FastQCFastQC Report
Tue 24 May 2016
ERR841124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6716265
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA800571.1919869153465505No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT415190.618185851809004No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC367740.5475364655801996No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG302590.4505331460268468No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC206850.30798367842841223No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA202950.3021768795602913No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC168580.25100260338149255No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT162270.24160750059743027No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG132190.19682070317356445No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA129880.19338129153629288No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA128180.19085012279890684No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA121530.18094878626736735No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA120590.17954919884787154No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT107180.15958274427825583No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA104350.15536909279190145No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT98120.146093103830775No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA92340.13748713012366248No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTA89130.132707687978363No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCC86330.1285387041756095No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT83150.12380392971391094No Hit
AAAAAAAAAAAAAAAAAAAAAAAA79670.11862247841620305No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC77820.11586797126081237No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCAT76830.1143939377019817No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68790.10242299849693244No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT68220.10157431250851477No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA67610.1006660696086292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCGG400.0195.4721844
CACGCTC1100.0177.7019844
CCGACGC1950.0170.4116544
GAGTACC1100.0168.8168844
ATATCGA950.0154.3201444
CGTAACA1350.0152.0339244
ATATCGT1150.0144.4794344
CTGGCGT1300.0135.3268944
CTATCAT112150.0118.869444
GATGCGT555.52609E-9106.62118544
AACGTCG209.839001E-6106.6070443
GTACCTG2800.0104.7172344
GTAACCC750.099.499943
GTACGGT650.098.40649443
TCACGCT1100.096.9154943
CCGGACT1200.088.83919543
AATATCG2000.087.950843
TCGTGCG1650.087.2239443
CGCGTGG1100.087.2239443
ACCTCGA800.086.6182243
Produced by FastQC (version 0.11.5)