##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841122.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3853524
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.18261829950975	39.0	37.0	39.0	34.0	39.0
2	37.793970402156575	39.0	38.0	39.0	35.0	39.0
3	38.001100291577266	39.0	38.0	39.0	35.0	39.0
4	38.34511553580567	39.0	38.0	40.0	35.0	41.0
5	39.12851120169486	40.0	38.0	41.0	35.0	41.0
6	39.15543071744201	40.0	38.0	41.0	35.0	41.0
7	39.121297804295494	40.0	38.0	41.0	35.0	41.0
8	39.00619277316036	40.0	38.0	41.0	35.0	41.0
9	38.98466961669371	40.0	38.0	41.0	35.0	41.0
10	38.946764052851364	40.0	38.0	41.0	35.0	41.0
11	38.88543058250059	40.0	38.0	41.0	35.0	41.0
12	38.80698264757142	40.0	38.0	41.0	35.0	41.0
13	38.79395119895452	40.0	38.0	41.0	35.0	41.0
14	38.68390673056662	40.0	38.0	41.0	35.0	41.0
15	38.64585740221158	40.0	38.0	41.0	35.0	41.0
16	38.638605598408105	40.0	38.0	41.0	35.0	41.0
17	38.651806242805286	40.0	38.0	41.0	35.0	41.0
18	38.64151202898957	40.0	38.0	41.0	35.0	41.0
19	38.684304548252456	40.0	38.0	41.0	35.0	41.0
20	38.64280513109559	40.0	38.0	41.0	35.0	41.0
21	38.63902754984788	40.0	38.0	41.0	35.0	41.0
22	38.68113524140501	40.0	38.0	41.0	35.0	41.0
23	38.67653866953988	40.0	38.0	41.0	35.0	41.0
24	38.52584206041016	40.0	38.0	41.0	35.0	41.0
25	38.51835667087869	40.0	38.0	41.0	35.0	41.0
26	38.560314118505936	40.0	38.0	41.0	35.0	41.0
27	38.524570116756294	40.0	38.0	41.0	35.0	41.0
28	38.52506602248835	40.0	38.0	41.0	34.0	41.0
29	38.570545447148575	40.0	38.0	41.0	35.0	41.0
30	38.64079251119595	40.0	38.0	41.0	35.0	41.0
31	38.54464049656089	40.0	38.0	41.0	34.0	41.0
32	38.62024147337151	40.0	38.0	41.0	34.0	41.0
33	38.49784855779194	40.0	38.0	41.0	34.0	41.0
34	38.52247755121177	40.0	38.0	41.0	34.0	41.0
35	38.488645768740994	40.0	37.0	41.0	34.0	41.0
36	38.54191264414309	40.0	37.0	41.0	35.0	41.0
37	38.43117465788573	40.0	37.0	41.0	34.0	41.0
38	38.35516286989183	40.0	37.0	41.0	34.0	41.0
39	38.376573873630996	40.0	37.0	41.0	34.0	41.0
40	38.356977941917634	40.0	37.0	41.0	34.0	41.0
41	38.25143476836691	40.0	37.0	41.0	34.0	41.0
42	38.17859440059731	40.0	37.0	41.0	34.0	41.0
43	38.14865289757269	40.0	36.0	41.0	34.0	41.0
44	38.04818518617908	40.0	36.0	41.0	34.0	41.0
45	37.9249812328181	40.0	36.0	41.0	34.0	41.0
46	37.63187818165209	39.0	36.0	41.0	33.0	41.0
47	37.49643978603535	39.0	35.0	41.0	33.0	41.0
48	37.352409799775955	39.0	35.0	41.0	32.0	41.0
49	37.62442945524548	39.0	36.0	41.0	33.0	41.0
50	37.03967914228715	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	6.0
20	20.0
21	51.0
22	145.0
23	261.0
24	470.0
25	804.0
26	1489.0
27	2941.0
28	5648.0
29	10777.0
30	20382.0
31	38294.0
32	65851.0
33	117600.0
34	188789.0
35	160790.0
36	269021.0
37	411737.0
38	517017.0
39	835804.0
40	1205626.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	55.03806386050793	18.314509005263755	26.64742713422831
2	25.490356359529613	27.86600005605259	30.12730684952267	16.516336734895123
3	25.528217807907776	24.487248554829293	25.961431666183994	24.02310197107894
4	20.487143715726177	26.430327149902272	30.515107729963535	22.567421404408016
5	19.071244917639024	30.243745724692516	26.910043897481888	23.77496546018657
6	21.363017331668363	32.22499717142024	25.983619149640692	20.428366347270707
7	24.438747494501136	31.427882634181074	24.424137490774676	19.709232380543106
8	22.229341247128602	31.26725044400917	25.340441632126854	21.162966676735373
9	23.13015307547066	31.52322393736227	23.581272622150532	21.76535036501654
10	22.657391001068113	30.660844463405446	24.56663044008549	22.115134095440954
11	23.166223955008455	30.59542382504949	24.85979067471748	21.378561545224578
12	20.24910186104978	28.05486614330156	28.901934956159607	22.79409703948905
13	23.882166038151055	28.30271720119039	24.76564308409653	23.049473676562023
14	20.27876302314453	31.306746759589405	26.10895896846627	22.305531248799802
15	21.50672994381247	31.8048622507606	27.911542785253186	18.77686502017374
16	21.991169641086962	35.03484083659528	22.84698369596245	20.127005826355305
17	23.837349916595823	34.758496378898904	22.49374857922255	18.91040512528273
18	21.874808616736267	33.31031544113907	23.700669828447936	21.11420611367673
19	26.206687696767943	29.360761733934964	23.759654799087794	20.672895770209294
20	23.345073236860596	28.36569851387976	26.647920189416237	21.641308059843407
21	22.58916773322289	29.86949088678311	25.64234710877628	21.898994271217724
22	22.80595631427234	30.952681234111946	24.923109340956486	21.31825311065923
23	25.06007488210791	32.12511457045551	22.81589526885002	19.998915278586562
24	23.437845463009964	32.69316604749315	23.860289957970938	20.008698531525948
25	22.254173890775668	32.867526982834256	24.697059700176737	20.181239426213335
26	24.234664066236768	31.4314068157939	25.948297906450772	18.38563121151856
27	25.20798035677053	31.638742100069628	23.127131594307922	20.02614594885192
28	21.730435037453447	31.9808808859876	23.211314295266245	23.077369781292706
29	25.071964674086338	29.78433138275531	25.86915623756149	19.274547705596863
30	26.21792668726886	29.517019036794174	25.760781353449136	18.504272922487832
31	21.973133857851803	29.141259513019918	25.25446020047758	23.631146428650702
32	21.443262365161512	29.394210007423936	26.98530898251269	22.17721864490186
33	22.066110576097138	29.329618440230842	27.17660406288563	21.427666920786393
34	21.593837358568823	29.290782528559483	27.966851238720956	21.14852887415074
35	22.981040386563926	26.568435719871243	27.0525367896288	23.397987103936032
36	22.501316335461556	29.008023544098112	27.495371032251093	20.995289088189246
37	21.968471773105307	29.52733975578241	28.266105416313476	20.23808305479881
38	22.323571884855763	28.096724182108225	24.96808961593004	24.611614317105975
39	21.48315818414288	30.282169483200892	26.322590671700336	21.91208166095589
40	20.796048476129638	30.042126446139523	26.889293274142783	22.272531803588056
41	21.351424950543088	29.45194025118617	26.02095609991602	23.17567869835472
42	22.233918599063692	30.293421135793118	26.632861751950593	20.839798513192594
43	21.566160811399566	29.531141937036708	26.147009807356586	22.75568744420714
44	20.299332544317373	31.574487825927903	25.100485408294166	23.025694221460554
45	20.1869544386627	30.336284840984963	27.215200008198632	22.261560712153706
46	20.84066895316553	30.68766591649573	25.049688443769014	23.421976686569725
47	21.260773093733075	29.51083159202067	29.23826229814	19.990133016106256
48	20.643540413753026	32.08421108974334	25.164256739448764	22.10799175705487
49	19.64349218945172	29.926450058046406	27.11494658260959	23.315111169892283
50	21.387091153913165	33.97551798420865	23.242222881188724	21.39516798068946
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	181304.0
1	178371.5
2	121569.5
3	66661.5
4	62683.5
5	44819.0
6	19737.5
7	9985.0
8	10385.0
9	10920.5
10	11758.0
11	11873.5
12	11055.0
13	11038.0
14	13266.0
15	15512.0
16	16379.5
17	16115.5
18	15373.0
19	15411.5
20	15934.5
21	17802.5
22	20896.0
23	27321.5
24	33212.5
25	33754.5
26	37097.5
27	51551.5
28	66997.5
29	80053.0
30	91970.5
31	100322.5
32	117613.5
33	139396.0
34	157468.0
35	168551.5
36	166434.0
37	168667.5
38	182956.0
39	205175.0
40	236504.0
41	263626.0
42	268644.5
43	264380.0
44	267661.0
45	268565.0
46	281830.5
47	287986.0
48	289874.5
49	317911.5
50	340390.5
51	327365.0
52	290937.5
53	249587.0
54	216265.5
55	202165.5
56	204235.0
57	188206.0
58	150632.0
59	127849.0
60	120301.5
61	114384.0
62	100693.5
63	83605.0
64	73587.5
65	67067.0
66	51038.0
67	31372.5
68	17487.0
69	10230.0
70	7255.0
71	5735.0
72	4151.0
73	2614.5
74	1553.5
75	916.0
76	681.0
77	945.0
78	1361.5
79	1397.5
80	1357.5
81	813.0
82	166.5
83	33.0
84	14.0
85	205.0
86	401.5
87	402.0
88	398.5
89	395.0
90	202.0
91	9.0
92	9.0
93	9.0
94	4.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	42212.0
25	37577.0
26	42373.0
27	46254.0
28	47212.0
29	38603.0
30	40092.0
31	27383.0
32	33467.0
33	21660.0
34	17496.0
35	17823.0
36	21389.0
37	32371.0
38	15915.0
39	18459.0
40	22561.0
41	23860.0
42	24587.0
43	40217.0
44	119541.0
45	232464.0
46	445492.0
47	352060.0
48	731476.0
49	494303.0
50	866677.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.171907764280341
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.09642618023237	9.891631990731083
2	10.721012552499577	2.824323769624323
3	4.115269154648038	1.6261783719063567
4	2.2195224903629924	1.1694138209522855
5	1.3237480193136253	0.8718143406773934
6	0.8119918570543829	0.6417289107880224
7	0.584303715907242	0.5387476256579519
8	0.4508342636969964	0.4750677870715263
9	0.3597200495802791	0.4264379382630409
>10	2.769540473855834	7.203916656763451
>50	0.3392362043723625	3.164982714702172
>100	0.950575529676096	34.02564485981634
>500	0.20925516919240864	17.329066702745738
>1k	0.04249379715682019	10.978076453635357
>5k	0.0035709073241025364	3.1720850390668702
>10k+	0.0024996351268717757	5.660883017598088
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA	21572	0.5597992902081316	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	20507	0.5321622494111883	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	19837	0.5147755664685104	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	17402	0.45158665159474803	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15180	0.39392514488037444	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	14185	0.3681046231968453	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG	11670	0.30283968647918114	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9679	0.25117269283907406	No Hit
AAGCAGTAGGCAGAGGCTGGTGGG	8551	0.2219007848400581	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	7650	0.1985195888231136	No Hit
CTAGGCAGGTGCTGGGGGCTTCCGAGACAATC	7491	0.19439349540835868	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	6995	0.18152215997616727	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5658	0.14682664490995773	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGA	5566	0.14443921978947064	No Hit
CGGCAAGTTTGAATTTCGTGGAGGCTCGGG	5522	0.14329740777532463	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACC	5217	0.13538257449544885	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA	5111	0.1326318455522789	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4880	0.12663733247801232	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA	4669	0.12116182486472123	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	4649	0.12064281940374576	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGG	4605	0.11950100738959975	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	4331	0.11239063257423595	No Hit
TGGGTTGTTCCGCTGGTGGATGGTTTGGGGTTGGTCGGGCGGGG	4219	0.10948420199277337	No Hit
CTGCCTTCGCTCACTAGAAGCAGGGAGCTCTCTTTTTCCCCGGTTCC	4017	0.10424224683692122	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA	3884	0.1007908605214344	No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGGTATCATTTATT	3876	0.1005832583370442	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.17469723816433996	0.0	0.0	0.0	0.0
2	0.25745265891687713	0.0	0.0	0.0	0.0
3	0.33205969393209955	0.0	0.0	0.0	0.0
4	0.4271414943828039	0.0	0.0	0.0	0.0
5	0.4947938562209552	0.0	0.0	0.0	0.0
6	0.6263617405782343	0.0	0.0	0.0	0.0
7	0.6918342794802886	0.0	0.0	0.0	0.0
8	0.7690363418003884	0.0	0.0	0.0	0.0
9	0.8391020790320756	0.0	0.0	0.0	0.0
10	0.9036144578313253	0.0	0.0	0.0	0.0
11	0.9886535026121545	0.0	0.0	0.0	0.0
12	1.0521278704894532	0.0	0.0	0.0	0.0
13	1.123283519189189	0.0	0.0	0.0	0.0
14	1.189352914371365	0.0	0.0	0.0	0.0
15	1.2537095915323222	0.0	0.0	0.0	0.0
16	1.3216214561009612	0.0	0.0	0.0	0.0
17	1.3968512976693541	0.0	0.0	0.0	0.0
18	1.4410446126714145	0.0	0.0	0.0	0.0
19	1.4893899713612786	0.0	0.0	0.0	0.0
20	1.5458577655154087	0.0	0.0	0.0	0.0
21	1.5877674564891773	0.0	0.0	0.0	0.0
22	1.6265112141509952	0.0	0.0	0.0	0.0
23	1.6684468553978125	0.0	0.0	0.0	0.0
24	1.708384325619874	0.0	0.0	0.0	0.0
25	1.7519029335226666	0.0	0.0	0.0	0.0
26	1.7946430332339958	0.0	0.0	0.0	0.0
27	1.8292347472080102	0.0	0.0	0.0	0.0
28	1.8678228032315356	0.0	0.0	0.0	0.0
29	1.9075786215422559	0.0	0.0	0.0	0.0
30	1.9444020589984647	0.0	0.0	0.0	0.0
31	1.9779038615044306	0.0	0.0	0.0	0.0
32	2.012858879301128	0.0	0.0	0.0	0.0
33	2.0462828309879475	0.0	0.0	0.0	0.0
34	2.080666942777572	0.0	0.0	0.0	0.0
35	2.111443966613417	0.0	0.0	0.0	0.0
36	2.1373423391160924	0.0	0.0	0.0	0.0
37	2.154962574516209	0.0	0.0	0.0	0.0
38	2.169468777150473	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAGCGA	60	0.0	177.1828	44
CGTACTT	20	7.852577E-7	177.1828	44
ATACGTC	30	1.6007107E-10	177.1828	44
CGCAACC	65	0.0	177.1828	44
TATCCGG	50	0.0	177.18279	44
ATCTACG	90	0.0	167.33931	44
AGCCGTA	140	0.0	164.52687	44
GCTATAC	55	0.0	161.07527	44
CCCGAGA	125	0.0	148.83354	44
ACGACGT	90	0.0	147.65233	44
ATGCATA	85	0.0	145.91524	44
CCACGGC	90	0.0	137.80884	44
AACCCGT	215	0.0	127.73643	44
ATTCTCG	140	0.0	126.55913	44
AATGTCG	45	2.7102942E-9	118.121864	44
CACGACG	75	0.0	111.94234	43
CGTCGTT	15	3.4459966E-4	111.94233	43
GCGTGAC	60	0.0	111.94233	43
CCGCAAC	65	0.0	111.94233	43
TCCGGGC	170	0.0	111.94232	43
>>END_MODULE