##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR841121.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14596350 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.60954039879833 39.0 38.0 39.0 35.0 39.0 2 38.03054243012808 39.0 38.0 39.0 35.0 39.0 3 38.16112589791283 39.0 38.0 39.0 35.0 39.0 4 38.60415699815365 39.0 38.0 40.0 35.0 41.0 5 39.31494949079735 40.0 39.0 41.0 36.0 41.0 6 39.32824055328901 40.0 39.0 41.0 36.0 41.0 7 39.35226018833475 40.0 39.0 41.0 36.0 41.0 8 39.28982211306251 40.0 39.0 41.0 36.0 41.0 9 39.27659223024934 40.0 39.0 41.0 36.0 41.0 10 39.24533242899766 40.0 39.0 41.0 36.0 41.0 11 39.168059686154415 40.0 39.0 41.0 35.0 41.0 12 39.096964720632215 40.0 38.0 41.0 35.0 41.0 13 39.06201790173571 40.0 38.0 41.0 35.0 41.0 14 39.054472316709315 40.0 38.0 41.0 35.0 41.0 15 39.00613920603438 40.0 38.0 41.0 35.0 41.0 16 39.05056771042076 40.0 38.0 41.0 35.0 41.0 17 39.09535877119965 40.0 38.0 41.0 35.0 41.0 18 39.07500895771888 40.0 39.0 41.0 35.0 41.0 19 39.08823150993228 40.0 39.0 41.0 35.0 41.0 20 39.123537665238224 40.0 39.0 41.0 35.0 41.0 21 39.15357825757809 40.0 39.0 41.0 35.0 41.0 22 39.16084418364865 40.0 38.0 41.0 35.0 41.0 23 39.114922566258 40.0 38.0 41.0 35.0 41.0 24 39.08057678803262 40.0 38.0 41.0 35.0 41.0 25 39.081213697153984 40.0 38.0 41.0 35.0 41.0 26 39.063280552800336 40.0 38.0 41.0 35.0 41.0 27 38.988387402299594 40.0 38.0 41.0 35.0 41.0 28 39.01765353238533 40.0 38.0 41.0 35.0 41.0 29 38.95350267271482 40.0 38.0 41.0 35.0 41.0 30 38.973635396247076 40.0 38.0 41.0 35.0 41.0 31 38.860265642525675 40.0 38.0 41.0 35.0 41.0 32 38.726480211425965 40.0 38.0 41.0 35.0 41.0 33 38.61630557008315 40.0 38.0 41.0 35.0 41.0 34 38.69783832936874 40.0 38.0 41.0 35.0 41.0 35 38.72063884891785 40.0 38.0 41.0 35.0 41.0 36 38.75311348980325 40.0 38.0 41.0 35.0 41.0 37 38.71695516649397 40.0 38.0 41.0 35.0 41.0 38 38.6470021049041 40.0 38.0 41.0 35.0 41.0 39 38.66984602459493 40.0 37.0 41.0 35.0 41.0 40 38.58900489570843 40.0 37.0 41.0 35.0 41.0 41 38.550678036822816 40.0 37.0 41.0 35.0 41.0 42 38.47754957474916 40.0 37.0 41.0 35.0 41.0 43 38.397787791983674 40.0 37.0 41.0 34.0 41.0 44 38.24106457720804 40.0 36.0 41.0 34.0 41.0 45 38.081061687401835 40.0 36.0 41.0 34.0 41.0 46 37.8081812073169 39.0 36.0 41.0 33.0 41.0 47 37.59210893305027 39.0 36.0 41.0 33.0 41.0 48 37.553730638453935 39.0 36.0 41.0 33.0 41.0 49 37.543763585263534 39.0 36.0 41.0 33.0 41.0 50 37.115522837490666 38.0 35.0 40.0 32.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 2.0 19 5.0 20 27.0 21 70.0 22 169.0 23 351.0 24 721.0 25 1318.0 26 2848.0 27 6238.0 28 13444.0 29 27651.0 30 54071.0 31 98792.0 32 172628.0 33 303662.0 34 595822.0 35 504785.0 36 782127.0 37 1277202.0 38 1949114.0 39 3375099.0 40 5430204.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 55.25924631842892 18.997249312328083 25.743504369243 2 26.68117714360097 22.4614372771275 29.524600328164237 21.332785251107296 3 23.28586256153079 24.144700558701317 27.98962069284444 24.57981618692344 4 21.62763293563117 23.61271139702734 33.12232852733732 21.637327140004178 5 18.46446543142635 25.02808578857043 31.757987442065993 24.749461337937223 6 22.042990199604695 24.430148633048674 31.54119351755747 21.98566764978916 7 25.05144779345521 24.316955951316597 27.140935918911236 23.490660336316957 8 21.84390618202496 23.19196922518301 29.058175502779804 25.905949090012232 9 21.439537966683453 26.084075813473916 28.727647665340996 23.74873855450164 10 24.90788450537292 22.702038523329463 30.34549733323742 22.0445796380602 11 24.4504002713007 22.954594813086835 28.968728483490736 23.626276432121728 12 22.73543728397853 26.285660456209943 28.671887149869658 22.30701510994187 13 24.767397328784252 24.35806896929712 28.05075241413093 22.8237812877877 14 21.350844560455183 27.067999876681498 25.747491667437405 25.83366389542591 15 20.717747930133218 28.078916989521353 28.03908511374419 23.16424996660124 16 24.470645058524905 28.253255094595563 25.741175019782343 21.534924827097186 17 22.958883556505565 31.921905133817702 23.565850366701262 21.55336094297547 18 21.73419382242821 28.15177767044501 25.412661384524217 24.70136712260257 19 25.846749358572517 24.875773737955036 26.90345189036985 22.374025013102592 20 24.964426038016356 25.566884871902907 26.846355424472556 22.62233366560818 21 22.31204376436575 27.16032432765726 26.752297663456957 23.77533424452003 22 26.38488389220593 28.363782726503544 24.51687579429104 20.73445758699949 23 26.16857639067301 26.649114333377867 26.17543426952629 21.00687500642284 24 23.666341242844958 28.26440171686757 24.365769524572926 23.703487515714546 25 23.758373373898664 27.920331712086476 29.0001718038399 19.321123110174955 26 24.58601449415509 29.930775627937305 26.21740127480045 19.265808603107153 27 25.817141420032442 27.15369079022792 26.5482168191964 20.48095097054324 28 24.668125863205734 27.79152314080711 25.918241480855286 21.622109515131864 29 25.10300532182454 28.83128231298443 25.734209738000324 20.331502627190705 30 27.398311138308955 28.517172818042276 24.54394201957566 19.54057402407311 31 25.812305056898644 27.342792201597028 26.008998157275155 20.835904584229176 32 24.281579650185147 28.549034925363337 26.443350039121547 20.72603538532997 33 23.451442597973625 25.178226517224584 29.08797849749653 22.28235238730526 34 22.376316551045612 27.675522314487033 26.552715814725786 23.39544531974157 35 22.204089941530064 25.86633782764774 29.014461891934683 22.915110338887516 36 23.24752567723889 24.745404968594624 28.41901998341154 23.588049370754952 37 20.15123752859521 28.675901467199242 26.501477292005216 24.671383712200328 38 23.015317608056904 27.406528116068348 27.487912582143153 22.0902416937316 39 23.499008288454064 28.492791015524954 26.206888100580304 21.801312595440677 40 19.729666771047345 27.781281743819264 29.417960605204268 23.071090879929123 41 21.07953725291003 29.744734163278792 26.821583140491384 22.354145443319798 42 22.464166238078732 29.36327344341365 25.943946163658776 22.22861415484884 43 21.07441370738216 25.61159595415902 29.253519852711367 24.06047048574745 44 21.399964578911884 27.813190181699554 25.44927804913277 25.337567190255793 45 20.976200413350433 27.27721532271718 27.467752375891784 24.278831888040603 46 20.383286317948457 28.565165409475807 27.5709919936839 23.480556278891836 47 24.317020588707422 27.148684664597745 27.678195775544427 20.856098971150406 48 22.401620430844314 29.181887440665726 25.38210549520325 23.034386633286708 49 19.062324434492343 30.703624655791412 26.118882851398805 24.115168058317444 50 20.945915558055653 28.866127392880397 27.58716841131492 22.600788637749027 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 183705.0 1 187378.5 2 166301.5 3 139742.5 4 133509.5 5 93777.0 6 42375.0 7 28238.5 8 31379.0 9 32925.0 10 33126.5 11 31929.0 12 29586.0 13 29125.5 14 33804.0 15 38741.0 16 39977.5 17 41549.5 18 45209.0 19 47610.0 20 49981.0 21 65213.5 22 85473.5 23 102440.0 24 117025.5 25 134422.0 26 159991.0 27 183750.0 28 197319.5 29 225512.5 30 277734.0 31 348795.0 32 435240.0 33 466942.5 34 499023.0 35 567602.5 36 582803.0 37 593440.0 38 643809.5 39 732275.5 40 825779.0 41 869794.0 42 950666.0 43 1089337.5 44 1180261.0 45 1211076.0 46 1241279.0 47 1275766.5 48 1343891.5 49 1451412.0 50 1570581.5 51 1582582.0 52 1467540.5 53 1328445.0 54 1140136.0 55 970510.5 56 864654.0 57 771829.5 58 673888.5 59 564639.0 60 442432.0 61 321702.0 62 238915.5 63 186019.0 64 144188.5 65 120322.5 66 84370.0 67 47942.5 68 32266.5 69 23278.5 70 17060.5 71 12990.0 72 9780.0 73 6441.0 74 4039.5 75 2985.5 76 1859.0 77 969.0 78 552.0 79 364.5 80 291.0 81 234.0 82 151.0 83 88.5 84 62.5 85 46.0 86 36.5 87 27.5 88 16.5 89 12.0 90 9.5 91 5.0 92 3.0 93 3.0 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 56512.0 25 62278.0 26 67692.0 27 84633.0 28 91019.0 29 89304.0 30 152047.0 31 254912.0 32 271242.0 33 49172.0 34 46052.0 35 52590.0 36 61870.0 37 76379.0 38 42251.0 39 45297.0 40 48307.0 41 54460.0 42 67136.0 43 128559.0 44 426440.0 45 1054880.0 46 1582644.0 47 1692318.0 48 2548952.0 49 2598850.0 50 2890554.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 10.299601055781292 #Duplication Level Percentage of deduplicated Percentage of total 1 68.48029936911703 7.053197636823769 2 12.925041434747717 2.6624554081468905 3 5.416347195004917 1.6735864586445204 4 2.7589315429550965 1.1366357693059448 5 1.7230192172669596 0.8873205274647116 6 1.14205067012425 0.7057599772660474 7 0.8099285515324779 0.5839358675129924 8 0.6114012683634252 0.5037751319313565 9 0.49588918565236506 0.4596714702086062 >10 3.6739028234005646 7.36884833789809 >50 0.4540841816021311 3.3478686563926248 >100 1.1750380821047421 33.32490791815471 >500 0.2674009995283441 17.325949604352157 >1k 0.05779645705670677 11.149069971041015 >5k 0.00443451077161433 3.026262697517544 >10k+ 0.00443451077161433 8.790754567339027 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG 52799 0.3617274181559088 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA 42955 0.2942859002421838 No Hit AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG 35588 0.24381437825209729 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC 31972 0.21904106163527182 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGA 23982 0.16430134930993023 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT 22978 0.1574229173731789 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAA 21847 0.1496744049025955 No Hit TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC 21289 0.1458515313759947 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAA 21054 0.14424153983701404 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC 20343 0.13937045905311946 No Hit AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAA 20183 0.1382742946010475 No Hit AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGA 20090 0.13763714901328072 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAA 19901 0.13634230475427075 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAA 17038 0.1167278120900088 No Hit TTGAGTTCCAGCCAGGAGGACCAGGGGCTGGGTTGGG 16592 0.11367225367985832 No Hit TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC 16430 0.11256238717213549 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 16333 0.11189783747306689 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCA 16155 0.1106783545201369 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.06714692371723067 0.0 0.0 0.0 0.0 2 0.10391639005641821 0.0 0.0 0.0 0.0 3 0.13228649628160466 0.0 0.0 0.0 0.0 4 0.18125764317791776 0.0 0.0 0.0 0.0 5 0.21063485049344527 0.0 0.0 0.0 0.0 6 0.29528615030469946 0.0 0.0 0.0 0.0 7 0.3324187211186358 0.0 0.0 0.0 0.0 8 0.3844865325920521 0.0 0.0 0.0 0.0 9 0.4332932548205545 0.0 0.0 0.0 0.0 10 0.4690213649302737 0.0 0.0 0.0 0.0 11 0.5156974175050612 0.0 0.0 0.0 0.0 12 0.5570365194038236 0.0 0.0 0.0 0.0 13 0.5993758713651015 0.0 0.0 0.0 0.0 14 0.6415028414637906 0.0 0.0 0.0 0.0 15 0.685280909268413 0.0 0.0 0.0 6.851027825449513E-6 16 0.7293809753808315 0.0 0.0 0.0 6.851027825449513E-6 17 0.7814693399377242 0.0 0.0 0.0 6.851027825449513E-6 18 0.8144159327503109 0.0 0.0 0.0 6.851027825449513E-6 19 0.8523637758754757 0.0 0.0 0.0 6.851027825449513E-6 20 0.8928464992960569 0.0 0.0 0.0 6.851027825449513E-6 21 0.9267248318929048 0.0 0.0 0.0 6.851027825449513E-6 22 0.9583560273630052 0.0 0.0 0.0 6.851027825449513E-6 23 0.9890554830488444 0.0 0.0 0.0 6.851027825449513E-6 24 1.0200289798477016 0.0 0.0 0.0 6.851027825449513E-6 25 1.0515437078447694 0.0 0.0 0.0 6.851027825449513E-6 26 1.0865250559215147 0.0 0.0 0.0 6.851027825449513E-6 27 1.1153747340944826 0.0 0.0 0.0 6.851027825449513E-6 28 1.1469853764811067 0.0 0.0 0.0 6.851027825449513E-6 29 1.178068489725171 0.0 0.0 0.0 6.851027825449513E-6 30 1.2076786319867638 0.0 0.0 0.0 6.851027825449513E-6 31 1.236089844378903 0.0 0.0 0.0 6.851027825449513E-6 32 1.2648230550788382 0.0 0.0 0.0 6.851027825449513E-6 33 1.294097496976984 0.0 0.0 0.0 6.851027825449513E-6 34 1.3235158104594642 0.0 0.0 0.0 6.851027825449513E-6 35 1.3529341239419443 0.0 0.0 0.0 6.851027825449513E-6 36 1.3755836219328805 0.0 0.0 0.0 6.851027825449513E-6 37 1.3915602188218288 0.0 0.0 0.0 6.851027825449513E-6 38 1.4059884834222254 0.0 0.0 0.0 6.851027825449513E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATACG 40 0.0 200.90822 44 ATATTCG 85 0.0 165.45383 44 AATCGCA 670 0.0 115.447266 44 CATGACC 1840 0.0 114.64871 44 AAATCGT 120 0.0 108.82529 44 ACTTCTA 8875 0.0 97.454636 44 CTATCAT 8600 0.0 87.838936 44 TACGATA 100 0.0 84.88628 43 CCAGAAT 10625 0.0 80.83601 44 ACACGTC 200 0.0 80.36329 44 TATCCCG 165 0.0 79.14566 44 AACGACA 4520 0.0 79.111374 43 TCGCAAA 1770 0.0 77.33283 43 TCGTAAC 300 0.0 77.01482 44 TAAACCG 535 0.0 73.228226 44 CGACTAC 175 0.0 72.75967 43 GGCGCAT 195 0.0 72.120895 44 TTACGAC 100 3.5852281E-9 70.31788 44 TACCGAC 205 0.0 68.602806 44 ACGACAC 2100 0.0 68.404465 44 >>END_MODULE