##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841130.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2918164
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.284893172556444	39.0	37.0	39.0	34.0	39.0
2	37.88850797967489	39.0	38.0	39.0	35.0	39.0
3	38.049284413076165	39.0	38.0	39.0	35.0	39.0
4	38.78390864941107	39.0	38.0	41.0	35.0	41.0
5	39.25325547159104	40.0	39.0	41.0	35.0	41.0
6	39.20652334824225	40.0	38.0	41.0	35.0	41.0
7	39.15219706637461	40.0	38.0	41.0	35.0	41.0
8	39.10618286018195	40.0	38.0	41.0	35.0	41.0
9	39.031373836425914	40.0	38.0	41.0	35.0	41.0
10	39.01563928552336	40.0	38.0	41.0	35.0	41.0
11	38.888682061734706	40.0	38.0	41.0	35.0	41.0
12	38.832824337494394	40.0	38.0	41.0	35.0	41.0
13	38.69560072703248	40.0	38.0	41.0	35.0	41.0
14	38.6545348376582	40.0	38.0	41.0	35.0	41.0
15	38.54250994803582	40.0	38.0	41.0	35.0	41.0
16	38.68789451175465	40.0	38.0	41.0	35.0	41.0
17	38.77077504896915	40.0	38.0	41.0	35.0	41.0
18	38.80817424928825	40.0	38.0	41.0	35.0	41.0
19	38.80522924688263	40.0	38.0	41.0	35.0	41.0
20	38.81592535580591	40.0	38.0	41.0	35.0	41.0
21	38.89963209744209	40.0	38.0	41.0	35.0	41.0
22	38.94877429781191	40.0	38.0	41.0	35.0	41.0
23	38.89040883240284	40.0	38.0	41.0	35.0	41.0
24	38.829346123110284	40.0	38.0	41.0	35.0	41.0
25	38.77162434157881	40.0	38.0	41.0	35.0	41.0
26	38.69607313655425	40.0	38.0	41.0	35.0	41.0
27	38.6605641651944	40.0	38.0	41.0	35.0	41.0
28	38.4944749992353	40.0	38.0	41.0	34.0	41.0
29	38.44747734249301	40.0	37.0	41.0	34.0	41.0
30	38.44978229307477	40.0	37.0	41.0	34.0	41.0
31	38.39100832029594	40.0	37.0	41.0	34.0	41.0
32	38.20006338876642	40.0	37.0	41.0	34.0	41.0
33	38.18477893689512	40.0	37.0	41.0	34.0	41.0
34	38.12238972602973	40.0	37.0	41.0	34.0	41.0
35	38.13734866370637	40.0	37.0	41.0	34.0	41.0
36	38.138154180553435	40.0	36.0	41.0	34.0	41.0
37	38.0881833654289	39.0	36.0	41.0	34.0	41.0
38	37.92680882681306	39.0	36.0	41.0	34.0	41.0
39	37.92348541467513	39.0	36.0	41.0	34.0	41.0
40	37.86479332245727	39.0	36.0	41.0	34.0	41.0
41	37.73832239651194	39.0	35.0	41.0	34.0	41.0
42	37.6140911405594	39.0	35.0	41.0	34.0	41.0
43	37.45966205696853	39.0	35.0	41.0	33.0	41.0
44	37.260434929593615	38.0	35.0	41.0	33.0	41.0
45	36.99925592312916	38.0	35.0	40.0	33.0	41.0
46	36.78108221366043	37.0	35.0	40.0	32.0	41.0
47	36.45837402460244	37.0	35.0	40.0	31.0	41.0
48	36.573391698595664	37.0	35.0	40.0	32.0	41.0
49	36.253525225535064	37.0	34.0	40.0	31.0	41.0
50	36.580865641512794	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	9.0
21	33.0
22	67.0
23	128.0
24	251.0
25	437.0
26	818.0
27	1666.0
28	3547.0
29	7204.0
30	14396.0
31	26791.0
32	45935.0
33	80981.0
34	137725.0
35	127831.0
36	206984.0
37	348177.0
38	479622.0
39	685088.0
40	750473.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	42.474891747002566	22.895834504160835	34.629273748836596
2	23.443404825774014	18.941978586535917	33.62377851279092	23.99083807489915
3	19.32958531460192	21.935333312315553	32.58990927172016	26.145172101362363
4	19.949324301170186	20.214456761169007	39.43133422247687	20.40488471518393
5	16.823523283818183	22.48752983040021	37.35300689063397	23.335939995147633
6	22.562508481360197	21.485427138433618	30.603043557524522	25.349020822681656
7	24.829824506093555	24.384475992439082	23.698291117291557	27.087408384175802
8	26.333406895568583	23.4164700818734	28.251188075790118	21.998934946767896
9	23.287998892454294	23.958317627110745	29.610330330988937	23.14335314944602
10	23.12937175566555	22.123396765911718	27.806319315843798	26.94091216257894
11	27.874444342401596	20.67748762578114	26.587505020279874	24.860563011537394
12	27.249119651945534	25.964579098364588	24.74374298360202	22.042558266087855
13	26.98631742424346	23.775874145524377	24.001461192722548	25.23634723750961
14	22.739366259058777	24.778559395565157	25.74002009482675	26.74205425054932
15	23.829880705813654	29.657723143730095	25.608944528134813	20.90345162232143
16	23.47959196261759	27.22036869757834	25.086458471833662	24.213580867970407
17	24.874887086537974	27.994005820097843	24.373133244053452	22.75797384931073
18	22.787958456070324	24.534159149382965	24.712558992572042	27.96532340197467
19	26.01810590494571	21.492452103445867	23.839441511854716	28.650000479753707
20	22.95155447055066	24.256553092972155	25.775624673596138	27.016267762881046
21	23.710010815019306	27.28537532503314	26.30225717266062	22.702356687286937
22	26.371170366024664	24.066947573885496	23.209250748073103	26.352631312016733
23	27.40617045512178	26.04082566983898	24.448591648721592	22.104412226317642
24	26.961884253249647	26.219979411712295	24.923684892281585	21.894451442756473
25	27.790911849841905	24.73800081089451	25.556818248364515	21.91426909089907
26	25.84524302349952	30.36958001562362	22.46434021384613	21.320836747030732
27	28.835121560629656	25.88637741163079	23.993376712056463	21.285124315683092
28	26.617674306751194	24.7334888984911	25.638260731376565	23.01057606338114
29	26.377124728503464	24.42405152601793	26.296783736657403	22.9020400088212
30	28.003951679141952	25.696601398671383	25.238741324427284	21.06070559775938
31	27.59844622200095	28.361550073014254	23.82254570084274	20.21745800414206
32	23.680554681327045	27.015536912478332	27.88768784290842	21.416220563286203
33	25.67428380118349	24.238402665086976	29.5814938240315	20.505819709698034
34	22.14393527674057	27.63740966272698	27.79719823502649	22.421456825505963
35	24.339049501175523	24.72799246266364	25.763502780361154	25.169455255799683
36	23.267897965197285	23.87156487189232	27.344784300390995	25.5157528625194
37	22.28031268463252	28.901028922792094	25.459191375654523	23.359467016920867
38	25.76333761943993	26.1106662457556	24.511922819282862	23.61407331552161
39	21.355812404043913	29.333526452497516	25.338817917596053	23.971843225862518
40	22.583401470206425	26.764770846976855	24.59181815011296	26.06000953270376
41	20.60416228175799	29.16585128438757	28.014537990459736	22.215448443394703
42	23.66330856016001	26.92473163699283	24.586142511062665	24.825817291784492
43	22.692204410095922	25.422310665987325	27.53266307519181	24.352821848724936
44	23.188214780183877	25.9289728349186	24.565084343115988	26.31772804178153
45	20.96649709052701	26.157778231157952	30.17740729352591	22.69831738478913
46	22.38863757434519	26.580382434460958	26.740981437324113	24.289998553869736
47	23.075846404906493	28.2204952511831	26.354210737829465	22.349447606080947
48	26.004235711858787	28.68029507628713	22.937104916460857	22.378364295393226
49	19.308617385947475	29.575801325053952	24.266231532958166	26.849349756040407
50	19.683666805200975	30.590749866413347	27.835658730629937	21.889924597755744
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	48581.0
1	51439.5
2	46713.0
3	37913.5
4	33279.0
5	20338.5
6	6969.5
7	3493.5
8	4303.0
9	4664.5
10	4397.0
11	4088.0
12	3918.5
13	3968.0
14	4678.0
15	5439.0
16	5958.5
17	6032.0
18	5640.0
19	6442.0
20	8637.0
21	10990.0
22	12644.0
23	16052.0
24	19448.0
25	21510.5
26	23737.5
27	26580.0
28	29318.5
29	29482.5
30	31367.0
31	36132.5
32	43388.0
33	53306.0
34	76254.5
35	111085.5
36	123523.5
37	109121.5
38	100785.5
39	100204.0
40	100645.0
41	117475.0
42	138641.5
43	146892.5
44	160653.0
45	180420.5
46	201592.5
47	222438.5
48	250698.0
49	275216.0
50	302759.0
51	319009.0
52	303691.0
53	288694.0
54	272904.5
55	272293.5
56	278121.0
57	263846.0
58	242773.0
59	206294.5
60	148979.0
61	108861.5
62	91835.5
63	71290.5
64	51408.0
65	42618.5
66	31974.0
67	21614.0
68	15716.5
69	10844.5
70	8452.0
71	6320.0
72	4030.0
73	2489.5
74	1451.0
75	1150.5
76	866.0
77	594.0
78	389.0
79	296.5
80	206.0
81	108.0
82	20.0
83	9.0
84	3.5
85	1.0
86	1.0
87	1.0
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	15197.0
25	16312.0
26	19197.0
27	23205.0
28	23775.0
29	22680.0
30	33954.0
31	63050.0
32	45421.0
33	11254.0
34	10828.0
35	9990.0
36	13387.0
37	12374.0
38	8465.0
39	8485.0
40	10221.0
41	13571.0
42	17080.0
43	28369.0
44	94932.0
45	252020.0
46	278243.0
47	396912.0
48	430870.0
49	637297.0
50	421075.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.629021755186454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.98224401064192	4.639138180233123
2	11.17021921020622	1.4809525230931737
3	4.656147844110611	0.9259711606192123
4	2.559640562283266	0.6787165189133383
5	1.595254950435023	0.528748988575133
6	1.0409048054767926	0.414010836035027
7	0.7451028053050548	0.34575118943523653
8	0.6268791748506061	0.3324476550366401
9	0.46781465415137075	0.27910381677880175
>10	4.008860993187664	5.588639311155235
>50	0.7871447211975494	3.7499318952029466
>100	1.5737401439154937	26.944940950256964
>500	0.571270418733722	26.640745781612384
>1k	0.20378973591366425	20.647511066839673
>5k	0.008239477193274836	3.834046192493849
>10k+	0.0027464923977582787	2.9693439337192733
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	24306	0.832920973598468	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	17123	0.5867730531937204	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	14497	0.49678496479293144	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	13831	0.47396239553363007	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	11789	0.40398689038724345	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	9380	0.3214349844628335	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA	9282	0.3180767085057591	No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC	8929	0.3059800614358892	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	8723	0.29892082830163075	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	8210	0.2813412817100067	No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT	8041	0.27554996909015395	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	7186	0.24625072477078053	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	6571	0.22517582973403824	No Hit
CTCTTCCCTTTTGCGGCCATCACCGAAGCGGGAGCGGCCAAAATGAAGT	6195	0.21229101585791615	No Hit
CTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTTGA	5796	0.19861803517554188	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA	5645	0.19344354875188646	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	5532	0.18957125096464764	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT	5437	0.18631577937360616	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	5258	0.18018178553364375	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	5108	0.17504156723199932	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA	4671	0.16006639791320845	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	4626	0.1585243324227151	No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATT	4616	0.15818165120260547	No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGA	4441	0.15218472985068693	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	4286	0.14687317093898766	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	4276	0.14653048971887803	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAGGA	4157	0.14245258319957343	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4021	0.13779211860608245	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG	4008	0.13734663301993993	No Hit
AAGCTCTGTTTGGTGCTTTGGATCCATTTCCATCGGTCCTTACAGCCG	3963	0.13580456752944658	No Hit
CTCTTTCCCATCTTGCAAGATGGCGGG	3822	0.1309727623259008	No Hit
CTCTTCCTTTCTCCGCCATCGTGGTGTGTTCTTGACTCCGCTGCTCGCC	3617	0.12394779731365337	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	3462	0.1186362384019541	No Hit
AGGGGAAAGAGGAGCAACAAAGAAAGCTTTGAAAGAGCAGCCATTGACT	3356	0.11500381746879203	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	3306	0.11329041136824386	No Hit
CCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAA	3273	0.11215956334188208	No Hit
TTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTACC	3270	0.11205675897584921	No Hit
CTCTTCCCTTTTGCGGCCATCACCGAAGCGGGAGCGGCCAAAATGAA	3177	0.10886982362882963	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGA	3140	0.10760190311442401	No Hit
AGACGGAAGTGAGCGACACACTCTGCGTCCTCGCCTCACCAGGAAACTA	3123	0.10701934504023763	No Hit
CTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTT	3062	0.10492898959756887	No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGAC	3055	0.10468911274349214	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3050	0.10451777213343733	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCG	2930	0.10040559749212176	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.06356736633033647	0.0	0.0	0.0	0.0
2	0.0990691407336942	0.0	0.0	0.0	0.0
3	0.13045874049573636	0.0	0.0	0.0	0.0
4	0.17908520562929295	0.0	0.0	0.0	0.0
5	0.20965237046307197	0.0	0.0	0.0	0.0
6	0.27757178828880075	0.0	0.0	0.0	0.0
7	0.31393026574243255	0.0	0.0	0.0	0.0
8	0.35560030210776367	0.0	0.0	0.0	0.0
9	0.39888094020761	0.0	0.0	0.0	0.0
10	0.4344855189770006	0.0	0.0	0.0	0.0
11	0.4696788802822597	0.0	0.0	0.0	0.0
12	0.5122398878198758	0.0	0.0	0.0	0.0
13	0.547227640393069	0.0	0.0	0.0	0.0
14	0.5799879650355497	0.0	0.0	0.0	0.0
15	0.6175800948815762	0.0	0.0	0.0	3.426812201096306E-5
16	0.6538014998471642	0.0	0.0	0.0	3.426812201096306E-5
17	0.7011257763443042	0.0	0.0	0.0	3.426812201096306E-5
18	0.7311103831038969	0.0	0.0	0.0	3.426812201096306E-5
19	0.7618146204257197	0.0	0.0	0.0	3.426812201096306E-5
20	0.7981730978793515	0.0	0.0	0.0	3.426812201096306E-5
21	0.8298368426174814	0.0	0.0	0.0	3.426812201096306E-5
22	0.8545098904653748	0.0	0.0	0.0	3.426812201096306E-5
23	0.8816845112200685	0.0	0.0	0.0	3.426812201096306E-5
24	0.9098529075130801	0.0	0.0	0.0	3.426812201096306E-5
25	0.9377128907079931	0.0	0.0	0.0	3.426812201096306E-5
26	0.966532381319213	0.0	0.0	0.0	3.426812201096306E-5
27	0.9956602850285317	0.0	0.0	0.0	3.426812201096306E-5
28	1.021155767804688	0.0	0.0	0.0	3.426812201096306E-5
29	1.0524425632006975	0.0	0.0	0.0	3.426812201096306E-5
30	1.0816732712760488	0.0	0.0	0.0	3.426812201096306E-5
31	1.1083338702005783	0.0	0.0	0.0	3.426812201096306E-5
32	1.1358511721753815	0.0	0.0	0.0	3.426812201096306E-5
33	1.1629572566860533	0.0	0.0	0.0	3.426812201096306E-5
34	1.1908172398809662	0.0	0.0	0.0	3.426812201096306E-5
35	1.2154560196068487	0.0	0.0	0.0	3.426812201096306E-5
36	1.2376617626699526	0.0	0.0	0.0	3.426812201096306E-5
37	1.2535279031610287	0.0	0.0	0.0	3.426812201096306E-5
38	1.2686401449678633	0.0	0.0	0.0	3.426812201096306E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGACG	35	0.0	276.21924	44
ATTATCC	10	0.0010305861	276.21924	44
GCCAATT	40	0.0	276.21924	44
CCCGTAC	15	9.41014E-6	276.2192	44
ATCCACG	60	0.0	276.2192	44
CCGGGCT	245	0.0	270.5821	44
GTTAATC	90	0.0	260.87375	44
GTGCATT	65	0.0	254.97162	44
CCCATTG	120	0.0	253.20096	44
TCCCTCA	50	0.0	248.5973	44
TGCGTAT	50	0.0	248.5973	44
ATGGGGC	95	0.0	247.14352	44
CCGTGTT	145	0.0	228.59525	44
ATAACTC	115	0.0	228.1811	44
TATGGAC	85	0.0	227.47467	44
ATGTATG	110	0.0	213.44214	44
ATCAAGT	145	0.0	209.54564	44
TTGTACG	40	5.4569682E-11	207.16441	44
AGATGTA	80	0.0	207.16441	44
TGCAACT	40	5.4569682E-11	207.16441	44
>>END_MODULE