FastQCFastQC Report
Tue 24 May 2016
ERR841122.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841122.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3853524
Sequences flagged as poor quality0
Sequence length24-50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA215720.5597992902081316No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA205070.5321622494111883No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA198370.5147755664685104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA174020.45158665159474803No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA151800.39392514488037444No Hit
AAAAAAAAAAAAAAAAAAAAAAAA141850.3681046231968453No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG116700.30283968647918114No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA96790.25117269283907406No Hit
AAGCAGTAGGCAGAGGCTGGTGGG85510.2219007848400581No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC76500.1985195888231136No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC74910.19439349540835868No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA69950.18152215997616727No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56580.14682664490995773No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGA55660.14443921978947064No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG55220.14329740777532463No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC52170.13538257449544885No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA51110.1326318455522789No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA48800.12663733247801232No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA46690.12116182486472123No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC46490.12064281940374576No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG46050.11950100738959975No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA43310.11239063257423595No Hit
TGGGTTGTTCCGCTGGTGGATGGTTTGGGGTTGGTCGGGCGGGG42190.10948420199277337No Hit
CTGCCTTCGCTCACTAGAAGCAGGGAGCTCTCTTTTTCCCCGGTTCC40170.10424224683692122No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA38840.1007908605214344No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGGTATCATTTATT38760.1005832583370442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGCGA600.0177.182844
CGTACTT207.852577E-7177.182844
ATACGTC301.6007107E-10177.182844
CGCAACC650.0177.182844
TATCCGG500.0177.1827944
ATCTACG900.0167.3393144
AGCCGTA1400.0164.5268744
GCTATAC550.0161.0752744
CCCGAGA1250.0148.8335444
ACGACGT900.0147.6523344
ATGCATA850.0145.9152444
CCACGGC900.0137.8088444
AACCCGT2150.0127.7364344
ATTCTCG1400.0126.5591344
AATGTCG452.7102942E-9118.12186444
CACGACG750.0111.9423443
CGTCGTT153.4459966E-4111.9423343
GCGTGAC600.0111.9423343
CCGCAAC650.0111.9423343
TCCGGGC1700.0111.9423243
Produced by FastQC (version 0.11.5)