##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841124.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6716265
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.595959956910576	39.0	35.0	39.0	32.0	39.0
2	37.252481997062354	39.0	37.0	39.0	33.0	39.0
3	37.39605152566195	39.0	37.0	39.0	34.0	39.0
4	37.969320894872375	39.0	37.0	40.0	35.0	41.0
5	38.67446891985352	40.0	37.0	41.0	35.0	41.0
6	38.68647380649811	40.0	37.0	41.0	35.0	41.0
7	38.68967454381267	40.0	37.0	41.0	35.0	41.0
8	38.63081593713172	40.0	37.0	41.0	35.0	41.0
9	38.54863618395046	40.0	37.0	41.0	35.0	41.0
10	38.54737327964278	40.0	37.0	41.0	35.0	41.0
11	38.454294164986045	40.0	37.0	41.0	34.0	41.0
12	38.414288745307104	40.0	37.0	41.0	34.0	41.0
13	38.32533379787724	40.0	36.0	41.0	34.0	41.0
14	38.28560204220649	40.0	36.0	41.0	34.0	41.0
15	38.207540351668676	40.0	36.0	41.0	34.0	41.0
16	38.2270173973183	40.0	36.0	41.0	34.0	41.0
17	38.36965500914571	40.0	36.0	41.0	35.0	41.0
18	38.38508203592324	40.0	36.0	41.0	35.0	41.0
19	38.343930443483096	40.0	36.0	41.0	35.0	41.0
20	38.33676798041769	40.0	35.0	41.0	34.0	41.0
21	38.39355564439462	40.0	36.0	41.0	35.0	41.0
22	38.399371525691734	40.0	35.0	41.0	35.0	41.0
23	38.42074322558744	40.0	36.0	41.0	35.0	41.0
24	38.30152071128819	40.0	35.0	41.0	34.0	41.0
25	38.33216398979297	40.0	35.0	41.0	34.0	41.0
26	38.26096636462709	40.0	35.0	41.0	34.0	41.0
27	38.23492469938031	40.0	35.0	41.0	34.0	41.0
28	38.190149605448354	40.0	35.0	41.0	34.0	41.0
29	38.17895570774443	40.0	35.0	41.0	34.0	41.0
30	38.19579439527411	40.0	35.0	41.0	34.0	41.0
31	38.145188561934	40.0	35.0	41.0	34.0	41.0
32	37.929555831076144	40.0	35.0	41.0	34.0	41.0
33	37.82402027832094	40.0	35.0	41.0	34.0	41.0
34	37.892057371947104	40.0	35.0	41.0	34.0	41.0
35	37.87503496149136	39.0	35.0	41.0	34.0	41.0
36	37.93103765422314	39.0	35.0	41.0	34.0	41.0
37	37.84991595479204	39.0	35.0	41.0	34.0	41.0
38	37.76975437022934	39.0	35.0	41.0	34.0	41.0
39	37.7631433908982	39.0	35.0	41.0	34.0	41.0
40	37.70490188215927	39.0	35.0	41.0	34.0	41.0
41	37.59157227680897	39.0	35.0	41.0	34.0	41.0
42	37.49380919779709	39.0	35.0	41.0	34.0	41.0
43	37.401609845859994	39.0	35.0	41.0	34.0	41.0
44	37.27104548091518	38.0	35.0	40.0	34.0	41.0
45	37.02425586740419	37.0	35.0	40.0	33.0	41.0
46	36.79641759809271	37.0	35.0	40.0	33.0	41.0
47	36.56987397413815	37.0	35.0	40.0	32.0	41.0
48	36.571030526498085	37.0	35.0	40.0	32.0	41.0
49	36.7370579090169	37.0	35.0	40.0	33.0	41.0
50	36.70959401393882	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	12.0
21	53.0
22	121.0
23	311.0
24	786.0
25	1821.0
26	3595.0
27	7121.0
28	14338.0
29	27178.0
30	49057.0
31	83672.0
32	141570.0
33	275931.0
34	734435.0
35	322220.0
36	381662.0
37	608799.0
38	851668.0
39	1309244.0
40	1902670.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	46.57234340812937	18.555238663155787	34.872417928714846
2	22.625268657505323	21.284449020400476	33.32617161472932	22.764110707364882
3	21.557785465582434	24.451715350719486	26.86235578852234	27.12814339517574
4	19.749548298049586	26.70581044672895	29.724914070543672	23.819727184677795
5	15.180297382548188	29.547568477420118	31.532094102897968	23.740040037133735
6	18.846159286448643	28.04125507257382	28.347050034505784	24.765535606471754
7	21.242357173220533	26.14028779388544	23.739102015778116	28.878253017115913
8	21.272894979575703	24.386113412737586	30.031259933906718	24.309731673779993
9	19.444066009902823	29.96938030289156	28.57012044640883	22.016433240796783
10	25.31898309551514	22.648227846876203	27.983827320690892	24.048961736917764
11	22.669727891916118	25.0733703926215	24.44031020217338	27.81659151328901
12	18.897765350235584	27.48587198390772	26.207438211565503	27.40892445429119
13	22.313249998324963	26.235400181499685	24.639587627944994	26.811762192230354
14	17.69304814506277	26.94505056009553	23.7880726862326	31.5738286086091
15	20.7798233095329	34.10901743751922	26.079703525694715	19.031455727253167
16	18.85883001936344	35.00506605978174	23.454137083632048	22.681966837222774
17	20.1937386330051	37.38496024203929	22.694518456314633	19.726782668640976
18	19.911230423457084	27.853457241487646	23.425996442963463	28.809315892091806
19	22.487260404406317	26.623294345890162	23.338566896928576	27.550878352774944
20	21.162655136448606	23.022587703135596	29.041692071411713	26.773065089004085
21	17.833126000835286	34.17333890190456	24.75959480455283	23.23394029270733
22	23.430850331248095	26.680930546963232	22.940696949867228	26.947522171921445
23	25.14869499640053	25.203040678114995	28.32744092140498	21.3208234040795
24	19.11230423457085	28.76213192898136	26.2024354310022	25.923128405445585
25	22.552207652876984	26.302962466829392	24.080655335841637	27.064174544451987
26	21.02280627041477	33.03759389642835	26.254527536737715	19.68507229641917
27	23.3397166452979	28.105983618897763	23.72191099908697	24.83238873671737
28	22.11709981578063	24.956856780099724	23.8194592084696	29.106584195650047
29	19.89438088194241	29.10392107436274	26.7625013350126	24.239196708682247
30	25.42127151204166	27.72455911681154	27.40459136299292	19.44957800815387
31	24.807511307638727	27.93602720129892	23.504147060938056	23.7523144301243
32	18.571265728328537	31.02384565794839	25.77135743134487	24.633531182378203
33	20.012241420396492	22.502440123132367	33.65182788889361	23.833490567577538
34	21.37894323149544	30.32921543619783	26.11649161588539	22.175349716421337
35	18.55502593237773	26.636049723302417	26.2540399031542	28.55488444116565
36	19.678013790917017	26.27891271036432	26.975013870175164	27.0680596285435
37	17.853470795119406	34.87778287812715	22.33765079381926	24.93109553293418
38	25.201696159966524	25.139111291533954	23.2836383248128	26.37555422368672
39	19.77536792665479	30.241604556852636	26.086272387792654	23.89675512869992
40	17.273897443107558	26.462724414717293	28.348641893118483	27.91473624905667
41	19.317776587229563	31.837210453492236	23.505406462027818	25.339606497250383
42	18.34816577183943	32.38105656966303	24.43340806337044	24.837369595127097
43	18.615326457687807	26.129130833221243	25.50822089653661	29.747321812554343
44	18.658448160797033	26.379140323356793	25.040178306153372	29.922233209692806
45	18.145663050055827	26.05543670646927	32.557061766485695	23.24183847698921
46	17.235052534482232	30.256324728610906	27.853793713682904	24.654829023223957
47	19.93837126433137	30.59966576913242	26.698461552992864	22.763501413543345
48	23.938119659790047	27.133084587242724	24.4387876695336	24.49000808343363
49	18.092193400310936	30.084581283828033	23.299565846599133	28.523659469261897
50	16.25852170137239	30.455606742213693	29.682056290378938	23.60381526603498
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	123164.0
1	118441.0
2	95848.0
3	76996.5
4	71080.5
5	46527.5
6	18654.0
7	11550.5
8	13381.0
9	13309.0
10	11633.0
11	10324.5
12	9451.0
13	8364.0
14	8218.5
15	8755.0
16	8721.5
17	9341.0
18	12113.5
19	15866.0
20	18388.0
21	21673.0
22	26922.5
23	33476.0
24	39832.0
25	48462.0
26	57925.5
27	67650.5
28	79158.0
29	91074.0
30	114843.5
31	169256.0
32	245341.5
33	267546.0
34	263237.0
35	294438.5
36	299135.0
37	291576.0
38	310098.0
39	335992.5
40	365284.0
41	396035.0
42	420366.0
43	435297.5
44	452001.0
45	481297.0
46	516736.0
47	534903.5
48	605039.0
49	712062.5
50	813461.0
51	843178.0
52	704133.5
53	527809.5
54	428210.5
55	395074.5
56	381195.5
57	360486.5
58	324757.0
59	263096.5
60	212729.5
61	181560.5
62	143847.0
63	108516.0
64	88632.0
65	78095.5
66	59316.0
67	39820.0
68	28044.5
69	21141.0
70	16432.0
71	11785.0
72	8479.0
73	5506.0
74	3224.5
75	1854.5
76	1517.0
77	1455.5
78	1106.5
79	842.5
80	670.0
81	338.0
82	38.5
83	22.0
84	25.5
85	23.5
86	28.5
87	31.5
88	22.5
89	18.5
90	13.0
91	6.5
92	6.0
93	6.0
94	3.5
95	1.0
96	1.0
97	1.0
98	1.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	42435.0
25	49643.0
26	35131.0
27	46313.0
28	44677.0
29	42728.0
30	70937.0
31	120703.0
32	136958.0
33	25082.0
34	23542.0
35	21956.0
36	26072.0
37	31833.0
38	17987.0
39	19986.0
40	20908.0
41	25197.0
42	33614.0
43	57769.0
44	191002.0
45	469278.0
46	715887.0
47	762549.0
48	1172293.0
49	1143807.0
50	1367978.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.417873274007293
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.72207665321518	6.754694288686176
2	11.482657827224264	2.1628443253117213
3	4.5342925728940076	1.281101785163647
4	2.40632151561971	0.9064972436249437
5	1.4522316499850354	0.683846682203079
6	0.9523630588654729	0.5381540759544582
7	0.7023170020747753	0.46300327666046653
8	0.5311914076726324	0.4002154689361918
9	0.4433651326035549	0.3758000969676348
>10	3.5293962668422845	6.781235264411962
>50	0.564190537868713	3.796347329332364
>100	1.3331084266095314	33.5568402639798
>500	0.2620018460078526	15.706890296964456
>1k	0.07309471790798784	11.879135936595318
>5k	0.0066449743552716226	4.370634201412734
>10k+	0.004746410253765445	10.342759463795046
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	80057	1.1919869153465505	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	41519	0.618185851809004	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	36774	0.5475364655801996	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	30259	0.4505331460268468	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	20685	0.30798367842841223	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	20295	0.3021768795602913	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	16858	0.25100260338149255	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	16227	0.24160750059743027	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	13219	0.19682070317356445	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	12988	0.19338129153629288	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA	12818	0.19085012279890684	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	12153	0.18094878626736735	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	12059	0.17954919884787154	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT	10718	0.15958274427825583	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	10435	0.15536909279190145	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT	9812	0.146093103830775	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9234	0.13748713012366248	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTA	8913	0.132707687978363	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCC	8633	0.1285387041756095	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT	8315	0.12380392971391094	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	7967	0.11862247841620305	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	7782	0.11586797126081237	No Hit
AATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCAT	7683	0.1143939377019817	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6879	0.10242299849693244	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6822	0.10157431250851477	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	6761	0.1006660696086292	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.055849493729029455	0.0	0.0	0.0	0.0
2	0.08498771266470277	0.0	0.0	0.0	0.0
3	0.11886070606207468	0.0	0.0	0.0	0.0
4	0.17150901579970415	0.0	0.0	0.0	0.0
5	0.20414620328411698	0.0	0.0	0.0	0.0
6	0.2776989889469817	0.0	0.0	0.0	0.0
7	0.3120335484082299	0.0	0.0	0.0	0.0
8	0.3569841273386324	0.0	0.0	0.0	0.0
9	0.40130935869862194	0.0	0.0	0.0	0.0
10	0.43537591205826454	0.0	0.0	0.0	0.0
11	0.4734178892583899	0.0	0.0	0.0	0.0
12	0.5091222576833999	0.0	0.0	0.0	0.0
13	0.5439332724363913	0.0	0.0	0.0	0.0
14	0.5796376408614013	0.0	0.0	0.0	0.0
15	0.6165331475157696	0.0	0.0	0.0	0.0
16	0.6582527639990382	0.0	0.0	0.0	0.0
17	0.7016548632312751	0.0	0.0	0.0	0.0
18	0.7347238383238303	0.0	0.0	0.0	0.0
19	0.7712917819651249	0.0	0.0	0.0	0.0
20	0.8096762114061908	0.0	0.0	0.0	0.0
21	0.8411669283448464	0.0	0.0	0.0	0.0
22	0.868265323062744	0.0	0.0	0.0	0.0
23	0.8974779881377521	0.0	0.0	0.0	0.0
24	0.9285369174682655	0.0	0.0	0.0	0.0
25	0.9598489636725174	0.0	0.0	0.0	0.0
26	0.9919203604979852	0.0	0.0	0.0	0.0
27	1.0173958293783822	0.0	0.0	0.0	0.0
28	1.0470551712893996	0.0	0.0	0.0	0.0
29	1.0805857124458311	0.0	0.0	0.0	0.0
30	1.1122253216631566	0.0	0.0	0.0	0.0
31	1.1397852824449304	0.0	0.0	0.0	0.0
32	1.1684768245445945	0.0	0.0	0.0	0.0
33	1.1959027822755655	0.0	0.0	0.0	0.0
34	1.2263661424913996	0.0	0.0	0.0	0.0
35	1.2528391896388842	0.0	0.0	0.0	0.0
36	1.2750836960721472	0.0	0.0	0.0	0.0
37	1.2927869880059826	0.0	0.0	0.0	0.0
38	1.3065893022386699	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCGG	40	0.0	195.47218	44
CACGCTC	110	0.0	177.70198	44
CCGACGC	195	0.0	170.41165	44
GAGTACC	110	0.0	168.81688	44
ATATCGA	95	0.0	154.32014	44
CGTAACA	135	0.0	152.03392	44
ATATCGT	115	0.0	144.47943	44
CTGGCGT	130	0.0	135.32689	44
CTATCAT	11215	0.0	118.8694	44
GATGCGT	55	5.52609E-9	106.621185	44
AACGTCG	20	9.839001E-6	106.60704	43
GTACCTG	280	0.0	104.71723	44
GTAACCC	75	0.0	99.4999	43
GTACGGT	65	0.0	98.406494	43
TCACGCT	110	0.0	96.91549	43
CCGGACT	120	0.0	88.839195	43
AATATCG	200	0.0	87.9508	43
TCGTGCG	165	0.0	87.22394	43
CGCGTGG	110	0.0	87.22394	43
ACCTCGA	80	0.0	86.61822	43
>>END_MODULE