##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841127.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7854646
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.35492166037782	39.0	37.0	39.0	34.0	39.0
2	37.89313471288203	39.0	38.0	39.0	35.0	39.0
3	37.77112259419457	39.0	37.0	39.0	35.0	39.0
4	38.36838337462949	39.0	38.0	40.0	35.0	41.0
5	39.06214678548212	40.0	38.0	41.0	35.0	41.0
6	38.95202533634234	40.0	38.0	41.0	35.0	41.0
7	39.05194059668634	40.0	38.0	41.0	35.0	41.0
8	38.8500809584544	40.0	38.0	41.0	35.0	41.0
9	38.939764312739236	40.0	38.0	41.0	35.0	41.0
10	38.9506980963878	40.0	38.0	41.0	35.0	41.0
11	38.88758780472092	40.0	38.0	41.0	35.0	41.0
12	38.8498964816492	40.0	38.0	41.0	35.0	41.0
13	38.68720678182059	40.0	38.0	41.0	35.0	41.0
14	38.68167285960437	40.0	38.0	41.0	35.0	41.0
15	38.552529038227824	40.0	38.0	41.0	35.0	41.0
16	38.49199174603159	40.0	37.0	41.0	35.0	41.0
17	38.400056985381646	40.0	37.0	41.0	35.0	41.0
18	38.480720837068915	40.0	37.0	41.0	35.0	41.0
19	38.47804076720962	40.0	37.0	41.0	35.0	41.0
20	38.56658161297148	40.0	38.0	41.0	35.0	41.0
21	38.51248522212204	40.0	37.0	41.0	35.0	41.0
22	38.47362669176943	40.0	37.0	41.0	35.0	41.0
23	38.453567990206054	40.0	37.0	41.0	35.0	41.0
24	38.33619809218646	40.0	37.0	41.0	34.0	41.0
25	38.345748546161175	40.0	37.0	41.0	34.0	41.0
26	38.29939761772708	40.0	37.0	41.0	34.0	41.0
27	38.27704169648684	40.0	37.0	41.0	34.0	41.0
28	38.24277576717674	40.0	37.0	41.0	34.0	41.0
29	38.2249674834937	40.0	37.0	41.0	34.0	41.0
30	38.21549884494738	40.0	37.0	41.0	34.0	41.0
31	38.113729060132854	40.0	37.0	41.0	34.0	41.0
32	37.86248611709593	40.0	36.0	41.0	33.0	41.0
33	37.77741383647799	39.0	35.0	41.0	33.0	41.0
34	37.76518064138886	39.0	35.0	41.0	33.0	41.0
35	37.74190969656101	39.0	35.0	41.0	33.0	41.0
36	37.7002352393447	39.0	35.0	41.0	33.0	41.0
37	37.64415161222616	39.0	35.0	41.0	33.0	41.0
38	37.60439711233232	39.0	35.0	41.0	33.0	41.0
39	37.490385841834296	39.0	35.0	41.0	33.0	41.0
40	37.42154693315972	39.0	35.0	41.0	33.0	41.0
41	37.329284027743086	39.0	35.0	41.0	33.0	41.0
42	37.21012451863913	38.0	35.0	41.0	33.0	41.0
43	37.1161807655838	38.0	35.0	41.0	33.0	41.0
44	36.98352710111711	38.0	35.0	40.0	33.0	41.0
45	36.71990196666267	37.0	35.0	40.0	32.0	41.0
46	36.45105096921525	37.0	35.0	40.0	31.0	41.0
47	36.21720496586667	37.0	35.0	40.0	31.0	41.0
48	36.209056807223774	37.0	35.0	40.0	31.0	41.0
49	36.144833668524576	37.0	34.0	40.0	31.0	41.0
50	35.9687181503863	37.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	9.0
20	32.0
21	83.0
22	197.0
23	398.0
24	834.0
25	1625.0
26	3304.0
27	6650.0
28	12955.0
29	25295.0
30	47091.0
31	83821.0
32	140483.0
33	235174.0
34	363330.0
35	408963.0
36	752932.0
37	1239747.0
38	1309389.0
39	1546958.0
40	1675375.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	49.510149789054786	20.860570928339737	29.62927928260548
2	28.45077932219988	25.64906171455722	27.51059691296081	18.389562050282088
3	20.584250391424387	25.676001184521873	31.54518994236023	22.19455848169351
4	23.567809421328473	29.259358092013315	29.924213007180718	17.248619479477497
5	18.86096458070803	27.093608037841555	29.577259115178457	24.46816826627196
6	19.182939625795996	32.27009848693372	28.32957716999595	20.217384717274335
7	28.791125659896068	25.9704893129493	21.85552346980373	23.3828615573509
8	20.852728436138307	26.80912672576205	31.168508421639878	21.16963641645976
9	26.35568808575205	29.65293407239486	25.831870717025314	18.15950712482778
10	22.56077485859961	24.425925751459708	27.03792125068399	25.975378139256687
11	23.729522629027457	27.225670004733505	28.69668473919767	20.34812262704137
12	23.38435112161643	27.206051042911415	30.488719669861634	18.920878165610517
13	20.83463723253728	30.19609031393649	26.09852818319247	22.870744270333763
14	22.717051793295333	25.879142102648544	29.753549682570036	21.650256421486088
15	20.156210731839476	31.42802361812359	31.094577655059187	17.32118799497775
16	27.912499175647127	31.406825972806413	21.44887242531363	19.23180242623283
17	22.71666985374007	30.6069426935345	27.835856638224055	18.84053081450138
18	27.14786891732613	28.44645067390688	21.957323092600227	22.448357316166764
19	26.763281247811804	28.123558973886283	21.802395677666443	23.31076410063547
20	25.51253105486867	28.61307817055027	24.860623890624733	21.013766883956322
21	24.194012053503112	33.473144429424316	23.060517304026177	19.27232621304639
22	24.325411991832606	34.65529827824195	20.885537043935525	20.133752685989922
23	24.302559275109278	33.153741110675135	23.61742336955733	18.926276244658258
24	24.192662533741178	34.73788634140864	22.41138556721716	18.658065557633023
25	24.040462517686343	33.45299757049493	22.70344388419389	19.803096027624832
26	22.712801537695977	37.55278605868824	21.68980990055141	18.044602503064368
27	22.905389625366244	33.18962882518363	24.051012261948564	19.853969287501563
28	23.53490830240448	32.958069379006474	22.485867346342765	21.021154972246276
29	22.258947693832475	32.30560172244208	24.722822152579734	20.712628431145706
30	25.878317790032657	32.45508962074939	24.259841281658385	17.406751307559567
31	23.72983086055842	34.05546472763245	23.524501094969747	18.690203316839384
32	22.504135017457035	31.425712911459026	25.987739765515162	20.08241230556878
33	22.48044395116537	30.994509803921567	28.485445800961894	18.039600443951166
34	22.225174487306237	33.97646376165768	24.675845761889427	19.122515989146653
35	21.292120879506044	31.84686442980605	24.77410060390623	22.086914086781675
36	21.089223062700928	32.56850557291238	24.907499577267835	21.43477178711886
37	20.231232692832474	36.175148913951105	23.74859127265965	19.845027120556775
38	24.261912634360904	33.35321355146542	22.048202624706615	20.336671189467058
39	20.910194919927058	34.716179638640874	23.306112805407906	21.067512636024162
40	19.786721153086418	34.04284583112506	23.166601130790358	23.003831884998167
41	19.7482350261546	36.57363116197047	24.22815461825643	19.449979193618507
42	20.52469998257638	36.035670055741505	22.904707493676696	20.53492246800542
43	19.605250997902285	33.43203693599373	24.707718756765036	22.254993309338946
44	21.293561606138965	32.51780633588604	23.452699814342132	22.73593224363286
45	21.578693155321048	32.68978761109113	26.229080891660338	19.50243834192749
46	20.26215253640658	32.01521858739798	25.603744338972422	22.118884537223014
47	20.453459540853366	33.79910772949253	24.840535189968314	20.906897539685787
48	23.995176870182107	30.712994088511696	24.40613424925795	20.885694792048252
49	20.412484034079313	31.34591622705556	22.63038851006577	25.61121122879936
50	19.590927371544073	32.6883434223741	23.781193572055674	23.93953563402615
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	503948.0
1	500195.5
2	335741.5
3	173169.0
4	164263.0
5	114906.5
6	48648.5
7	25529.0
8	25898.0
9	23790.0
10	20727.0
11	18162.0
12	16314.5
13	16167.5
14	19175.5
15	23466.0
16	28333.5
17	39090.5
18	59818.0
19	93230.5
20	134725.0
21	171500.0
22	184112.0
23	174727.0
24	160316.5
25	151857.5
26	141718.5
27	134635.5
28	139176.0
29	143175.5
30	152441.0
31	174625.5
32	205291.0
33	221323.0
34	235227.5
35	257400.5
36	267289.5
37	273453.0
38	286411.5
39	316200.0
40	347119.0
41	353347.5
42	360223.5
43	384165.5
44	398201.0
45	399269.5
46	438077.0
47	499210.0
48	566750.0
49	610143.0
50	647908.5
51	696757.0
52	676631.0
53	616276.0
54	570734.0
55	528056.0
56	484940.0
57	455727.0
58	432604.5
59	391731.0
60	326505.0
61	266361.5
62	221510.0
63	182718.0
64	149107.0
65	124095.5
66	91017.5
67	59928.0
68	43421.5
69	34518.0
70	30174.5
71	23547.0
72	16432.5
73	9978.0
74	5552.5
75	3546.0
76	2260.5
77	1439.0
78	1364.5
79	1336.0
80	1090.0
81	839.5
82	389.5
83	46.0
84	44.5
85	46.5
86	49.0
87	38.5
88	30.5
89	31.0
90	21.0
91	7.0
92	3.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	78165.0
25	93818.0
26	108092.0
27	127102.0
28	130416.0
29	117381.0
30	115458.0
31	175537.0
32	151177.0
33	43258.0
34	38040.0
35	34881.0
36	37843.0
37	37487.0
38	35258.0
39	38106.0
40	47489.0
41	57259.0
42	68623.0
43	94365.0
44	220811.0
45	595168.0
46	766822.0
47	869436.0
48	1131873.0
49	1295592.0
50	1345189.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.593600961738527
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.02048139238545	7.417690390197691
2	12.102415947631235	2.564163304443719
3	4.83290795613964	1.53593695116464
4	2.562003950738396	1.085633900660807
5	1.59397145149638	0.8442948750777903
6	1.0912813956052492	0.6936359785206678
7	0.8015136204889722	0.5943640822600954
8	0.6323743469106172	0.535929719168887
9	0.4927930304390203	0.46984074490771643
>10	4.033032718801007	8.673611392990736
>50	0.5335345381544649	4.006766760364839
>100	1.0252672894015675	27.665057414851525
>500	0.20897851943601312	14.260775988228161
>1k	0.05909196524816057	12.370791426261066
>5k	0.006901251415843571	5.3602452456229415
>10k+	0.0034506257079217856	11.92126182527872
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA	63495	0.8083750687172917	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	60975	0.7762921460750746	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	59423	0.7565331397493916	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	49432	0.6293345365278079	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	48078	0.6120963312668706	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	41983	0.5344989449556352	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	39583	0.5039437805344761	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31799	0.40484319726184986	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	21771	0.27717353525543986	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18502	0.2355548550501194	No Hit
AGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	18195	0.23164634026791278	No Hit
AGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAA	18185	0.2315190270828246	No Hit
AGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	15264	0.19433084571857218	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA	12245	0.1558949951404557	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11625	0.14800157766498961	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA	11241	0.14311275135760415	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9847	0.12536529335631422	No Hit
AGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9792	0.12466507083832931	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9589	0.1220806131810396	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9526	0.12127854011498418	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9427	0.12001813958261137	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9362	0.11919060387953831	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA	9213	0.11729363742172466	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA	9054	0.11526935777882287	No Hit
AGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAA	8860	0.11279948198811253	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG	8126	0.10345469420264133	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT	8002	0.10187601070754812	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.23169726554194803	0.0	0.0	0.0	0.0
2	0.339824353637325	0.0	0.0	0.0	0.0
3	0.4222087157078753	0.0	0.0	0.0	0.0
4	0.5454988041472525	0.0	0.0	0.0	0.0
5	0.61629766637478	0.0	0.0	0.0	0.0
6	0.802455005610692	0.0	0.0	0.0	0.0
7	0.8883277489526581	0.0	0.0	0.0	0.0
8	1.005048477041486	0.0	0.0	0.0	0.0
9	1.1177970337555632	0.0	0.0	0.0	0.0
10	1.1950634057855694	0.0	0.0	0.0	0.0
11	1.3077737685441202	0.0	0.0	0.0	0.0
12	1.3885539844825598	0.0	0.0	0.0	0.0
13	1.5091450333980678	0.0	0.0	0.0	0.0
14	1.584819990614472	0.0	0.0	0.0	0.0
15	1.699872915978645	0.0	0.0	0.0	0.0
16	1.7887120565331651	0.0	0.0	0.0	1.273131850881631E-5
17	1.8993599456932877	0.0	0.0	0.0	5.092527403526524E-5
18	1.9832593346663872	0.0	0.0	0.0	5.092527403526524E-5
19	2.068317273623789	0.0	0.0	0.0	5.092527403526524E-5
20	2.1617014948859565	0.0	0.0	0.0	5.092527403526524E-5
21	2.248503624479066	0.0	0.0	0.0	5.092527403526524E-5
22	2.3310407623717224	0.0	0.0	1.273131850881631E-5	5.092527403526524E-5
23	2.403851172923643	0.0	0.0	1.273131850881631E-5	5.092527403526524E-5
24	2.4723838604566013	0.0	0.0	1.273131850881631E-5	5.092527403526524E-5
25	2.5393887897685015	0.0	0.0	1.273131850881631E-5	5.092527403526524E-5
26	2.602230577928019	0.0	0.0	1.273131850881631E-5	5.092527403526524E-5
27	2.660145345824624	1.273131850881631E-5	0.0	1.273131850881631E-5	5.092527403526524E-5
28	2.718225620861844	1.273131850881631E-5	0.0	1.273131850881631E-5	5.092527403526524E-5
29	2.7753637783294116	1.273131850881631E-5	0.0	1.273131850881631E-5	5.092527403526524E-5
30	2.8303885369245156	1.273131850881631E-5	0.0	1.273131850881631E-5	5.092527403526524E-5
31	2.8799515598793377	1.273131850881631E-5	0.0	1.273131850881631E-5	5.092527403526524E-5
32	2.9283687641683662	1.273131850881631E-5	0.0	1.273131850881631E-5	5.092527403526524E-5
33	2.97393415311142	1.273131850881631E-5	0.0	1.273131850881631E-5	5.092527403526524E-5
34	3.016762308575078	1.273131850881631E-5	0.0	1.273131850881631E-5	5.092527403526524E-5
35	3.0530720289622217	1.273131850881631E-5	0.0	1.273131850881631E-5	5.092527403526524E-5
36	3.083614462064872	1.273131850881631E-5	0.0	1.273131850881631E-5	5.092527403526524E-5
37	3.1029915288352905	1.273131850881631E-5	0.0	1.273131850881631E-5	5.092527403526524E-5
38	3.1197459439928927	1.273131850881631E-5	0.0	1.273131850881631E-5	5.092527403526524E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTCGC	30	8.003553E-9	187.45786	44
ATAGCGA	20	6.727241E-5	168.71207	44
CGTCGCT	100	0.0	168.71207	44
CGGGCAA	190	0.0	165.7522	44
AAACACG	135	0.0	158.29774	44
TATCGGG	245	0.0	151.49655	44
TTAACCG	120	0.0	149.9663	44
CAAACGA	180	0.0	124.97191	44
ACATTGG	870	0.0	124.11002	44
CCTCGGT	150	0.0	119.97303	44
TGCACGA	75	1.8189894E-12	119.97303	44
TTAACGG	50	0.0	114.75583	43
ACGGGCA	145	0.0	110.79874	43
GGCTTAC	255	0.0	110.269325	44
TCGGTAC	115	0.0	109.76645	43
TGTACAC	200	0.0	106.850975	44
CGAATGG	745	0.0	106.28392	43
ACTTCTA	2520	0.0	106.22612	44
GGTCTTA	445	0.0	106.15591	44
CGGACAT	50	0.0	103.28024	43
>>END_MODULE