FastQCFastQC Report
Tue 24 May 2016
ERR841119.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841119.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3260943
Sequences flagged as poor quality0
Sequence length24-50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA419791.2873270093957483No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA404521.2405000639385602No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA390251.1967397160882602No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA352291.0803316709307706No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA291310.8933305488627064No Hit
AAAAAAAAAAAAAAAAAAAAAAAA278580.8542927613270148No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA183830.5637326380743238No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC143970.4414980574637459No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT128450.39390446260483547No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG124790.3826807153636233No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA109290.33514845245685065No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA101870.31239429821373754No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA96750.2966933184664681No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG91980.2820656478816097No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA84360.25869817411711893No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG80760.24765842273232005No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC78900.24195455118350734No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA76830.23560669413724805No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA74020.22698955486189115No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC72060.2209790235523896No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC70860.21729910642412334No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA69530.21322053160696156No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA63760.19552626341521454No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG62410.191386356645915No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA61460.18847308891937087No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG60230.18470117386289794No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGA54570.1673442314079087No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA52560.1611803702180627No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52320.1604443867924094No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50710.15550716464531886No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT50300.15424985962649454No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA48470.14863798600588846No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCC46350.14213679907928473No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA46130.14146214760576925No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT45430.13931552928094726No Hit
TCTCCCAGACAGTGGCTCAGCCTTCAGGACTCTCTTCCAGCATGGCCG44640.1368929171715053No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT44120.13529828641592323No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGA44110.13526762043985435No Hit
AGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGGGC43270.13269167845006796No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA40460.12407453917471112No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT39940.12247990841912906No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCCC38160.11702136467886742No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC37260.11426142683266773No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAG36740.11266679607708568No Hit
TAAAAAAAAAAAAAAAAAAAAAAA35600.10917087480523271No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC34500.10579761743765531No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34320.10524562986841536No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAG33440.10254702397435343No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAC100.0020907288218.09944
CACGTAT253.2359821E-9218.09944
GGAATAC450.0218.0989844
GATAAGT1450.0218.0989844
CACGACA450.0218.0989844
TACGGGT152.415871E-5218.0989844
GCCTATT650.0218.0989844
GATTTAG1500.0210.8290344
ATAACGA1250.0209.3750344
ATAGGGT1200.0209.0115444
TACGTAA1150.0208.6164244
GACCGCA500.0196.289144
GGATGTC800.0190.8366244
CTAAGGG700.0186.9419944
GACCCAT700.0186.9419944
TCCGTTG309.622454E-9181.7491644
ATCACGA1700.0179.6109344
GAGGTCC1250.0174.479244
TACTCCC600.0163.5742544
CTATCAT15800.0163.5742344
Produced by FastQC (version 0.11.5)