##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841125.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2166176
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.62200486017757	37.0	35.0	39.0	30.0	39.0
2	37.00104839126645	38.0	37.0	39.0	34.0	39.0
3	36.915806933508634	38.0	35.0	39.0	33.0	39.0
4	37.16287919356507	38.0	36.0	39.0	33.0	40.0
5	37.505566029722424	38.0	36.0	40.0	33.0	41.0
6	37.43495357717932	38.0	36.0	40.0	33.0	41.0
7	37.4052916291197	38.0	36.0	40.0	33.0	41.0
8	37.3328944647157	38.0	36.0	40.0	33.0	41.0
9	37.27544899398756	38.0	36.0	40.0	32.0	41.0
10	37.2385050891525	38.0	36.0	40.0	33.0	41.0
11	37.07503037610979	38.0	36.0	40.0	32.0	41.0
12	36.946904129673676	38.0	35.0	40.0	32.0	41.0
13	36.89255489858626	38.0	35.0	40.0	32.0	41.0
14	36.75133737978816	38.0	35.0	40.0	31.0	41.0
15	36.722014739337894	38.0	35.0	40.0	31.0	40.0
16	37.071346003279515	38.0	36.0	40.0	33.0	40.0
17	37.36722408520822	38.0	36.0	40.0	33.0	41.0
18	37.55182035070096	39.0	36.0	40.0	33.0	41.0
19	37.63314753741155	39.0	36.0	40.0	33.0	41.0
20	37.717840101635325	39.0	37.0	40.0	33.0	41.0
21	37.819754719838095	39.0	37.0	40.0	33.0	41.0
22	37.76491660880741	39.0	37.0	40.0	33.0	41.0
23	37.750286218663675	39.0	36.0	40.0	33.0	41.0
24	37.641428028008804	39.0	36.0	40.0	33.0	41.0
25	37.710942273796896	39.0	36.0	40.0	33.0	41.0
26	37.51670759167467	39.0	36.0	40.0	33.0	41.0
27	37.58016211696387	39.0	36.0	40.0	33.0	41.0
28	37.63234363273877	39.0	36.0	40.0	33.0	41.0
29	37.56698806057841	39.0	36.0	40.0	33.0	41.0
30	37.566013864818025	39.0	36.0	40.0	33.0	41.0
31	37.42444394069026	39.0	36.0	40.0	33.0	41.0
32	37.229743821924146	39.0	35.0	40.0	32.0	41.0
33	37.172624416400495	38.0	35.0	40.0	32.0	41.0
34	37.319987110902744	39.0	36.0	40.0	33.0	41.0
35	37.35978787283823	39.0	35.0	40.0	33.0	41.0
36	37.41423738673252	39.0	36.0	40.0	33.0	41.0
37	37.38232649340267	39.0	35.0	40.0	33.0	41.0
38	37.43128882351044	39.0	36.0	40.0	33.0	41.0
39	37.415653756540706	39.0	35.0	40.0	33.0	41.0
40	37.31402130104087	39.0	35.0	40.0	33.0	41.0
41	37.28203671386367	39.0	35.0	40.0	33.0	41.0
42	37.28155660102154	38.0	35.0	40.0	33.0	41.0
43	37.13108664640344	38.0	35.0	40.0	32.0	41.0
44	36.930701358923166	38.0	35.0	40.0	32.0	41.0
45	36.68994670138938	38.0	35.0	40.0	31.0	41.0
46	36.37997780588017	38.0	34.0	40.0	31.0	41.0
47	36.11525314017336	37.0	34.0	40.0	31.0	41.0
48	36.07985964586141	37.0	34.0	40.0	31.0	40.0
49	35.840298002400004	37.0	34.0	40.0	30.0	40.0
50	35.646327794049824	37.0	34.0	39.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	9.0
21	31.0
22	72.0
23	130.0
24	276.0
25	501.0
26	821.0
27	1784.0
28	3958.0
29	8506.0
30	17831.0
31	35102.0
32	63667.0
33	103982.0
34	146037.0
35	193579.0
36	280175.0
37	371734.0
38	427905.0
39	387477.0
40	122596.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	49.173335869292245	20.95609959670867	29.870564533999083
2	22.37495937541548	22.437973645724078	31.99624591907583	23.190821059784618
3	18.83152615484614	22.808580650879705	30.57646285435717	27.783430339916976
4	23.033354630464007	24.072882351203226	31.006067835669864	21.887695182662906
5	17.790844326592115	23.726049960852674	32.1030701106466	26.38003560190862
6	18.111640051408564	25.598197007076063	33.034665696600825	23.25549724491454
7	23.885455290798163	24.64388858523038	24.31229041407531	27.15836570989615
8	22.961707635944634	22.040683674826052	27.46600460904377	27.531604080185545
9	21.854318393334612	23.82955032278079	28.72508974340035	25.591041540484245
10	23.418872704710974	21.656735186799224	30.93949891421565	23.98489319427415
11	23.19968460549835	24.516336622693633	26.950026221322737	25.33395255048528
12	23.194745025334967	26.49895484023459	25.42383444373864	24.8824656906918
13	24.702978889988625	22.89633898630582	26.829722053978994	25.57096006972656
14	19.603254767848966	26.29578575332752	25.72607211971696	28.374887359106555
15	22.08800208293324	28.867183460623696	27.0096243333875	22.03519012305556
16	24.81691238385062	27.692578996351173	23.87151367201926	23.618994947778944
17	22.096634807144017	30.3503039457551	24.73663266512047	22.81642858198041
18	21.673585156515447	27.962593990516005	22.74247337243142	27.62134748053713
19	22.902248016781645	25.994240541858094	26.223584787201037	24.879926654159217
20	25.221957957248165	23.96901267487037	25.70465188424209	25.10437748363937
21	22.37560567562377	27.677206284253913	25.380301508280027	24.56688653184229
22	26.475734197036623	27.11007785147652	22.594839939136985	23.819348012349874
23	29.73027122449884	27.374368472367895	21.809400528858227	21.085959774275036
24	24.362194023015675	30.620226611318746	22.844404148139393	22.173175217526182
25	26.489076391894073	26.497248435703657	24.992338708928514	22.02133646347375
26	24.821726178514044	30.695345017913844	23.420632747047932	21.062296056524183
27	22.081946381761185	30.616460195469642	26.08464394182375	21.216949480945424
28	25.262380891014807	27.8144590917016	23.39416072227234	23.528999295011257
29	24.08108966627418	30.22209378142189	22.335674724203898	23.361141828100035
30	27.03649143077219	28.270315809743884	22.4920566146736	22.20113614481032
31	26.11393457208113	28.324082943939473	24.48454977782003	21.077432706159367
32	23.896802376202157	27.395680446582716	27.377570363146976	21.32994681406815
33	20.283054939256022	26.480971579546026	31.243761391553427	21.992212089644532
34	21.52845756906128	26.50491155035173	27.051120134836655	24.91551074575034
35	20.98784747787104	24.167914232889938	29.963217248318852	24.881021040920174
36	21.281669027984833	24.565879749037357	26.38591904096052	27.76653218201729
37	19.962831372431406	27.666427597447424	26.640515562165152	25.730225467956018
38	23.41478359745371	25.18083394278854	26.61913089433347	24.78525156542428
39	21.71751483343924	26.94887464599791	26.74950903865716	24.584101481905687
40	19.84162247572675	25.490210512755123	29.371669247575305	25.29649776394282
41	20.08020762104407	29.775983652218912	26.66224882315513	23.481559903581886
42	20.48159797177242	29.12824402378288	27.172362216835772	23.21779578760893
43	20.883837857844416	24.151483931750196	28.777257755866003	26.187420454539385
44	19.568298756354476	26.72428023183254	26.1528711439493	27.554549867863678
45	20.070443420714433	24.546566377530414	28.52235462923995	26.8606355725152
46	19.104591694218865	29.518115886825225	26.54870009497255	24.82859232398336
47	22.54147493317319	27.736113348362718	26.43357515445696	23.288836564007127
48	20.797601896175117	28.929497606543663	26.0319747176651	24.24092577961612
49	18.529883818746917	29.276208052642378	25.91199361811715	26.281914510493547
50	20.668928803765205	26.470363662463082	28.7313808911517	24.129326642620008
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	39873.0
1	38988.5
2	25723.5
3	13268.0
4	12573.5
5	8902.5
6	3886.5
7	2137.5
8	2421.0
9	2567.5
10	2655.5
11	2555.0
12	2342.0
13	2260.0
14	2730.0
15	3848.0
16	4417.5
17	3669.5
18	2909.0
19	3476.5
20	4444.5
21	5281.0
22	7478.5
23	11356.5
24	14600.5
25	16285.5
26	18032.0
27	19915.0
28	23120.0
29	26706.5
30	29106.0
31	39533.0
32	55941.0
33	61819.5
34	66849.5
35	79103.5
36	85054.5
37	86700.0
38	91009.5
39	99495.0
40	112964.0
41	127282.0
42	136539.5
43	139588.5
44	149851.0
45	168311.0
46	180771.0
47	193048.5
48	208503.5
49	213030.5
50	235370.5
51	257716.5
52	234828.5
53	197326.5
54	162886.0
55	142899.0
56	144267.0
57	143515.5
58	128810.0
59	106520.0
60	83724.5
61	68807.5
62	60113.0
63	47680.0
64	36166.0
65	30106.0
66	21905.0
67	13344.5
68	9248.0
69	7161.5
70	5378.5
71	3812.0
72	2594.5
73	1570.0
74	846.5
75	806.5
76	758.5
77	659.0
78	346.5
79	78.0
80	46.5
81	23.5
82	8.5
83	6.5
84	7.0
85	6.0
86	4.0
87	2.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	12492.0
25	13998.0
26	13320.0
27	15694.0
28	17187.0
29	16285.0
30	25935.0
31	41335.0
32	35331.0
33	10148.0
34	8643.0
35	9861.0
36	12058.0
37	11407.0
38	10417.0
39	10280.0
40	7950.0
41	8445.0
42	13033.0
43	24644.0
44	77498.0
45	195023.0
46	228053.0
47	271289.0
48	340017.0
49	408414.0
50	327419.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.491137060534897
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.32486769454852	5.193220856033108
2	11.42123985112549	1.7111614625204843
3	4.762726478065211	1.0703471048707545
4	2.6599427755528016	0.797039836193826
5	1.6935448926662717	0.6343288454565952
6	1.146537765994491	0.5153322930086532
7	0.7938639820222995	0.416286072772574
8	0.6235633445014974	0.37369587836690055
9	0.47353508275366707	0.3192584587091509
>10	4.064720806957791	6.293586115841371
>50	0.729865222178919	3.874568105561608
>100	1.6448034114103498	32.692891870411806
>500	0.5271972899488799	27.73594999125456
>1k	0.12772641388131084	14.762744474981169
>5k	0.003909992261672781	1.830642996113852
>10k+	0.0019549961308363904	1.7789456379035968
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	12989	0.5996281003944278	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	12210	0.5636661102329635	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	11242	0.5189790672595394	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	7690	0.3550034715554045	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	7117	0.3285513273159706	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAAT	6153	0.284048941544916	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	6053	0.279432511485678	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	5454	0.2517800954308422	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	5033	0.23234492488145006	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	4609	0.21277126143028083	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	4596	0.21217112552257988	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	4378	0.20210730799344098	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	4351	0.2008608718774467	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	4327	0.19975292866322958	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	3933	0.18156419422983175	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGA	3794	0.17514735644749088	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA	3753	0.1732546201232033	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT	3710	0.17126955519773093	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	3549	0.1638371028023577	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3460	0.15972848004963586	No Hit
TAAATCTCCTGCTTGCTGTTCAGGTCGGGGATGTTATCTCCACCCCAGC	3430	0.15834355103186445	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG	3219	0.1486028836068722	No Hit
ATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC	3179	0.14675631158317698	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	3065	0.14149358131564566	No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAA	2822	0.1302756562716972	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCA	2808	0.1296293560634039	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	2798	0.1291677130574801	No Hit
TCTCCCAGACAGTGGCTCAGCCTTCAGGACTCTCTTCCAGCATGGCCG	2784	0.12852141284918678	No Hit
CTCATTTTTCTACTGCTCGTGGATTTACGCGCACGTTGGAACCGAAGAG	2693	0.12432046149528017	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	2554	0.1179036237129393	No Hit
ATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTT	2546	0.11753430930820025	No Hit
ACTCTGCGTCCTCGCCTCACCAGGAAACTACTAAAGTTCCTGGGGAAGC	2535	0.11702650200168407	No Hit
AGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCT	2471	0.11407198676377174	No Hit
CTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCAAAG	2431	0.11222541474007652	No Hit
CTCTTCCTTTCTCCGCCATCGTGGTGTGTTCTTGACTCCGCTGCTCGCC	2337	0.10788597048439277	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGC	2330	0.10756282038024612	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	2325	0.10733199887728422	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2317	0.10696268447254517	No Hit
CAGAGTGTTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTCAA	2279	0.10520844105003473	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGA	2242	0.10350036192811665	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.05465853190137828	0.0	0.0	0.0	0.0
2	0.09495996631852628	0.0	0.0	0.0	0.0
3	0.12505909030475826	0.0	0.0	0.0	0.0
4	0.1718235268048395	0.0	0.0	0.0	0.0
5	0.20159950068692478	0.0	0.0	0.0	0.0
6	0.28238702672359034	0.0	0.0	0.0	0.0
7	0.31793353817972314	0.0	0.0	0.0	0.0
8	0.3630360598584787	0.0	0.0	0.0	0.0
9	0.41686363434919416	0.0	0.0	0.0	0.0
10	0.45222548860295747	0.0	0.0	0.0	0.0
11	0.49820513199296823	0.0	0.0	0.0	0.0
12	0.5343056150562097	0.0	0.0	0.0	0.0
13	0.5698059622117501	0.0	0.0	0.0	0.0
14	0.6048446663613668	0.0	0.0	0.0	0.0
15	0.645653908085031	0.0	0.0	0.0	0.0
16	0.6829084986630819	0.0	0.0	0.0	0.0
17	0.7250103408033327	0.0	0.0	0.0	0.0
18	0.7523396067540218	0.0	0.0	0.0	0.0
19	0.7821155806361071	0.0	0.0	0.0	0.0
20	0.8223246864520704	0.0	0.0	0.0	0.0
21	0.85431654676259	0.0	0.0	0.0	0.0
22	0.8876010074896962	0.0	0.0	0.0	0.0
23	0.9134991801220215	0.0	0.0	0.0	0.0
24	0.9449370688254325	0.0	0.0	0.0	0.0
25	0.9771135863383216	0.0	0.0	0.0	0.0
26	1.006935724520999	0.0	0.0	0.0	0.0
27	1.0336648545639875	0.0	0.0	0.0	0.0
28	1.0604401489075679	0.0	0.0	0.0	0.0
29	1.0906316014949846	0.0	0.0	0.0	0.0
30	1.119207303561668	0.0	0.0	0.0	0.0
31	1.1483369772354601	0.0	0.0	0.0	0.0
32	1.1754354216831873	0.0	0.0	0.0	0.0
33	1.2023030446279526	0.0	0.0	0.0	0.0
34	1.231248061099375	0.0	0.0	0.0	0.0
35	1.2608855420796832	0.0	0.0	0.0	0.0
36	1.2838753637746887	0.0	0.0	0.0	0.0
37	1.300171361883799	0.0	0.0	0.0	0.0
38	1.3149439380733605	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCACAA	100	0.0	266.4947	44
CCTTCGG	105	0.0	266.4947	44
GTACCCC	40	0.0	266.4947	44
ACTGCAT	80	0.0	266.4947	44
ACCGCAA	130	0.0	266.4947	44
CGGATAC	20	1.0256372E-7	266.4947	44
CTCGCTA	55	0.0	266.4947	44
CTTAGAA	55	0.0	266.4947	44
ACGGTCC	95	0.0	266.4947	44
GCAACAT	115	0.0	254.90797	44
CCAACGC	85	0.0	250.81856	44
CCCTGTT	215	0.0	247.90205	44
CCATTGC	145	0.0	238.9263	44
TAGTGAC	40	0.0	233.18286	44
ACATGAG	35	2.7284841E-11	228.42403	44
TTGTTAA	65	0.0	225.49551	44
CGGACGG	55	0.0	218.04112	44
CCCTTAG	50	0.0	213.19574	44
CAGGCAC	420	0.0	212.56123	44
ACCACTC	45	0.0	207.27367	44
>>END_MODULE