FastQCFastQC Report
Tue 24 May 2016
ERR841132.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841132.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2312295
Sequences flagged as poor quality0
Sequence length24-50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT157230.6799737922713148No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC137530.594777050506099No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA99110.4286217805254088No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC84740.3664757308215431No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA79780.3450251806106055No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA53580.2317178387705721No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG53550.23158809753945755No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA49710.21498121995679617No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA48590.2101375473285199No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA41210.1782212044743426No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT40270.1741559792327536No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC32990.14267210714895806No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT32050.13860688190736908No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG31360.1356228335917346No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC28920.12507054679441854No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT27980.12100532155282955No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA26650.11525346030675152No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG26330.11386955384152973No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG26030.1125721415303843No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA24950.1079014572102608No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA24520.10604183289761902No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA24040.10396597319978637No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA23220.10041971288265554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCTA1000.0150.2249344
CTGGTCG100.0063781403150.2249344
CCCGTTG100.0063781403150.2249344
CCGACAA100.0063781403150.2249344
TAGGCGT151.0680277E-4150.2249344
TCGACTA700.0150.2249344
CGTGCCC550.0150.2249144
ACGATCA950.0150.2249144
ACGGCCA1900.0146.2716444
CCCATTA1200.0143.9655644
TTCACGG2000.0142.7136844
ACGCGGA850.0141.3881744
TATTGAC800.0140.8358644
TCTATAT800.0140.8358644
TTAGCTA750.0140.2099544
TTAGATT1700.0136.9697944
ACGTCTT550.0136.568144
TTGGACC500.0135.2024444
CCGACTT351.4861143E-9128.7642244
GACGCCC700.0128.7642244
Produced by FastQC (version 0.11.5)