FastQCFastQC Report
Tue 24 May 2016
ERR841129.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841129.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8211780
Sequences flagged as poor quality0
Sequence length24-50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA477750.5817861657277715No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT248610.30274800347793046No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC247580.3014937078197419No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG186060.22657694190540903No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC156360.19040938748967948No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA133470.1625347975712939No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA126500.1540469910299594No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT124600.15173324175757266No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT123530.15043023558838645No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117310.1428557511282572No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG115270.14037151506737883No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT107810.1312870047663235No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC106390.12955778162590814No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA89820.10937945244514588No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88490.10775982795447517No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGA85030.10354636875318139No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA82750.10076986962631732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG850.0133.0350244
GTACCGA800.0131.253344
CAACGAA309.354488E-6107.6950144
GGCCGAC1650.0102.79977444
AATACGG1450.094.6973444
CGCATTC950.090.70058443
CCGATAA850.089.4452143
TATTAGG2950.087.62598443
GATATGC2900.086.3416944
CTATCAT91200.082.8082544
TACGATC1350.077.7797344
AACCGCT951.8189894E-1276.52013444
GCGCCAA1850.073.973643
GTTCGGC7600.069.3592743
ACACGTC1400.069.05231542
ATACCGG401.5279511E-1069.0523142
CGTAGCG1400.068.7876343
GAACACA57050.068.6661844
AACGACA32400.067.7372643
CGAATTA2350.067.5831142
Produced by FastQC (version 0.11.5)