##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841126.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2400952
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.02654322118893	35.0	35.0	39.0	27.0	39.0
2	35.894851292320716	37.0	35.0	39.0	33.0	39.0
3	36.21679108953448	37.0	35.0	39.0	33.0	39.0
4	36.310811711354496	37.0	35.0	39.0	33.0	39.0
5	37.17801188861751	37.0	35.0	41.0	34.0	41.0
6	37.07356748489766	36.0	35.0	41.0	34.0	41.0
7	37.092625341947695	37.0	35.0	41.0	34.0	41.0
8	37.06897722236846	37.0	35.0	41.0	34.0	41.0
9	37.07422805620437	37.0	35.0	41.0	34.0	41.0
10	37.05660379716046	37.0	35.0	41.0	34.0	41.0
11	36.97522191197492	36.0	35.0	41.0	33.0	41.0
12	36.794298261689526	36.0	35.0	40.0	33.0	41.0
13	36.706545986758584	36.0	35.0	40.0	33.0	41.0
14	36.713575698306336	36.0	35.0	40.0	33.0	41.0
15	36.698268020351925	36.0	35.0	40.0	33.0	41.0
16	36.65239371715886	35.0	35.0	40.0	33.0	41.0
17	36.77422330808779	36.0	35.0	40.0	33.0	41.0
18	36.81374887961109	36.0	35.0	40.0	33.0	41.0
19	36.79260601628021	36.0	35.0	41.0	33.0	41.0
20	36.82970296782276	36.0	35.0	41.0	33.0	41.0
21	36.844622466421654	36.0	35.0	41.0	33.0	41.0
22	36.821028492031495	36.0	35.0	41.0	33.0	41.0
23	36.774392407678285	35.0	35.0	41.0	33.0	41.0
24	36.70814243683339	35.0	35.0	40.0	33.0	41.0
25	36.76771518746743	36.0	35.0	40.0	33.0	41.0
26	36.769613208689194	36.0	35.0	41.0	33.0	41.0
27	36.73767310504558	35.0	35.0	41.0	33.0	41.0
28	36.74752371450987	35.0	35.0	41.0	33.0	41.0
29	36.707515221831095	35.0	35.0	41.0	33.0	41.0
30	36.63113103472429	35.0	35.0	40.0	33.0	41.0
31	36.60793230133793	35.0	35.0	40.0	33.0	41.0
32	36.638426078322084	35.0	35.0	40.0	33.0	41.0
33	36.39837888544074	35.0	35.0	40.0	33.0	41.0
34	36.46171804147158	35.0	35.0	40.0	33.0	41.0
35	36.443011487380964	35.0	35.0	40.0	33.0	41.0
36	36.500359512231896	35.0	35.0	40.0	33.0	41.0
37	36.499375173135526	35.0	35.0	40.0	33.0	41.0
38	36.42743800913547	35.0	35.0	40.0	33.0	41.0
39	36.39840736183312	35.0	35.0	40.0	33.0	41.0
40	36.41606136279478	35.0	35.0	40.0	33.0	41.0
41	36.34548414979218	35.0	35.0	40.0	33.0	41.0
42	36.28006804507503	35.0	35.0	40.0	33.0	41.0
43	36.22311640792736	35.0	35.0	39.0	33.0	41.0
44	36.11381657396584	35.0	35.0	39.0	33.0	41.0
45	35.964011557005676	35.0	35.0	39.0	33.0	41.0
46	35.79105419558315	35.0	35.0	39.0	32.0	41.0
47	35.532494516689944	35.0	34.0	39.0	31.0	41.0
48	35.49056933194365	35.0	34.0	39.0	31.0	41.0
49	35.55857773625616	35.0	34.0	39.0	31.0	41.0
50	35.59231382172825	36.0	34.0	38.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	11.0
21	27.0
22	83.0
23	226.0
24	641.0
25	1306.0
26	2803.0
27	5415.0
28	10084.0
29	18739.0
30	31841.0
31	54587.0
32	94574.0
33	190503.0
34	620205.0
35	187315.0
36	109133.0
37	157332.0
38	216347.0
39	324985.0
40	374793.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	50.79585097911162	19.829217743628362	29.374931277260018
2	24.544597309733806	25.04219159733306	30.646801768631775	19.76640932430136
3	23.225453903285032	24.82548589059673	29.19970911538423	22.74935109073401
4	20.851270662637152	26.084902988481236	33.697841522862596	19.365984826019012
5	20.54734955134463	25.67698146401927	32.08306538406432	21.692603600571772
6	20.01222848270186	29.078507192147114	29.278636140997406	21.63062818415362
7	24.164373131990978	25.969698686187815	24.75942875992523	25.106499421895982
8	26.781834872167376	27.03336010049347	25.812844238452083	20.371960788887076
9	24.10556312662644	26.639099823736583	27.01703324348009	22.238303806156893
10	26.949310106990893	23.572857766419318	27.263560454353108	22.21427167223668
11	26.81757069695687	25.547157960675598	26.753512773266603	20.88175856910092
12	24.184323551657844	27.533578347255588	25.550115121002005	22.731982980084567
13	25.086923853538096	27.100041983346607	24.063454829584266	23.74957933353103
14	20.341972684168613	28.409522556052764	27.21520463549459	24.033300124284036
15	22.830152372892087	30.42663909982374	27.178344256778143	19.564864270506032
16	20.973388889074002	32.91869225207334	24.98554739953152	21.122371459321133
17	22.830652174637393	31.23344406718668	24.801703657549172	21.134200100626753
18	21.76270079535118	28.799992669574404	24.854099540515595	24.583206994558825
19	23.48589226273578	26.97525814760145	25.581852531829043	23.956997057833725
20	21.941296619007794	27.47401863927309	27.366519613886492	23.218165127832627
21	24.277494927012285	28.562253639389706	26.8805040667202	20.279747366877803
22	24.57733432405146	28.10139478007057	26.03213225420583	21.289138641672135
23	24.664216527444115	28.455087815166653	26.388865749919198	20.491829907470034
24	23.4941806416788	29.39838030914404	25.856243690002966	21.251195359174194
25	24.162272887860247	29.48910205790809	23.778350467881847	22.570274586349818
26	24.735986211602132	30.674867919033794	24.080808747776782	20.508337121587292
27	22.84920537160018	30.498497351664888	24.807016990517823	21.845280286217104
28	25.131021646606328	28.31515485860661	24.35885537908414	22.19496811570292
29	25.936273320869212	25.696767847605095	26.050515696069287	22.3164431354564
30	24.430061113348625	28.85740802353477	26.770754898698932	19.941775964417673
31	25.228697921975474	29.2760373503747	24.462456846313046	21.03280788133678
32	23.684078265031264	26.146240039265635	27.7409083890902	22.4287733066129
33	23.487427692731064	26.996712587554317	28.7607682281256	20.75509149158902
34	23.836022793395856	29.348598669420106	25.645605845360954	21.169772691823088
35	21.093157173782362	26.839287884682257	28.314125128405355	23.75342981313003
36	23.60393343133929	26.57464472841042	27.01812167816352	22.803300162086774
37	22.16096809097517	27.843980329436945	27.419552653324086	22.5754989262638
38	22.90063331586944	27.48121680016788	27.773772662015656	21.84437722194702
39	23.82477637696543	28.364468421080275	26.70655663262602	21.104198569328272
40	21.188070623548818	27.428540770167842	27.86446494618732	23.51892366009603
41	21.35737505059702	28.15069210955685	29.149989279681105	21.341943560165024
42	21.121150741853516	28.497157708556998	28.271434970997817	22.110256578591674
43	23.647384300461933	26.609461439637368	27.24215649431298	22.500997765587723
44	22.358124593044693	28.86288842168023	26.492888578578654	22.286098406696425
45	23.368208946786087	26.92460285412006	30.307560914546	19.399627284547854
46	22.917835504612313	29.69210550658828	26.45382458372036	20.936234405079045
47	20.978416568150077	30.53456847179702	27.067571742632957	21.419443217419946
48	22.639347520968165	28.896806459487994	25.489370611083107	22.97447540846073
49	19.59727736812252	30.181175135964764	25.513440770522617	24.7081067253901
50	19.95615593821429	31.596155788575853	26.279876249013324	22.167812024196536
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	59697.0
1	59375.5
2	47020.5
3	34622.0
4	32043.5
5	20789.0
6	8003.0
7	5095.0
8	6596.5
9	7437.5
10	7410.0
11	7005.5
12	6221.0
13	5145.5
14	4867.0
15	5331.0
16	5625.0
17	6274.5
18	8049.0
19	9937.5
20	11574.0
21	14138.0
22	17272.5
23	23986.5
24	30364.5
25	29974.0
26	28571.0
27	32292.5
28	39515.0
29	43891.5
30	46183.5
31	58923.0
32	85388.0
33	102455.5
34	97960.5
35	95225.0
36	104267.5
37	112481.5
38	119726.5
39	128945.0
40	136028.0
41	144973.5
42	151271.5
43	149329.5
44	153637.0
45	161426.0
46	173612.5
47	188308.5
48	190143.5
49	187337.0
50	197123.0
51	203117.0
52	192012.0
53	182705.5
54	178402.5
55	166499.0
56	150466.5
57	143353.5
58	140844.5
59	122180.5
60	93165.5
61	73292.0
62	60826.0
63	52047.5
64	45088.0
65	37820.0
66	26551.5
67	17843.0
68	13175.0
69	8743.5
70	5679.5
71	3666.0
72	2560.5
73	1502.0
74	605.5
75	332.0
76	181.0
77	136.0
78	108.5
79	90.5
80	61.0
81	40.0
82	28.0
83	29.0
84	37.0
85	31.0
86	25.0
87	12.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	17658.0
25	20728.0
26	16375.0
27	18713.0
28	22729.0
29	20469.0
30	27810.0
31	47923.0
32	57311.0
33	9914.0
34	10375.0
35	8627.0
36	10535.0
37	10306.0
38	7218.0
39	6742.0
40	7357.0
41	8594.0
42	11966.0
43	20066.0
44	58400.0
45	154179.0
46	260385.0
47	243495.0
48	423947.0
49	364508.0
50	534622.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.156403826066057
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.1339664465421	4.947505819891563
2	11.717033074121538	1.6770364064357182
3	4.7267373503283885	1.0147932377609823
4	2.5347465045509447	0.7255867833310378
5	1.5949615026200779	0.5707094299889197
6	1.1001852450859781	0.47240219383888293
7	0.7657805528357848	0.38361644147686935
8	0.5976539125801141	0.34216421973213423
9	0.4483643328151569	0.2887808604146949
>10	4.05547419887705	6.1091782907250956
>50	0.8323620717595277	4.285901665261395
>100	1.7716444395180386	32.52573273898421
>500	0.5681694799015745	28.587135824513705
>1k	0.14794785144045378	15.342800165334664
>5k	0.004351407395307463	2.1138567751160005
>10k+	6.216296279010663E-4	0.6127991471941181
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	13775	0.5737307534677911	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	7985	0.33257641135682847	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	7484	0.31170968849023223	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	7208	0.30021424834815524	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	6873	0.2862614496249821	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	6578	0.2739746567195013	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	6337	0.2639369716679051	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5052	0.21041653477453945	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	4983	0.2075426747390202	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA	4894	0.2038358117946548	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	4157	0.17313965460367386	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA	3674	0.15302263435503916	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	3531	0.14706666355678913	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAAG	3238	0.13486317094219294	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3222	0.134196768615116	No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTTC	3222	0.134196768615116	No Hit
CTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTACC	3132	0.13044825552530828	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3041	0.12665809229005828	No Hit
AGCCTGTATTTTTTTTAACAACATCCCCATTCCCCACCTGGTCCTCCCCC	3018	0.1257001389448852	No Hit
CTCTTTTTCCGGCTGGAACCATGGAGGG	2979	0.1240757832726352	No Hit
ATAGGTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGGTTTTATG	2938	0.12236812730950057	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2914	0.12136852381888519	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2899	0.12074377163725056	No Hit
ATACTTCTTGCTTCTCATGATGCATGTTTCTGTATGTTAATGACTTGTTG	2872	0.11961921771030824	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA	2658	0.11070608658565435	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	2650	0.11037288542211589	No Hit
CTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTTGAC	2600	0.1082903781500005	No Hit
CTCTTTCCGCCATCTTTCCGCGCCGCCACAATGGTGCGCATGAATGTCC	2539	0.10574971927801971	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTT	2493	0.10383381258767356	No Hit
ACTTTTCATTTTGTTCTGTACTAAGAAAAATTCTTCTGCCTTGGGATCCT	2492	0.10379216244223124	No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC	2478	0.10320906040603894	No Hit
AAAGGTGGGGAAAAGATTGAGAAATCGGATGGTTGCCGTGTCTGTGTAGA	2475	0.10308410996971201	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	2472	0.10295915953338508	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	2435	0.10141810415201971	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.06401627354482722	0.0	0.0	0.0	0.0
2	0.09587863480819275	0.0	0.0	0.0	0.0
3	0.12982350334367368	0.0	0.0	0.0	0.0
4	0.19829634245082783	0.0	0.0	0.0	0.0
5	0.23694767742128955	0.0	0.0	0.0	0.0
6	0.366604580183194	0.0	0.0	0.0	0.0
7	0.4071301716985596	0.0	0.0	0.0	0.0
8	0.456027442447829	0.0	0.0	0.0	0.0
9	0.5124217393767139	0.0	0.0	0.0	0.0
10	0.5568624445636564	0.0	0.0	0.0	0.0
11	0.6046768115314258	0.0	0.0	0.0	0.0
12	0.6499088694817722	0.0	0.0	0.0	0.0
13	0.697223434704234	0.0	0.0	0.0	0.0
14	0.7439548978905034	0.0	0.0	0.0	0.0
15	0.7909362619494267	0.0	0.0	0.0	0.0
16	0.8388755793535231	0.0	0.0	0.0	0.0
17	0.8883559521389849	0.0	0.0	0.0	0.0
18	0.9250080801282158	0.0	0.0	0.0	0.0
19	0.9607855550631583	0.0	0.0	0.0	0.0
20	1.0016443477420622	0.0	0.0	0.0	0.0
21	1.0344230122051585	0.0	0.0	0.0	0.0
22	1.0664519740502933	0.0	0.0	0.0	0.0
23	1.1007300437493128	0.0	0.0	0.0	0.0
24	1.1364242183933706	0.0	0.0	0.0	0.0
25	1.1766166087451977	0.0	0.0	0.0	0.0
26	1.2087705210266595	0.0	0.0	0.0	0.0
27	1.2412159843262172	0.0	0.0	0.0	0.0
28	1.2757439548978906	0.0	0.0	0.0	0.0
29	1.3080228176156792	0.0	0.0	0.0	0.0
30	1.3404266307697947	0.0	0.0	0.0	0.0
31	1.372247341887718	0.0	0.0	0.0	0.0
32	1.4012358431155643	0.0	0.0	0.0	0.0
33	1.433806256851449	0.0	0.0	0.0	0.0
34	1.4651271662240644	0.0	0.0	0.0	0.0
35	1.4970311776328722	0.0	0.0	0.0	0.0
36	1.5279355855510648	0.0	0.0	0.0	0.0
37	1.5450954454732957	0.0	0.0	0.0	0.0
38	1.5627967572862764	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTAAAC	25	1.1024895E-8	177.65567	44
TAGTTAC	45	0.0	177.65567	44
TGTATGG	200	0.0	173.21428	44
TCATTAC	165	0.0	166.88866	44
GCATATA	145	0.0	165.40353	44
CGCCCCA	65	0.0	163.98985	44
CGACGGG	60	0.0	162.85103	44
TGACGGC	110	0.0	161.50514	44
TCATCGC	105	0.0	160.73607	44
GTACAAT	100	0.0	159.8901	44
CGTCAGT	90	0.0	157.91614	44
AGACGCG	130	0.0	157.15694	44
AGCGCCT	85	0.0	156.755	44
GTGCCCC	100	0.0	151.00732	44
CGACATA	30	3.276E-8	148.04639	44
GACCGAA	95	0.0	140.25447	44
GACGGCA	140	0.0	139.5866	44
AGCTCGG	20	1.7221535E-4	133.24176	44
CCTTGCG	40	1.1714292E-9	133.24176	44
TGAGTAA	120	0.0	133.24174	44
>>END_MODULE