FastQCFastQC Report
Tue 24 May 2016
ERR841121.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841121.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14596350
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG527990.3617274181559088No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA429550.2942859002421838No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG355880.24381437825209729No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC319720.21904106163527182No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGA239820.16430134930993023No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT229780.1574229173731789No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA218470.1496744049025955No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC212890.1458515313759947No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA210540.14424153983701404No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC203430.13937045905311946No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAA201830.1382742946010475No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGA200900.13763714901328072No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA199010.13634230475427075No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA170380.1167278120900088No Hit
TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG165920.11367225367985832No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC164300.11256238717213549No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA163330.11189783747306689No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCA161550.1106783545201369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG400.0200.9082244
ATATTCG850.0165.4538344
AATCGCA6700.0115.44726644
CATGACC18400.0114.6487144
AAATCGT1200.0108.8252944
ACTTCTA88750.097.45463644
CTATCAT86000.087.83893644
TACGATA1000.084.8862843
CCAGAAT106250.080.8360144
ACACGTC2000.080.3632944
TATCCCG1650.079.1456644
AACGACA45200.079.11137443
TCGCAAA17700.077.3328343
TCGTAAC3000.077.0148244
TAAACCG5350.073.22822644
CGACTAC1750.072.7596743
GGCGCAT1950.072.12089544
TTACGAC1003.5852281E-970.3178844
TACCGAC2050.068.60280644
ACGACAC21000.068.40446544
Produced by FastQC (version 0.11.5)