FastQCFastQC Report
Tue 24 May 2016
ERR841125.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841125.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2166176
Sequences flagged as poor quality0
Sequence length24-50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC129890.5996281003944278No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA122100.5636661102329635No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG112420.5189790672595394No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA76900.3550034715554045No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG71170.3285513273159706No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT61530.284048941544916No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC60530.279432511485678No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT54540.2517800954308422No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC50330.23234492488145006No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA46090.21277126143028083No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA45960.21217112552257988No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA43780.20210730799344098No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA43510.2008608718774467No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT43270.19975292866322958No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG39330.18156419422983175No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGA37940.17514735644749088No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA37530.1732546201232033No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT37100.17126955519773093No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA35490.1638371028023577No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA34600.15972848004963586No Hit
TAAATCTCCTGCTTGCTGTTCAGGTCGGGGATGTTATCTCCACCCCAGC34300.15834355103186445No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG32190.1486028836068722No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC31790.14675631158317698No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC30650.14149358131564566No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA28220.1302756562716972No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCA28080.1296293560634039No Hit
AAAAAAAAAAAAAAAAAAAAAAAA27980.1291677130574801No Hit
TCTCCCAGACAGTGGCTCAGCCTTCAGGACTCTCTTCCAGCATGGCCG27840.12852141284918678No Hit
CTCATTTTTCTACTGCTCGTGGATTTACGCGCACGTTGGAACCGAAGAG26930.12432046149528017No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC25540.1179036237129393No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT25460.11753430930820025No Hit
ACTCTGCGTCCTCGCCTCACCAGGAAACTACTAAAGTTCCTGGGGAAGC25350.11702650200168407No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT24710.11407198676377174No Hit
CTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAAG24310.11222541474007652No Hit
CTCTTCCTTTCTCCGCCATCGTGGTGTGTTCTTGACTCCGCTGCTCGCC23370.10788597048439277No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC23300.10756282038024612No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT23250.10733199887728422No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23170.10696268447254517No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA22790.10520844105003473No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGA22420.10350036192811665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAA1000.0266.494744
CCTTCGG1050.0266.494744
GTACCCC400.0266.494744
ACTGCAT800.0266.494744
ACCGCAA1300.0266.494744
CGGATAC201.0256372E-7266.494744
CTCGCTA550.0266.494744
CTTAGAA550.0266.494744
ACGGTCC950.0266.494744
GCAACAT1150.0254.9079744
CCAACGC850.0250.8185644
CCCTGTT2150.0247.9020544
CCATTGC1450.0238.926344
TAGTGAC400.0233.1828644
ACATGAG352.7284841E-11228.4240344
TTGTTAA650.0225.4955144
CGGACGG550.0218.0411244
CCCTTAG500.0213.1957444
CAGGCAC4200.0212.5612344
ACCACTC450.0207.2736744
Produced by FastQC (version 0.11.5)