##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR841118.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4496208 Sequences flagged as poor quality 0 Sequence length 24-50 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.48771765007313 39.0 38.0 39.0 35.0 39.0 2 37.94677359232491 39.0 38.0 39.0 35.0 39.0 3 38.112530158747106 39.0 38.0 39.0 35.0 39.0 4 38.65350112806169 39.0 38.0 41.0 35.0 41.0 5 39.28245468181187 40.0 39.0 41.0 35.0 41.0 6 39.308346055164705 40.0 39.0 41.0 36.0 41.0 7 39.28831206207542 40.0 39.0 41.0 35.0 41.0 8 39.240220648155066 40.0 39.0 41.0 35.0 41.0 9 39.19727490365214 40.0 39.0 41.0 35.0 41.0 10 39.170993201382146 40.0 39.0 41.0 35.0 41.0 11 39.044763498485835 40.0 38.0 41.0 35.0 41.0 12 38.99351965033646 40.0 38.0 41.0 35.0 41.0 13 38.854956665705856 40.0 38.0 41.0 35.0 41.0 14 38.89522571019846 40.0 38.0 41.0 35.0 41.0 15 38.89203168536687 40.0 38.0 41.0 35.0 41.0 16 38.926611491283325 40.0 38.0 41.0 35.0 41.0 17 39.01288419041112 40.0 38.0 41.0 35.0 41.0 18 38.99429519274909 40.0 38.0 41.0 35.0 41.0 19 39.022089058157455 40.0 38.0 41.0 35.0 41.0 20 39.02899198613587 40.0 38.0 41.0 35.0 41.0 21 39.0376477244825 40.0 38.0 41.0 35.0 41.0 22 39.02139402803429 40.0 38.0 41.0 35.0 41.0 23 39.00291823687872 40.0 38.0 41.0 35.0 41.0 24 38.943763277855474 40.0 38.0 41.0 35.0 41.0 25 38.94387180597661 40.0 38.0 41.0 35.0 41.0 26 38.902787048856595 40.0 38.0 41.0 35.0 41.0 27 38.86206083667312 40.0 38.0 41.0 35.0 41.0 28 38.790434178098614 40.0 38.0 41.0 35.0 41.0 29 38.737905460173856 40.0 38.0 41.0 35.0 41.0 30 38.71413534186335 40.0 38.0 41.0 35.0 41.0 31 38.64764887155345 40.0 38.0 41.0 35.0 41.0 32 38.469833743806 40.0 37.0 41.0 34.0 41.0 33 38.425237426198706 40.0 37.0 41.0 34.0 41.0 34 38.49005963158271 40.0 37.0 41.0 35.0 41.0 35 38.43613491317919 40.0 37.0 41.0 34.0 41.0 36 38.44384724048906 40.0 37.0 41.0 35.0 41.0 37 38.41192469130971 40.0 37.0 41.0 34.0 41.0 38 38.42053546162846 40.0 37.0 41.0 35.0 41.0 39 38.40901641709438 40.0 37.0 41.0 34.0 41.0 40 38.275310710846426 40.0 36.0 41.0 34.0 41.0 41 38.21872479268811 40.0 36.0 41.0 34.0 41.0 42 38.1613441126964 40.0 36.0 41.0 34.0 41.0 43 38.07554413876226 39.0 36.0 41.0 34.0 41.0 44 37.930288203094086 39.0 36.0 41.0 34.0 41.0 45 37.730391001103975 39.0 35.0 41.0 34.0 41.0 46 37.53003918709243 39.0 35.0 41.0 33.0 41.0 47 37.27922914108746 39.0 35.0 41.0 33.0 41.0 48 37.32235843086324 39.0 35.0 41.0 33.0 41.0 49 37.30929441236916 38.0 35.0 40.0 33.0 41.0 50 37.12653821146404 38.0 35.0 40.0 32.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 13.0 21 18.0 22 55.0 23 105.0 24 246.0 25 554.0 26 1036.0 27 2253.0 28 4793.0 29 9663.0 30 19070.0 31 35225.0 32 60159.0 33 112219.0 34 216195.0 35 170539.0 36 255342.0 37 428909.0 38 620074.0 39 1020014.0 40 1539725.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 48.3042377043055 19.013800073306218 32.681962222388286 2 25.12352631372926 22.172550736086944 29.732499030293972 22.971423919889826 3 23.133004522922427 21.143238924889594 29.450661535231465 26.27309501695651 4 21.345097913619654 22.007100205328577 32.38019682363449 24.26760505741727 5 17.36647859707558 24.7175175169832 35.082473942486644 22.83352994345457 6 21.862178084287915 26.643673958144287 31.92276691825645 19.571381039311348 7 23.944377128460246 24.370869852996123 26.81101497083765 24.873738047705977 8 25.70579474970909 23.77968279047589 27.404181479148654 23.110340980666376 9 22.768341678143003 25.37691761591101 27.550504780917606 24.30423592502838 10 24.4350350339664 22.42111574909346 30.07538797137499 23.068461245565153 11 25.473576845199332 23.504628789415435 28.10908659030009 22.91270777508514 12 24.869134168170156 25.309638699988966 27.2792539846911 22.541973147149776 13 23.690518766035733 25.059939397821452 28.44643753135976 22.803104304783055 14 21.77174632490312 25.619766701184645 26.494681740702386 26.113805233209852 15 21.528029842035778 29.517162017415565 27.220938177237354 21.733869963311307 16 23.093971631205672 28.593094447587834 27.192647671104186 21.120286250102307 17 24.151551707572246 30.422013394398125 23.757575272318363 21.668859625711264 18 20.393496030432757 29.557106788653904 23.945400212801545 26.103996968111797 19 26.25906096870963 22.786601509538702 26.61229195802329 24.34204556372837 20 25.26884877212086 24.23597840669293 27.275384056965336 23.219788764220873 21 22.466665243244975 27.547302082110082 26.358455836562722 23.627576838082224 22 27.214354851910766 25.847113834591283 24.35688028667713 22.581651026820822 23 27.327939454758322 26.02862234131517 25.96283356997719 20.68060463394932 24 24.02918192396793 27.84688786639764 24.820003878824114 23.303926330810317 25 25.152585356270624 26.42811856786309 27.908118389270136 20.511177686596152 26 24.528169044086436 27.91746369293559 27.34376173677471 20.210605526203267 27 24.814653037330572 28.177444049173683 26.0212995902193 20.986603323276444 28 24.964669082351428 26.55707875846148 26.078122131449806 22.40013002773729 29 26.331529605274394 29.20943963417398 24.414464048159743 20.044566712391884 30 28.104422279309144 27.765707578907683 25.047206784751282 19.082663357031894 31 25.37940600995357 27.525211580103527 26.18691885785598 20.90846355208692 32 22.142112132863208 27.70377457668161 30.371882327232353 19.78223096322283 33 23.932878448421686 23.775632994162788 31.656240984253074 20.63524757316245 34 24.839327086767238 24.87338433385957 27.744168722983666 22.543119856389527 35 21.95610776084124 24.663026152307648 29.35390769417255 24.026958392678566 36 23.750207024209654 24.132227580495886 29.141287934141946 22.976277461152517 37 21.78741952527249 28.676154041837076 27.4008130638123 22.135613369078136 38 23.98083727987912 26.92932062866428 26.683271538330477 22.406570553126123 39 23.837353692730257 26.844330470755562 26.344199389504176 22.974116447010005 40 20.833794291934176 25.73752757398664 28.52956310034444 24.899115033734745 41 22.07346681145987 27.43522202314538 28.087636333816253 22.403674831578503 42 22.08300362588715 29.008595407575832 24.642124363859185 24.266276602677827 43 21.438124158799134 26.015839126354116 29.31165124888897 23.234385465957782 44 22.594603057686115 27.511550007586898 26.938834331830442 22.955012602896545 45 21.456006903401434 26.231606797276406 29.581217557890092 22.731168741432064 46 18.910626651511926 28.504306175169376 26.68866460752339 25.896402565795306 47 23.115633886792427 26.09360044897081 28.9305061459108 21.86025951832596 48 23.118841901589395 28.2519881890089 26.62301986619277 22.006150043208926 49 19.731620017394437 28.69072373203855 26.142765038387516 25.434891212179494 50 23.671721861136902 26.474751618885843 28.03437628209281 21.819150237884447 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 35605.0 1 37174.5 2 49284.0 3 59416.5 4 56043.5 5 37584.5 6 14650.5 7 7834.0 8 8747.0 9 9616.0 10 10424.5 11 10272.5 12 9615.0 13 9559.5 14 10427.5 15 11091.5 16 10617.5 17 9232.5 18 9270.5 19 14121.5 20 19820.5 21 20347.5 22 18976.0 23 22689.0 24 28143.0 25 35238.0 26 44194.5 27 53104.5 28 62832.0 29 74119.0 30 86640.5 31 101487.0 32 126466.0 33 147608.0 34 167352.0 35 186459.0 36 190632.0 37 196929.0 38 208094.0 39 220338.0 40 230620.0 41 228101.5 42 236031.0 43 265839.0 44 297021.0 45 314348.5 46 342541.0 47 391632.5 48 425155.5 49 454704.5 50 510305.5 51 527788.5 52 474860.5 53 408305.0 54 351179.0 55 309396.5 56 287636.0 57 267531.5 58 240245.0 59 211126.0 60 169618.0 61 121679.5 62 91266.0 63 73754.5 64 56605.5 65 45212.5 66 33433.5 67 22658.5 68 15437.5 69 9719.0 70 6555.5 71 4402.5 72 3268.5 73 2107.5 74 1215.5 75 805.0 76 492.5 77 310.5 78 182.5 79 106.0 80 85.0 81 63.0 82 43.0 83 27.0 84 20.0 85 16.5 86 14.5 87 13.5 88 12.5 89 13.0 90 8.5 91 3.0 92 2.0 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 24 16748.0 25 19653.0 26 18407.0 27 23881.0 28 26798.0 29 30119.0 30 49587.0 31 78090.0 32 77002.0 33 15835.0 34 15213.0 35 19075.0 36 17560.0 37 20759.0 38 12744.0 39 14244.0 40 16363.0 41 19317.0 42 23154.0 43 47246.0 44 142659.0 45 341128.0 46 480277.0 47 549553.0 48 767394.0 49 819804.0 50 833598.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.138810849885182 #Duplication Level Percentage of deduplicated Percentage of total 1 73.95866203397993 9.71728871175047 2 11.157481171834624 2.9319206935578084 3 4.379610117431223 1.7262860678751681 4 2.285149922345795 1.2009661037332486 5 1.264994067820131 0.8310258891657766 6 0.8639146185875248 0.6810486458443314 7 0.6063585743560854 0.5576781430969453 8 0.4674303024633668 0.49131826636566367 9 0.3540935558298804 0.4187131427890851 >10 2.828532001259509 7.514853302849881 >50 0.4638648043907814 4.341078427753668 >100 1.1259478211963774 39.66033409891462 >500 0.2002513278143379 16.4547919974902 >1k 0.04099900281816394 10.414918966978652 >5k 0.0016941736701720637 1.5151992230667102 >10k+ 0.0010165042021032381 1.5425783187677833 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC 11707 0.26037496485927697 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA 11499 0.2557488443595136 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG 11444 0.25452559134274927 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC 8924 0.19847836221100093 No Hit ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC 7086 0.1575994704871305 No Hit TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTT 6921 0.15392971143683742 No Hit CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT 6055 0.13466903666378424 No Hit ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTA 5049 0.11229462693896723 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.03591915676498952 0.0 0.0 0.0 0.0 2 0.0612738556579233 0.0 0.0 0.0 0.0 3 0.08351481959909328 0.0 0.0 0.0 0.0 4 0.12030137395778844 0.0 0.0 0.0 0.0 5 0.1394286029471946 0.0 0.0 0.0 0.0 6 0.2215867237458765 0.0 0.0 0.0 0.0 7 0.24825363951133933 0.0 0.0 0.0 0.0 8 0.28401710952874065 0.0 0.0 0.0 0.0 9 0.328810410906257 0.0 0.0 0.0 0.0 10 0.3561000736620726 0.0 0.0 0.0 0.0 11 0.3918635436794739 0.0 0.0 0.0 0.0 12 0.4263147968243462 0.0 0.0 0.0 0.0 13 0.4590090138178661 0.0 0.0 0.0 0.0 14 0.49234821876567986 0.0 0.0 0.0 0.0 15 0.5321817851843154 0.0 0.0 0.0 0.0 16 0.5658546045912467 0.0 0.0 0.0 0.0 17 0.6099361951226455 0.0 0.0 0.0 0.0 18 0.6380710145082256 0.0 0.0 0.0 0.0 19 0.6679851110090992 0.0 0.0 0.0 0.0 20 0.7092643400839107 0.0 0.0 0.0 0.0 21 0.7355309184984324 0.0 0.0 0.0 0.0 22 0.7612859547423073 0.0 0.0 0.0 0.0 23 0.7880640753274759 0.0 0.0 0.0 0.0 24 0.8149311597684092 0.0 0.0 0.0 0.0 25 0.8444449189183418 0.0 0.0 0.0 0.0 26 0.8721126780611573 0.0 0.0 0.0 0.0 27 0.89744513599015 0.0 0.0 0.0 0.0 28 0.9240675698277304 0.0 0.0 0.0 0.0 29 0.9556052566963095 0.0 0.0 0.0 0.0 30 0.9832952568030661 0.0 0.0 0.0 0.0 31 1.009050293046941 0.0 0.0 0.0 0.0 32 1.0347608473629333 0.0 0.0 0.0 0.0 33 1.0632070402436897 0.0 0.0 0.0 0.0 34 1.0929876909609164 0.0 0.0 0.0 0.0 35 1.117252582620733 0.0 0.0 0.0 0.0 36 1.1424738357300197 0.0 0.0 0.0 0.0 37 1.1592212815777205 0.0 0.0 0.0 0.0 38 1.1729661972933636 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGGGT 10 0.0021784182 215.13162 44 GTCACGC 15 2.5519861E-5 215.13162 44 TACGGAC 90 0.0 203.17987 44 ACCTTGC 205 0.0 199.39029 44 GATGGTA 150 0.0 193.61847 44 ATGTACG 135 0.0 191.22812 44 GTGTATC 80 0.0 188.24017 44 AGGTAAG 90 0.0 167.3246 44 TTACCGC 145 0.0 163.20331 44 CCACGCG 20 8.0356236E-5 161.34871 44 CAAAGCG 215 0.0 160.09795 44 TTCTACG 195 0.0 143.42108 44 AACAACG 75 0.0 143.42108 44 CTATCAT 2765 0.0 138.1044 44 TCGCGTT 205 0.0 136.42494 44 CGAAATA 165 0.0 123.86366 44 CACGTCA 35 4.8437323E-6 122.932365 44 TAATCGC 100 0.0 118.322395 44 ACGAATT 155 0.0 117.9754 44 TGTGAAC 345 0.0 112.242584 44 >>END_MODULE