##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841132.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2312295
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.891811814668976	39.0	37.0	39.0	33.0	39.0
2	37.43315364172824	39.0	37.0	39.0	34.0	39.0
3	37.604584622636814	39.0	37.0	39.0	35.0	39.0
4	37.97931276069879	39.0	37.0	39.0	35.0	41.0
5	38.87028212230706	40.0	38.0	41.0	35.0	41.0
6	38.92594197539674	40.0	38.0	41.0	35.0	41.0
7	38.995550740714314	40.0	38.0	41.0	35.0	41.0
8	38.87902192410571	40.0	38.0	41.0	35.0	41.0
9	38.77338834361532	40.0	38.0	41.0	35.0	41.0
10	38.796628025403336	40.0	38.0	41.0	35.0	41.0
11	38.70882391736348	40.0	38.0	41.0	35.0	41.0
12	38.67357192745735	40.0	38.0	41.0	35.0	41.0
13	38.54901948064585	40.0	37.0	41.0	35.0	41.0
14	38.51553197148288	40.0	37.0	41.0	35.0	41.0
15	38.56025204396498	40.0	37.0	41.0	35.0	41.0
16	38.616908742180385	40.0	37.0	41.0	35.0	41.0
17	38.68937959905635	40.0	37.0	41.0	35.0	41.0
18	38.70579013490926	40.0	38.0	41.0	35.0	41.0
19	38.608888139272885	40.0	37.0	41.0	35.0	41.0
20	38.6148571008457	40.0	37.0	41.0	35.0	41.0
21	38.664770282338544	40.0	37.0	41.0	35.0	41.0
22	38.709490354820645	40.0	37.0	41.0	35.0	41.0
23	38.65651182050733	40.0	37.0	41.0	35.0	41.0
24	38.55805465998067	40.0	37.0	41.0	35.0	41.0
25	38.5096239863165	40.0	37.0	41.0	35.0	41.0
26	38.53523119575583	40.0	37.0	41.0	35.0	41.0
27	38.513056568203844	40.0	36.0	41.0	35.0	41.0
28	38.48642106406982	40.0	36.0	41.0	35.0	41.0
29	38.4282348487003	40.0	36.0	41.0	35.0	41.0
30	38.46614273540862	40.0	36.0	41.0	35.0	41.0
31	38.420851253138075	40.0	36.0	41.0	35.0	41.0
32	38.290356836518065	40.0	36.0	41.0	34.0	41.0
33	38.15548195068836	40.0	36.0	41.0	34.0	41.0
34	38.174575711445016	40.0	36.0	41.0	34.0	41.0
35	38.20708286855052	40.0	36.0	41.0	34.0	41.0
36	38.22942061361376	40.0	35.0	41.0	34.0	41.0
37	38.17567992779845	40.0	35.0	41.0	34.0	41.0
38	38.110985046617586	40.0	35.0	41.0	34.0	41.0
39	38.03665495341565	40.0	35.0	41.0	34.0	41.0
40	38.01217231001504	40.0	35.0	41.0	34.0	41.0
41	37.98344313548602	39.0	35.0	41.0	34.0	41.0
42	37.91236234419178	39.0	35.0	41.0	34.0	41.0
43	37.848683083367504	39.0	35.0	41.0	34.0	41.0
44	37.750441453570545	39.0	35.0	41.0	34.0	41.0
45	37.56825683929446	39.0	35.0	41.0	34.0	41.0
46	37.33564804925149	39.0	35.0	41.0	33.0	41.0
47	37.14481822059414	38.0	35.0	40.0	33.0	41.0
48	37.046942036971735	38.0	35.0	40.0	33.0	41.0
49	37.33796791097814	38.0	35.0	40.0	33.0	41.0
50	36.907323188670375	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	3.0
21	14.0
22	42.0
23	97.0
24	241.0
25	520.0
26	1034.0
27	2047.0
28	3952.0
29	7301.0
30	13499.0
31	23654.0
32	40475.0
33	76039.0
34	219579.0
35	102715.0
36	124119.0
37	192154.0
38	268875.0
39	454147.0
40	781787.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	55.55467619832245	13.993889188014505	30.451434613663046
2	25.13407675058762	23.21165768208641	32.602544225542154	19.05172134178381
3	22.24166899119706	20.58569516432808	28.462242058214894	28.71039378625997
4	19.39358948577063	23.52883174508443	33.347648115832975	23.72993065331197
5	16.7518850319704	26.60996974866961	30.30400532803989	26.334139891320095
6	19.086837968338816	32.31309153892561	28.81695458408205	19.783115908653524
7	25.60698353800013	28.439364354461695	24.287861194181538	21.665790913356645
8	23.669471239612594	28.463063752678615	26.16240574840148	21.705059259307312
9	21.344162401423695	28.2723008958632	27.903057352111215	22.480479350601893
10	23.596729656034373	25.93207181609613	29.447280731913533	21.023917795955967
11	24.401255030175648	27.75856886772665	24.30874953239098	23.53142656970672
12	25.01073608687473	25.809769082232155	27.194929712688044	21.984565118205072
13	23.03149035914535	27.3075883483725	27.453590480453403	22.20733081202874
14	19.477834791841005	28.468296649000237	27.21975353490796	24.8341150242508
15	19.044282844533246	30.632812854761177	29.92174441409941	20.401159886606163
16	19.10526122315708	37.08151425315542	23.447656981483764	20.365567542203742
17	20.821434981263202	35.50745039019675	22.87225462149077	20.798860007049274
18	21.031486034437645	30.86595784707401	24.948719778401976	23.153836340086364
19	22.816033421341135	26.146361082820313	26.609710266207383	24.427895229631165
20	22.002858631792225	26.125602485841988	26.397237376718802	25.474301505646984
21	20.0666004986388	30.32165013547147	25.33638657697223	24.275362788917505
22	22.673923526193672	29.367403380624012	25.593879673657554	22.36479341952476
23	25.030629742312293	28.97004923679721	26.357406818766638	19.641914202123864
24	21.962336120607446	29.325194233434747	25.96956703188823	22.74290261406957
25	19.463636557361863	29.503033602953106	27.790812516770597	23.242517322914434
26	22.818767250921983	30.261353247630485	26.583711844278795	20.336167657168737
27	21.22951915180228	30.78238290942792	25.636385698533875	22.35171224023593
28	20.988182204486648	30.18167088804412	26.01708398965667	22.813062917812562
29	22.927183465104374	32.382970251976566	24.708936493942325	19.980909788976735
30	23.594226162072594	30.62466884172833	25.855632625567853	19.925472370631223
31	23.13401247537784	29.322670740259433	27.087997430613754	20.45531935374898
32	24.322118176174985	29.622526450312066	26.594607208194287	19.460748165318666
33	21.89236126473488	29.368093625142688	28.04800295826986	20.691542151852573
34	22.909690169977004	31.05334920887754	23.943126438993676	22.093834182151785
35	17.944315975510044	29.52998165220103	27.62964045244792	24.896061919841003
36	20.353692284144508	30.784731866767707	29.93167670951427	18.929899139573514
37	20.11181631562932	30.87059209230946	26.606376233780793	22.41121535828043
38	23.721054956447055	27.505701278543043	26.157825389063195	22.615418375946707
39	20.463122223001843	30.402002188412037	27.653971095514514	21.48090449307161
40	17.831155428219926	30.77432453567101	29.42071004032559	21.973809995783473
41	20.236623360894733	30.382780937195587	26.060754595168834	23.319841106740853
42	19.590114180838224	30.573975034331742	27.484859834184032	22.351050950646002
43	19.329616090214856	28.511986232147702	27.063493438517263	25.09490423912018
44	20.346822044081755	27.42848015194645	26.63102346828841	25.59367433568338
45	20.136713598919933	25.7778571033289	30.549172673832224	23.53625662391895
46	20.7928173491011	32.104947792056876	26.110700492173084	20.991534366668947
47	21.81867221999197	32.48281312725813	25.817003713368123	19.881510939381776
48	19.993178860840796	31.96225057041957	25.021632859063825	23.02293770967581
49	20.558155285672534	30.907226123315283	25.37021439055771	23.16440420045447
50	20.448512792841115	33.95817951820659	26.903659090211463	18.68964859874083
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	29175.0
1	26853.0
2	21351.5
3	17761.0
4	16432.0
5	11104.5
6	4486.5
7	2249.5
8	2298.0
9	2654.0
10	3163.5
11	4000.5
12	4576.0
13	3878.0
14	3489.0
15	4513.0
16	6375.5
17	8649.0
18	10599.5
19	12556.5
20	14070.0
21	15842.0
22	18681.0
23	22413.5
24	28401.0
25	38854.0
26	47089.0
27	51265.5
28	55611.0
29	55601.5
30	57666.5
31	72420.5
32	94350.5
33	104020.5
34	109582.5
35	126844.0
36	140187.0
37	145770.0
38	148186.5
39	146275.0
40	149748.5
41	150914.0
42	150660.0
43	158087.0
44	166618.5
45	172203.0
46	176311.0
47	181136.5
48	186634.5
49	196007.0
50	212701.5
51	205813.0
52	172185.5
53	142501.0
54	121299.0
55	113203.0
56	107758.0
57	97708.0
58	92968.5
59	95254.5
60	87524.5
61	62429.0
62	41801.5
63	31604.0
64	22341.5
65	16840.0
66	11234.0
67	6345.0
68	4391.5
69	2775.5
70	1904.5
71	1609.0
72	1252.5
73	847.0
74	411.5
75	226.0
76	160.0
77	78.5
78	26.0
79	18.0
80	15.0
81	11.5
82	9.5
83	9.0
84	6.5
85	4.0
86	2.0
87	1.0
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	12884.0
25	16562.0
26	8285.0
27	10690.0
28	13506.0
29	10125.0
30	17144.0
31	28985.0
32	46913.0
33	6382.0
34	5413.0
35	5532.0
36	6928.0
37	8508.0
38	5146.0
39	5665.0
40	6370.0
41	6626.0
42	12747.0
43	18211.0
44	53874.0
45	133287.0
46	278272.0
47	213239.0
48	453768.0
49	308254.0
50	618979.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.174858070167109
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.75528805268863	5.004842914216643
2	11.884399135862317	1.7053775409805751
3	4.779870982465744	1.028846876787058
4	2.6190559556979087	0.7516541903983348
5	1.5539901709204298	0.5574829459394405
6	1.0237659943759003	0.440722542402635
7	0.7616909016620754	0.38255168789341026
8	0.5168161798158925	0.29664661908359935
9	0.44540514796381214	0.28761468483259195
>10	3.5765875630847406	5.28112142827108
>50	0.6449174776244041	3.291580029725316
>100	1.661482315235753	32.077785030886986
>500	0.6269137992859362	31.250103671466363
>1k	0.14531540373191956	14.573392269970265
>5k	0.0032149425604406983	1.7104461335421075
>10k+	0.0012859770241762792	1.3598314336036021
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	15723	0.6799737922713148	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	13753	0.594777050506099	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	9911	0.4286217805254088	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	8474	0.3664757308215431	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	7978	0.3450251806106055	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	5358	0.2317178387705721	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	5355	0.23158809753945755	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	4971	0.21498121995679617	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	4859	0.2101375473285199	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	4121	0.1782212044743426	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	4027	0.1741559792327536	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	3299	0.14267210714895806	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	3205	0.13860688190736908	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	3136	0.1356228335917346	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	2892	0.12507054679441854	No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT	2798	0.12100532155282955	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	2665	0.11525346030675152	No Hit
CGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAG	2633	0.11386955384152973	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG	2603	0.1125721415303843	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA	2495	0.1079014572102608	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	2452	0.10604183289761902	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	2404	0.10396597319978637	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	2322	0.10041971288265554	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.040306275799584394	0.0	0.0	0.0	0.0
2	0.05898901308007845	0.0	0.0	0.0	0.0
3	0.08580220084375047	0.0	0.0	0.0	0.0
4	0.12520028802553307	0.0	0.0	0.0	0.0
5	0.15274867609885417	0.0	0.0	0.0	0.0
6	0.228344566761594	0.0	0.0	0.0	0.0
7	0.25978519176835135	0.0	0.0	0.0	0.0
8	0.2981021020241794	0.0	0.0	0.0	0.0
9	0.33572705904739664	0.0	0.0	0.0	0.0
10	0.3676001548245358	0.0	0.0	0.0	0.0
11	0.4027600284565767	0.0	0.0	0.0	0.0
12	0.4381361374738085	0.0	0.0	0.0	0.0
13	0.4723878224880476	0.0	0.0	0.0	0.0
14	0.5018390819510486	0.0	0.0	0.0	0.0
15	0.5351393312704478	0.0	0.0	0.0	0.0
16	0.574191441835925	0.0	0.0	0.0	0.0
17	0.6154059062533112	0.0	0.0	0.0	0.0
18	0.6484899201875193	0.0	0.0	0.0	0.0
19	0.6827416052017584	0.0	0.0	0.0	0.0
20	0.7246912699287937	0.0	0.0	0.0	0.0
21	0.7560886478585128	0.0	0.0	0.0	0.0
22	0.7818206586962304	0.0	0.0	0.0	0.0
23	0.808071634458406	0.0	0.0	0.0	0.0
24	0.8370471760739872	0.0	0.0	0.0	0.0
25	0.8676228595399809	0.0	0.0	0.0	0.0
26	0.894955012228111	0.0	0.0	0.0	0.0
27	0.9244062716911121	0.0	0.0	0.0	0.0
28	0.9535115545378077	0.0	0.0	0.0	0.0
29	0.984000743849725	0.0	0.0	0.0	0.0
30	1.015787345472788	0.0	0.0	0.0	0.0
31	1.0457143227832089	0.0	0.0	0.0	0.0
32	1.0771549477899662	0.0	0.0	0.0	0.0
33	1.1067359484840817	0.0	0.0	0.0	0.0
34	1.1373548790271137	0.0	0.0	0.0	0.0
35	1.1658547027952748	0.0	0.0	0.0	0.0
36	1.1899865717825797	0.0	0.0	0.0	0.0
37	1.2047770721296374	0.0	0.0	0.0	0.0
38	1.2193945841685425	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGCTA	100	0.0	150.22493	44
CTGGTCG	10	0.0063781403	150.22493	44
CCCGTTG	10	0.0063781403	150.22493	44
CCGACAA	10	0.0063781403	150.22493	44
TAGGCGT	15	1.0680277E-4	150.22493	44
TCGACTA	70	0.0	150.22493	44
CGTGCCC	55	0.0	150.22491	44
ACGATCA	95	0.0	150.22491	44
ACGGCCA	190	0.0	146.27164	44
CCCATTA	120	0.0	143.96556	44
TTCACGG	200	0.0	142.71368	44
ACGCGGA	85	0.0	141.38817	44
TATTGAC	80	0.0	140.83586	44
TCTATAT	80	0.0	140.83586	44
TTAGCTA	75	0.0	140.20995	44
TTAGATT	170	0.0	136.96979	44
ACGTCTT	55	0.0	136.5681	44
TTGGACC	50	0.0	135.20244	44
CCGACTT	35	1.4861143E-9	128.76422	44
GACGCCC	70	0.0	128.76422	44
>>END_MODULE