FastQCFastQC Report
Tue 24 May 2016
ERR841123.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841123.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11805354
Sequences flagged as poor quality0
Sequence length24-50
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA6537595.537817840955891No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA6497825.5041297363891No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA5787354.902309579196016No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA5767504.885495174477614No Hit
AAAAAAAAAAAAAAAAAAAAAAAA4475713.7912543749217513No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA4153133.5180054744652303No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2558482.1672200596441242No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1422881.2052836365601574No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA704980.5971697248553496No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAG520180.44063058168353103No Hit
AGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA481470.40784037479943425No Hit
AGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA472260.4000388298394102No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAG465880.39463450227752594No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAG456270.38649412800327715No Hit
AGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA418660.35463570173329834No Hit
AAAAAAAAAAAAAAAAAAAAAAAAG398530.33758411649493947No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAG388530.32911338363932163No Hit
AGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA387520.3282578396209042No Hit
AGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA380600.32239609248481665No Hit
AGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA363390.30781796124029825No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA347090.29401066668564113No Hit
AAAAAAAAAAAAAAAAAAAAAAAG328900.2786024036212722No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA328500.27826357430704746No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA327440.27736567662435196No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA309640.2622877721413521No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA282560.23934902756833892Illumina Single End Adapter 1 (100% over 32bp)
AGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA282380.2391965543769378No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA259420.21974775174043912No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA254290.21540226578550714No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA251470.2130135191202229No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA251150.21274245566884312Illumina Single End Adapter 1 (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAG247750.20986240649793303No Hit
AGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAA246690.2089645088152376No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA239830.2031535860762837Illumina Single End Adapter 1 (100% over 32bp)
AGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA219960.18632223989217095No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA212120.17968118533336655Illumina Single End Adapter 1 (100% over 32bp)
AGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAA209160.17717384840810366No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA198910.1684913472310953No Hit
CAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA187290.15864835565286733No Hit
TGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA179580.15211742062118597No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA179450.1520073010940629No Hit
AGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAA177580.1504232740500624No Hit
CAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAA174800.1480684103162006No Hit
AGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA172790.1463657930122214No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG170160.1441379902711939Illumina Single End Adapter 1 (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA167020.14147818015452987No Hit
ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA159170.13482865486286985Illumina Single End Adapter 1 (100% over 32bp)
CAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA158150.1339646401115968No Hit
AGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAA149600.12672216352004353No Hit
TGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA148930.12615462441871714No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG140850.11931027227137789No Hit
CAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAA140850.11931027227137789No Hit
TAAAAAAAAAAAAAAAAAAAAAAA136520.11564244494489534No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT136450.11558314981490601No Hit
TGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA131170.11111060286713977No Hit
TGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA129030.10929786603603754No Hit
AGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAA127830.10828137809336338No Hit
AGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA121320.10276693100435615No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGCG207.5477146E-8283.459644
GCCGGCC750.0283.4595644
CGATTGC500.0283.4595644
GCGACGA1050.0269.961544
ACGAATT950.0268.5406544
TCGATCA1300.0261.65544
CTACCGC1150.0246.4865944
CTAGCTA700.0242.9653644
GATATCC1050.0242.9653544
TATCCGG1150.0234.1622644
TTTGTCG252.2966196E-7226.7676744
CTACGAT500.0226.7676744
TCGTAGG3300.0223.3317944
ACGCCCC700.0222.7182544
TCCGATG1250.0215.4292944
TTCAGCG1900.0208.8649644
CAATCCC1400.0202.4711344
ACGAATG2750.0200.9986344
CTATACG1600.0194.8784644
GCACCGA950.0193.9460344
Produced by FastQC (version 0.11.5)