##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841120.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12294993
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.827837071562385	39.0	38.0	39.0	35.0	39.0
2	38.098489930006465	39.0	38.0	39.0	37.0	39.0
3	38.19050470382537	39.0	38.0	39.0	35.0	39.0
4	38.51205828258706	39.0	39.0	39.0	35.0	41.0
5	39.529576877351616	41.0	39.0	41.0	36.0	41.0
6	39.47674577773245	41.0	39.0	41.0	36.0	41.0
7	39.51716401953218	41.0	39.0	41.0	36.0	41.0
8	39.46654170522911	41.0	39.0	41.0	36.0	41.0
9	39.403336057206374	41.0	39.0	41.0	36.0	41.0
10	39.40890556017397	41.0	39.0	41.0	36.0	41.0
11	39.29584506473489	40.0	39.0	41.0	36.0	41.0
12	39.26164797328474	40.0	39.0	41.0	36.0	41.0
13	39.17592925835745	40.0	39.0	41.0	35.0	41.0
14	39.19363231845679	40.0	39.0	41.0	35.0	41.0
15	39.11355549368755	40.0	38.0	41.0	35.0	41.0
16	39.12528750524705	40.0	38.0	41.0	35.0	41.0
17	39.21509422575515	40.0	39.0	41.0	35.0	41.0
18	39.20344306011398	40.0	39.0	41.0	35.0	41.0
19	39.131413820243736	41.0	39.0	41.0	35.0	41.0
20	39.14410890677205	41.0	39.0	41.0	35.0	41.0
21	39.21633212804595	41.0	39.0	41.0	35.0	41.0
22	39.22892611650938	41.0	39.0	41.0	35.0	41.0
23	39.184254029262156	41.0	39.0	41.0	35.0	41.0
24	39.1335302102246	40.0	39.0	41.0	35.0	41.0
25	39.11363108357901	40.0	38.0	41.0	35.0	41.0
26	39.054751913091714	40.0	38.0	41.0	35.0	41.0
27	39.02268340030018	40.0	38.0	41.0	35.0	41.0
28	38.92648507965962	40.0	38.0	41.0	35.0	41.0
29	38.90742946322231	40.0	38.0	41.0	35.0	41.0
30	38.914408644255964	40.0	38.0	41.0	35.0	41.0
31	38.8647591075072	40.0	38.0	41.0	35.0	41.0
32	38.758655146494256	40.0	38.0	41.0	35.0	41.0
33	38.60332411749157	40.0	37.0	41.0	35.0	41.0
34	38.56314186390491	40.0	37.0	41.0	35.0	41.0
35	38.608967306809014	40.0	37.0	41.0	35.0	41.0
36	38.58531148260657	40.0	37.0	41.0	35.0	41.0
37	38.5241678472676	40.0	37.0	41.0	35.0	41.0
38	38.353733954697724	40.0	37.0	41.0	35.0	41.0
39	38.27133049179961	39.0	36.0	41.0	34.0	41.0
40	38.23130457798251	39.0	36.0	41.0	35.0	41.0
41	38.124311076671894	39.0	36.0	41.0	34.0	41.0
42	37.95957363586706	39.0	36.0	41.0	34.0	41.0
43	37.84322613104599	39.0	36.0	41.0	34.0	41.0
44	37.65881804678666	39.0	35.0	41.0	34.0	41.0
45	37.46327315718118	38.0	35.0	40.0	34.0	41.0
46	37.10308718795978	38.0	35.0	40.0	33.0	41.0
47	36.831886689171846	37.0	35.0	40.0	33.0	41.0
48	36.55636709771663	37.0	35.0	40.0	32.0	41.0
49	36.853108711128826	37.0	35.0	39.0	33.0	41.0
50	36.24293997975231	37.0	35.0	39.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	2.0
19	2.0
20	11.0
21	55.0
22	157.0
23	290.0
24	683.0
25	1308.0
26	2576.0
27	5678.0
28	12380.0
29	25059.0
30	48076.0
31	85279.0
32	143449.0
33	240815.0
34	428718.0
35	496889.0
36	761400.0
37	1177306.0
38	1733745.0
39	3060102.0
40	4071011.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	53.498086578821145	15.171476714138837	31.33043670704001
2	22.46546216008419	26.7110847480759	32.62188925199062	18.20156383984928
3	22.39681633002963	24.320957319780497	27.088197610197906	26.194028739991964
4	18.153633759693886	28.13982081974345	30.460432145020334	23.246113275542328
5	15.831525890254674	29.959943856820416	30.0965279118093	24.112002341115605
6	17.416073356040137	31.38062787022327	27.510231197366274	23.693067576370318
7	18.2906244842921	32.30727337543014	23.61524727992932	25.786854860348434
8	19.72479366194027	28.377088136609757	29.54070815656422	22.35741004488575
9	19.031942515136038	31.834219019075487	28.254688717594227	20.879149748194244
10	23.472839716134853	25.275524760363833	26.954077973041546	24.29755755045977
11	19.26755875338847	28.093411683926945	27.61080872514527	25.028220837539312
12	18.96315841741431	27.136143957137676	28.492720573326068	25.40797705212195
13	18.260677334261192	28.5903700799179	25.92500052663714	27.223952059183766
14	15.474486240049098	29.301334291121595	27.58562774293568	27.638551725893624
15	16.9034337799135	33.899734631813125	29.448288421148348	19.748543167125025
16	16.23037930969135	38.97685016982116	23.868911515443724	20.92385900504376
17	19.5853547862939	37.852384299852794	22.694994620981078	19.86726629287223
18	17.49522752879973	30.96315711607156	25.63543549801126	25.906179857117444
19	18.713268075874463	27.4599912338299	24.817273177788714	29.00946751250692
20	17.588574470924872	26.348912927400608	30.795267634556605	25.267244967117914
21	17.68136020898914	34.1847693609911	26.92906779206788	21.20480263795189
22	19.40403707427894	27.382089603467037	25.50878231488216	27.705091007371863
23	22.169195216296586	26.481869489474292	30.085149296140308	21.263785998088817
24	18.224565072952867	30.517333356757504	26.820153537297664	24.437948032991965
25	17.88368701065845	30.477990540688253	25.554710812296776	26.08361163635652
26	18.74741493446475	34.02231041475131	27.671589018813464	19.55868563197047
27	20.35941110798024	29.548653584376172	25.20696739997486	24.884967907668724
28	18.600523167366507	27.687596938257503	26.650787846470436	27.061092047905554
29	20.188479729323298	29.861189412711038	27.713323634306015	22.237007223659653
30	20.44236087010251	28.3227096835953	30.359670345266252	20.875259101035937
31	19.064615744205483	32.31680502339099	24.835349649122602	23.78322958328093
32	17.77311973554117	30.70386556426774	27.87082958682471	23.652185113366382
33	18.292311464018518	26.855444570167393	32.63084291790464	22.22140104790945
34	18.4071944731311	32.821740076516406	27.152146321531223	21.618919128821272
35	17.497767893549174	30.503245082043833	25.480211988292556	26.51877503611444
36	16.888595713568495	30.530660716470102	27.79309658888795	24.787646981073454
37	16.524707840416454	36.052063420479456	23.513874189271437	23.90935454983265
38	21.979146711476165	29.879098349398202	24.638192644108447	23.503562295017183
39	17.965571655447913	31.016833950793476	29.721557945087667	21.296036448670943
40	17.77248002388175	30.62462139286814	26.881280024736704	24.721618558513402
41	17.794827016802937	30.773383004913786	27.456882871239426	23.97490710704385
42	17.06359306192306	31.8265636756743	26.625336870679046	24.484506391723592
43	17.859562896732466	28.05163133563213	26.71422729377115	27.374578473864254
44	19.183241438320316	29.428907772100366	24.267597335348235	27.12025345423108
45	17.392676971825793	29.82172221305488	31.604102796835686	21.18149801828364
46	18.004584981009966	31.971913325284362	27.570771479267382	22.45273021443829
47	18.082384657314964	32.79256037143071	25.872750767876575	23.25230420337775
48	20.419726939037737	30.17524794544923	24.872596626241243	24.532428489271794
49	17.45463543957283	30.697575635867036	23.798485259047375	28.04930366551276
50	17.875932921231986	32.79427304857699	26.93908449225425	22.390709537936775
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	117030.0
1	114826.5
2	124895.0
3	134027.0
4	122908.0
5	79723.5
6	31196.5
7	19347.5
8	21518.5
9	22437.5
10	22822.0
11	23069.0
12	22809.5
13	22022.0
14	23522.0
15	27342.0
16	30979.5
17	34438.5
18	39433.0
19	48582.5
20	58524.5
21	69123.5
22	82812.5
23	113417.5
24	149052.5
25	183682.0
26	222072.0
27	256249.0
28	279176.5
29	297625.0
30	335565.5
31	412292.5
32	531575.0
33	614008.5
34	731729.5
35	915410.0
36	940593.5
37	853792.5
38	823157.0
39	803065.0
40	803637.0
41	884031.0
42	956210.0
43	994033.5
44	1016141.0
45	1009657.5
46	1035919.5
47	1019170.0
48	1021950.5
49	1085770.0
50	1165758.5
51	1186800.5
52	999704.0
53	738273.0
54	596883.0
55	508796.0
56	421665.5
57	359806.0
58	289613.5
59	217754.5
60	154707.5
61	108218.5
62	74178.5
63	54222.5
64	38051.5
65	29256.5
66	20523.0
67	12089.5
68	8073.0
69	5905.0
70	4315.0
71	3849.0
72	3584.0
73	2751.0
74	1868.5
75	1069.0
76	543.5
77	424.5
78	311.5
79	202.0
80	105.5
81	50.0
82	16.5
83	12.5
84	9.0
85	7.0
86	6.0
87	2.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	36173.0
25	39594.0
26	46536.0
27	58020.0
28	61915.0
29	60329.0
30	81428.0
31	141151.0
32	295764.0
33	40195.0
34	29862.0
35	32749.0
36	35948.0
37	49627.0
38	29105.0
39	27939.0
40	37282.0
41	36873.0
42	47837.0
43	72879.0
44	210341.0
45	594396.0
46	1513131.0
47	965928.0
48	2561249.0
49	1355219.0
50	3833523.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.61763520131469
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.11030930040907	7.44405688003084
2	12.495588020047792	2.6534719044557113
3	5.054588062293215	1.6100331641504835
4	2.5776814453380754	1.094755250071891
5	1.5741383683291528	0.8356813475655841
6	1.0194162402310791	0.6494273854241627
7	0.7376955892351365	0.5482807859282296
8	0.5591984168359246	0.4749891836093246
9	0.48732975439099263	0.46568605993828605
>10	3.4440864096819643	7.041657768582857
>50	0.4147061390769958	3.1381045894722868
>100	1.2385904714026685	36.40333693055683
>500	0.229748916779603	15.6207260797572
>1k	0.05006468932809259	9.84714461898198
>5k	0.0037719971411576607	2.840032717750172
>10k+	0.0030861794791289953	9.332615333724231
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	106296	0.8645470558624962	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACA	65372	0.5316961140197477	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	56981	0.4634488201823295	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	47249	0.38429464742273545	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAA	35988	0.29270451800989233	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	26610	0.2164295660843402	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACAT	22605	0.1838553303771706	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	22036	0.17922743022301843	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAG	20506	0.16678334017758287	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT	14783	0.12023593669390459	No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACAC	12582	0.10233434049128778	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12521	0.10183820356790768	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.024001640342536185	0.0	0.0	0.0	0.0
2	0.04152910050457125	0.0	0.0	0.0	0.0
3	0.06458726735346657	0.0	0.0	0.0	0.0
4	0.09498175395463829	0.0	0.0	0.0	0.0
5	0.11937379712212931	0.0	0.0	0.0	0.0
6	0.18369266253343944	0.0	0.0	0.0	0.0
7	0.2191786526433972	0.0	0.0	0.0	0.0
8	0.2643189792788007	0.0	0.0	0.0	0.0
9	0.31564068397598927	0.0	0.0	0.0	0.0
10	0.3504272023579029	0.0	0.0	0.0	0.0
11	0.3890038814987532	0.0	0.0	0.0	0.0
12	0.4297033760002954	0.0	0.0	0.0	0.0
13	0.4686704579661005	0.0	0.0	0.0	0.0
14	0.5079466088349949	0.0	0.0	0.0	0.0
15	0.5478408975100677	0.0	0.0	0.0	0.0
16	0.5934692276766648	0.0	0.0	0.0	0.0
17	0.6490446964874238	0.0	0.0	0.0	0.0
18	0.6868568367627375	0.0	0.0	0.0	0.0
19	0.7286787393860249	0.0	0.0	0.0	0.0
20	0.7763241508148886	0.0	0.0	0.0	0.0
21	0.8171049792382964	0.0	0.0	0.0	0.0
22	0.8492969455126977	0.0	0.0	0.0	0.0
23	0.886913884375534	0.0	0.0	0.0	0.0
24	0.9239777525696843	0.0	0.0	0.0	0.0
25	0.9614889573340952	0.0	0.0	0.0	0.0
26	1.001920049893481	0.0	0.0	0.0	0.0
27	1.0356736274677016	0.0	0.0	0.0	0.0
28	1.0719729567963154	0.0	0.0	0.0	0.0
29	1.1142747295586097	0.0	0.0	0.0	0.0
30	1.148792845998367	0.0	0.0	0.0	0.0
31	1.184457770736429	0.0	0.0	0.0	0.0
32	1.2183252158012616	0.0	0.0	0.0	0.0
33	1.2539901405393237	0.0	0.0	0.0	0.0
34	1.2924122852286293	0.0	0.0	0.0	0.0
35	1.3259137276450665	0.0	0.0	0.0	0.0
36	1.355462341458836	0.0	0.0	0.0	0.0
37	1.3759991567298981	0.0	0.0	0.0	0.0
38	1.3964302379025348	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCACGC	170	0.0	111.33689	44
AGTCCCG	170	0.0	107.49769	44
GGTGCGG	90	0.0	101.5256	44
GATCCGA	130	0.0	100.40993	44
ATTGACG	140	0.0	97.89968	44
CTCGACT	140	0.0	93.23779	44
GTTCGGC	695	0.0	88.6616	43
TATCGCA	225	0.0	87.72265	43
ACGAACA	645	0.0	83.98629	44
GTTACGC	75	0.0	83.44349	43
GGGTGCG	85	0.0	79.2902	43
CGAGAGT	110	0.0	78.77533	43
AATAACG	140	0.0	74.59023	44
ACTCGAC	130	0.0	74.06226	43
CGACTAG	40	8.264033E-8	72.21072	43
GAACACA	21775	0.0	71.96544	44
CCTTAGC	31425	0.0	71.157524	43
GCGTCGA	150	0.0	70.60603	43
CGGAAGG	205	0.0	70.042046	44
CTATCAT	17430	0.0	68.973495	44
>>END_MODULE