FastQCFastQC Report
Tue 24 May 2016
ERR841118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4496208
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC117070.26037496485927697No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA114990.2557488443595136No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG114440.25452559134274927No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC89240.19847836221100093No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC70860.1575994704871305No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT69210.15392971143683742No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT60550.13466903666378424No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA50490.11229462693896723No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT100.0021784182215.1316244
GTCACGC152.5519861E-5215.1316244
TACGGAC900.0203.1798744
ACCTTGC2050.0199.3902944
GATGGTA1500.0193.6184744
ATGTACG1350.0191.2281244
GTGTATC800.0188.2401744
AGGTAAG900.0167.324644
TTACCGC1450.0163.2033144
CCACGCG208.0356236E-5161.3487144
CAAAGCG2150.0160.0979544
TTCTACG1950.0143.4210844
AACAACG750.0143.4210844
CTATCAT27650.0138.104444
TCGCGTT2050.0136.4249444
CGAAATA1650.0123.8636644
CACGTCA354.8437323E-6122.93236544
TAATCGC1000.0118.32239544
ACGAATT1550.0117.975444
TGTGAAC3450.0112.24258444
Produced by FastQC (version 0.11.5)