGGAAAGAGAGAGAAGCCAAAGAGATGCATGAAAG11820.1044086600888622No Hit
CCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGCGCC11790.10414366348965187No Hit
CCTGTTGACTGCGTCGCGATGTGTGGCGACTGTGTGGAGAAGGAATATC11760.10387866689044156No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT11740.10370200249096803No Hit
ACTCAAAGTCTTCAAGCCTGGAGTTCCTGCTTGGCTCTTCCTGAGGTCT11570.10220035509544295No Hit
TGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACAGAGAAGA11470.10131703309807524No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAAC11470.10131703309807524No Hit
CTCCTGAGGGAAGAGGAGTGGAGTAGGGGGAACGCGGCGGCGGCGTTGAC11440.10105203649886493No Hit
CTTTTTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAA11430.10096370429912817No Hit
AGCAAGAGGATTTAACAACTGTAAATATATATTAATATAGTTTGGATATT11360.10034537890097077No Hit
TCTCTCGAGTCACTCCGGCGCAGTGTTGGGACTGTCTGGGTATCGGAAA11350.100257046701234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAC301.4551915E-11250.46244
CTACTGG750.0250.46244
ATATCTC301.4551915E-11250.46244
CTAGTCC500.0250.46244
ATTGGAC500.0250.46244
CGGTAAA251.4097168E-9250.46244
TACAATG1000.0250.46244
CTGTGCA600.0250.46244
CTGAACC100.0013809397250.4619944
ATATCCA100.0013809397250.4619944
ACCAGTA400.0250.4619944
CCCCCTA900.0250.4619944
GTACTCT800.0250.4619944
GACGCGT650.0250.4619944
GATCTAG900.0250.4619944
GACTCGC800.0250.4619944
TACGTTG100.0013809397250.4619944
AATCGTA201.3959107E-7250.4619944
ACAATTG950.0237.279844
TATGCCT1050.0226.6084744
Produced by FastQC (version 0.11.5)