FastQCFastQC Report
Tue 24 May 2016
ERR841117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR841117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7276080
Sequences flagged as poor quality0
Sequence length24-50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAA320800.4408967465998175No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAA309880.425888665325285No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAA294710.4050395267781553No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCCAG284350.39080109069718855No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA250500.3442787874789722No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAA239300.32888588360765686No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA211820.29111829446625104No Hit
AAAAAAAAAAAAAAAAAAAAAAAA187520.25772119053116516No Hit
ACCGCGGCCTCAGATGAATGCGGCTGTTAAGACCTGCAATAATCC168300.2313058679948544No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA154230.2119685325065145No Hit
TCAGTTCTTTGACCATTCGTTGGAGCTCCGGTTTTCAGCCTCTTTC119160.1637695022594584No Hit
CTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCT111310.15298072588536685No Hit
CTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGAACGA109070.14990214511110378No Hit
AGGTTTTCTGTTTCCTGTGATACCTTGGGCTGGAATCTGAAGCAATG94190.1294515728249277No Hit
ATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATC89080.12242856043364009No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA87460.12020208683796771No Hit
CCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA80420.11052654726171234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTACG900.0202.9655844
CCCGTTG1600.0167.894344
ATCTCGT700.0153.5033744
TCGCACA650.0148.7801844
ATGACGT1300.0148.7801844
ATCCGTT457.075869E-10143.269844
ACCGACC1000.0118.1975944
GAAACGA2000.0118.1975944
GACGTTC2350.0109.7385744
AGTCCCG650.0108.2721243
CTATCAT34100.0107.45235444
CGTTATT1450.097.0715543
CACCCGC451.697911E-595.5132144
CGGAGTT1850.092.9317744
CGTCGGA2100.092.1020244
GCACCTC2750.089.8692444
ATCGCAC550.088.5862843
AACCGCT1850.087.12353544
CGTTTAA2750.085.9618844
AACGACA30950.085.7081443
Produced by FastQC (version 0.11.5)