##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841129.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8211780
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.729515403481344	39.0	38.0	39.0	35.0	39.0
2	38.059515476547105	39.0	38.0	39.0	35.0	39.0
3	38.18383419916267	39.0	38.0	39.0	35.0	39.0
4	38.623683537552154	39.0	38.0	40.0	35.0	41.0
5	39.433906777824056	41.0	39.0	41.0	36.0	41.0
6	39.4398037940617	41.0	39.0	41.0	36.0	41.0
7	39.4376649155238	41.0	39.0	41.0	36.0	41.0
8	39.372919391410875	40.0	39.0	41.0	36.0	41.0
9	39.32020852969758	40.0	39.0	41.0	36.0	41.0
10	39.342610493705386	40.0	39.0	41.0	36.0	41.0
11	39.28655650784604	40.0	39.0	41.0	36.0	41.0
12	39.23756652029158	40.0	39.0	41.0	35.0	41.0
13	39.135912798443215	40.0	39.0	41.0	35.0	41.0
14	39.135679840424366	40.0	38.0	41.0	35.0	41.0
15	39.1465819834433	40.0	39.0	41.0	35.0	41.0
16	39.15812174705119	40.0	39.0	41.0	35.0	41.0
17	39.192857699548696	40.0	39.0	41.0	35.0	41.0
18	39.127930119900924	40.0	39.0	41.0	35.0	41.0
19	39.12177262420571	41.0	39.0	41.0	35.0	41.0
20	39.10980006770761	41.0	39.0	41.0	35.0	41.0
21	39.19308371631973	41.0	39.0	41.0	35.0	41.0
22	39.217303678374236	41.0	39.0	41.0	35.0	41.0
23	39.1949538346132	41.0	39.0	41.0	35.0	41.0
24	39.10612960892766	40.0	38.0	41.0	35.0	41.0
25	39.09010249699635	40.0	38.0	41.0	35.0	41.0
26	39.08358353127922	40.0	38.0	41.0	35.0	41.0
27	39.06994447904872	40.0	38.0	41.0	35.0	41.0
28	39.03013670638672	40.0	38.0	41.0	35.0	41.0
29	39.0128166703168	40.0	38.0	41.0	35.0	41.0
30	38.99516286658877	40.0	38.0	41.0	35.0	41.0
31	38.922446612161586	40.0	38.0	41.0	35.0	41.0
32	38.80751727936008	40.0	38.0	41.0	35.0	41.0
33	38.739555133476834	40.0	38.0	41.0	35.0	41.0
34	38.750730718779494	40.0	38.0	41.0	35.0	41.0
35	38.7891655463119	40.0	38.0	41.0	35.0	41.0
36	38.76387996189927	40.0	38.0	41.0	35.0	41.0
37	38.77641067197119	40.0	38.0	41.0	35.0	41.0
38	38.73403428920954	40.0	38.0	41.0	35.0	41.0
39	38.70953760594798	40.0	38.0	41.0	35.0	41.0
40	38.634807483924554	40.0	37.0	41.0	35.0	41.0
41	38.611275919713385	40.0	37.0	41.0	35.0	41.0
42	38.51218214299289	40.0	37.0	41.0	35.0	41.0
43	38.442749716435614	40.0	37.0	41.0	35.0	41.0
44	38.358197468628575	40.0	37.0	41.0	34.0	41.0
45	38.197288506731	40.0	36.0	41.0	34.0	41.0
46	37.91440140285218	40.0	36.0	41.0	34.0	41.0
47	37.69560688179136	39.0	36.0	41.0	33.0	41.0
48	37.56355542447665	39.0	36.0	41.0	33.0	41.0
49	37.69747947157342	39.0	36.0	41.0	33.0	41.0
50	37.0872294484207	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	4.0
20	18.0
21	43.0
22	105.0
23	284.0
24	510.0
25	953.0
26	1813.0
27	3702.0
28	7517.0
29	15303.0
30	29759.0
31	54857.0
32	94861.0
33	166670.0
34	312165.0
35	275697.0
36	429191.0
37	725443.0
38	1031793.0
39	1826378.0
40	3234712.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	52.46997605878384	16.676883696348415	30.85314024486774
2	25.021907552321178	22.353631003266038	32.346714110704376	20.277747333708405
3	22.39961372564779	23.518360209357777	29.037358526409623	25.044667538584815
4	22.02959650648215	23.08559167439946	33.42431239024913	21.46049942886926
5	16.823015229341262	26.495911970364528	32.30046348051214	24.380609319782067
6	20.268285316947118	28.985615786102404	28.496306525503606	22.249792371446873
7	23.02737043612956	27.133276829140573	25.39853722335474	24.44081551137512
8	21.78528893857361	26.440771671915225	29.292029255532903	22.48191013397826
9	21.953449800165128	27.493771143406182	28.677923665758215	21.874855390670476
10	25.166589947611843	22.782904559060277	29.000496847212176	23.050008646115703
11	24.836941564435485	24.80713073170494	27.27344132453621	23.082486379323363
12	23.018127616667762	25.494777015458283	28.66349317687517	22.823602190998784
13	23.52281722111406	23.72643933471184	28.844805876436048	23.905937567738054
14	19.20506881577441	26.932041530581674	27.66778944394516	26.19510020969875
15	21.5107199657078	29.16461473639089	28.223600729683458	21.101064568217854
16	21.148106744213802	31.06388627069892	26.13175219014635	21.656254794940928
17	22.840516915942707	33.132962646344644	24.541366183701953	19.485154254010702
18	20.49800408681187	27.46442306053012	25.67965775994973	26.35791509270828
19	24.049925838247006	25.222984541719335	26.317643677741003	24.409445942292656
20	22.46392377779239	24.449607758610192	28.463889680434697	24.622578783162723
21	20.999381376510332	28.64391155145413	27.3350114104372	23.021695661598336
22	23.10050926839248	26.987194006658726	25.308934238374626	24.603362486574166
23	26.51529875374158	24.698603713202253	28.004854002420913	20.78124353063526
24	22.526127100336346	28.347788177471877	25.658322556132774	23.467762166059003
25	23.371029442728673	26.88405756343611	27.215651107687993	22.529261886147218
26	23.93493285640482	29.433618327101357	27.847587053719174	18.78386176277465
27	23.843506827463052	27.318872089086227	26.494152260372484	22.343468823078236
28	23.636607631777018	26.55489998936103	25.853535060735844	23.95495731812611
29	23.314039031451113	28.4932666505336	26.50432650701258	21.688367811002713
30	25.573117569452375	28.366264100341976	26.290136965768866	19.770481364436783
31	24.809246406355268	27.693150033850394	26.013756728344667	21.483846831449675
32	21.470464192161703	27.779213693997658	28.38557445891968	22.364747654920958
33	22.164968567321615	24.96439369891069	30.79748622941795	22.07315150434974
34	23.1600195370151	29.073434556955824	26.07848691377373	21.688058992255346
35	20.97895123106658	26.717044669915797	27.55417977215019	24.749824326867436
36	20.764059375507347	27.018623025883816	28.50091799518489	23.716399603423945
37	20.992347362388404	30.49439135297846	25.031073661403937	23.482187623229194
38	23.81448475819387	27.280573474660414	25.88121930149062	23.02372246565509
39	22.748466029461454	29.14382967620273	26.213992107870336	21.89371218646548
40	19.90283085794126	28.611960934441584	27.050349734292777	24.43485847332438
41	21.01599959198002	29.931805862791343	26.240038913820428	22.812155631408206
42	21.140550319330057	29.72460873399961	26.933081351476133	22.201759595194197
43	19.762283943523535	26.962977126787695	27.768873896338363	25.505865033350407
44	22.458770291654528	27.663762427820473	25.012130345587437	24.865336934937563
45	21.38932147555667	26.820606679276853	29.676170459416973	22.1139013857495
46	21.8619743890805	29.79539700594408	26.50766859910472	21.8349600058707
47	22.579295032965796	29.289698809868774	25.92804072267394	22.20296543449149
48	23.50203299767771	28.78638778007166	24.47625332732076	23.235325894929876
49	21.150021802816553	31.09708087923696	23.472574513428693	24.28032280451779
50	19.492910446842508	29.65508751455703	27.56827957386357	23.28372246473689
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	96482.0
1	96938.5
2	113052.0
3	127143.0
4	118495.5
5	80062.0
6	36068.5
7	23703.5
8	22501.5
9	19502.0
10	17258.5
11	16095.5
12	14887.5
13	13952.5
14	14307.0
15	15161.0
16	16368.5
17	20042.5
18	25395.5
19	27945.5
20	29581.5
21	37039.5
22	45334.0
23	52895.5
24	61170.0
25	72548.0
26	86767.5
27	110741.5
28	137734.0
29	153641.0
30	174203.0
31	223017.0
32	295509.5
33	330557.5
34	344343.0
35	386160.5
36	406031.0
37	402306.5
38	406277.0
39	416089.0
40	438970.5
41	472686.5
42	514173.5
43	576847.5
44	620787.5
45	615719.0
46	636882.5
47	648211.0
48	653523.0
49	699340.0
50	790369.5
51	849165.0
52	779860.0
53	660190.0
54	568416.5
55	499811.0
56	443372.0
57	418567.0
58	398290.0
59	338884.5
60	273335.5
61	222547.0
62	171452.0
63	136998.0
64	114018.0
65	95276.0
66	64794.5
67	38501.5
68	29665.5
69	22901.5
70	16188.5
71	10779.5
72	7220.5
73	4403.0
74	2723.0
75	1902.5
76	1169.0
77	825.0
78	507.0
79	222.0
80	118.0
81	65.5
82	38.0
83	24.5
84	15.0
85	29.5
86	47.0
87	47.0
88	46.5
89	46.0
90	24.0
91	1.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	33394.0
25	35167.0
26	38891.0
27	49035.0
28	51030.0
29	51619.0
30	75335.0
31	123839.0
32	167734.0
33	30846.0
34	25281.0
35	27391.0
36	28847.0
37	31438.0
38	22136.0
39	22181.0
40	25428.0
41	31977.0
42	39400.0
43	60704.0
44	190781.0
45	508366.0
46	926201.0
47	820361.0
48	1537938.0
49	1225655.0
50	2030805.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.663438187732964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.2091739830976	6.784620129974531
2	12.089640598852768	2.3365498927784127
3	4.8467947379016785	1.4051010407502678
4	2.493657737010171	0.9638922961183939
5	1.5156479059892434	0.7323184926946978
6	1.0361737219881195	0.6007800428511236
7	0.7452036710815195	0.5040860728937566
8	0.5734840191656129	0.4433461896687653
9	0.46844241588933017	0.40740878974129563
>10	3.680362147428437	7.133091660282853
>50	0.5647287464214183	3.9254307231230765
>100	1.394691037550402	37.190006019839245
>500	0.32742795224893234	19.978828777457128
>1k	0.044797356650973334	7.416225197874407
>5k	0.006244480018014465	4.219455176576914
>10k+	0.0035294887058342625	5.958859497375145
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	47775	0.5817861657277715	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	24861	0.30274800347793046	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	24758	0.3014937078197419	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	18606	0.22657694190540903	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	15636	0.19040938748967948	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	13347	0.1625347975712939	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	12650	0.1540469910299594	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12460	0.15173324175757266	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12353	0.15043023558838645	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11731	0.1428557511282572	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	11527	0.14037151506737883	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT	10781	0.1312870047663235	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	10639	0.12955778162590814	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	8982	0.10937945244514588	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8849	0.10775982795447517	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGA	8503	0.10354636875318139	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	8275	0.10076986962631732	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.047151774645691924	0.0	0.0	0.0	0.0
2	0.0736015821173972	0.0	0.0	0.0	0.0
3	0.10097688929805718	0.0	0.0	0.0	0.0
4	0.1365720952095648	0.0	0.0	0.0	0.0
5	0.1606350876423869	0.0	0.0	0.0	0.0
6	0.22561490931320616	0.0	0.0	0.0	0.0
7	0.2544880647070428	0.0	0.0	0.0	0.0
8	0.2908382835390135	0.0	0.0	0.0	0.0
9	0.3341175725603949	0.0	0.0	0.0	0.0
10	0.36320992525372087	0.0	0.0	0.0	0.0
11	0.3975508355070399	0.0	0.0	0.0	0.0
12	0.42865249677901746	0.0	0.0	0.0	0.0
13	0.45986375670073965	0.0	0.0	0.0	0.0
14	0.49032000370199885	0.0	0.0	0.0	0.0
15	0.5194610669063224	0.0	0.0	0.0	1.2177627749403905E-5
16	0.5524015499684599	0.0	0.0	0.0	3.653288324821171E-5
17	0.5948405826751325	0.0	0.0	0.0	3.653288324821171E-5
18	0.6242617313176924	0.0	0.0	0.0	3.653288324821171E-5
19	0.6551441952901806	0.0	0.0	0.0	3.653288324821171E-5
20	0.6932114596348173	0.0	0.0	0.0	3.653288324821171E-5
21	0.7224986543721337	0.0	0.0	0.0	3.653288324821171E-5
22	0.7508603494004954	0.0	0.0	0.0	3.653288324821171E-5
23	0.7768839399009715	0.0	0.0	0.0	3.653288324821171E-5
24	0.8057570952948082	0.0	0.0	0.0	3.653288324821171E-5
25	0.8346424283163942	0.0	0.0	0.0	3.653288324821171E-5
26	0.8636495376154744	0.0	0.0	0.0	3.653288324821171E-5
27	0.8894661084442106	0.0	0.0	0.0	3.653288324821171E-5
28	0.9183027309547991	0.0	0.0	0.0	3.653288324821171E-5
29	0.9487833332115571	0.0	0.0	0.0	3.653288324821171E-5
30	0.9777051991163913	0.0	0.0	0.0	3.653288324821171E-5
31	1.003740967244617	0.0	0.0	0.0	3.653288324821171E-5
32	1.0317738663237446	0.0	0.0	0.0	3.653288324821171E-5
33	1.060817508506073	0.0	0.0	0.0	3.653288324821171E-5
34	1.0899829269658954	0.0	0.0	0.0	3.653288324821171E-5
35	1.1180401813005219	0.0	0.0	0.0	3.653288324821171E-5
36	1.1405809702646685	0.0	0.0	0.0	3.653288324821171E-5
37	1.1571790768871062	0.0	0.0	0.0	3.653288324821171E-5
38	1.1717313420476438	0.0	0.0	0.0	3.653288324821171E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAACG	85	0.0	133.03502	44
GTACCGA	80	0.0	131.2533	44
CAACGAA	30	9.354488E-6	107.69501	44
GGCCGAC	165	0.0	102.799774	44
AATACGG	145	0.0	94.69734	44
CGCATTC	95	0.0	90.700584	43
CCGATAA	85	0.0	89.44521	43
TATTAGG	295	0.0	87.625984	43
GATATGC	290	0.0	86.34169	44
CTATCAT	9120	0.0	82.80825	44
TACGATC	135	0.0	77.77973	44
AACCGCT	95	1.8189894E-12	76.520134	44
GCGCCAA	185	0.0	73.9736	43
GTTCGGC	760	0.0	69.35927	43
ACACGTC	140	0.0	69.052315	42
ATACCGG	40	1.5279511E-10	69.05231	42
CGTAGCG	140	0.0	68.78763	43
GAACACA	5705	0.0	68.66618	44
AACGACA	3240	0.0	67.73726	43
CGAATTA	235	0.0	67.58311	42
>>END_MODULE