##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841133.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6206994
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.46288122720918	39.0	37.0	39.0	35.0	39.0
2	37.94454239846212	39.0	38.0	39.0	35.0	39.0
3	38.00464991588521	39.0	38.0	39.0	35.0	39.0
4	38.360844879179844	39.0	38.0	40.0	35.0	41.0
5	39.25239705403292	40.0	39.0	41.0	36.0	41.0
6	39.169650880925616	40.0	38.0	41.0	36.0	41.0
7	39.213541691839886	40.0	38.0	41.0	36.0	41.0
8	39.16463911516589	40.0	38.0	41.0	36.0	41.0
9	39.06903583280409	40.0	38.0	41.0	35.0	41.0
10	39.085719109765535	40.0	38.0	41.0	35.0	41.0
11	38.967627324917665	40.0	38.0	41.0	35.0	41.0
12	38.846665229578115	40.0	38.0	41.0	35.0	41.0
13	38.73996978247442	40.0	38.0	41.0	35.0	41.0
14	38.69942906340815	40.0	38.0	41.0	35.0	41.0
15	38.638461709484496	40.0	38.0	41.0	35.0	41.0
16	38.66097921151527	40.0	38.0	41.0	35.0	41.0
17	38.781558835081846	40.0	38.0	41.0	35.0	41.0
18	38.80540242184864	40.0	38.0	41.0	35.0	41.0
19	38.78552242840899	40.0	38.0	41.0	35.0	41.0
20	38.806177031909485	40.0	38.0	41.0	35.0	41.0
21	38.86740618727841	40.0	38.0	41.0	35.0	41.0
22	38.85519222348209	40.0	38.0	41.0	35.0	41.0
23	38.82981407747454	40.0	38.0	41.0	35.0	41.0
24	38.71971553379945	40.0	38.0	41.0	35.0	41.0
25	38.67726209882238	40.0	38.0	41.0	35.0	41.0
26	38.65173118877864	40.0	38.0	41.0	35.0	41.0
27	38.57097417791248	40.0	37.0	41.0	35.0	41.0
28	38.50465798095218	40.0	37.0	41.0	34.0	41.0
29	38.477574639632266	40.0	37.0	41.0	34.0	41.0
30	38.51580845397012	40.0	37.0	41.0	35.0	41.0
31	38.47715098681546	40.0	37.0	41.0	34.0	41.0
32	38.31073760081165	40.0	37.0	41.0	34.0	41.0
33	38.162265444860545	40.0	37.0	41.0	34.0	41.0
34	38.188444400715305	40.0	36.0	41.0	34.0	41.0
35	38.196790197784175	40.0	36.0	41.0	34.0	41.0
36	38.17238501508564	40.0	36.0	41.0	34.0	41.0
37	38.14561944033949	40.0	36.0	41.0	34.0	41.0
38	38.06413788678775	40.0	36.0	41.0	34.0	41.0
39	38.01951067695208	39.0	36.0	41.0	34.0	41.0
40	37.91043658918014	39.0	35.0	41.0	34.0	41.0
41	37.792430688012736	39.0	35.0	41.0	34.0	41.0
42	37.70604119761817	39.0	35.0	41.0	34.0	41.0
43	37.61918757620009	39.0	35.0	41.0	34.0	41.0
44	37.43073369297925	39.0	35.0	41.0	34.0	41.0
45	37.24391191422292	38.0	35.0	40.0	33.0	41.0
46	36.9177569422111	37.0	35.0	40.0	33.0	41.0
47	36.69149603810425	37.0	35.0	40.0	32.0	41.0
48	36.574599831365745	37.0	35.0	40.0	32.0	41.0
49	36.73237032776327	37.0	35.0	40.0	33.0	41.0
50	36.17348498006514	37.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	10.0
21	30.0
22	79.0
23	186.0
24	395.0
25	824.0
26	1730.0
27	3666.0
28	7636.0
29	15328.0
30	30337.0
31	54964.0
32	92150.0
33	155357.0
34	267188.0
35	307956.0
36	509215.0
37	751479.0
38	935090.0
39	1394563.0
40	1678809.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	52.00644627657124	18.300871565205316	29.692682158223448
2	23.058601313292716	24.354993737709428	32.009407452303	20.576997496694858
3	23.7452460885253	24.75099863154371	26.166015304670825	25.337739975260163
4	19.20319239876823	26.13255305225041	31.08491807789729	23.57933647108407
5	16.9771551253312	27.28876489972441	30.334023200280203	25.400056774664193
6	20.74624206177741	27.917700580989766	28.69274563500464	22.643311722228184
7	21.35120478608486	26.68990819066363	24.865015174817312	27.093871848434205
8	18.865347702929952	28.19922171666349	26.16865748541081	26.766773094995745
9	21.877208194498014	27.798560784817898	27.17511890618873	23.149112114495356
10	24.78189925751499	24.59374054494011	26.93413268967233	23.69022750787257
11	22.569443437515808	26.357541186603374	27.260651452216646	23.812363923664176
12	21.49802303659388	28.3352940247727	27.312657302391464	22.854025636241957
13	20.794397416849446	28.17636040891936	25.739254782588798	25.289987391642395
14	17.629209888071422	30.50471774259811	24.763275105469734	27.102797263860733
15	19.263253678028367	31.55293850775432	27.14204331436441	22.04176449985291
16	21.208365917543983	32.54510959733488	23.743409450693846	22.503115034427292
17	20.516243450533384	35.03969876561827	20.878786091947248	23.565271691901106
18	18.83368986662465	31.687029180308535	23.251883278765856	26.227397674300956
19	21.372068347415834	29.09952869295508	24.85035429388203	24.67804866574706
20	22.19612263198579	27.617893621292367	27.082030367678783	23.10395337904306
21	19.455826765742003	32.0981299482487	24.28829478488299	24.15774850112631
22	21.611379034682486	30.114657755428798	24.114120297200223	24.159842912688493
23	24.095173927991553	29.41214378489813	26.24060857800088	20.252073709109432
24	19.24433953053604	31.570982668905433	26.617586548335638	22.567091252222895
25	21.2336146932598	30.610088658180086	25.11555237078349	23.040744277776625
26	21.712133168919213	32.854677669765564	25.163965926770526	20.269223234544697
27	23.098732573189118	31.402737733831344	24.124754914633556	21.373774778345986
28	22.51931967716321	29.17486485000884	24.27035892314447	24.035456549683474
29	20.799679483697307	31.94392235001163	25.354233527950825	21.902164638340242
30	22.88635159125972	30.716186942157535	25.97443435311128	20.423027113471466
31	22.91297565172292	30.086556072912106	24.22644380933842	22.774024466026553
32	20.23817880475244	30.553306940101383	27.046826950973113	22.161687304173068
33	18.88708337732168	26.96426307276141	30.214042423686188	23.934611126230724
34	20.301757124125388	29.219317016719828	27.351583422148284	23.127342437006504
35	18.983915521689994	27.498189656294013	27.50323348862277	26.014661333393217
36	19.43552087703307	28.27832346855989	29.41458078216308	22.87157487224395
37	17.708255466939608	29.399211656671014	27.04958124651398	25.842951629875394
38	19.579378067260834	29.2053744328589	25.624196333609543	25.591051166270727
39	20.085517907306873	29.770486201731362	28.002471661946	22.141524229015765
40	17.617641014214875	28.02625152658492	30.247233488626772	24.108873970573434
41	18.05694068719552	30.261104753978984	26.347339764163095	25.334614794662397
42	18.163064594731075	28.844139549140113	27.71853341536399	25.27426244076483
43	18.476578721698495	26.320681421580787	28.314447482073447	26.888292374647264
44	18.426904048153705	27.939235605281915	25.884147177965378	27.749713168599005
45	18.497787368442005	26.375861839320315	28.893232585429928	26.233118206807752
46	18.43055229297425	29.95280828090612	27.412873253635983	24.203766172483647
47	20.161564427441657	29.244445877023313	27.054308076032413	23.539681619502613
48	20.084418442598498	30.49375614226067	24.50670540676541	24.91512000837543
49	18.760036717423024	30.545484958196827	24.829767843064584	25.864710481315562
50	17.850040689357733	30.502316788929896	26.969465743203642	24.678176778508735
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	174552.0
1	172839.5
2	120663.5
3	69168.5
4	64865.5
5	45094.5
6	19588.5
7	11362.0
8	12299.0
9	12366.0
10	11976.5
11	11180.0
12	10103.0
13	10320.5
14	13039.0
15	15312.0
16	15569.0
17	15178.0
18	15574.0
19	17867.0
20	20389.0
21	22437.5
22	24629.0
23	32091.0
24	41979.0
25	50754.0
26	63273.0
27	79507.5
28	93847.5
29	105707.0
30	122465.0
31	158847.5
32	208612.0
33	223335.5
34	244486.0
35	306254.0
36	326939.5
37	323389.5
38	337033.0
39	350715.0
40	372547.5
41	403568.0
42	422662.5
43	445044.0
44	480255.5
45	493903.0
46	507831.5
47	527761.0
48	553015.5
49	610292.0
50	681575.0
51	679188.5
52	615979.5
53	538978.0
54	441263.0
55	363296.5
56	308446.5
57	260193.5
58	222494.0
59	185704.0
60	137474.0
61	92499.5
62	64642.5
63	47553.0
64	35697.0
65	30246.0
66	20197.0
67	10330.5
68	6666.5
69	5024.0
70	3379.5
71	2417.0
72	1744.5
73	1183.5
74	709.0
75	439.5
76	284.0
77	181.5
78	91.0
79	56.0
80	44.5
81	26.5
82	11.0
83	10.5
84	9.5
85	10.5
86	9.0
87	5.0
88	3.0
89	3.0
90	1.5
91	0.0
92	0.0
93	0.0
94	0.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	33170.0
25	38098.0
26	43461.0
27	52309.0
28	57109.0
29	48712.0
30	57251.0
31	93078.0
32	128899.0
33	24286.0
34	19808.0
35	21058.0
36	24667.0
37	31853.0
38	19536.0
39	20228.0
40	23674.0
41	27929.0
42	33410.0
43	50781.0
44	132225.0
45	380958.0
46	687267.0
47	603975.0
48	1114083.0
49	901912.0
50	1537257.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.932944941755444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.54611676893829	7.822097554312083
2	11.583379326987682	2.5328089684284913
3	4.70708815021657	1.5438700674692167
4	2.4596194968160305	1.0756353814543156
5	1.5068682228710384	0.8237253657564966
6	0.9976361227921544	0.6544260481435799
7	0.7343066763644072	0.5619694124138849
8	0.5515343478320461	0.48239157266678057
9	0.42783168643924574	0.42097142449607877
>10	3.408842670303279	7.470244609029594
>50	0.5241627269235294	4.118917282178209
>100	1.2702109049919277	37.04257096836309
>500	0.22971579059028585	15.93131138067595
>1k	0.04485930904056696	9.220156395903874
>5k	0.0030106918819172456	2.134947071530595
>10k+	0.004817107011067593	8.163956497177772
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	24870	0.4006770427037629	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	20903	0.3367652683408426	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	19950	0.3214116205042247	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	19792	0.31886610491326395	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	19748	0.3181572271537559	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	16983	0.27361070431194234	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15442	0.2487838718709894	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	13250	0.21346887076095128	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	13163	0.21206722610010575	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	13083	0.2107783574464548	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	12983	0.20916727162939097	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGA	12916	0.20808784413195824	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	12123	0.19531193360264246	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	11540	0.18591930328916056	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	10858	0.1749316980167856	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10422	0.16790736385438748	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCA	8085	0.13025628830960687	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	7958	0.12821020932193586	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACAC	7291	0.11746426692212043	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	6628	0.10678276795498755	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	6374	0.10269060997964553	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0426293307195077	0.0	0.0	0.0	0.0
2	0.08282592185524909	0.0	0.0	0.0	0.0
3	0.12717911439901505	0.0	0.0	0.0	0.0
4	0.18144048471772325	0.0	0.0	0.0	0.0
5	0.226115894424902	0.0	0.0	0.0	0.0
6	0.29921085794508584	0.0	0.0	0.0	0.0
7	0.34585179234908237	0.0	0.0	0.0	0.0
8	0.4030614497130173	0.0	0.0	0.0	0.0
9	0.4590627927141544	0.0	0.0	0.0	0.0
10	0.5102953216967827	0.0	0.0	0.0	0.0
11	0.566731657868527	0.0	0.0	0.0	0.0
12	0.6137914745849601	0.0	0.0	0.0	0.0
13	0.66077073701054	0.0	0.0	0.0	0.0
14	0.7070733433929531	0.0	0.0	0.0	0.0
15	0.7579353226376568	0.0	0.0	0.0	0.0
16	0.8076534309522452	0.0	0.0	0.0	0.0
17	0.8687296942771332	0.0	0.0	0.0	0.0
18	0.9092323917181167	0.0	0.0	0.0	0.0
19	0.9511367338199457	0.0	0.0	0.0	0.0
20	0.9937660645394534	0.0	0.0	0.0	0.0
21	1.0304343777358251	0.0	0.0	0.0	0.0
22	1.063268306687585	0.0	0.0	0.0	0.0
23	1.1011449342467545	0.0	0.0	0.0	0.0
24	1.1386349012098287	0.0	0.0	0.0	0.0
25	1.173676017730966	0.0	0.0	0.0	0.0
26	1.2183192057218035	0.0	0.0	0.0	0.0
27	1.2527158879161153	0.0	0.0	0.0	0.0
28	1.2890458730909036	0.0	0.0	0.0	0.0
29	1.326310288039589	0.0	0.0	0.0	0.0
30	1.3614158479934086	0.0	0.0	0.0	0.0
31	1.394137000937974	0.0	0.0	0.0	0.0
32	1.4280986899616788	0.0	0.0	0.0	0.0
33	1.4597887479833234	0.0	0.0	0.0	0.0
34	1.4957965159946989	0.0	0.0	0.0	0.0
35	1.5317076188570506	0.0	0.0	0.0	0.0
36	1.5601271726700559	0.0	0.0	0.0	0.0
37	1.5790735418787258	0.0	0.0	0.0	0.0
38	1.597439920193253	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGTAG	80	0.0	161.12445	44
TAGTTCG	75	0.0	128.89955	44
TCGTAAG	140	0.0	126.59777	44
CCTCGAT	110	0.0	124.505264	44
ACGAAGC	145	0.0	122.23234	44
GCATACG	140	0.0	120.84333	44
AACTGTC	90	0.0	116.36766	44
TAAACGC	155	0.0	103.951256	44
TACGATT	95	0.0	101.32602	43
ATAGCGG	170	0.0	98.34585	43
TTCGTAA	120	0.0	92.88219	43
TCGCAAT	55	0.0	92.11456	43
CGATAGG	55	0.0	92.11456	43
ATGCGTA	90	0.0	90.06758	43
CAGTTCG	135	0.0	90.06757	43
GTCCAAC	135	0.0	90.06757	43
TGGCCGA	460	0.0	89.21095	43
AATCGCA	325	0.0	86.759315	44
TGTCACG	95	0.0	85.32718	43
TTATACG	85	0.0	85.30118	44
>>END_MODULE