FastQCFastQC Report
Tue 24 May 2016
ERR841130.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841130.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2918164
Sequences flagged as poor quality0
Sequence length24-50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC243060.832920973598468No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG171230.5867730531937204No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA144970.49678496479293144No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA138310.47396239553363007No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT117890.40398689038724345No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC93800.3214349844628335No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA92820.3180767085057591No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC89290.3059800614358892No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA87230.29892082830163075No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA82100.2813412817100067No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT80410.27554996909015395No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT71860.24625072477078053No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC65710.22517582973403824No Hit
CTCTTCCCTTTTGCGGCCATCACCGAAGCGGGAGCGGCCAAAATGAAGT61950.21229101585791615No Hit
CTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTTGA57960.19861803517554188No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA56450.19344354875188646No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA55320.18957125096464764No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT54370.18631577937360616No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA52580.18018178553364375No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA51080.17504156723199932No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA46710.16006639791320845No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG46260.1585243324227151No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATT46160.15818165120260547No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGA44410.15218472985068693No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA42860.14687317093898766No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA42760.14653048971887803No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAGGA41570.14245258319957343No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA40210.13779211860608245No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG40080.13734663301993993No Hit
AAGCTCTGTTTGGTGCTTTGGATCCATTTCCATCGGTCCTTACAGCCG39630.13580456752944658No Hit
CTCTTTCCCATCTTGCAAGATGGCGGG38220.1309727623259008No Hit
CTCTTCCTTTCTCCGCCATCGTGGTGTGTTCTTGACTCCGCTGCTCGCC36170.12394779731365337No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA34620.1186362384019541No Hit
AGGGGAAAGAGGAGCAACAAAGAAAGCTTTGAAAGAGCAGCCATTGACT33560.11500381746879203No Hit
AAAAAAAAAAAAAAAAAAAAAAAA33060.11329041136824386No Hit
CCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAA32730.11215956334188208No Hit
TTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTACC32700.11205675897584921No Hit
CTCTTCCCTTTTGCGGCCATCACCGAAGCGGGAGCGGCCAAAATGAA31770.10886982362882963No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGA31400.10760190311442401No Hit
AGACGGAAGTGAGCGACACACTCTGCGTCCTCGCCTCACCAGGAAACTA31230.10701934504023763No Hit
CTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTT30620.10492898959756887No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGAC30550.10468911274349214No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30500.10451777213343733No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCG29300.10040559749212176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACG350.0276.2192444
ATTATCC100.0010305861276.2192444
GCCAATT400.0276.2192444
CCCGTAC159.41014E-6276.219244
ATCCACG600.0276.219244
CCGGGCT2450.0270.582144
GTTAATC900.0260.8737544
GTGCATT650.0254.9716244
CCCATTG1200.0253.2009644
TCCCTCA500.0248.597344
TGCGTAT500.0248.597344
ATGGGGC950.0247.1435244
CCGTGTT1450.0228.5952544
ATAACTC1150.0228.181144
TATGGAC850.0227.4746744
ATGTATG1100.0213.4421444
ATCAAGT1450.0209.5456444
TTGTACG405.4569682E-11207.1644144
AGATGTA800.0207.1644144
TGCAACT405.4569682E-11207.1644144
Produced by FastQC (version 0.11.5)