FastQCFastQC Report
Tue 24 May 2016
ERR841128.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841128.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2302577
Sequences flagged as poor quality0
Sequence length24-50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC260321.1305593689157842No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA145010.6297726416966729No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT128520.5581572299210841No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA93640.4066747822114092No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT79360.34465731222017765No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC69110.300141971365127No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT65470.2843335966614797No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG64990.2822489758214383No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA59970.26044731620267203No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC52290.22709338276200972No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA41390.17975511785273632No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC40830.17732306020602134No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA40760.17701905300018195No Hit
AGGAAAGGCACTTGCTGCTGAGGCAGG38750.16828970323250864No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGA37260.16181869270821345No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT36130.15691114781394933No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG35440.1539145053563898No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT35330.15343677974721368No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA34530.149962411680478No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG33040.14349140115618284No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA32750.14223194273199113No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA32230.13997360348861299No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT30380.13193912733428675No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA28220.12255833355410047No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG28220.12255833355410047No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA27630.11999598710488292No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA26110.11339468777808517No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG24940.10831342448048425No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC24870.10800941727464489No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA24430.10609851483794026No Hit
CACACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTT24270.10540364122459314No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA23280.10110411074200777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCC1150.0202.7050644
TAGACCG800.0202.7050544
CGTGAAC153.2345073E-5202.7050544
CCGGATG900.0202.7050544
TCGGGGT255.009497E-9202.7050544
CGCCTAA255.009497E-9202.7050544
GTATGGG306.366463E-11202.7050544
ACAATTG550.0202.7050544
CGGTCCA153.2345073E-5202.7050544
ACGGTGC500.0202.7050544
CCCTCGG950.0192.0363644
CAAAGCG900.0191.4436644
ACCCGAA1250.0186.4886544
AATCGTA600.0185.8129644
CCTATGA800.0177.3669144
TCATGCT1200.0160.4748444
ACAACTG850.0155.0097544
GTGCCCC550.0147.4218444
GGCAAAT1250.0145.9476344
ATAATAA2200.0142.8149344
Produced by FastQC (version 0.11.5)