FastQCFastQC Report
Tue 24 May 2016
ERR841120.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841120.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12294993
Sequences flagged as poor quality0
Sequence length24-50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA1062960.8645470558624962No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA653720.5316961140197477No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT569810.4634488201823295No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC472490.38429464742273545No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA359880.29270451800989233No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA266100.2164295660843402No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT226050.1838553303771706No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC220360.17922743022301843No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG205060.16678334017758287No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT147830.12023593669390459No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACAC125820.10233434049128778No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT125210.10183820356790768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC1700.0111.3368944
AGTCCCG1700.0107.4976944
GGTGCGG900.0101.525644
GATCCGA1300.0100.4099344
ATTGACG1400.097.8996844
CTCGACT1400.093.2377944
GTTCGGC6950.088.661643
TATCGCA2250.087.7226543
ACGAACA6450.083.9862944
GTTACGC750.083.4434943
GGGTGCG850.079.290243
CGAGAGT1100.078.7753343
AATAACG1400.074.5902344
ACTCGAC1300.074.0622643
CGACTAG408.264033E-872.2107243
GAACACA217750.071.9654444
CCTTAGC314250.071.15752443
GCGTCGA1500.070.6060343
CGGAAGG2050.070.04204644
CTATCAT174300.068.97349544
Produced by FastQC (version 0.11.5)