##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR841119.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3260943
Sequences flagged as poor quality	0
Sequence length	24-50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.448730627919595	39.0	35.0	39.0	31.0	39.0
2	37.202880271136294	39.0	37.0	39.0	33.0	39.0
3	37.399826675903256	39.0	37.0	39.0	34.0	39.0
4	37.88928018674353	39.0	37.0	39.0	35.0	41.0
5	38.67594343108726	40.0	37.0	41.0	35.0	41.0
6	38.69420195323868	40.0	37.0	41.0	35.0	41.0
7	38.72358425154932	40.0	37.0	41.0	35.0	41.0
8	38.615204252266906	40.0	37.0	41.0	35.0	41.0
9	38.56203282302083	40.0	37.0	41.0	35.0	41.0
10	38.55618880796138	40.0	37.0	41.0	35.0	41.0
11	38.47307603966092	40.0	37.0	41.0	34.0	41.0
12	38.388242910102996	40.0	36.0	41.0	34.0	41.0
13	38.27545988997661	40.0	36.0	41.0	34.0	41.0
14	38.21022845232192	39.0	36.0	41.0	34.0	41.0
15	37.83587262948172	39.0	35.0	41.0	34.0	41.0
16	37.79720375363814	39.0	35.0	41.0	34.0	41.0
17	37.866822572489	39.0	35.0	41.0	34.0	41.0
18	37.84495926485069	39.0	35.0	41.0	34.0	41.0
19	37.82585712169762	40.0	35.0	41.0	34.0	41.0
20	37.781152568444156	40.0	35.0	41.0	34.0	41.0
21	37.751912560262475	39.0	35.0	41.0	34.0	41.0
22	37.75129096092756	39.0	35.0	41.0	34.0	41.0
23	37.709682137958254	39.0	35.0	41.0	34.0	41.0
24	37.52131821991369	39.0	35.0	41.0	33.0	41.0
25	37.51327285804929	39.0	35.0	41.0	33.0	41.0
26	37.481801643779896	39.0	35.0	41.0	33.0	41.0
27	37.527819116985874	39.0	35.0	41.0	33.0	41.0
28	37.56677582013525	40.0	35.0	41.0	33.0	41.0
29	37.59781074057666	39.0	35.0	41.0	33.0	41.0
30	37.65421441578007	39.0	35.0	41.0	34.0	41.0
31	37.73418303768423	39.0	35.0	41.0	34.0	41.0
32	37.56562930449639	39.0	35.0	41.0	33.0	41.0
33	37.52528747309991	39.0	35.0	41.0	33.0	41.0
34	37.541423899643995	39.0	35.0	41.0	33.0	41.0
35	37.60394214613612	39.0	35.0	41.0	34.0	41.0
36	37.598779147598265	39.0	35.0	41.0	34.0	41.0
37	37.557674484089965	39.0	35.0	41.0	34.0	41.0
38	37.50424073037532	39.0	35.0	41.0	34.0	41.0
39	37.42818969146044	38.0	35.0	41.0	34.0	41.0
40	37.41165645293785	38.0	35.0	41.0	34.0	41.0
41	37.31255148769025	38.0	35.0	41.0	34.0	41.0
42	37.208532279343316	38.0	35.0	41.0	34.0	41.0
43	37.099783293434825	38.0	35.0	40.0	33.0	41.0
44	36.995683159051545	37.0	35.0	40.0	33.0	41.0
45	36.7877824419875	37.0	35.0	40.0	33.0	41.0
46	36.57757490636704	37.0	35.0	40.0	32.0	41.0
47	36.32294256956779	37.0	35.0	40.0	32.0	41.0
48	36.31049937637159	37.0	35.0	40.0	32.0	41.0
49	36.378284380152536	37.0	34.0	40.0	32.0	41.0
50	36.490494443323655	37.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	5.0
20	11.0
21	41.0
22	84.0
23	219.0
24	585.0
25	1109.0
26	2286.0
27	4580.0
28	8271.0
29	15357.0
30	26520.0
31	45586.0
32	76858.0
33	150850.0
34	367473.0
35	181910.0
36	283784.0
37	415717.0
38	409420.0
39	550561.0
40	719713.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	53.04413477941811	21.045446056554805	25.910419164027093
2	23.269097313261838	27.790182165097644	28.109077650237985	20.831642871402536
3	21.63843403579885	29.90156528341649	26.34483338101893	22.11516729976574
4	19.34802294918985	30.833289634317435	28.960579807742732	20.858107608749986
5	14.506693309266675	31.949377833344528	32.20332278117097	21.34060607621783
6	18.80848576623388	35.32162322371167	25.78281803760446	20.08707297244999
7	22.668258844144162	33.436708338661546	23.6196094197292	20.27542339746509
8	22.849280100878794	32.74911582324499	25.18102892322865	19.220575152647562
9	19.96502238769583	34.79337111994904	25.207156334839336	20.03445015751579
10	22.438724013268555	30.80480094254944	26.42864349361519	20.327831550566813
11	22.40247682955513	32.750986447785195	23.907010947446796	20.939525775212875
12	23.04232242023243	33.48102067408109	24.814079853588364	18.66257705209812
13	21.904860035885328	32.039382473106706	23.55613084926661	22.49962664174136
14	19.71405203954807	33.17653819769312	24.923097398513253	22.18631236424556
15	19.611474349597646	35.88737981620654	26.249952851061792	18.251192983134022
16	20.278030005430946	37.060200070961066	22.99365551621111	19.668114407396878
17	20.827533630609306	38.906690487996876	22.002776497473278	18.26299938392054
18	20.08333172336959	34.38465499090294	22.903712208401068	22.628301077326405
19	23.02861472892964	31.040468968638823	23.170046210559338	22.7608700918722
20	24.26555753964421	30.577535393902927	25.584777164151596	19.572129902301267
21	21.725678737714826	34.542094112040594	25.21160290136933	18.520624248875247
22	23.339169068579242	34.767795695907594	21.615097228010423	20.277938007502737
23	25.61850973782737	31.57203299781689	23.494584235296355	19.314873029059385
24	20.451998087669732	35.92089772804983	23.828015393093345	19.79908879118709
25	21.78020221864595	33.45040706755539	24.704593985796578	20.064796728002086
26	20.046456721687058	34.90927116648634	25.10877768297895	19.93549442884765
27	23.2893266612878	33.82476836446821	23.831870676333935	19.054034297910054
28	24.292334945594078	30.195212570658715	24.54069142470318	20.971761059044034
29	23.6458349246116	31.067183344111594	25.089578357936617	20.19740337334019
30	25.964172280797854	29.80541872006465	24.735751786902043	19.494657212235452
31	23.15654399435178	30.50011472950313	25.88251698879181	20.46082428735328
32	22.231956942656833	28.185593890836437	29.060923567143725	20.521525599363002
33	22.155960506506823	25.143537974157436	29.753525874888016	22.94697564444773
34	22.660055328960617	27.858310007140897	26.51261100530626	22.969023658592224
35	19.926994253493294	27.98486453490086	27.556292519989267	24.531848691616577
36	22.84968690233705	26.592175966954507	27.57656640599541	22.981570724713027
37	20.013108093965684	31.35040472463591	24.61776843170427	24.01871874969413
38	23.220844742296112	29.07874831702041	25.109147845029728	22.59125909565375
39	21.190416815172703	30.03754308572964	25.81454376098138	22.95749633811627
40	19.72565431620851	30.425837050764848	26.032053988231223	23.816454644795417
41	21.6577475593255	30.8416770040991	25.954391720453867	21.54618371612153
42	19.654157599146778	32.93605780197153	25.215655419859562	22.194129179022127
43	21.597592339176266	27.046408765526138	28.011197735903874	23.344801159393718
44	22.591991884543475	30.347442982430273	24.606268639697713	22.45429649332854
45	20.915428473159782	28.27017768778085	27.92273232403294	22.89166151502642
46	23.34465846263599	28.267924023542	25.18739967897271	23.200017834849294
47	21.59271031337966	29.247971913205156	26.799621908530945	22.359695864884234
48	22.9583668829631	30.160627733308072	25.571227837509277	21.30977754621955
49	21.291705935166544	30.589185501605133	24.88396042106985	23.235148142158476
50	21.556571087740224	30.808108274053346	27.177103160332262	20.458217477874165
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	317320.0
1	313131.5
2	189687.5
3	69904.5
4	67462.0
5	49917.5
6	22312.0
7	10739.5
8	10441.0
9	8429.0
10	6298.0
11	5503.0
12	5465.5
13	4902.0
14	4752.0
15	5337.0
16	5968.0
17	7377.0
18	10710.5
19	14329.0
20	15357.0
21	14785.5
22	16125.5
23	21320.5
24	26057.5
25	30312.0
26	35538.0
27	40761.5
28	46776.5
29	55397.5
30	67303.0
31	80464.5
32	97526.5
33	108829.0
34	116217.5
35	131790.0
36	144458.5
37	142436.0
38	137544.0
39	145636.0
40	158278.0
41	166237.0
42	178962.5
43	191266.0
44	201268.0
45	198294.0
46	206982.0
47	248254.5
48	270071.5
49	282320.0
50	312790.0
51	317776.5
52	298392.5
53	265555.0
54	218658.5
55	189215.5
56	174291.5
57	157631.0
58	136079.5
59	121519.5
60	109759.0
61	86602.5
62	62830.0
63	46362.0
64	32544.5
65	22949.5
66	16047.5
67	11103.0
68	8363.0
69	6089.5
70	4748.0
71	3630.0
72	2801.0
73	2212.5
74	1505.5
75	1401.0
76	1032.0
77	480.5
78	224.0
79	110.5
80	36.5
81	16.5
82	8.0
83	6.5
84	3.0
85	1.0
86	0.0
87	0.0
88	1.0
89	2.0
90	3.0
91	3.5
92	2.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
24	52139.0
25	61789.0
26	62104.0
27	69890.0
28	69276.0
29	57646.0
30	55349.0
31	66021.0
32	59954.0
33	15237.0
34	11475.0
35	10289.0
36	13400.0
37	12254.0
38	9547.0
39	10001.0
40	13511.0
41	15717.0
42	20895.0
43	31154.0
44	89384.0
45	211111.0
46	317340.0
47	341985.0
48	507549.0
49	505891.0
50	570035.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.546488444795337
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.23776194962012	4.663571854362691
2	10.945522572699657	1.4330947408884966
3	4.32869416353229	0.8501323896785149
4	2.399476065672618	0.6283256934995509
5	1.420563302854395	0.4649850623618298
6	0.9938321568187268	0.3903666438407893
7	0.6955571134463303	0.31874196241101205
8	0.5545280351110288	0.29041690993355307
9	0.44330282359646295	0.2611869130997451
>10	3.973720568387693	5.508709225473329
>50	0.7631958194592964	3.57087219341524
>100	1.5169449154161554	25.741279342952577
>500	0.5374688474483331	24.43242390268096
>1k	0.17401859075570453	17.66694453351013
>5k	0.009446723498166816	4.245577863889113
>10k+	0.0059663516830527254	9.53337076800247
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA	41979	1.2873270093957483	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA	40452	1.2405000639385602	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA	39025	1.1967397160882602	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	35229	1.0803316709307706	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29131	0.8933305488627064	No Hit
AAAAAAAAAAAAAAAAAAAAAAAA	27858	0.8542927613270148	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18383	0.5637326380743238	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC	14397	0.4414980574637459	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCAT	12845	0.39390446260483547	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCG	12479	0.3826807153636233	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAGAA	10929	0.33514845245685065	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10187	0.31239429821373754	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGACA	9675	0.2966933184664681	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAG	9198	0.2820656478816097	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA	8436	0.25869817411711893	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGG	8076	0.24765842273232005	No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGAAC	7890	0.24195455118350734	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAA	7683	0.23560669413724805	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAA	7402	0.22698955486189115	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC	7206	0.2209790235523896	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC	7086	0.21729910642412334	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6953	0.21322053160696156	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCTA	6376	0.19552626341521454	No Hit
TAGCTGTTAGTTGGTAGAGGGAAATTCAGGCTACCGTCGCGAAACCTG	6241	0.191386356645915	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAA	6146	0.18847308891937087	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG	6023	0.18470117386289794	No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGA	5457	0.1673442314079087	No Hit
CAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACA	5256	0.1611803702180627	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5232	0.1604443867924094	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5071	0.15550716464531886	No Hit
CCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTCT	5030	0.15424985962649454	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA	4847	0.14863798600588846	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCC	4635	0.14213679907928473	No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATGAA	4613	0.14146214760576925	No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT	4543	0.13931552928094726	No Hit
TCTCCCAGACAGTGGCTCAGCCTTCAGGACTCTCTTCCAGCATGGCCG	4464	0.1368929171715053	No Hit
CTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGCAGATTTT	4412	0.13529828641592323	No Hit
CCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGA	4411	0.13526762043985435	No Hit
AGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTCCGGGC	4327	0.13269167845006796	No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA	4046	0.12407453917471112	No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT	3994	0.12247990841912906	No Hit
CGGCACAGCTGGTTTGAGCAACTGAACTGGAAACAAGATGCAGGACCCC	3816	0.11702136467886742	No Hit
TGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTTC	3726	0.11426142683266773	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAG	3674	0.11266679607708568	No Hit
TAAAAAAAAAAAAAAAAAAAAAAA	3560	0.10917087480523271	No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC	3450	0.10579761743765531	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3432	0.10524562986841536	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAG	3344	0.10254702397435343	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.2354533642569036	0.0	0.0	0.0	0.0
2	0.3448389008946185	0.0	0.0	0.0	0.0
3	0.43462887882431556	0.0	0.0	0.0	0.0
4	0.5263201472702834	0.0	0.0	0.0	0.0
5	0.6086889589913101	0.0	0.0	0.0	0.0
6	0.7312915313147148	0.0	0.0	0.0	0.0
7	0.8109310711656107	0.0	0.0	0.0	3.066597606888559E-5
8	0.8896199657583711	0.0	0.0	0.0	3.066597606888559E-5
9	0.9684008582793382	0.0	0.0	0.0	3.066597606888559E-5
10	1.0466910951832031	0.0	0.0	0.0	3.066597606888559E-5
11	1.1232947034032794	0.0	0.0	0.0	3.066597606888559E-5
12	1.1887052303582124	0.0	0.0	0.0	3.066597606888559E-5
13	1.2535024377917676	0.0	0.0	0.0	3.066597606888559E-5
14	1.3065852423670086	0.0	0.0	0.0	3.066597606888559E-5
15	1.3527375363506813	0.0	0.0	0.0	3.066597606888559E-5
16	1.3940752720915393	0.0	0.0	0.0	3.066597606888559E-5
17	1.443723487347065	0.0	0.0	0.0	3.066597606888559E-5
18	1.4788973618980767	0.0	0.0	0.0	3.066597606888559E-5
19	1.5134579169277107	0.0	0.0	0.0	3.066597606888559E-5
20	1.5506250799232002	0.0	0.0	0.0	3.066597606888559E-5
21	1.583131014556219	0.0	0.0	0.0	3.066597606888559E-5
22	1.6112823805874559	0.0	0.0	0.0	3.066597606888559E-5
23	1.636428480963942	0.0	0.0	0.0	3.066597606888559E-5
24	1.6671864549610342	0.0	0.0	0.0	3.066597606888559E-5
25	1.6946325035426868	0.0	0.0	0.0	3.066597606888559E-5
26	1.722446543837166	0.0	0.0	0.0	3.066597606888559E-5
27	1.7489419471606833	0.0	0.0	0.0	3.066597606888559E-5
28	1.7762959978141293	0.0	0.0	0.0	3.066597606888559E-5
29	1.8054593410556394	0.0	0.0	0.0	3.066597606888559E-5
30	1.8322614041398455	0.0	0.0	0.0	3.066597606888559E-5
31	1.8607500959078402	0.0	0.0	0.0	3.066597606888559E-5
32	1.887552158992046	0.0	0.0	0.0	3.066597606888559E-5
33	1.9133729108420479	0.0	0.0	0.0	3.066597606888559E-5
34	1.944161550815209	0.0	0.0	0.0	3.066597606888559E-5
35	1.9716689313489995	0.0	0.0	0.0	3.066597606888559E-5
36	1.9924911290997727	0.0	0.0	0.0	3.066597606888559E-5
37	2.0080081129906286	0.0	0.0	0.0	3.066597606888559E-5
38	2.0212558146523874	0.0	0.0	0.0	3.066597606888559E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTAC	10	0.0020907288	218.099	44
CACGTAT	25	3.2359821E-9	218.099	44
GGAATAC	45	0.0	218.09898	44
GATAAGT	145	0.0	218.09898	44
CACGACA	45	0.0	218.09898	44
TACGGGT	15	2.415871E-5	218.09898	44
GCCTATT	65	0.0	218.09898	44
GATTTAG	150	0.0	210.82903	44
ATAACGA	125	0.0	209.37503	44
ATAGGGT	120	0.0	209.01154	44
TACGTAA	115	0.0	208.61642	44
GACCGCA	50	0.0	196.2891	44
GGATGTC	80	0.0	190.83662	44
CTAAGGG	70	0.0	186.94199	44
GACCCAT	70	0.0	186.94199	44
TCCGTTG	30	9.622454E-9	181.74916	44
ATCACGA	170	0.0	179.61093	44
GAGGTCC	125	0.0	174.4792	44
TACTCCC	60	0.0	163.57425	44
CTATCAT	1580	0.0	163.57423	44
>>END_MODULE