##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR841123.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11805354 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 28 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.06686118857596 35.0 33.0 35.0 24.0 39.0 2 34.375891481102556 35.0 33.0 35.0 31.0 39.0 3 34.39680970176752 35.0 33.0 35.0 30.0 39.0 4 34.82365077743539 35.0 34.0 35.0 32.0 39.0 5 35.4437561126926 35.0 35.0 35.0 33.0 41.0 6 35.20972594299163 35.0 34.0 36.0 32.0 41.0 7 35.499234669286494 35.0 35.0 36.0 33.0 41.0 8 35.30504201737618 35.0 34.0 36.0 32.0 41.0 9 35.45769046824009 35.0 35.0 36.0 33.0 41.0 10 35.47508876057423 35.0 35.0 36.0 33.0 41.0 11 35.47186539260068 35.0 35.0 36.0 33.0 41.0 12 35.149008746370505 35.0 35.0 35.0 33.0 39.0 13 34.80103256539363 35.0 35.0 35.0 33.0 37.0 14 34.514691130820815 35.0 35.0 35.0 33.0 36.0 15 34.37180062537727 35.0 35.0 35.0 33.0 35.0 16 34.392085574053944 35.0 35.0 35.0 33.0 35.0 17 34.325242936382935 35.0 35.0 35.0 33.0 35.0 18 34.27049642052242 35.0 35.0 35.0 33.0 35.0 19 34.18125318393671 35.0 35.0 35.0 33.0 35.0 20 34.107632943493265 35.0 34.0 35.0 32.0 35.0 21 33.962724878898165 35.0 34.0 35.0 31.0 35.0 22 34.00801509213532 35.0 34.0 35.0 32.0 35.0 23 33.91983679608422 35.0 34.0 35.0 31.0 35.0 24 33.43670075458982 35.0 34.0 35.0 30.0 35.0 25 33.315859741640566 35.0 33.0 35.0 28.0 35.0 26 33.138178723892004 35.0 33.0 35.0 25.0 35.0 27 32.984714605326275 35.0 33.0 35.0 24.0 35.0 28 33.01938258973947 35.0 33.0 35.0 24.0 35.0 29 33.21813425980906 35.0 34.0 35.0 25.0 35.0 30 33.58678432443548 35.0 34.0 35.0 30.0 35.0 31 33.89935542479037 35.0 34.0 35.0 31.0 35.0 32 33.99735097367877 35.0 34.0 35.0 32.0 35.0 33 34.03198097390892 35.0 34.0 35.0 31.0 35.0 34 34.19768661799215 35.0 34.0 35.0 32.0 36.0 35 34.21991118344742 35.0 34.0 35.0 32.0 35.0 36 34.23829981107361 35.0 34.0 35.0 32.0 35.0 37 34.21531198202411 35.0 34.0 35.0 32.0 35.0 38 34.12035148457773 35.0 34.0 35.0 32.0 35.0 39 34.029563272309424 35.0 34.0 35.0 31.0 35.0 40 33.9464284469828 35.0 34.0 35.0 31.0 35.0 41 33.837295763284224 35.0 34.0 35.0 31.0 35.0 42 33.73040492717608 35.0 34.0 35.0 31.0 35.0 43 33.68838490479687 35.0 34.0 35.0 31.0 35.0 44 33.55457406450881 35.0 34.0 35.0 30.0 35.0 45 33.4200108522567 35.0 34.0 35.0 29.0 35.0 46 33.217344013792044 35.0 33.0 35.0 27.0 35.0 47 33.110940258344314 35.0 33.0 35.0 27.0 35.0 48 33.04410487714378 35.0 33.0 35.0 27.0 35.0 49 33.235618940686614 35.0 33.0 35.0 29.0 35.0 50 32.913588570667436 34.0 33.0 35.0 27.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 4.0 18 30.0 19 276.0 20 1129.0 21 3312.0 22 6781.0 23 11322.0 24 20152.0 25 33376.0 26 53595.0 27 84932.0 28 133073.0 29 202797.0 30 308287.0 31 479401.0 32 808282.0 33 1934604.0 34 4608015.0 35 1105074.0 36 1097725.0 37 547702.0 38 142395.0 39 118147.0 40 104942.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 71.34203684192782 12.557488746207865 16.10047441186431 2 23.778355142929218 57.10295515068841 11.82424516875987 7.294444537622506 3 15.609476852621276 52.34834126956295 18.769509156608095 13.272672721207682 4 20.607158413038697 56.70877806798509 13.252232842827077 9.431830676149143 5 16.294411840593686 53.52823811975481 12.260496381557045 17.916853658094453 6 11.297518058331839 62.74387028123003 17.012153976915897 8.94645768352224 7 25.929252100360568 52.83641642597079 11.207635112000878 10.026696361667765 8 11.43249918638611 53.49665922766908 20.67331483664107 14.397526749303749 9 20.078279736465333 58.607331893647576 13.130508411691846 8.18387995819524 10 15.196282974657091 51.70564982634151 15.422400717504955 17.675666481496446 11 13.369764261198775 54.61733718446732 21.905281281696425 10.107617272637484 12 14.647371014880198 53.52546819011103 19.99485995930321 11.832300835705562 13 14.520216843984517 59.28967483736617 12.069574533724275 14.120533784925044 14 14.481632655827179 59.57013233148282 15.756842192110462 10.191392820579544 15 21.287959683377558 54.59527939611129 15.151820097897954 8.964940822613197 16 21.929609226457757 59.49883417303708 10.016175711461088 8.555380889044073 17 22.966892818292443 57.52448422978252 11.3980317743966 8.110591177528434 18 24.702215621827182 54.5136469435817 10.120611376838001 10.663526057753117 19 21.33166866491255 55.15127288855548 11.252081047294304 12.264977399237669 20 18.782867502321405 58.767428744618755 10.882274263016594 11.567429490043246 21 15.36823038089328 63.35535554461137 11.774073018056045 9.502341056439308 22 13.797671802133168 63.85206237779909 11.903963235664088 10.446302584403652 23 12.84950031993958 65.02203152908417 11.243635726637253 10.88483242433899 24 11.439267301937749 66.05363125917275 12.776973905229779 9.730127533659727 25 11.71657627079468 63.825581157594634 13.582930248642727 10.87491232296796 26 11.972480280738482 63.02319533021016 13.230998473203638 11.773325915847721 27 13.091472786978661 59.21330420971868 15.538443857027929 12.15677914627473 28 15.899991529806165 55.099602792489165 14.969217532409637 14.031188145295031 29 14.675584035986166 52.27179637569286 17.395571530017957 15.657048058303017 30 15.876247864950022 49.23754470387595 20.69077989248365 14.19542753869038 31 16.229795796739822 47.967054377795684 20.198404511944602 15.604745313519896 32 17.767424526917704 47.043884544553094 19.686810268566425 15.501880659962778 33 15.447727030527899 47.867249637144404 21.329710030724524 15.355313301603168 34 14.411779991496193 49.04854920127126 19.736947279131236 16.802723528101314 35 14.424772895485896 48.713749504426715 19.353011428564415 17.508466171522976 36 15.794372940194771 49.0569310975806 19.814846568497575 15.33384939372705 37 14.64332029227991 50.33769587930298 18.436775614505315 16.58220821391179 38 16.054114157465218 48.92807785918821 20.141058181169182 14.876749802177391 39 15.2824343202559 51.39165441811863 19.302013255585802 14.02389800603967 40 13.130450073457734 50.73199232117519 19.43575710084657 16.701800504520506 41 13.624830224677954 53.09107231870723 18.67646754904368 14.607629907571132 42 14.698993568911451 52.17392779564379 18.73694968115997 14.390128954284782 43 14.872401649599995 47.91531861785289 19.511774609567393 17.700505122979724 44 13.17880395037941 48.69735825168709 19.02617426280448 19.09766353512902 45 13.950101971133613 47.25300325413549 21.374610189714097 17.422284585016794 46 14.991222950063404 45.155707356586475 22.68323242342958 17.169837269920546 47 18.408245221824533 43.69284037452301 20.81520762609974 17.083706777552717 48 18.381931293038228 42.18383216315936 22.131081973216908 17.303154570585505 49 16.235782159378477 40.38699681458742 23.170313649512174 20.20690737652193 50 17.16682218967921 40.20822373762582 22.43302844836424 20.191925624330732 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4138827.0 1 4108669.0 2 2276554.5 3 473006.0 4 466542.0 5 367083.5 6 167626.0 7 62609.0 8 56524.0 9 41232.0 10 25385.0 11 18175.5 12 16802.0 13 16531.5 14 19711.0 15 24270.5 16 27060.0 17 31651.0 18 42462.0 19 63125.0 20 97919.5 21 163894.5 22 268056.5 23 400699.5 24 510327.5 25 565335.0 26 568010.0 27 515351.0 28 427357.5 29 344208.0 30 286972.5 31 259019.0 32 259048.0 33 265952.5 34 285143.5 35 320247.0 36 349327.5 37 347366.0 38 382163.0 39 455734.0 40 489826.5 41 516594.5 42 534922.5 43 517385.0 44 487529.5 45 463230.5 46 453129.0 47 452756.0 48 474151.0 49 490907.0 50 518014.5 51 543617.5 52 512582.5 53 450807.0 54 390483.0 55 330281.5 56 273809.5 57 230960.0 58 188351.5 59 148302.5 60 112132.5 61 79999.0 62 56123.5 63 44200.5 64 37714.0 65 31512.5 66 21732.0 67 12564.5 68 6914.5 69 4306.5 70 3417.5 71 3221.5 72 2920.0 73 1873.5 74 816.5 75 505.0 76 297.5 77 162.0 78 127.5 79 96.5 80 70.5 81 31.5 82 4.0 83 1.5 84 3.0 85 5.0 86 3.0 87 1.0 88 1.0 89 1.0 90 1.5 91 1.5 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 556684.0 25 709609.0 26 767838.0 27 798583.0 28 720580.0 29 544395.0 30 378497.0 31 336410.0 32 330328.0 33 81848.0 34 62878.0 35 55928.0 36 57641.0 37 72705.0 38 70824.0 39 90391.0 40 121780.0 41 153943.0 42 187169.0 43 222584.0 44 295035.0 45 572549.0 46 866037.0 47 638333.0 48 1035817.0 49 740617.0 50 1336351.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 5.227614878832886 #Duplication Level Percentage of deduplicated Percentage of total 1 71.46995056096142 3.7361737694193264 2 11.690579484540596 1.222276945111258 3 4.541603602052104 0.7122526369154581 4 2.3354656270933924 0.48835659444784785 5 1.415139118573921 0.36989011559377427 6 0.9598544019711198 0.301064949195448 7 0.7164105270915118 0.26215828315432677 8 0.5715456502930729 0.23902564363234277 9 0.474561374795479 0.2232741693420212 >10 4.003976704491633 4.241581415272853 >50 0.584754938103477 2.168945646016058 >100 0.9996105729731429 12.879557062833689 >500 0.1398914104927468 4.774715834437393 >1k 0.07025749888045828 7.867597432832469 >5k 0.012263882940671711 4.442545531796302 >10k+ 0.014134644745180955 56.07058396999947 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAA 653759 5.537817840955891 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAA 649782 5.5041297363891 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAA 578735 4.902309579196016 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 576750 4.885495174477614 No Hit AAAAAAAAAAAAAAAAAAAAAAAA 447571 3.7912543749217513 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAA 415313 3.5180054744652303 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 255848 2.1672200596441242 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 142288 1.2052836365601574 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 70498 0.5971697248553496 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAG 52018 0.44063058168353103 No Hit AGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 48147 0.40784037479943425 No Hit AGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 47226 0.4000388298394102 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAG 46588 0.39463450227752594 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAG 45627 0.38649412800327715 No Hit AGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 41866 0.35463570173329834 No Hit AAAAAAAAAAAAAAAAAAAAAAAAG 39853 0.33758411649493947 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAG 38853 0.32911338363932163 No Hit AGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 38752 0.3282578396209042 No Hit AGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 38060 0.32239609248481665 No Hit AGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 36339 0.30781796124029825 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAA 34709 0.29401066668564113 No Hit AAAAAAAAAAAAAAAAAAAAAAAG 32890 0.2786024036212722 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAA 32850 0.27826357430704746 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 32744 0.27736567662435196 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAA 30964 0.2622877721413521 No Hit ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA 28256 0.23934902756833892 Illumina Single End Adapter 1 (100% over 32bp) AGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 28238 0.2391965543769378 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 25942 0.21974775174043912 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA 25429 0.21540226578550714 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAA 25147 0.2130135191202229 No Hit ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA 25115 0.21274245566884312 Illumina Single End Adapter 1 (100% over 32bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAG 24775 0.20986240649793303 No Hit AGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAA 24669 0.2089645088152376 No Hit ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA 23983 0.2031535860762837 Illumina Single End Adapter 1 (100% over 32bp) AGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 21996 0.18632223989217095 No Hit ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA 21212 0.17968118533336655 Illumina Single End Adapter 1 (100% over 32bp) AGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAA 20916 0.17717384840810366 No Hit TAAAAAAAAAAAAAAAAAAAAAAAA 19891 0.1684913472310953 No Hit CAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 18729 0.15864835565286733 No Hit TGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 17958 0.15211742062118597 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 17945 0.1520073010940629 No Hit AGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAA 17758 0.1504232740500624 No Hit CAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAA 17480 0.1480684103162006 No Hit AGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 17279 0.1463657930122214 No Hit ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG 17016 0.1441379902711939 Illumina Single End Adapter 1 (100% over 32bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16702 0.14147818015452987 No Hit ATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA 15917 0.13482865486286985 Illumina Single End Adapter 1 (100% over 32bp) CAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 15815 0.1339646401115968 No Hit AGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAA 14960 0.12672216352004353 No Hit TGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14893 0.12615462441871714 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG 14085 0.11931027227137789 No Hit CAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAA 14085 0.11931027227137789 No Hit TAAAAAAAAAAAAAAAAAAAAAAA 13652 0.11564244494489534 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT 13645 0.11558314981490601 No Hit TGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 13117 0.11111060286713977 No Hit TGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 12903 0.10929786603603754 No Hit AGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAA 12783 0.10828137809336338 No Hit AGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 12132 0.10276693100435615 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.5016113875111242 0.0 0.0 0.0 0.0 2 0.8860555981633418 0.0 0.0 0.0 0.0 3 1.187401919501948 0.0 0.0 0.0 0.0 4 1.5111956829079416 0.0 0.0 0.0 0.0 5 1.7682824250759444 0.0 0.0 0.0 0.0 6 2.1494061084487597 0.0 0.0 0.0 0.0 7 2.381834547274059 0.0 0.0 0.0 0.0 8 2.660996019263802 0.0 0.0 0.0 0.0 9 2.9377263909239826 0.0 0.0 0.0 0.0 10 3.1720183909775175 0.0 0.0 0.0 0.0 11 3.411841779585771 0.0 0.0 0.0 0.0 12 3.619789800458334 0.0 0.0 0.0 0.0 13 3.8067304038489653 0.0 0.0 0.0 0.0 14 4.010934360799346 0.0 0.0 0.0 0.0 15 4.146322084030686 0.0 0.0 0.0 0.0 16 4.285199749198542 0.0 0.0 0.0 0.0 17 4.4918009235470615 0.0 0.0 0.0 0.0 18 4.587418556021277 0.0 0.0 0.0 0.0 19 4.702425696002001 0.0 0.0 0.0 0.0 20 4.828842913139241 0.0 0.0 0.0 0.0 21 4.9389285573308515 0.0 0.0 0.0 0.0 22 5.044550125307551 0.0 0.0 0.0 0.0 23 5.154263057253514 0.0 0.0 0.0 0.0 24 5.249474094550659 0.0 0.0 0.0 0.0 25 5.3420761461282735 0.0 8.470732855617883E-6 0.0 0.0 26 5.435398209998616 0.0 8.470732855617883E-6 0.0 0.0 27 5.505341051187453 0.0 8.470732855617883E-6 0.0 0.0 28 5.574818002069231 0.0 8.470732855617883E-6 0.0 0.0 29 5.640076527988911 0.0 8.470732855617883E-6 0.0 0.0 30 5.695669947720331 0.0 8.470732855617883E-6 0.0 0.0 31 5.744291954311578 0.0 8.470732855617883E-6 0.0 0.0 32 5.788322823695079 0.0 8.470732855617883E-6 0.0 0.0 33 5.82617005809398 0.0 8.470732855617883E-6 0.0 0.0 34 5.859570157743681 0.0 8.470732855617883E-6 0.0 0.0 35 5.885304244159048 0.0 8.470732855617883E-6 0.0 0.0 36 5.904448100412745 0.0 8.470732855617883E-6 0.0 0.0 37 5.9150958116122565 0.0 8.470732855617883E-6 0.0 0.0 38 5.923685134727853 0.0 8.470732855617883E-6 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCCGCG 20 7.5477146E-8 283.4596 44 GCCGGCC 75 0.0 283.45956 44 CGATTGC 50 0.0 283.45956 44 GCGACGA 105 0.0 269.9615 44 ACGAATT 95 0.0 268.54065 44 TCGATCA 130 0.0 261.655 44 CTACCGC 115 0.0 246.48659 44 CTAGCTA 70 0.0 242.96536 44 GATATCC 105 0.0 242.96535 44 TATCCGG 115 0.0 234.16226 44 TTTGTCG 25 2.2966196E-7 226.76767 44 CTACGAT 50 0.0 226.76767 44 TCGTAGG 330 0.0 223.33179 44 ACGCCCC 70 0.0 222.71825 44 TCCGATG 125 0.0 215.42929 44 TTCAGCG 190 0.0 208.86496 44 CAATCCC 140 0.0 202.47113 44 ACGAATG 275 0.0 200.99863 44 CTATACG 160 0.0 194.87846 44 GCACCGA 95 0.0 193.94603 44 >>END_MODULE