FastQCFastQC Report
Tue 24 May 2016
ERR841131.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841131.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15764226
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG674620.4279436237465766No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG479560.3042077676379418No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA461200.292561144454539No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC359930.22832075612212108No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG348630.2211526274743841No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC293500.18618104054077886No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG246660.15646819577440718No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT245240.15556742208593052No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCT229070.14531002029531928No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAA228920.1452148681451281No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGA220750.14003224769804748No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC213960.13572502703272588No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA211130.13392982313245191No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA204490.12971775461732152No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGA201640.12790986376368876No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA189880.12044993518869876No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA173840.1102749985949199No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA167620.10632935610032487No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAAC163400.1036524089416125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGC600.0165.9773744
CGGTACT1150.0163.5719144
CGACCCG1400.0118.5552744
ACGAACA3350.0115.6061344
ACGGTGC2700.0102.4551744
CATGACC23900.099.0771444
GACGCGT1750.097.7875343
ACATTGG30250.088.5212644
GTTCGGC3500.085.56409543
CTATCAT114300.083.9325744
CGAATGG12100.080.4373243
CCAGAAT88500.079.8941944
ACGCGGA1650.077.7855443
ACTCCGA2300.074.4035643
CGGGCAC653.6379788E-1274.0458543
TCGCACT2250.073.7677244
CGATACG453.6379788E-1273.58996642
TCCGGGC34650.072.8467743
ACGAATT1400.070.96175442
ACTTCTA91100.070.690744
Produced by FastQC (version 0.11.5)