##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841131.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15764226
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.53709240149183	39.0	37.0	39.0	35.0	39.0
2	38.01015673081571	39.0	38.0	39.0	35.0	39.0
3	38.1311333648731	39.0	38.0	39.0	35.0	39.0
4	38.63640752168866	39.0	38.0	41.0	35.0	41.0
5	39.28600072087269	40.0	39.0	41.0	36.0	41.0
6	39.33748590003721	40.0	39.0	41.0	36.0	41.0
7	39.342724025905234	40.0	39.0	41.0	36.0	41.0
8	39.2778914740248	40.0	39.0	41.0	36.0	41.0
9	39.27159519281188	40.0	39.0	41.0	36.0	41.0
10	39.209109790737585	40.0	39.0	41.0	35.0	41.0
11	39.10878085609785	40.0	38.0	41.0	35.0	41.0
12	39.053195126738224	40.0	38.0	41.0	35.0	41.0
13	39.01952477717587	40.0	38.0	41.0	35.0	41.0
14	38.985217923163496	40.0	38.0	41.0	35.0	41.0
15	38.93787186253229	40.0	38.0	41.0	35.0	41.0
16	39.01675293160603	40.0	38.0	41.0	35.0	41.0
17	39.03644378100137	40.0	38.0	41.0	35.0	41.0
18	39.04943623619707	40.0	38.0	41.0	35.0	41.0
19	39.09129233493608	40.0	39.0	41.0	35.0	41.0
20	39.12683356607549	40.0	39.0	41.0	35.0	41.0
21	39.15194859550986	40.0	39.0	41.0	35.0	41.0
22	39.13576524467487	40.0	38.0	41.0	35.0	41.0
23	39.090771725805	40.0	38.0	41.0	35.0	41.0
24	39.07208511220278	40.0	38.0	41.0	35.0	41.0
25	39.061778021804116	40.0	38.0	41.0	35.0	41.0
26	39.05549010494914	40.0	38.0	41.0	35.0	41.0
27	38.97022312573802	40.0	38.0	41.0	35.0	41.0
28	38.95419866888004	40.0	38.0	41.0	35.0	41.0
29	38.9122873054201	40.0	38.0	41.0	35.0	41.0
30	38.93707000569835	40.0	38.0	41.0	35.0	41.0
31	38.82004842699278	40.0	38.0	41.0	35.0	41.0
32	38.690037739091444	40.0	38.0	41.0	35.0	41.0
33	38.48439217889111	40.0	38.0	41.0	34.0	41.0
34	38.56819986231028	40.0	37.0	41.0	35.0	41.0
35	38.596832374635326	40.0	37.0	41.0	35.0	41.0
36	38.60029795375895	40.0	37.0	41.0	35.0	41.0
37	38.526865697636616	40.0	37.0	41.0	35.0	41.0
38	38.4790583904131	40.0	37.0	41.0	34.0	41.0
39	38.504784711453816	40.0	37.0	41.0	35.0	41.0
40	38.39004968556563	40.0	37.0	41.0	34.0	41.0
41	38.32276291700031	40.0	36.0	41.0	34.0	41.0
42	38.1939039437485	40.0	36.0	41.0	34.0	41.0
43	38.100147696853526	40.0	36.0	41.0	34.0	41.0
44	37.94353546168986	39.0	36.0	41.0	34.0	41.0
45	37.79094259803047	39.0	35.0	41.0	34.0	41.0
46	37.506704775239506	39.0	35.0	41.0	33.0	41.0
47	37.24749496928362	39.0	35.0	41.0	32.0	41.0
48	37.26925940552733	39.0	35.0	41.0	33.0	41.0
49	37.246245246294556	38.0	35.0	40.0	33.0	41.0
50	36.98453710188971	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	7.0
20	19.0
21	69.0
22	169.0
23	328.0
24	777.0
25	1668.0
26	3432.0
27	7207.0
28	15224.0
29	31403.0
30	61181.0
31	113126.0
32	196702.0
33	343426.0
34	677222.0
35	574994.0
36	882130.0
37	1443088.0
38	2231142.0
39	3625707.0
40	5555202.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	53.89917018444166	19.611010397846364	26.489819417711974
2	27.866709091838697	22.778682569001486	29.459188164391957	19.895420174767857
3	25.597913909633114	21.62734155168798	27.801580616771165	24.973163921907744
4	21.773660184775327	23.009394815831744	32.037348360775844	23.17959663861708
5	19.09526671338003	24.852365095501675	30.91546010568486	25.136908085433436
6	23.981082230107585	24.925353138175005	28.925308480099183	22.168256151618227
7	25.12214681520044	25.05179765882575	27.087248051379113	22.738807474594694
8	21.334387111679316	24.603072805477414	27.361597074287058	26.70094300855621
9	22.881795782425346	26.478122046715136	26.863596094093044	23.77648607676647
10	26.21097921331501	22.903338229228634	28.08461385925322	22.801068698203135
11	24.534702813826698	23.96221038698633	28.13808936766068	23.364997431526294
12	24.10419008202496	23.791602581693514	27.63903537033788	24.46517196594365
13	25.279300106456226	25.00829409575833	26.53074118577087	23.181664612014572
14	22.744878181776894	26.882207854670444	25.476360209502197	24.89655375405047
15	21.469097182443335	28.147731452213385	27.584919170785803	22.798252194557474
16	25.82533389206676	29.32519490649271	22.886147407427423	21.963323794013103
17	24.65826105258831	30.992482599526294	22.257020420793257	22.092235927092137
18	23.094238816418898	28.544896527111447	23.6804014355034	24.680463220966256
19	27.19913429305061	24.83360109148397	25.0858621285942	22.881402486871224
20	27.63496285830969	24.739216501970983	25.342189334255927	22.283631305463395
21	24.511669650003746	26.548978681224185	25.512086670160656	23.427264998611413
22	26.34035442019164	28.392608682468772	22.959135450100753	22.30790144723883
23	27.159328976887288	26.08716723548622	25.992173672212004	20.761330115414484
24	23.440909816948828	28.632043209733226	23.967500846536964	23.959546126780978
25	22.879327122724362	27.535939553278048	29.117278623297317	20.467454700700273
26	25.322778151948743	29.624357150581965	25.652465018496677	19.40039967897261
27	26.375871419370956	26.54534912317088	25.487664162663666	21.591115294794502
28	26.537364816196497	27.66554449157465	24.4458206292614	21.351270062967448
29	26.595615367946557	28.41261273691293	25.01450310157726	19.97726879356325
30	27.978622110519847	28.969929220124328	23.74787290443327	19.303575764922552
31	26.128390060865936	26.840307456565192	25.32599502336718	21.70530745920168
32	23.966216062004346	26.745201389271145	28.143038003573867	21.145544545150642
33	23.496157671424246	25.385766042360203	29.606334893911473	21.511741392304078
34	21.921521251194427	27.139977983637877	26.788318419233704	24.15018234593399
35	22.50634392029504	25.688879561842903	27.50993559751197	24.294840920350087
36	25.272282855372463	24.303356458556237	27.69018914283078	22.73417154324052
37	20.448350158098414	27.97294511439506	26.530009555501604	25.048695172004926
38	22.52851474016737	27.082080113724423	27.486316116479248	22.903089029628955
39	24.31987208075995	27.20341437435293	26.02729044670431	22.44942309818281
40	20.082537001975698	27.16097625577979	28.433081946106327	24.32340479613819
41	22.265927762864088	28.73478127436213	26.98795342225107	22.011337540522714
42	22.099362262392866	29.645177690292577	25.875302233895315	22.380157813419242
43	21.88844151402593	26.423756217718463	27.314951254625587	24.37285101363002
44	21.27269187789468	29.212681828612634	24.681465302397886	24.8331609910948
45	21.495969760673958	26.235886852552554	27.678775485117534	24.589367901655955
46	21.598971518182395	27.928494978864855	27.167816881923816	23.304716621028934
47	23.324933973186074	26.49194614218464	28.38324421406937	21.799875670559917
48	23.082264218799185	28.765870759184192	25.568141122536197	22.583723899480425
49	20.878870789702273	29.099252252004938	25.61779954063471	24.404077417658076
50	22.177126769792324	28.77525068229478	26.72155827481717	22.326064273095728
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	171251.0
1	173633.0
2	135460.5
3	93354.0
4	87884.5
5	60281.0
6	25013.5
7	15236.5
8	18202.0
9	20116.5
10	21455.0
11	21888.5
12	21384.5
13	22511.5
14	28676.5
15	36255.5
16	40351.5
17	42713.0
18	44127.5
19	43156.5
20	42689.5
21	50747.5
22	63883.0
23	80451.5
24	98820.0
25	117652.0
26	133694.0
27	151231.0
28	178560.5
29	211854.5
30	250994.0
31	314302.0
32	407743.5
33	452181.5
34	495372.5
35	599180.0
36	635059.0
37	628868.0
38	679166.5
39	751877.5
40	822618.5
41	888057.0
42	978912.0
43	1126627.0
44	1249311.0
45	1272974.5
46	1305817.0
47	1361338.5
48	1445136.5
49	1627002.5
50	1812706.0
51	1803311.5
52	1657249.5
53	1492338.5
54	1263340.5
55	1105524.0
56	1015813.0
57	891972.5
58	758903.0
59	642876.5
60	542913.5
61	438912.5
62	348088.5
63	272834.0
64	222802.0
65	196771.0
66	138950.5
67	80650.0
68	53041.5
69	36653.5
70	25918.5
71	18451.0
72	13724.0
73	9622.0
74	6521.5
75	4603.0
76	3527.5
77	2131.0
78	1112.0
79	749.0
80	566.5
81	355.5
82	250.0
83	275.5
84	276.5
85	223.5
86	141.5
87	100.5
88	76.0
89	68.5
90	37.5
91	4.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	68288.0
25	59889.0
26	62319.0
27	80010.0
28	84947.0
29	81535.0
30	155534.0
31	274684.0
32	270603.0
33	47260.0
34	45557.0
35	54172.0
36	67835.0
37	93472.0
38	44115.0
39	48144.0
40	51712.0
41	59254.0
42	73974.0
43	135451.0
44	498823.0
45	1219364.0
46	1681325.0
47	1919492.0
48	2685281.0
49	3044283.0
50	2856903.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.939549898497697
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.54776104080536	6.813338912953813
2	12.888446324486475	2.562107107126851
3	5.341184875064806	1.5926692074842346
4	2.800073079090255	1.1132586435623073
5	1.691504290698144	0.8406395650458578
6	1.1361257378159082	0.6775547077193239
7	0.8031240329289424	0.5587883979985951
8	0.6111183850177323	0.48593933454184624
9	0.4990761541870818	0.44645331039235464
>10	3.7151790962360343	7.212772890511621
>50	0.4406045559260606	3.090912995430759
>100	1.1812352982720025	32.5682481884557
>500	0.2686407656937106	16.939899003845035
>1k	0.06701880154674675	12.142014129044288
>5k	0.004241696300427009	2.9240169791701485
>10k+	0.004665865930469711	10.031386626717257
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	67462	0.4279436237465766	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	47956	0.3042077676379418	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	46120	0.292561144454539	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC	35993	0.22832075612212108	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG	34863	0.2211526274743841	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	29350	0.18618104054077886	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG	24666	0.15646819577440718	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	24524	0.15556742208593052	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCT	22907	0.14531002029531928	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAA	22892	0.1452148681451281	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGA	22075	0.14003224769804748	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	21396	0.13572502703272588	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	21113	0.13392982313245191	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	20449	0.12971775461732152	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGA	20164	0.12790986376368876	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	18988	0.12044993518869876	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	17384	0.1102749985949199	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16762	0.10632935610032487	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAAC	16340	0.1036524089416125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.057414807425369314	0.0	0.0	0.0	0.0
2	0.0881045476003706	0.0	0.0	0.0	0.0
3	0.11244446761927925	0.0	0.0	0.0	0.0
4	0.1512982622806854	0.0	0.0	0.0	0.0
5	0.17525123022214983	0.0	0.0	0.0	0.0
6	0.2437607783598129	0.0	0.0	0.0	0.0
7	0.27573824430073507	0.0	0.0	0.0	0.0
8	0.3190895639278452	0.0	0.0	0.0	0.0
9	0.36209833581426704	0.0	0.0	0.0	0.0
10	0.39397430612831863	0.0	0.0	0.0	0.0
11	0.4349024176638929	0.0	0.0	0.0	0.0
12	0.4680788006972242	0.0	0.0	0.0	0.0
13	0.5063807128875214	0.0	0.0	0.0	0.0
14	0.5434963949387683	0.0	0.0	0.0	0.0
15	0.58575029310034	0.0	0.0	0.0	0.0
16	0.6211278625414277	0.0	0.0	0.0	0.0
17	0.6662299817320558	0.0	0.0	0.0	0.0
18	0.7005164731842844	0.0	0.0	0.0	0.0
19	0.7344667603725041	0.0	0.0	0.0	0.0
20	0.7749444850638401	0.0	0.0	0.0	0.0
21	0.8063320076735768	0.0	0.0	0.0	0.0
22	0.8370724956620135	0.0	0.0	0.0	0.0
23	0.8677051445469001	0.0	0.0	0.0	0.0
24	0.8992956584103781	0.0	0.0	0.0	0.0
25	0.930962293994009	0.0	0.0	0.0	0.0
26	0.9629905077483665	0.0	0.0	0.0	0.0
27	0.9907685921275171	0.0	0.0	0.0	0.0
28	1.0215090801159536	0.0	0.0	0.0	0.0
29	1.0522685985344284	0.0	0.0	0.0	0.0
30	1.0832818560200799	0.0	0.0	0.0	0.0
31	1.1107554535186186	0.0	0.0	0.0	0.0
32	1.1397197680368196	0.0	0.0	0.0	0.0
33	1.169153499829297	0.0	0.0	0.0	0.0
34	1.1982193099743685	0.0	0.0	0.0	0.0
35	1.2276466982901666	0.0	0.0	0.0	0.0
36	1.2507242664498721	0.0	0.0	0.0	0.0
37	1.2670016276092464	0.0	0.0	0.0	0.0
38	1.280747941573535	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGCGC	60	0.0	165.97737	44
CGGTACT	115	0.0	163.57191	44
CGACCCG	140	0.0	118.55527	44
ACGAACA	335	0.0	115.60613	44
ACGGTGC	270	0.0	102.45517	44
CATGACC	2390	0.0	99.07714	44
GACGCGT	175	0.0	97.78753	43
ACATTGG	3025	0.0	88.52126	44
GTTCGGC	350	0.0	85.564095	43
CTATCAT	11430	0.0	83.93257	44
CGAATGG	1210	0.0	80.43732	43
CCAGAAT	8850	0.0	79.89419	44
ACGCGGA	165	0.0	77.78554	43
ACTCCGA	230	0.0	74.40356	43
CGGGCAC	65	3.6379788E-12	74.04585	43
TCGCACT	225	0.0	73.76772	44
CGATACG	45	3.6379788E-12	73.589966	42
TCCGGGC	3465	0.0	72.84677	43
ACGAATT	140	0.0	70.961754	42
ACTTCTA	9110	0.0	70.6907	44
>>END_MODULE