FastQCFastQC Report
Tue 24 May 2016
ERR841127.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841127.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7854646
Sequences flagged as poor quality0
Sequence length24-50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA634950.8083750687172917No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA609750.7762921460750746No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA594230.7565331397493916No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA494320.6293345365278079No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA480780.6120963312668706No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA419830.5344989449556352No Hit
AAAAAAAAAAAAAAAAAAAAAAAA395830.5039437805344761No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA317990.40484319726184986No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT217710.27717353525543986No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA185020.2355548550501194No Hit
AGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA181950.23164634026791278No Hit
AGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAA181850.2315190270828246No Hit
AGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA152640.19433084571857218No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA122450.1558949951404557No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA116250.14800157766498961No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA112410.14311275135760415No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98470.12536529335631422No Hit
AGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA97920.12466507083832931No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT95890.1220806131810396No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA95260.12127854011498418No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT94270.12001813958261137No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA93620.11919060387953831No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA92130.11729363742172466No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA90540.11526935777882287No Hit
AGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAA88600.11279948198811253No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG81260.10345469420264133No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT80020.10187601070754812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGC308.003553E-9187.4578644
ATAGCGA206.727241E-5168.7120744
CGTCGCT1000.0168.7120744
CGGGCAA1900.0165.752244
AAACACG1350.0158.2977444
TATCGGG2450.0151.4965544
TTAACCG1200.0149.966344
CAAACGA1800.0124.9719144
ACATTGG8700.0124.1100244
CCTCGGT1500.0119.9730344
TGCACGA751.8189894E-12119.9730344
TTAACGG500.0114.7558343
ACGGGCA1450.0110.7987443
GGCTTAC2550.0110.26932544
TCGGTAC1150.0109.7664543
TGTACAC2000.0106.85097544
CGAATGG7450.0106.2839243
ACTTCTA25200.0106.2261244
GGTCTTA4450.0106.1559144
CGGACAT500.0103.2802443
Produced by FastQC (version 0.11.5)